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| 1 | Effects of icariin on cGMP-specific PDE5 and cAMP-specific PDE4 activities显示文摘Aim:To clarify the mechanism of the therapeutic action of icariin on erectlile dysfunction(ED).Methods:PDE5 was isolated from the human platelet and PDE4 form the rat liver tissue using the FPLC system (Pharmacia,Milton Keynes,UK)and the Mono Q column.The inhibitory effects of icariin on PDE5 and PDE4 activities were investigated by the two-step radioisotope procedure with [^3H]-c GMP/[^3H]-cAMP.Papaverine served as the control drug.Results:Icariin and papaverine showed dose-dependent inhibitory effects on PDE5 and PDE4 activities.The IC50 of Icariin and papaverine on PDE5 were 0.432μ mol/L and 0.680μmol/L,respectively and those on PDE4,73.50μmol/L and 3.07μmol/L,respectively.The potencies of selectivity of icariin and papaverine on PDE5(PDE4/PDE5 of IC50)were 167.67 times and 4.54 times,respectively.Conclusion:Icariin is a cGMP-specific PDE5 inhibitor that may be developed into an oral effective agent for the treatment of ED. | ZC Xin EK Kim CS Lin WJ Liu L Tian YM Yuan J Fu | 2003 | Asian Journal of Andrology2003，5，1： | 38 |
| 2 | Recognition and implication of eclogite in the western Altun Mountains, Xinjiang显示文摘SINCE high-pressure metapelitic rocks have been discovered in the Beiketan area of eastern Al-tun Mountains,a set of eclogite has been recognized in south of Jianggesayi,western Altun.The eclogite,associated with quartz-garnet-clinopyroxenite and metamorphosed retrogradelyto garnet-amphibolite partially,is exposed in the Proterozoic meso- to hypometamorphic rocksand can be traced eastward to northwest of Mangya asbestos deposit,for about 200 | Liu, L Che, ZC Luo, JH Wang, Y Gao, ZJ | 1997 | Chinese Science Bulletin1997，42，11： | 33 |
| 3 | Down-regulation of Hsp90 could change cell cycle distribution and increase drug sensitivity of tumor cells显示文摘:AIM To construct Hsp90 antisense RNAeukaryotic expression vector, transfect it intoSGC7901 and SGC7901/VCR of MDR-type humangastric cancer cell lines, HCC7402 of humanhepatic cancer and Eel09 of human esophagealcancer cell lines, and to study the cell cycledistribution of the gene transected cells andtheir response to chemotherapeutic drugs.METHODS A I .03kb cDNA sequence of Hsp90Pwas obtained from the primary plasmid phHsp90by EcoR 1 and BamH I nuclease digestion andwas cloned to the EcoR 1 and BamH 1 site ofthe pcDNA by T4DNA ligase and an antisenseorientation of Hsp900 expression vector wasconstructed. The constructs were transfectedwith lipofectamine and positive clones wereselected with G418. The expression of RNA wasdetermined with dot blotting and RNaseprotection assay, and the expression of Hsp90protein determined with Western blot. Cell cycledistribution of the transfectants was analyzedwith flow cytometry, and the drug sensitivity ofthe transfectants to adriamycin (ADR ),vincrinstine (VCR ), mitomycin (MMC ) andcyclophosphamide (CTX ) with MTT andintracellular drug concentration of thetransfectants was determined with flowcytometry.RESULTS In EcoR 1 and BamH I restrictionanalysis, the size and the direction of the clonedsequence of Hsp900 remained what had beendesigned and the gene constructs were namedpcDNA-Hsp90. AH^SGC7901, AH^SGC7901/ VCR,AH-HCC7402 and AH-Eel09 cell clones allexpressed Hsp90 anti--sense RNA. Theexpression of Hsp90 was down--regulated in AHSGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH--Eel09 cell clones. Cell cycle distribution waschanged differently. In AH-SGC7901/ VCR andAH-Ec109 cells, G, phase cells were increased; Sphase and G, phase cells were decreased ascompared with their parental cell lines. In AHSGC7901 cell, G, phase cells were decreased, Qphase cells increased and S phase cells were notchanged, and in AH-HCC7402 cells G,, S and qphase cells remained unchanged as comparedwith their parental cell lines. The sensitivity ofAH--SGC7901, AH--SGC7901/ VCR, AH-HCC7402 andAH-Ec109 to chemotherapeutic drugs, thesensitivity ot AH--SGC7901/ VCR to ADR, VCR,MMC and CTX the sensitivity of AH-HCC7402 toADR and VCR, and the sensitivity of Eel09 toADR, VCR and CTX all increased as comparedwith their parental cell lines. The meanfluorescence intensity of ADR in AH--SGC7901,AH-SGC7901/ VCR, AH--HCC7402 and AH-Ec109was also significantly elevated (P< 0. 05).CONCLUSION Down-regulation of HsP90 couldchange cell cycle distribution and increase thedrug sensitivity of tumor cells. | Liu XL Xiao B Yu ZC Guo JC Zhao QC Xu L Shi YQ Fan DM | 1999 | World Journal of Gastroenterology1999，5，3： | 21 |
| 4 | Protective effect of early enteral feeding on postburn impairment of liver function and its mechanism in rats显示文摘AIM To study the protective effect of earlyenteral feeding(EEF)on the postburnimpairment of liver function and its mechanism.METHODS Wistar rats with 30% of total bodysurface area(TBSA)full-thickness burn wereemployed.The effects of EEF on the postburnchanges of gastric intramucosal pH,endotoxinlevels in portal vein,water contents of hepatictissue,blood concentrations of tumor necrosisfactor(TNF-α),plasma activities of alanineaminotransferase(ALT)and asparateaminotransferase(AST),as well as the bloodcontents of total(TB)and direct bilirubin(DB),total protein(TP)and albumin(ALB)wereserially determined within 48h postburn.RESULTS EEF could significantly improvegastric mucosal acidosis,reduce portal veinendotoxin level and water content of hepatictissue,as well as plasma concentrations of TNF-α at all timepoints after severe burns(P<0.01);postburn elevation of the plasma activities ofALT,AST and the contents of TB,DB wereeffectively prevented,whereas the plasmaconcentrations of TP and ALB were markedlyincreased 24 h and 48 h posturn in EEF groupcompared with that of the burn without EEFgroup(P<0.01).CONCLUSION EEF has significant beneficialeffects on the improvement of hepatic function in rats after severe burn,and is probably relatedwith an increase in splanchnic blood flow,reduction of the absorption of gut-originendotoxin and the consequent release ofinflammatory mediators. | Zhu L Yang ZC Li A Cheng DC | 2000 | World Journal of Gastroenterology2000，6，1： | 10 |
| 5 | Reduced gastric acid production in burn shock period and its significance in the prevention and treatment of acute gastric mucosal lesions显示文摘AIM To investigate the changes of gastric acidproduction and its mechanism in shock period ofsevere burn in rats.METHODS A rat model with 30% TBSA full-thickness burn injury was employed and thegastric acid production,together with gastricmucosal blood flow(GMBF)and energy charge(EC)were measured serially within 48hpostburn.RESULTS The gastric acid production in theacute shock period was markedly inhibited aftersevere burn injury.At the 3rd h postburn,thegastric juice volume,total acidity and acidoutput were already significantly decreased(P<0.01),and reached the lowest point,0.63mL/L±0.20mL/L,10.81mmol/L±2.58mmol/L and 2.23mmol/h±0.73mmol/hrespectively,at the 12th h postburn.Althoughrestored to some degree 24 h after thermalinjury,the variables above were stillstatistically lower,compared with those ofcontrol animals at the 48th h postburn.TheGMBF and EC were also significantly reducedafter severe burns,consistent with the trend ofgastric acid production changes.CONCLUSION Gastric acid production,as wellas GMBF and EC was predominantly decreased in the early postburn stage,suggesting that gastricmucosal ischemia and hypoxia with resultantdisturbance in energy metabolism,but notgastric acid proper,might be the decisive factorin the pathogenesis of AGML after thermalinjury,and that the preventive use of anti-aciddrugs during burn shock period wasunreasonable in some respects.Therefore,taking effective measures to improve gastricmucosal blood perfusion as early as possiblepostburn might be more preferable for the AGMLprevention and treatment. | Zhu L Yang ZC Li A Cheng DC | 2000 | World Journal of Gastroenterology2000，6，1： | 7 |
| 6 | Expression of co-stimulator 4-1BB molecule in hepatocellular carcinoma and adjacent non-tumor liver tissue,and its possible role in tumor immunity显示文摘AIM:To investigate the expression of 4-1BB molecule in hepatocellular carcinoma (HCC) and its adjacent tissues.METHODS:Reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the gene expression of 4-1BB in hepatocarcinoma and its adjacent tissues,and peripheral blood mononuclear cells (PBMCs) from both HCC and health control groups.Flow cytometry was used to analyse the phenotypes of T cell subsets from the blood of HCC patients and healthy volunteers,and further to determine whether 4-1BB molecules were also expressed on the surface of CD4+ and CD8+ T cells. The localization of 4-1BB proteins on tumor infiltrating T cells was determined by direct immunofluorescence cytochemical staining and detected by confocal microscopy.RESULTS:4-1BB mRNA, which was not detectable in normal liver,was found in 19 liver tissues adjacent to tumor edge(<1.0cm).Low expression of 4-1BB mRNA was shown in 8 tumor tissues and 6 liver tissues located within 1 to 5cm away from tumor edge. In PBMCs, 4-1BB mRNA was almost not detected. Percentage of CD4^+, CD8^+ and CD3^+/CD25^+ T cells, as well as ratio of CD4 to CD8 revealed no difference between groups (P>0.05,respectively),while a significant lower percentage of CD3^+ T cell was found in HCC group as compared to healthy control group (P<0.05).However, 4-1BB molecules were almost not found on the surface of CD4+ and CD8+ T cells in HCC and healthy control group.Double-staining of 4-1BB^+/CD4^+ and 4-1BB^+/CD8^+ immunofluorescence on tumor infiltrating T cells was detected in 13 liver tissues adjacent to tumor edge (<1.0cm) by confocal microscopy.CONCLUSION: Although HCC may escape from immune attack by weak immunogenicity or downregulated expression of MHC-1 molecules on the tumor cell surface,tumor infiltrating T cells can be activated via other costimulatory signal pathways to exert a limited antitumor effect on local microenvironment. The present study also implicates that modulating 4-1BB/4-1BBL costimulatory pathway may be an effective immunotherapy strategy to augment the host response. | Wan, YL Zheng, SS Zhao, ZC Li, MW Jia, CK Zhang, H | 2004 | World Journal of Gastroenterology2004，10，2： | 6 |
| 7 | A Novel Electrocatalysis Method for Organic Pollutants Degradation显示文摘A novel electrocatalysis, ferrous ion catalyzed anodic-cathodic electrocatalysis (FACEC), was developed for organic pollutants degradation, which could promote the degradation by achieving synergetic effects of both anodic oxidation and cathodic indirect oxidation. The degradation rate of model pollutants phenol by FACEC could increase by nearly 30% comparing with that of anodic electrocatalysis, and the current efficiency could reach 67%. | Zhou, MH Wu, ZC Wang, DH | 2001 | Chinese Chemical Letters2001，12，10： | 5 |
| 8 | The lipase monolayer film self-assembly on the negatively charged poly(ethylene terephthalate) substrate显示文摘The PET-CO2-film was prepared and the lipase was assembled on the surface of the PET-CO2- substrate The structure at the surface and activity of lipase/PET monolayer were studied by ATR-FTIR and AFM, and other methods. | Lin, XF He, L Xu, LH Lu, DS Chen, ZC | 2000 | Chinese Chemical Letters2000，11，3： | 3 |
| 9 | Intraspinal transplantation of CD34+ human umbilical cord blood cells after spinal cord hemisection injury improves functional recovery in adult rats显示文摘 | Zhao,ZM Li,HJ Liu,HY Lu,SH Yang,RC Zhang,QJ Han,ZC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 10 | Circulating Th1 and Th2 cytokines in patients infected with hepatitis C virus 显示文摘 | Fan XG Liu WE Wang ZC | 1998 | Med Inflamm1998，7，： | 2 |
| 11 | Hemangiopoietin, a novel human growth factor for the primitive cells of both hematopoietic and endothelial cell lineages显示文摘 | Liu,YJ Lu,SH Xu,B Yang,RC Ren,Q Liu,B Lu,M Yan,FY Han,ZB Han,ZC | 2005 | 中国生物学文摘2005，19，3： | 2 |
| 12 | Platelet factor 4modulates fibroblast growth factor 2(FGF2-2) activity and inhibits FGF2-2 dimerization显示文摘 | Perollet C Han ZC Savona C | 1998 | Blood1998，91，： | 2 |
| 13 | THE MORTAR ELEMENT METHOD FOR A NONLINEAR BIHARMONIC EQUATION显示文摘臼元素方法是新领域分解方法(DDM )舔 withnonover 子域。它能处理不同子域上的网孔不必越过接口,并且在邻近的子域上匹配 discretizations 排列的状况仅仅微弱地被强制。但是直到现在,为非线性的 PDE 有很小的工作。在这篇论文,我们将为与著名海军司烧方程有关的一个非线性的双性人泛音方程介绍一个臼类型莫利元素方法。最佳的精力和 H^1 标准估计在一个合理椭圆形的整齐假设下面被获得。 | Shi, ZC Xu, XJ | 2005 | Journal of Computational Mathematics2005，23，5： | 2 |
| 14 | Observation of changes in peripheral T lymphocyte subsets by flow cytometry in patients with liver cancer treated with radioimmunotherapy 显示文摘 | Zeng ZC Tang ZY | 1995 | Nucl Med Commun1995，16，5： | 2 |
| 15 | Discrete Element with Flexible Connector for Dynamic Analysis of 3-D Beam Structures显示文摘Combined multi-body dynamics with structural dynamics,a new discrete element with flexibleconnector,which is applicable for 3-D beam structures,is developed in this paper.Both the generalizedelastic coefficient matrix of the flexible connector and the mass matrix of discrete element may be off-diag-onal in a general case.The zero-length rigid element is introduced to simulate the node at which multiple el-ements are jointed together.It may also be effective when the axes of adjacent elements are not in the sameline.The examples for eigenvalue calculation show that the model is successful.It can be extended to thegeometric nonlinear response analysis. | Cheng, BR Zheng, ZC Hou, ZC | 1997 | China Ocean Engineering1997，12，1： | 2 |
| 16 | Frequent loss of membranous E - cadherin in gastric cancers: A cross - talk with Wnt in determining the fate of beta -catenin显示文摘 | Cheng XX Wang ZC Chen XY | 2005 | Clin Exp Metastasis2005，22，1： | 1 |
| 17 | Polychlorinated dibenzo-p-dioxins and dibenzofurans in paper making from a pulp mill in China显示文摘 | Bao ZC Zhang B | 2004 | Chemosphere2004，44，： | 1 |
| 18 | Mixed-valent oxide-catalytic carbonization for synthesis of monodispersed nano sized catkin spheres显示文摘 | Wang ZL | 1996 | Philosophical magazine β-physics of condensed matter statistical mechanics electronic optical and magnetic properties1996，73，6： | 1 |
| 19 | Increased umbilical cord plasma interleukin-1 beta levels was correlated with adverse outcomes of neonatal hypoxic-ischemic encephalopathy显示文摘 | Liu J Feng ZC | 2010 | J Trop Pediatr2010，56，3： | 1 |
| 20 | Advances in cancer pmteomics study显示文摘 | Chen ZC | 2004 | Ai Zheng2004，23，2： | 1 |
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