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| 1 | A structural view of the antibiotic degradation enzyme NDM-1 from a superbug显示文摘Gram-negative Enterobacteriaceae with resistance to carbapenem conferred by New Delhi metallo-β-lactamase 1(NDM-1)are a type of newly discovered antibioticresistant bacteria.The rapid pandemic spread of NDM-1 bacteria worldwide(spreading to India,Pakistan,Europe,America,and Chinese Taiwan)in less than 2 months characterizes these microbes as a potentially major global health problem.The drug resistance of NDM-1 bacteria is largely due to plasmids containing the blaNDM-1 gene shuttling through bacterial populations.The NDM-1 enzyme encoded by the blaNDM-1 gene hydrolyzes β-lactam antibiotics,allowing the bacteria to escape the action of antibiotics.Although the biological functions and structural features of NDM-1 have been proposed according to results from functional and structural investigation of its homologues,the precise molecular characteristics and mechanism of action of NDM-1 have not been clarified.Here,we report the threedimensional structure of NDM-1 with two catalytic zinc ions in its active site.Biological and mass spectroscopy results revealed that D-captopril can effectively inhibit the enzymatic activity of NDM-1 by binding to its active site with high binding affinity.The unique features concerning the primary sequence and structural conformation of the active site distinguish NDM-1 from other reported metallo-β-lactamases(MBLs)and implicate its role in wide spectrum drug resistance.We also discuss the molecular mechanism of NDM-1 action and its essential role in the pandemic of drug-resistant NDM-1 bacteria.Our results will provide helpful information for future drug discovery targeting drug resistance caused by NDM-1 and related metallo-β-lactamases. | Yu Guo Jing Wang Guojun Niu Wenqing Shui Yuna Sun Honggang Zhou Yaozhou Zhang Cheng Yang Zhiyong Lou Zihe Rao | 2011 | Protein & Cell2011，2，5： | 16 |
| 2 | Reduction of low-grade manganese oxide ore by biomass roasting显示文摘The reduction process of manganese dioxide in low-grade manganese ore by biomass roasting was investigated.The calcine of manganese oxide ore was further leached by sulphuric acid, the manganese in ore can be converted into manganese sulfate.Effects of the mass ratio of manganese ore to sawdust, roasting temperature and time, leaching temperature and time, leaching agent concentration and liquid-solid ratio were studied.97.71% of manganese recovery can be achieved under the optimal conditions:the mass ratio of manganese ore to sawdust of 5:1, roasting temperature at 500℃ for 40 min, leaching temperature at 60℃ for 40 min, sulphuric acid concentration of 1 mol/L and liquid-solid ratio of 10:1.Other types of low-grade manganese ore like Guilin ore, Nanning ore and Gongcheng ore were tested and the same results were obtained. | Jingjing SONG Guocai ZHU Ping ZHANG Yuna ZHAO | 2010 | Acta Metallurgica Sinica(English Letters)2010，23，3： | 14 |
| 3 | Molecular mechanism of SCARB2-mediatec attachment and uncoating of EV71显示文摘 | Minghao Dang Xiangxi Wang Quan Wang Yaxin Wang Jianping Lin Yuna Sun Xuemei Li Liguo Zhang Zhiyong Lou Junzhi Wang Zihe Rao | 2014 | Protein & Cell2014，5，9： | 13 |
| 4 | Recovering indium with sulfating roasting from copper-smelting ash显示文摘A technology for recovering indium from Jinchuan copper-smelting ash was developed. Indium in the ash was first enriched to the leaching-slag in leaching process,and then recovered by sulfating roasting. The method included mix-ing the leaching-slag with sulfuric acid,making them into particles,roasting the mixture,and then leaching the calcine with hot water. Above 90% of indium in calcine could be dissolved into the leaching solution. The optimized conditions were determined as follows: the mass ratio of sulfuric acid to leaching slag was 0.1,the roasting time was about 1 to 1.5 h in the temperature range of 200-250°C,and the calcine was leached for 1 h with 5:1 of liquid/solid ratio at 60°C. Over 99% of in-dium in leaching solution was finally enriched by Zn substitution or sulfide precipitation. | ZHU Guocai ZHENG Mingdan FAN Guanglong ZHAO Yuna | 2007 | Rare Metals2007，26，5： | 12 |
| 5 | Structural basis for the impact of phosphorylation on the activation of plant receptor-like kinase BAK1显示文摘 | Liming Yan Yuanyuan Ma Dan Liu Xiaochao Wei Yuna Sun Xiaoyue Chen Huadong Zhao Jingwen Zhou Zhiyong Wang Wenqing Shui Zhiyong Lou | 2012 | Cell Research2012，22，8： | 10 |
| 6 | Risk factors associated with emergency peripartum hysterectomy显示文摘 | JinRong Guo Yuna Chen Yan | 2014 | Chinese Medical Journal2014，，5： | 10 |
| 7 | Corrosion Behavior of Extruded near Eutectic Al—Si—Mg and 6063 Alloys显示文摘In this work,a comparison study on corrosion behavior of extruded near eutectic Al—12.3%Si—0.26%Mg and 6063 alloys has been carried out by mass loss test in 4%H2SO4 aqueous solution in the open air and potentiodynamic polarization test in 3.5 wt.%NaCI aqueous solution.Results indicate that the corrosion resistance of the near eutectic Al—Si—Mg alloy is less than that of 6063 alloy.Macro/microscopy and scanning electron microscopy results clearly show the difference of the corrosion progress of these two alloys in 4%H2SO4 aqueous solution.The corrosion type of 6063 alloy is pitting corrosion.The Mg2Si and AlFeSi particles and surface defects act as nucleation sites for pitting,and the amount and distribution of them have a significant effect on the pitting behavior.For the near eutectic alloy,there are two types of corrosion cells. One is between the extruded primaryα-AI and the eutectic,the other is between the eutectic Al and eutectic Si particles.Combination of these two types of corrosion cells leads to a lower corrosion resistance,a higher mass loss of the near eutectic alloy compared with 6063 alloy,and the formation of the paralleling corroded grooves. | Yuna Wu Hengcheng Liao | 2013 | Journal of Materials Science & Technology2013，29，4： | 8 |
| 8 | Structure Analysis and Function Evaluation of a Kind of Fuzzy PID Controllers显示文摘In this paper, a kind of fuzzy PID (Proportional integral and derivate) controllers is discussed, which has 3 input variables (error, difference of error, sum of error) and one output variable; triangular fully-overlapped symmetric membership function for input variables; singleton equally-spaced membership function for the output variable; linear control rules; Sum-Product inference method; and Center of area (COA) defuzzification algorithm. The paper consists of three main parts. In the first part, the structure properties of fuzzy PID controllers are studied. The explicit expression of this kind of fuzzy PID controllers is derived. It is proved that the analytical structure of fuzzy PID controllers is the sum of a global three-dimensional multi-level relay and a local nonlinear controller. When the number of fuzzy sets tends to infinity, the local nonlinear controller will disappear, and the degree of nonlinearity of the fuzzy PID controller becomes zero. In the second part, the function properties of fuzzy PID controllers are studied. It is revealed that the fuzzy PID controller is a variable gain nonlinear PID controller; so linear PID controllers can be regarded as a special example of fuzzy PID controllers. Moreover, they are equivalent to the sum of three fuzzy controllers with one-to-one mapping; so they do not suffer from some weaknesses such as composed-action, input coupling, etc. Based on these theoretical results, a systematic design approach of fuzzy PID control systems is proposed and demonstrated by 2 simulation examples in the third part of this paper. It is shown that the proposed fuzzy PID controller not only has good structure and function characteristics, but also can be very simply and quickly designed; therefore, it is very suitable for a wide range of applications. | DUXinyu ZHANGNaiyao YUNa | 2004 | Chinese Journal of Electronics2004，13，4： | 6 |
| 9 | Mussel-inspired agarose hydrogel scaffolds for skin tissue engineering显示文摘Polysaccharide hydrogels are widely used in tissue engineering because of their superior biocompatibility and low immunogenicity.However,many of these hydrogels are unrealistic for practical applications as the cost of raw materials is high,and the fabrication steps are tedious.This study focuses on the facile fabrication and optimization of agarose-polydopamine hydrogel(APG)scaffolds for skin wound healing.The first study objective was to evaluate the effects of polydopamine(PDA)on the mechanical properties,water holding capacity and cell adhesiveness of APG.We observed that APG showed decreased rigidity and increased water content with the addition of PDA.Most importantly,decreased rigidity translated into significant increase in cell adhesiveness.Next,the slow biodegradability and high biocompatibility of APG with the highest PDA content(APG3)was confirmed.In addition,APG3 promoted full-thickness skin defect healing by accelerating collagen deposition and promoting angiogenesis.Altogether,we have developed a straightforward and efficient strategy to construct functional APG scaffold for skin tissue engineering,which has translation potentials in clinical practice. | Ting Su Mengying Zhang Qiankun Zeng Wenhao Pan Yijing Huang Yuna Qian Wei Dong Xiaoliang Qi Jianliang Shen | 2021 | Bioactive Materials2021，6，3： | 6 |
| 10 | 酶解乳蛋白对初生仔猪小肠组织学结构及隐窝细胞增殖的影响(英文)显示文摘INTRODUCTIONTheintestineasthemajorinterfacebetweenanorganismanditsnutritionalenvironmentplaysacriticalroleinpostnataldevelopm... | Tian Wang 1,Ruo Jun Xu 2, Yuna Qiang Zhang 3, Chong Tian Zheng 1 and Jian Cheng Zhang 4 1 Department of Animal Nutrition and Feed, Nanjing Agricultural University,210095 2 Department of Zoology, The University of Hong Kong 3 Department | 1999 | 中国组织化学与细胞化学杂志1999，8，2： | 5 |
| 11 | Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharideinduced inflammatory responses in broilers显示文摘Background:Lactobacillus reuteri strains are widely used as probiotics to prevent and treat inflammatory bowel disease by modulating the host’s immune system.However,the underlying mechanisms by which they communicate with the host have not been clearly understood.Bacterial extracellular vesicles(EVs)have been considered as important mediators of host-pathogen interactions,but their potential role in commensals-host crosstalk has not been widely studied.Here,we investigated the regulatory actions of EVs produced by L.reuteri BBC3,a gut-associated commensal bacterium of Black-Bone chicken,in the development of lipopolysaccharide(LPS)-induced intestinal inflammation in a chicken model using both in vivo and in vitro experiments.Results:L.reuteri BBC3 produced nano-scale membrane vesicles with the size range of 60-250 nm.Biochemical and proteomic analyses showed that L.reuteri BBC3-derived EVs(LrEVs)carried DNA,RNA and several bioactive proteins previously described as mediators of other probiotics’beneficial effects such as glucosyltransferase,serine protease and elongation factor Tu.In vivo broiler experiments showed that administration of LrEVs exerted similar effects as L.reuteri BBC3 in attenuating LPS-induced inflammation by improving growth performance,reducing mortality and decreasing intestinal injury.LrEVs suppressed the LPS-induced expression of pro-inflammatory genes(TNF-α,IL-1β,IL-6,IL-17 and IL-8),and improved the expression of anti-inflammatory genes(IL-10 and TGF-β)in the jejunum.LrEVs could be internalized by chicken macrophages.In vitro pretreatment with LrEVs reduced the gene expression of TNF-α,IL-1βand IL-6 by suppressing the NF-κB activity,and enhanced the gene expression of IL-10 and TGF-βin LPS-activated chicken macrophages.Additionally,LrEVs could inhibit Th1-and Th17-mediated inflammatory responses and enhance the immunoregulatory cells-mediated immunosuppression in splenic lymphocytes of LPS-challenged chickens through the activation of macrophages.Finally,we revealed that the reduced content of both vesicular proteins and nucleic acids attenuated the suppression of LrEVs on LPS-induced inflammatory responses in ex vivo experiments,suggesting that they are essential for the LrEVs-mediated immunoregulation.Conclusions:We revealed that LrEVs participated in maintaining intestinal immune homeostasis against LPSinduced inflammatory responses in a chicken model.Our findings provide mechanistic insight into how commensal and probiotic Lactobacillus species modulate the host’s immune system in pathogens-induced inflammation. | Rujiu Hu Hua Lin Mimi Wang Yuezhen Zhao Haojing Liu Yuna Min Xiaojun Yang Yupeng Gao Mingming Yang | 2021 | Journal of Animal Science and Biotechnology2021，12，3： | 5 |
| 12 | The nucleoprotein of severe fever with thrombocytopenia syndrome virus processes a stable hexameric ring to facilitate RNA encapsidation显示文摘Severe fever with thrombocytopenia syndrome virus(SFTSV),a member of the Phlebovirus genus from the Bunyaviridae family endemic to China,is the causative agent of life-threatening severe fever with thrombocyto-penia syndrome(SFTS),which features high fever and hemorrhage.Similar to other negative-sense RNA viruses,SFTSV encodes a nucleocapsid protein(NP)that is essen-tial for viral replication.NP facilitates viral RNA encapsida-tion and is responsible for the formation of ribonucleopro-tein complex.However,recent studies have indicated that NP from Phlebovirus members behaves in inhomogene-ous oligomerization states.In the present study,we report the crystal structure of SFTSV NP at 2.8Åresolution and demonstrate the mechanism by which it processes a ring-shaped hexameric form to accomplish RNA encapsida-tion.Key residues essential for oligomerization are identi-fi ed through mutational analysis and identifi ed to have a signifi cant impact on RNA binding,which suggests that correct formation of highly ordered oligomers is a criti-cal step in RNA encapsidation.The fi ndings of this work provide new insights into the discovery of new antiviral reagents for Phlebovirus infection. | Honggang Zhou Yuna Sun Ying Wang Min Liu Chao Liu Wenming Wang Xiang Liu Le Li Fei Deng Hualin Wang Yu Guo Zhiyong Lou | 2013 | Protein & Cell2013，4，6： | 4 |
| 13 | Synthesis,structure and luminescence properties of metal-organic frameworks based on benzo-bis(imidazole)显示文摘Metal-organic frameworks(MOFs)constructed from conjugated organic ligands are candidates for hybrid photoactive materials with potential applications.Compared to that from the ligands only,the intensity and wavelength of the luminescence could be tuned after they were incorporated in extended framework.In this report,by using an organic ligand with azolate moiety,benzo-bis(imidazole)(H2BBI),we synthesized two new MOF structures.Framework 1([Co(H2BBI)(DMSO)2Cl2]n,DMSO=dimethyl sulfoxide),constructed from tetrahedral Co(II)and H2BBI,exhibits zigzag 1D structure.Meanwhile,framework 2([Cu2(H2BBI)3(DMSO)6(NO3)4]n),a layered structure with hcb topology,was assembled from tetragonal pyramidal Cu(II)and H2BBI.Furthermore,2 exhibits strong luminescence emission(ex=280 nm).A blue shift of 40 nm(from 359 nm to 319 nm)was observed in framework 2 compared to the free ligand,which could be explained by the ligand-to-metal charge transfer in the network. | YIN XinBo SONG YuNa WANG Yang ZHANG LiJuan LI QiaoWei | 2014 | Science China Chemistry2014，57，1： | 4 |
| 14 | Effect of Si Content on Dynamic Recrystallization of Al-Si-Mg Alloys During Hot Extrusion显示文摘By using electron backscatter diffraction(EBSD) and transmission electron microscopy(TEM), the effect of Si content on microstructure characteristics of three as-extruded Ale SieM g alloys was investigated. Results showed that the density of coarse Si particles played a critical role in dynamic recrystallization. Dynamic recrystallization rarely occurred in S1 alloy with less Si content; however, it happened in the Si-rich zones in S2 alloy with a medium Si content. And a mature recrystallization was observed in S3 alloy with high Si content. Although deformation was carried out at high temperature, particle-stimulated dynamic recrystallization occurred in Si-rich zones. | Yuna Wu Hengcheng Liao Jian Yang Kexin Zhou | 2014 | Journal of Materials Science & Technology2014，30，12： | 4 |
| 15 | A versatile building block:the structures and functions of negative-sense single-stranded RNA virus nucleocapsid proteins显示文摘Nucleocapsid protein(NPs)of negative-sense single-stranded RNA(-ssRNA)viruses function in different stages of viral replication,transcription,and maturation.Structural investigations show that-ssRNA viruses that encode NPs preliminarily serve as structural building blocks that encapsidate and protect the viral genomic RNA and mediate the interaction between genomic RNA and RNA-dependent RNA polymerase.However,recent structural results have revealed other bio-logical functions of-ssRNA viruses that extend our understanding of the versatile roles of virally encoded NPs. | Yuna Sun Yu Guo Zhiyong Lou | 2012 | Protein & Cell2012，3，12： | 3 |
| 16 | Reduction of Low-grade Manganese Dioxide Ore Pellets by Biomass Wheat Stalk显示文摘An investigation on reducing low-grade manganese dioxide ore pellets was carried out by using wheat stalk as reductant. The main impact factors of reduction percent such as particle size, biomass/ore ratio, heating rate, nitrogen flow rate, temperature and time in reduction process were discussed. The morphology and component of manganese dioxide ore particle at different stages were also analyzed by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS). The results show the increase of the reduction temperature and time, biomass/ore ratio, and the decrease of particle size, heating rate and nitrogen flow rate can significantly enhance reduction efficiency. The reduction kinetic of the manganese ore is controlled by three-dimensional mass diffusion of gaseous reductive volatiles passing from the surface to the core of the ore particles. The activation energy E and frequency factor A were calculated to be 24.15 kJ · mol-1 and 166 min-1 , respectively. Biomass pyrolysis volatiles adsorbed onto the surface of the ore particle leads to serious variation of the microstructures and chemical composition of the manganese ore particles. | Honglei ZHANG Guocai ZHU Hong YAN Yuna ZHAO Tiancheng LI Xiujuan FENG | 2013 | Acta Metallurgica Sinica(English Letters)2013，26，2： | 3 |
| 17 | Osteopontin is a biomarker for early autoimmune uveoretinitis显示文摘Osteopontin(OPN)is an extracellular matrix protein with a diverse range of functions,including roles in cell adhesion,migration,and immunomodulation,which are associated with the modulation of neuroinflammation in the central nervous system.The present study was performed to evaluate the involvement of OPN in the eyes of an experimental autoimmune uveoretinitis(EAU)model.The EAU model was developed by immunization of Lewis rats with interphotoreceptor retinoid-binding protein.The results showed the OPN level was remarkably upregulated in the eye of EAU rats on day 9 post-immunization.The level of CD44,a ligand of OPN,was increased in the ciliary body of EAU rats.Furthermore,OPN was also detected in the ciliary body and activated microglia/macrophages in the EAU retina.The results suggest that OPN was significantly upregulated in the eyes of EAU rats,and that it may be useful as an early biomarker of ocular autoimmune diseases.All animal experiments were approved by the Institutional Animal Care and Use Committee of Jeju National University(approval No.2020-0012)on March 11,2020. | Jeongtae Kim Meejung Ahn Yuna Choi Jiyoon Chun Kyungsook Jung Akane Tanaka Hiroshi Matsuda Taekyun Shin | 2022 | Neural Regeneration Research2022，17，7： | 3 |
| 18 | Kinetics of recovering germanium from lignite ash with chlorinating roasting methods显示文摘由使氯化烤恢复 Ge 的一个过程被提出。因为它的低沸腾点, GeCl4 与褐煤灰被分开并且恢复。运动分析显示使氯化烤过程与缩小模型的 unreacted 核心适合,反应率方程对应于 1 吗?2a/3?( 1?一) 2/3 = kt。明显的激活精力 Ea 被计算是在 pH = 的媒介的 22.36 kJ D301R 树脂 3.47。静态地浸透的吸附能力是 410 mg/g。在 D301R 树脂上吸附的磅(IV ) 能是由 1.0-2.0 mol/L NaOH 的 eluted。率常数是 k298 = 5. | ZHU Guocai WANG Jingyan CHENG Zhuo ZHAO Yuna | 2008 | Rare Metals2008，27，3： | 3 |
| 19 | Polydopamine nanoparticle-dotted food gum hydrogel with excellent antibacterial activity and rapid shape adaptability for accelerated bacteria-infected wound healing显示文摘Most commonly used wound dressings have severe problems,such as an inability to adapt to wound shape or a lack of antibacterial capacity,affecting their ability to meet the requirements of clinical applications.Here,a nanocomposite hydrogel(XKP)is developed by introducing polydopamine nanoparticles(PDA NPs)into a food gum matrix(XK,consisting of xanthan gum and konjac glucomannan,both FDA-approved food thickening agents)for skin wound healing.In this system,the embedded PDA NPs not only interact with the food gum matrix to form a hydrogel with excellent mechanical strength,but also act as photothermal transduction agents to convert near-infrared laser radiation to heat,thereby triggering bacterial death.Moreover,the XKP hydrogel has high elasticity and tunable water content,enabling it to adapt to the shape of the wound and insulate it,providing a moist environment suitable for healing.In-vivo skin wound healing results clearly demonstrate that XKP can significantly accelerate the healing of wounds by reducing the inflammatory response and promoting vascular reconstruction.In summary,this strategy provides a simple and practical method to overcome the drawbacks of traditional wound dressings,and provides further options when choosing suitable wound healing materials for clinical applications. | Qiankun Zeng Yuna Qian Yijing Huang Feng Ding Xiaoliang Qi Jianliang Shen | 2021 | Bioactive Materials2021，6，9： | 3 |
| 20 | Genome sequencing and phylogenetic analysis of allotetraploid Salix matsudana Koidz显示文摘Polyploidy is a common phenomenon among willow species.In this study,genome sequencing was conducted for Salix matsudana Koidz(also named Chinese willow),an important greening and arbor tree species,and the genome of this species was compared with those of four other tree species in Salicaceae.The total genome sequence of S.matsudana was 655.72 Mb in size,with repeated sequences accounting for 45.97%of the total length.In total,531.43 Mb of the genome sequence could be mapped onto 38 chromosomes using the published genetic map as a reference.The genome of S.matsudana could be divided into two groups,the A and B genomes,through homology analysis with the genome of Populus trichocarpa,and the A and B genomes contained 23,985 and 25,107 genes,respectively.4DTv combined transposon analysis predicted that allotetraploidy in S.matsudana appeared~4 million years ago.The results from this study will help reveal the evolutionary history of S.matsudana and lay a genetic basis for its breeding. | Jian Zhang Huwei Yuan Yujuan Li Yanhong Chen Guoyuan Liu Meixia Ye Chunmei Yu Bolin Lian Fei Zhong Yuna Jiang Jichen Xu | 2020 | Horticulture Research2020，7，1： | 3 |
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