| 1 | Identification of an epitope of SARS-coronavirus nucleocapsid protein显示文摘The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a majorvirion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV werepredicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodieswere isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV. | YINGLIN XuSHEN RUIFuYANG YIXUELI YONGYONGJI YouYuHE MuDESHI WEILU TIELIUSHI BINGSUN JINWANG HONGXIAWANG HUALIANGJIANG JIANHUASHEN YOUHUAXIE YUANWANG GANGPEI BBIFENSHEN JIARUIWU | 2003 | Cell Research2003，13，3： | 22 |
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