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| 1 | Identification of an epitope of SARS-coronavirus nucleocapsid protein显示文摘The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a majorvirion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV werepredicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodieswere isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV. | YINGLIN XuSHEN RUIFuYANG YIXUELI YONGYONGJI YouYuHE MuDESHI WEILU TIELIUSHI BINGSUN JINWANG HONGXIAWANG HUALIANGJIANG JIANHUASHEN YOUHUAXIE YUANWANG GANGPEI BBIFENSHEN JIARUIWU | 2003 | Cell Research2003，13，3： | 22 |
| 2 | Differential regulation of survivin by p53 contributes to cell cycle dependent apoptosis显示文摘Recent studies indicate that cell-cycle checkpoints are tightly correlated with the regulation of apoptosis, in which p53 plays an important role. Our present works show that the expression of E6/E7 oncogenes of human papillomavirus in HeLa cells is inhibited in the presence of anti-tumor reagent tripchlorolide (TC), which results in the up-regulation of p53 in HeLa cells. Interestingly, under the same TC-treatment, the cells at the early S-phase are more susceptible to apoptosis than those at the middle S-phase although p53 protein is stabilized to the same level in both situations. Significant difference is exhibited between the two specified expression profiles. Further analysis demonstrates that anti-apoptotic gene survivin is up-regulated by p53 in the TC-treated middle-S cells, whereas it is down-regulated by p53 in the TC-treated early-S cells. Taken together, the present study indicates that the differential p53-regulated expres- sion of survivin at different stages of the cell cycle results in different cellular outputs under the same apoptosis-inducer. | YanJIN YongWEI LeiXIONG YingYANG JiaRuiWU | 2005 | Cell Research2005，15，5： | 21 |
| 3 | Induction of mitochondrion-mediated apoptosis of CHO cells by tripchloro lide显示文摘Tripchlorolide (TC) is a potent antitumor reagent purified from a Chinese herb Tripterygium Wilfordii Hook. f.. However, its cellular effects and mechanism of action are unknown. We showed here that TC induced apoptosis of Chinese Hamster Ovary (CHO) cells in time- and dose-dependent manners. TC resulted in the degradation of Bcl-2, the translocation of Bax from the cytosol to mitochondria, and the release of cytochrome c from mitochondria. Stable overexpression of human Bcl-2 could reduce the apoptosis of TCtreated cells by blocking the translocation of Bax and the release of cytochrome c. These results indicate that TC induces apoptosis of CHO cell by activating the mitochondrion-mediated apoptotic pathway involving the proteins of Bcl-2 family and cytochrome c. | YANREN LEIXIONG JIARUIWU | 2003 | Cell Research2003，13，4： | 11 |
| 4 | Qingkailing injection for the treatment of acute stroke: a systematic review and Meta-analysis显示文摘OBJECTIVE: To evaluate systematically the clinical efficacy and safety of Qingkailing(QKL) injection in the treatment of acute stroke.METHODS: Searches for randomized controlled trials into acute stroke treated with QKL injection were performed in the China National Knowledge Infrastructure Database, China Science and Technology Journal Database, Wan fang Database,Chinese Biomedical Literature Database, PubMed and Cochrane Library, from January 1979 to March2013. Two reviewers independently retrieved the RCTs and extracted the information. The Cochrane risk of bias method was used to assess the quality of the included studies, and a Meta-analysis was conducted with Review Manager 5.2 software.RESULTS: A total of 13 studies with 1110participants were included. The quality of the studies was generally low. The Meta-analysis indicated that the combined use of QKL and Western Medicine was significantly superior to control group therapy in terms of the total effective rate. The relative risk(RR) in the acute cerebral hemorrhage(ACH) sub-group was 1.17 [95%confidence interval(CI)(1.08, 1.26), P=0.0001]. In the acute cerebral infarction(ACI) sub-group, RR was 1.27 [95% CI(1.14, 1.42), P<0.0001], and in the ACH and ACI mixed sub-group, RR was 1.34 [95% CI(1.20,1.50),P<0.00001]. Additionally, QKL promoted the absorption of hematoma [mean difference(MD)=﹣3.73, 95%CI(﹣4.48, ﹣2.98), P<0.000 01],decreased neurological damage in ACI [MD=﹣5.60, 95% CI(﹣8.50, ﹣2.70), P=0.0002] and ACH[MD= ﹣ 4.08, 95% CI( ﹣ 8.00, ﹣ 0.16), P=0.04],promoted the recovery of awareness [RR=1.56, 95%CI(1.09, 2.21), P=0.01] and reduced the whole blood viscosity coefficient [MD=﹣ 0.75, 95% CI( ﹣1.47, ﹣0.03), P=0.04]. There were no adverse drug reactions reported in the included studies.CONCLUSION: Based on this systematic review,QKL combined with conventional therapy was effective compared with control treatment.However, because the articles used in the study were not of high quality, further studies should be conducted into the efficacy and safety of QKL in treating acute stroke. | JiaruiWu Xiaomeng Zhang Bing Zhang | 2014 | Journal of Traditional Chinese Medicine2014，34，2： | 9 |
| 5 | Differential activation of intra-S-phase checkpoint in response to tripchlorolide and its effects on DNA replication显示文摘DNA replication is tightly regulated during the S phase of the cell cycle, and the activation of the intra-S-phase checkpoint due to DNA damage usually results in arrest of DNA synthesis. However, the molecular details about the correlation between the checkpoint and regulation of DNA replication are still unclear. To investigate the connections between DNA replication and DNA damage checkpoint, a DNA-damage reagent, tripchlorolide, was applied to CHO (Chinese ovary hamster) cells at early- or middle-stages of the S phase. The early-S-phase treatment with TC significantly delayed the progression of the S phase and caused the phosphorylation of the Chk1 checkpoint protein, whereas the middle-S-phase treatment only slightly slowed down the progression of the S phase. Furthermore, the analysis of DNA replication patterns revealed that replication pattern Ⅱ was greatly prolonged in the cells treated with the drug during the early-S phase, whereas the late-replication patterns of these cells were hardly detected, suggesting that the activation of the intra-S-phase checkpoint inhibits the late-origin firing of DNA replication. We conclude that cells at different stages of the S phase are differentially sensitive to the DNA-damage reagent, and the activation of the intra-Sphase checkpoint blocks the DNA replication progression in the late stage of S phase. | YanREN JiaRuiWU | 2004 | Cell Research2004，14，3： | 2 |
| 6 | Insights into complex processes of animal development显示文摘 | JiaruiWu | 2014 | Journal of Molecular Cell Biology2014，8，4： | 0 |
| 7 | Research on Operation Optimization of Heating System Based on Electric Storage Coupled Solar Energy and Air Source Heat Pump显示文摘For heating systems based on electricity storage coupled with solar energy and an air source heat pump(ECSA),choosing the appropriate combination of heat sources according to local conditions is the key to improving economic efficiency.In this paper,four cities in three climatic regions in China were selected,namely Nanjing in the hot summer and cold winter region,Tianjin in the cold region,Shenyang and Harbin in the severe cold winter region.The levelized cost of heat(LCOH)was used as the economic evaluation index,and the energy consumption and emissions of different pollutants were analyzed.TRNSYS software was used to simulate and analyze the system performance.The Hooke-Jeeves optimization algorithm and GenOpt software were used to optimize the system parameters.The results showed that ECSA systemhad an excellent operation effect in cold region and hot summer and cold winter region.Compared with ECS system,the systemenergy consumption,and the emission of different pollutants of ECSA system can be reduced by a maximum of 1.37 times.In cold region,the initial investment in an air source heat pump is higher due to the lower ambient temperature,resulting in an increase in the LOCH value of ECSA system.After the LOCH value of ECSA system in each region was optimized,the heating cost of the system was reduced,but also resulted in an increase in energy consumption and the emission of different pollutant gases. | Jingxiao Han Chuanzhao Zhang LuWang ZengjunChang Qing Zhao Ying Shi JiaruiWu Xiangfei Kong | 2023 | Energy Engineering2023，120，9： | 0 |
| 8 | 31nstitute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Taiyuan Road,Shanghai 200031, China.显示文摘 Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC43, AIBV and BcoV, are proteolytically cleaved into two subunits, S 1 and S2. In contrast, TGV, FIPV and HCoV-229E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S 1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S 1 and S2 Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells. | XiaoDongWU BoSHANG RuiFuYANG HaoYU ZhiHalMA XuSHEN YongYongJI YingLIN YaDiWU GuoMeiLIN LinTIAN XiaoQingGAN ShengYANG WeiHongJIANG ErHeiDAI XiaoYiWANG HuaLiangJIANG YouHuaXIE XueLiangZHU GangPEI LinLI JiaRuiWU BingSUN | 2004 | Cell Research2004，14，5： | 0 |
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