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| 1 | Identification of an epitope of SARS-coronavirus nucleocapsid protein显示文摘The nucleocapsid (N) protein of severe acute respiratory syndrome-coronavirus (SARS-CoV) is a majorvirion structural protein. In this study, two epitopes (N1 and N2) of the N protein of SARS-CoV werepredicted by bioinformatics analysis. After immunization with two peptides, the peptides-specific antibodieswere isolated from the immunized rabbits. The further experiments demonstrated that N1 peptide-inducedpolyclonal antibodies had a high affinity to bind to E. coli expressed N protein of SARS-CoV. Furthermore, itwas confirmed that N1 peptide-specific IgG antibodies were detectable in the sera of severe acute respiratorysyndrome (SARS) patients. The results indicated that an epitope of the N protein has been identified andN protein specific Abs were produced by peptide immunization, which will be useful for the study of SARS-CoV. | YINGLIN XuSHEN RUIFuYANG YIXUELI YONGYONGJI YouYuHE MuDESHI WEILU TIELIUSHI BINGSUN JINWANG HONGXIAWANG HUALIANGJIANG JIANHUASHEN YOUHUAXIE YUANWANG GANGPEI BBIFENSHEN JIARUIWU | 2003 | Cell Research2003，13，3： | 22 |
| 2 | RPE Query Processing and Optimization Techniques for XML Databases显示文摘An extent join to compute path expressions containing parent-children and ancestor-descendent operations and two path expression optimization rules, path-shortening and path-complementing, are presented in this paper. Path-shortening reduces the number of joins by shortening the path while path-complementing optimizes the path execution by using an equivalent complementary path expression to compute the original one.Experimental results show that the algorithms proposed are more efficient than traditional algorithms. | Guo-RenWang BingSun Jian-HuaLv GeYu | 2004 | Journal of Computer Science & Technology2004，19，2： | 7 |
| 3 | Expression of Intracellular Domain of Epidermal Growth Factor Receptor and Generation of Its Monoclonal Antibody显示文摘To prepare monoclonal antibody specific to epidermal growth factor receptor (EGFR) intracellular domain, its gene was amplified from total RNA of A431 cell by RT-PCR. Then the gene was cloned into prokaryotic vector pET30a(+). The recombinant plasmid was transformed into E. Coli BL21 (DE3) strain for protein expression.Recombinant protein was induced with IPTG and purified using Ni2+-NTA agarose. Then the anti-EGFR monoclonal antibody (nAb) was prepared with classical hybridoma technique. Positive clones were selected using indirect enzyme-linked inmunoabsorbent assay (ELISA). Totally 4 hybridoma clones were obtained and these mAbs were IgG1 (3 clones) and IgG2a (1 clone), respectively. Their light chains were all kappa chains.Western blotting analysis and confocal immunofluorescence assays demonstrated that mAbs could specifically recognize EGFR expressing on A431 carcinoma cell line. The mAbs will be useful in the study of EGFR-mediated signal transduction. | YingLin ZhiduoLiu JianminJiang ZiqingJiang Yongyongji BingSun | 2004 | Cellular & Molecular Immunology2004，1，2： | 2 |
| 4 | PASSIVE-ACTIVE CONTROL OF A FLEXIBLE ISOLATI0N SYSTEM显示文摘Passive-active control of a flexible isolation system is investigated from the viewpoint of power flow. Dynamic transfer equations of the system are deduced based on a matrix method which uses mobility or impedance representations of three substructures: the source of vibration, the receiver and the mounting system which connects the source to the receiver. The cancellation of axial input forces to the receiver is considered as the active control strategy and its effects are discussed. The results of the study show that the strategy adopted herein can effectively reduce the power transmitted to the receiver. | Song KongjieZhang BingSun LinglingSun YuguoSchool of Mechanical Engineering, Shandong University, Jinan 250061, China | 2003 | Chinese Journal of Mechanical Engineering2003，16，4： | 0 |
| 5 | XML数据库系统中RPE查询处理与优化技术显示文摘随着Internet上应用技术的快速发展,Web上有大量的信息可以利用,并且几乎所有的文档都是半结构化的。XML正在成为Web信息表示与数据交换的标准,并被越来越多的应用于信息描述。尽管XML通常用作信息交换标准,但如何存储、索引和查询XML数据仍然是一个非常重要的研究问题,并且已经成为学术界和工业界的热点研究问题。到目前为止,人们对XML数据管理技术的各种问题进行了非常深入地研究工作。本文的主要贡献如下: | Guo-RenWang BingSun Jian-HuaLv GeYu | 2004 | Journal of Computer Science & Technology2004，19，C00： | 0 |
| 6 | 31nstitute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 320 Taiyuan Road,Shanghai 200031, China.显示文摘 Spike protein is one of the major structural proteins of severe acute respiratory syndrome-coronavirus. It is essential for the interaction of the virons with host cell receptors and subsequent fusion of the viral envelop with host cell membrane to allow infection. Some spike proteins of coronavirus, such as MHV, HCoV-OC43, AIBV and BcoV, are proteolytically cleaved into two subunits, S 1 and S2. In contrast, TGV, FIPV and HCoV-229E are not. Many studies have shown that the cleavage of spike protein seriously affects its function. In order to investigate the maturation and proteolytic processing of the S protein of SARS CoV, we generated S 1 and S2 subunit specific antibodies (Abs) as well as N, E and 3CL protein-specific Abs. Our results showed that the antibodies could efficiently and specifically bind to their corresponding proteins from E.coli expressed or lysate of SARS-CoV infected Vero-E6 cells by Western blot analysis. Furthermore, the anti-S 1 and S2 Abs were proved to be capable of binding to SARS CoV under electron microscope observation. When S2 Ab was used to perform immune precipitation with lysate of SARS-CoV infected cells, a cleaved S2 fragment was detected with S2-specific mAb by Western blot analysis. The data demonstrated that the cleavage of S protein was observed in the lysate, indicating that proteolytic processing of S protein is present in host cells. | XiaoDongWU BoSHANG RuiFuYANG HaoYU ZhiHalMA XuSHEN YongYongJI YingLIN YaDiWU GuoMeiLIN LinTIAN XiaoQingGAN ShengYANG WeiHongJIANG ErHeiDAI XiaoYiWANG HuaLiangJIANG YouHuaXIE XueLiangZHU GangPEI LinLI JiaRuiWU BingSUN | 2004 | Cell Research2004，14，5： | 0 |
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