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| 1 | FADD and TRADD expression and apoptosis in primary hepatocellular carcinoma显示文摘AIM To investigate the clinical features ofFADD and TRADD expressions in primaryhepatocellular carcinoma(HCC)and todetermine their relationship with hepaticapoptosis.METHODS FADD and TRADD expressions weredetected by immunohistochemistry and hepaticapoptosis were determined by in situ end-labeling(ISEL).RESULTS Ten(25.6%)cases of HCC weredetected to express FADD protein.The positiverate in HCC is lower than that in non-cancerousadjacent liver tissues(62.5%)(P<0.05).Inthose of grade Ⅰ-Ⅱ,8(38.1%)cases wereFADD positive,while only 2/18(11.1%)casesof grade Ⅲ-Ⅳ had detectable FADD protein(P<0.05).No relationship was found betweenFADD expression and other clinical features,such as gender,age,tumor size,differentiationor metastasis.ISEL positive cells can be seen inall cases of HCC.The hepatic apoptosis wasassociated with FADD expression as moreapoptotic cells were detected in those caseswhich had moderately to strongly positiveFADD,as compared with negative or weakpositive FADD cases(P<0.05).No relationshipwas found between FADD expression and hepaticapoptosis in non-cancerous adjacent livertissues.Fifteen of 39(38.5%)cases of HCCwere found positive for TRADD protein,andsimilar positive rate(37.5%)in non-cancerousadjacent liver tissues(P>0.05).The expressionof TRADD is correlated with HCC differentiation, as only 22.2% of moderately to highlydifferentiated HCC showed positive TRADDprotein,while as high as 52.4% of poorlydifferentiated HCC had TRADD(P<0.05),Norelationship was found between TRADDexpression and gender,age,tumor size or gradeor metastasis,although 42.9% of HCC of gradeⅠ/Ⅱ showed positive TRADD which wasslightly higher than that of grade Ⅲ/Ⅳ(33.3%,P>0.05).Hepatic apoptosis was not related toTRADD expression in HCC or non-cancerousadjacent liver tissues.CONCLUSION Loss of FADD expression playsan important role in HCC carcinogenesis,andexpression of TRADD also contributes to HCCdevelopment.The cell apoptosis in HCC isassociated with FADD expression.However,theexpression of TRADD does not correlate wellwith hepatic apoptosis in HCC. | Sun BH Zhao XP Wang BJ Yang DL Hao LJ | 2000 | World Journal of Gastroenterology2000，6，2： | 22 |
| 2 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 3 | Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver显示文摘AIM To improve the Cultivation efficiency and yield of human liver cell line CL--l.METHODS High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and proliferation.The specific functions of human liver cell werealso determined.RESULTS Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional cultivation.The amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, respectively.CONCLUSION The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver. | Gao Y Xu XP Hu HZ Yang JZ | 1999 | World Journal of Gastroenterology1999，5，3： | 13 |
| 4 | Apoptosis of neoplasm cell lines induced byhepatic peptides extracted from sucking porcine hepatocytes显示文摘 | Kong XP Zou QY Li RB Zheng PL Yang LP Jin SW | 1999 | World Journal of Gastroenterology1999，5，5： | 11 |
| 5 | Effect of ACE inhibitors and angiotensin II type 1 receptor antagonists on endothelial NO synthase knockout mice with heart failure显示文摘 | Liu YH Xu J Yang XP | 2002 | Hypertension2002，39，22： | 2 |
| 6 | Increased atherosclerotic lesions in apoE mice with plasma phospholipid transfer pro- tein overexpression 显示文摘 | Yang XP Yan D Qiao C | 2003 | Arterioscler Thromb Vasc Biol2003，23，9： | 1 |
| 7 | Fractalkine upregulatesintercellular adhesion molecule-1 in endothelial cells throughCX3CR1 and the Jak Stat5 pathway 显示文摘 | Yang XP Mattagajasingh S Su S | 2007 | Circ Res2007，101，10： | 1 |
| 8 | Opposing regulation of the locus encoding IL-17 through diroet, reciprocal actions of STAT3 and STATS显示文摘 | Yang XP C horesehi K Steward-11ua-p SM | 2011 | Nat Immunol2011，12，3： | 1 |
| 9 | The functional alpha-adrenoceptor in dog caudal vesical arteries is mainly an alpha1A subtype显示文摘 | Nakazawa M Taguchi Y Yang XP | 2002 | Auton-Autacoid-Pharmacol2002，22，56： | 1 |
| 10 | The role of IL-15 in activating STATS and fine-tuning IL:17A production in CIM T lymphocytes显示文摘 | Pandiyan P Yang XP Saravanamuthu SS et 81 | 2012 | J lmmunol2012，189，9： | 1 |
| 11 | The prognostic role of the class Ⅲ beta-tubulin in non-small cell lung cancer (NSCLC) pa- tients receiving the taxane/vinorebine-based chemotherapy: a meta- analysis 显示文摘 | Yang YL Luo XP Xian L | 2014 | PLoS One2014，9，93： | 1 |
| 12 | Signal transducer and activator of transcription 3alpha and specificity protein 1 interact to upregulate intercellular adhesion molecule-1 in ischemic-reperfused myocardium and vascular endothelium显示文摘 | Yang XP Irani K Mattagajasingh S | 2005 | Arterioscler Thromb Vasc Biol2005，25，： | 1 |
| 13 | Interleukin-27 priming of T cells controls IL-17 production in trans via induction of the ligand PD-L1显示文摘 | Hirahara K Ghoreschi K Yang XP | | 0，，06： | 1 |
| 14 | Structural insight into the DNA polymerase beta deoxyribose phosphate lyase mechanism显示文摘 | Prasad R Batra VK Yang XP | 2005 | DNA Repair2005，4，12： | 1 |
| 15 | Hepatocyte growth factor/scatter factor differentially regulates expression of proangiogenic factors throush Egr-1 in head and neck squamous cell carcinoma显示文摘 | Worden B Yang XP Lee TL | 2005 | Cancer Res2005，65，16： | 1 |
| 16 | 2-Methoxyestradiol inhibits hypoxia-indueible factor 1α, tumor growth, and angiogenesis and augments paclitaxel efficary in head and neck squamous cell carcinoma显示文摘 | Ricker JL Chen Z Yang XP | 2004 | Clinical Cancer Res2004，10，24： | 1 |
| 17 | Inhibition of cyclooxygenase-2 improves cardiac function after myocardial infarction in the mouse显示文摘 | LaPointe MC Mendez M Leung A Tao Z Yang XP | 2004 | Am J Physiol Heart Circ Physiol2004，286，： | 1 |
| 18 | Diminished cardioproctective response to inhibition of angiotensin-converting enzyme and angiotensin Ⅱ type 1 receptor in 132 kinin receptor gene knockout mice显示文摘 | Yang XP Liu YH Dhaxmesh M | 2001 | Circ Res2001，88，： | 1 |
| 19 | Anticopper efficacy ofcaptopril and sodium dimercaptosulphonate in patients withilson's disease 显示文摘 | Wang XP Yang RM Ren MS | 2003 | Funct Neurol2003，18，3： | 1 |
| 20 | Phospholipid transfer protein is regulated by liver X receptors in vivo 显示文摘 | Cao G Beyer TP Yang XP | 2002 | J Biol Chem2002，277，39： | 1 |
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