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| 1 | Cloning and expression of MXR7 gene in human HCC tissue显示文摘AIM To clone and identify the whole cDNA ofMXR7 gene and to find out its expression inhuman HCC,and normal tissues.METHODS The DNA primers were designed andsynthesized according to the whole cDNAsequence of MXR? gene.The cDNA of humanHCC was taken as the template while the cDNA ofMXR7 gene was synthesized by polymerasechain reaction(PCR).Recombinant DNAconforming to reading frame was constructed byconnecting purified PCR product of the cDNA ofMXR? gene with expression vector pGEX-5X-1 offusion protein.The plasmid MXRT/pGEX-5X-1was identified by sequencing.Using 32p labeledMXR? cDNA as probe,MXR7 mRNA expressionwas detected by Northern blot analysis in 12different human normal tissues,7 preoperativelyuntreated non-liver tumor tissues,30preoperatively untreated HCC,theparacancerous liver tissues and 12 normal livertissues samples.RESULTS Restriction enzyme and sequenceanalysis confirmed that the insertion sequence invector pGEX-5X-1 was the same as the cDNAsequence of MXR7 gene.Northern blot analysisshowed no expression of MXR? mRNA in 12 kindsof normal human tissues including liver,7 tumortissues in other sites and 12 normal livertissues,the frequencies of MXR7 mRNA expression in HCC and paracancerous livertissues were 76.6% and 13.3%,respectively.The frequency of MXR7 mRNA expression in HCCwithout elevation of serum AFP and in HCC<5cm was 90%(9/10)and 83.3%(5/6),respectively.CONCLUSION MXR7 mRNA is highly expressedin human HCC,which is specific and occurs at anearly stage of HCC,suggesting MXR7 mRNA canbe a tumor biomarker for HCC.The detection ofMXR7 mRNA expression in the biopsied livertissue is helpful in discovering early subclinicalliver cancer in those with negative serum AFP. | Zhou XP Wang HY Yang GS Chen ZJ Li BA Wu MC | 2000 | World Journal of Gastroenterology2000，6，1： | 23 |
| 2 | miR-106b-25/miR-17-92 clusters: Polycistrons with oncogenic roles in hepatocellular carcinoma显示文摘MicroRNAs are small endogenously expressed RNA molecules which are involved in the process of silencing gene expression through translational regulation.The polycistronic miR-17-92 cluster is the first microRNA cluster shown to play a role in tumorigenesis.It has two other paralogs in the human genome,the miR-106b-25 cluster and the miR-106a-363 cluster.Collectively,the microRNAs encoded by these clusters can be further grouped based on the seed sequences into four families,namely the miR-17,the miR-92,the miR-18and the miR-19 families.Over-expression of the miR-106b-25 and miR-17-92 clusters has been reported not only during the development of cirrhosis but also subsequently during the development of hepatocellular carcinoma.Members of these clusters have also been shown to affect the replication of hepatitis B and hepatitis C viruses.Various targets of these microRNAs have been identified,and these targets are involved in tumor growth,cell survival and metastasis.In this review,we first describe the regulation of these clusters by c-Myc and E2F1,and how the members of these clusters inturn regulate E2F1 expression forming an auto-regulatory loop.In addition,the roles of the various members of the clusters in affecting relevant target gene expression in the pathogenesis of hepatocellular carcinoma will also be discussed. | Weiqi Tan Yang Li Seng-Gee Lim Theresa MC Tan | 2014 | World Journal of Gastroenterology2014，20，20： | 17 |
| 3 | Expression of perforin and granzyme B mRNA in judgement of immunosuppressive effect in rat liver transplantation显示文摘AIM To explore the expression of perform andgranzyme B genes mRNA to judge the effect ofimmunosuppression in acute rejection of livertransplantation.METHODS The expression of perform andgranzyme B genes mRNA was examined byreverse transcriptase-polymerase chain reaction(RT--PCR) in hamster to rat liver grafts under theimmunosuppression of cyclosporine or/andsplenectomy. Histological findings were studiedcomparatively.RESULTS Cyclosporine could obviouslydecrease the cellular infiltration, and completelyrepress the expression of mRNA for perform andgranzyme B, but could not change severehepatocyte necrosis and hemorrhage.SPlenectomy could significantly lightenhepatocyte necrosis, and completely eliminatehemorrhage, but not atfect the cellularinfiltration and the expression of perform andgranzyme B genes mRNA. Cyclosporine orsplenectomy alone could not prolong theSurvival time, however, their combination couldcompletely repress the rejection of liver grafts.The survival time of animals were significantlyprolonged (37.1 days ). The architecture ofhepatic lobules was preserved. There was slightcellular infiltration in the portal tracts and noexpression of perform and granzyme B genesmRNA could be seen in three weeks aftertransplantation.CONCLUSION Perform and granzyme B genesare valuable in judging the effect ofimmunosuppression in liver transplantation. | Zhang SG Wu MC Tan JW Chen H Yang JM Qian QJ | 1999 | World Journal of Gastroenterology1999，5，3： | 15 |
| 4 | Synthesis and Characterization of Mixed Al+AlN Nanoparticles显示文摘Mixed Al+AlN nanoparticles were synthesized by an active plasma-metal reaction method. Mean particle size of the mixed Al+AlN nanoparticles is about 30-50 nm. Defects were found in some AlN particles. Moreover, the AlN ratio and its particle size in mixed Al+AIN nanoparticles obtained in different atmospheres (N2+Ar) increase with increasing N2 particle pressure, and the specific sudece areas of the mixed Al+AlN nanoparticles increase with the AlN ratio. The surface degradation of the Al+AlN exposed to air is estimated by the infrared absorption | Sun, XC Yang, MC Ye, F Sun, XK Wei, WD Yu, YD Ping, DH | 1997 | Journal of Materials Science & Technology1997，13，4： | 2 |
| 5 | Biocompatibility and antibacterial activity of chitosan and collagen immobilized poly(3-hydroxybutyric acid-co-3- hydroxyvaleric acid) 显示文摘 | Hu SG Jou CH Yang MC | 2004 | Carthohydrate Polymer2004，58，2： | 1 |
| 6 | The roles of Muscarinic re- ceptor subtypes in modulation of nasal ciliary action 显示文摘 | Yang B Mc Caffrey T V | 1996 | Rhinology1996，34，3： | 1 |
| 7 | Protein adsorption,fibroblast activity and antibacterial properties of poly(3-hydroxybutyric acid-co-3-hydroxyvaleric acid) grafted with chitosan and chitooligosaccharide after immobilized with hyaluronic acid显示文摘 | Hu SG Jou CH Yang MC | 2003 | Biomaterials2003，24，16： | 1 |
| 8 | Curcumin induced apoptosis in immortalized NIH3T3 and malignant cancer cell lines 显示文摘 | Jiang MC Yang Yen HF Yen JJ | 1996 | Biochim Biophys Acta1996，26，1： | 1 |
| 9 | Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carci- noma patients following liver transplantation 显示文摘 | Yang Z Lai MC Xie HY | 2011 | Ann Surg Oneol2011，18，5： | 1 |
| 10 | The traditional Chinese herbal formula ASHMI inhibits allergic lung inflammationin antigen-sensitized and antigen-challenged aged mice显示文摘 | Busse P J Schofield B Birmingham N Yang N Wen MC Zhang T Srivastava K Li XM | | 0，，03： | 1 |
| 11 | Peptidergic modulation of mechanotrans- duction in rat arterial baroreceptors显示文摘 | Yang M Andresen MC | 1990 | Circ Res1990，66，： | 1 |
| 12 | Endothelial cells are damaged by au- tophagic induction before hepatocytes in Con A-induced acute hepatitis 显示文摘 | Yang MC Chang CP Lei HY | 2010 | Int Iramunol2010，22，8： | 1 |
| 13 | Measuring clinical attachmeng:reproducibility of relative measurements with an electronic probe显示文摘 | Clark WB Yang MC Magnusson I | 1992 | J Periodontol1992，63，10： | 1 |
| 14 | Long non - coding RNA MALAT -1overexpression predicts tumor recurrence of hepatocellular carcinomaafter liver transplantation 显示文摘 | Lai MC Yang Z Zhou L | 2012 | Med Oncol2012，29，3： | 1 |
| 15 | Myocardial infarct - sparing effect of adenosine A2A receptor activation is due to its action on CD4^+ T lymphocytes 显示文摘 | Yang Z Day YJ Toufektsian MC | 2006 | Circulation2006，114，19： | 1 |
| 16 | Can gene expression profiling predict survival for patients with squamous cell carcinoma of the lung显示文摘 | Sun Z Yang P Aubry MC | | 0，，01： | 1 |
| 17 | Ovarian sex cord-stromal tumors, pleuropulmonary blastoma and DICER1 mutations: a report from the International Pleuropulmonary Blastoma Registry 显示文摘 | Schultz KA Pacheco MC Yang J | 2011 | Gynecol Oncol2011，122，2： | 1 |
| 18 | Outcomes associated with delirium in older patients in surgical ICU显示文摘 | Balas MC Happ MB Yang W | 2009 | Chest2009，135，： | 1 |
| 19 | Effect of neonatal isolation on outcome following neonatal sei- zures in rats-the role of eorticosterone 显示文摘 | Lai MC Holmes GL Lee KH Yang SN Wang CA Wu CL | 2006 | Epilepsy Res2006，68，2： | 1 |
| 20 | Relationships between factor Ⅷ: Ag and factor Ⅷ in recombinant and plasma-derived factor Ⅷ concentrates 显示文摘 | Lin Y Yang X Chevrier MC | 2004 | Haemophilia2004，10，5： | 1 |
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