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| 1 | Clinical Features of Adult/Adolescent Atopic Dermatitis and Chinese Criteria for Atopic Dermatitis显示文摘 | Ping Liu Yan Zhao Zhang-Lei Mu Qian-Jin Lu Qian-Jin L U Li Zhang Xu Yao Min Zheng Yi-Wen Tang Xin-Xiano Lu Xiu-Juan xia You-Kun Lin Yu-Zhen Li Cai-Xia Tu Zhi-Rong Yao Jin-Hua Xu Wei Li Wei Lai Hui-Min Yang Hong-Fu Xie Xiu-Ping Han Zhi-Qiang Xie Xiang Nong Zai-Pei Guo Dan-Qi Deng Tong-Xin Shi Jian-Zhong Zhang | 2016 | Chinese Medical Journal2016，，7： | 64 |
| 2 | Transmission Dynamics of an Outbreak of the COVID-19 Delta Variant B.1.617.2——Guangdong Province,China,May-June 2021显示文摘On May 21,2021,a local case of coronavirus disease 2019(COVID-19)was confirmed in a 75-year-old woman(experienced onset of symptoms on May 18)in Liwan District,Guangzhou City,Guangdong Province,China.The number of infections has increased in the following 10 days and led to 5 generations of transmission. | Meng Zhang Jianpeng Xiao Aiping Deng Yingtao Zhang Yali Zhuang Ting Hu Jiansen Li Hongwei Tu Bosheng Li Yan Zhou Jun Yuan Lei Luo Zimian Liang Youzhi Huang Guoqiang Ye Mingwei Cai Gongli Li Bo Yang Bin Xu Ximing Huang Yazun Cui Dongsheng Ren Yanping Zhang Min Kang Yan Li | 2021 | China CDC weekly2021，3，27： | 51 |
| 3 | CD146 acts as a novel receptor for netrin-1 in promoting angiogenesis and vascular development显示文摘Angiogenesis,最新形成的血容器从先存在发芽的一个过程,为脊椎动物开发和成年动态平衡是重要的。以前的研究证明了 neuronal 指导分子 netrin-1 参予脉管的系统的 angiogenesis 和形态发生。Netrin-1 在 angiogenesis 展出双活动:支持或禁止 angiogenesis。netrin-1 的 anti-angiogenic 活动被 UNC5B 受体调停。然而, netrin-1 怎么支持 angiogenesis,仍然保持不清楚。这里,我们报导那 CD146,免疫球蛋白总科的 endothelial transmembrane 蛋白质,是为 netrin-1 的受体。Netrin-1 与高亲密关系绑在 CD146,导致 endothelial 房间激活并且以一种 CD146 依赖的方式的下游的发信号。在由特定的 anti-CD146 抗体的 netrin-CD146 相互作用的鼠科的内皮细胞层或混乱的 cd146 基因的有条件的大美人堵住或减少 netrin-1-induced angiogenesis。在 zebrafish 胚胎, downregulating 任何一个 netrin-1a 或 CD146 与惹人注目的类似导致脉管的缺点。而且,将宫外的脉管的发芽由 netrin-1 overexpression 导致了的 CD146 块击倒。一起,我们的数据揭开 CD146 在 angiogenesis 为 CD146 作为为 netrin-1 并且也的以前未知的受体揭示功能的 ligand,表明在脊椎动物开发期间在 angiogenesis 发信号的 netrin-CD146 的参与。 | Tao Tu Chunxia Zhang Huiwen Yan Yongting Luo Ruirui Kong Pushuai Wen Zhongde Ye Jianan Chen Jing Feng Feng Liu Jane Y Wu Xiyun Yan | 2015 | Cell Research2015，25，3： | 23 |
| 4 | Effects of dietary yeast β-glucan on nutrient digestibility and serum profiles in pre-ruminant Holstein calves显示文摘This study aimed to investigate the effects of dietary supplementation of yeast β-glucan on the nutrient digestibility and serum profiles in pre-ruminant Holstein calves. Forty-two neonatal Holstein calves((39.6±4.2) kg) were randomly allotted to six groups, and each was offered one of the following diets: a basal diet(control) or the basal diet supplemented with 25, 50, 75, 100 or 200 mg of yeast β-glucan kg–1 feed(dry matter basis). The basal diet consisted of a milk replacer and a starter feed. The trial lasted for 56 d. Two digestibility trials were conducted from d 14 to 20 and from d 42 to 48. Blood samples were collected on d 0, 14, 28 and 42 for serum profile analyses. On d 56, three calves from each group were slaughtered, and intestinal samples were collected to assess the villous height, crypt depth and mucosal thickness. Although feed intake was not affected by dietary treatment(P>0.05), the average daily gain(ADG) and gain-to-feed ratios were higher(P<0.05) for the calves fed 75 mg of yeast β-glucan kg–1 feed than those in the other groups. The supplementation of yeast β-glucan at 75 mg kg–1 feed increased the apparent digestibility of dry matter(DM), crude protein(CP), ether extract(EE), and phosphorus(P)(P<0.05) and the ratio of intestinal villous height to crypt depth(V/C)(P<0.05) when compared with the control group. No effects of yeast β-glucan on the serum concentrations of total protein(TP), albumin(ALB), serum urea nitrogen(SUN) and glucose(GLU) were observed(P>0.05). Compared with the control group, supplementation of yeast β-glucan decreased(P<0.05) the serum concentrations of triglycerides(TG) and total cholesterol(TC). The serum concentration of immunoglobulin G(Ig G) and immunoglobulin M(Ig M) increased quadratically(P<0.05), whereas the serum concentration of immunoglobulin A(Ig A) was unaffected by dietary treatments(P>0.05). The supplementation of yeast β-glucan stimulated the enzymatic activity of alkaline phosphatase(ALP)(P<0.05) compared with the control group. The lysozyme(LYZ) concentration increased quadratically(P<0.05) with increasing yeast β-glucan levels. The results suggested that dietary supplementation of yeast β-glucan at 75 mg kg–1 feed improved nutrient digestibility, enhanced immunity by increasing the immunoglobulin concentration and stimulating ALP, and exerted no adverse effects on metabolism in pre-ruminant calves. | MA Tao TU Yan ZHANG Nai-feng GUO Jiang-peng DENG Kai-dong ZHOU Yi YUN Qiang DIAO Qi-yu | 2015 | Journal of Integrative Agriculture2015，14，4： | 23 |
| 5 | Comparative Analysis on Chemical Components and Sensory Quality of Aging Flue-Cured Tobacco from Four Main Tobacco Areas of China显示文摘Complicated changes are happening during the aging process of flue-cured tobacco (FCT) and understanding of the changing components is of great significance in improving the quality,shortening aging time,and enhancing production and economic efficiency in tobacco industry.The aging samples of FCT from four main producing areas of China,i.e.,Yunnan,Sichuan,Hubei,and Dongbei,were used to study the changing rule of the components such as alkali,acid,and carbohydrate as well as the aroma and their relationship with sensory quality;and based on the correlation among the components and the sensory quality index,multivariable models were established to predict the aging quality.The results showed that the sensory quality of FCT increased steadily during the aging time,and at the same time,the acidic components (total organic acids,volatile acids) increased gradually,while the alkaline substances (nicotine,volatile alkali),carbohydrate (total sugars,reducing sugar) and pH values showed a downward trend.Correlation analysis found that the sensory quality and pH values were negatively correlated (P<0.05),while the sensory quality with total organic acids and aroma were positively correlated.The optimal model for predicting the quality of FCT was y=56.75-0.63x12+50.09x2-13.27x22,(y:sensory quality;x1:pH;x2:total organic acids).The average predicating error was 1.04% with maximum predicating error of 2.89% and the accuracy rate of above 97%. | SUN Jing-guo HE Jie-wang WU Feng-guang TU Shu-xing YAN Tie-jun SI Hui XIE Hao | 2011 | Agricultural Sciences in China2011，10，8： | 20 |
| 6 | Neuroprotection of Cyperus esculentus L. orientin against cerebral ischemia/reperfusion induced brain injury显示文摘Orientin is a flavonoid monomer.In recent years,its importance as a source of pharmacological active substance is growing rapidly due to its properties such as anti-myocardial ischemia,anti-apoptosis,anti-radiation,anti-tumor,and anti-aging.However,the neuroprotective effects of Orientin on stroke injury have not been comprehensively evaluated.The aim of the present study was thus to investigate the neuroprotective capacity and the potential mechanisms of Cyperus esculentus L.orientin(CLO)from Cyperus esculentus L.leaves against ischemia/reperfusion(I/R)injury using standard orientin as control.For in vitro studies,we treated HT22 cells with CoCl2 as an in vitro ischemic injury model.HT22 cells in the control group were treated with CoCl2.For in vivo studies,we used rat models of middle cerebral artery occlusion,and animals that received sham surgery were used as controls.We found that CLO protected CoCl2-induced HT22 cells against ischemia/reperfusion injury by lowering lipid peroxidation and reactive oxygen species formation as well as decreasing protein oxidation.However,CLO did not reduce the release of lactate dehydrogenase nor increase the activity of superoxide dismutase.Results showed that CLO could decrease neurological deficit score,attenuate brain water content,and reduce cerebral infarct volume,leading to neuroprotection during cerebral ischemia-reperfusion injury.Our studies indicate that CLO flavonoids can be taken as a natural antioxidant and bacteriostastic substance in food and pharmaceutical industry.The molecular mechanisms of CLO could be at least partially attributed to the antioxidant properties and subsequently inhibiting activation of casepase-3.All experimental procedures and protocols were approved on May 16,2016 by the Experimental Animal Ethics Committee of Xinjiang Medical University of China(approval No.IACUC20160516-57). | Si-Qun Jing Sai-Sai Wang Rui-Min Zhong Jun-Yan Zhang Jin-Zi Wu Yi-Xian Tu Yan Pu Liang-Jun Yan | 2020 | Neural Regeneration Research2020，15，3： | 16 |
| 7 | Environmental risks for application of iron and steel slags in soils in China: A review显示文摘Iron and steel slags are smelting wastes, mainly including blast furnace slag(BFS) and steel slag(SS) produced in the iron and steel industry. Utilization of iron and steel slags as resources for solving the problem of slag disposals has attracted much attention with increasing iron and steel smelting slags in China. Because the iron and steel slags contain calcium(Ca), magnesium(Mg), phosphorus(P), and silicon(Si), some have tried to use them as Si-and P-fertilizers, for producing Ca-Mg-P fertilizers, or as soil amendments in agriculture. However, in the iron metallurgical process, several pollutants in iron ores can inevitably transfer into iron and steel slags, resulting in the enrichment of pollutants both in BFS(mainly nickel(Ni), copper(Cu), mercury, zinc(Zn),cadmium(Cd), chromium(Cr), arsenic, lead, selenium, fluorine(F), and chlorine(Cl)) and in SS(mainly Ni, Cr, Cd, Zn, Cu, F, and Cl), in which some of pollutants(especially Cr, Ni, F, and Cl) exceed the limits of environmental quality standards for soils and groundwater. The elements of manganese, barium,and vanadium in iron and steel slags are higher than the background values of soil environment. In order to ensure soil health, food safety, and environmental quality, it is suggested that those industrial solid wastes, such as iron and steel slags, without any pretreatment for reducing harmful pollutants and with environmental safety risk, should not be allowed to use for soil remediation or conditioning directly in farmlands by solid waste disposal methods, to prevent pollutants from entering food chain and harming human health. | Xiaobin WANG Xiuying LI Xiang YAN Cheng TU Zhaoguo YU | 2021 | Pedosphere2021，31，1： | 14 |
| 8 | Effects of mulberry leaf flavonoid and resveratrol on methane emission and nutrient digestion in sheep显示文摘As a new type of methane control agent, natural plant extract has been widely studied in recent years,but in vivo studies are few. This study was to investigate the effects of the dietary supplementation of 2 different polyphenols on the methane(CH4) emission and digestion metabolism in sheep. Ten healthy crossbred sheep(Dorper(?)× small-tailed Han ♀; BW 60.0 ± 1.73 kg) were used in a change-over design.The sheep were fed the following 3 diets in the present study: the basal diet(CON) with no supplementation; the basal diet supplemented with 2 g mulberry leaf flavonoid(MLF) per day per sheep; the basal diet supplemented with 0.25 g resveratrol(RES) per day per sheep. Both MLF and RES reduced CH4 emission scaled to metabolic weight per kilogram of DMI and CO2 output scaled to metabolic weight, but the effect of RES was significant(P < 0.05). Both MLF and RES significantly improved apparent digestibility of DM, OM, NDF, ADF, and nitrogen, but the effect of RES was significant(P < 0.05). Both MLF and RES significantly improved ME(P < 0.05) and reduced energy losses in CH4 emission(P > 0.05). In conclusion, MLF and RES can improve the digestibility of nutrients, the utilization of nutrients and energy, and reduce CH4 emission, but they are not conducive to nitrogen retention. | Dandan Chen Xiaolin Chen Yan Tu Bo Wang Can Lou Tao Ma Qiyu Diao | 2015 | Animal Nutrition2015，，4： | 14 |
| 9 | Ionic liquid based dispersive liquid-liquid microextraction of aromatic amines in water samples显示文摘In this work,a new microextraction method termed ionic liquid based dispersive liquid-liquid microextraction(IL-DLLME) was demonstrated for the extraction of 2-methylaniline,4-chloroaniline,1-naphthylamine and 4-aminobiphenyl in aqueous matrices.After extraction the ionic liquid(IL)phase was injected directly into the high performance liquid chromatography(HPLC) system for determination.Some parameters that might affect the extraction efficiency were optimized.Under the optimum conditions,good linear relationship,sensitivity and reproducibility were obtained.The limits of detection(LOD,S/N=3)for the four analytes were in the range of 0.45-2.6μg L^(-1).The relative standard deviations(R.S.D.,n=6)were in the range of 6.2-9.8%. This method was applied for the analysis of the real water samples.The recoveries ranged from 93.4 to 106.4%.The main advantages of the method are high speed,high recovery,good repeatability and volatile organic solvent-free. | Yun Chang Fan Zheng Liang Hu Mei Lan Chen Chao Shen Tu Yan Zhu | 2008 | Chinese Chemical Letters2008，19，8： | 13 |
| 10 | CRISPR/Cas9-mediated PINK1 deletion leads to neurodegeneration in rhesus monkeys显示文摘Dear Editor,PINK1 mutations cause autosomal recessive and early-onset Parkinson’s disease(PD)with selective neurodegeneration.Unfortunately,current PINK1 knockout(KO)mouse1,2 and pig models3,4 are unable to recapitulate the selective and overt neurodegeneration seen in PD patients.Furthermore,endogenous Pink1 in the mouse brain is expressed at very low levels and can only be detected via immunoprecipitation,5 meaning that PINK1’s function in the mammalian brain needs to be assessed using larger animals that are closer to humans.We previously used CRISPR/Cas9 to target the monkey gene in one-cell stage embryos.6 Using the same approach,we designed two gRNAs to target exon 2(T1)and exon 4(T2),which encode a kinase domain in the PINK1 gene of rhesus monkeys(Fig.1a).CRISPR/Cas9 and PINK1 gRNAs were injected into one-cell stage rhesus monkey embryos.A T7E1 assay and sequencing of PCR products from the injected embryos showed high efficiency(61.5%)in targeting PINK1(Fig.1b and Supplementary information,Fig.S1).Transfer of 87 embryos to 28 surrogate rhesus monkeys resulted in 11 pregnancies(39.2%)(Fig.1b).Eleven fetuses developed to term and were born naturally.Of these live monkeys,eight carried PINK1 mutations(M),and three were wild type(WT).However,three mutant monkeys(M1,M3 and M4)were newborn triplets that struggled to survive and died 3-4 days after birth.One WT newborn monkey also died after a difficult labor.Another mutant monkey(M2)died 7 days after birth without noticeable warning signs or symptoms.The other three mutant monkeys(M6,M7 and M8)have lived for three years;M5,however,reduced its food intake and showed weakness at the age of 1.5 years,and died 30 days after anesthesia for MRI examination. | Weili Yang Yunbo Liu Zhuchi Tu Chong Xiao Sen Yan Xishan Ma Xiangyu Guo Xiusheng Chen Peng Yin Zhengyi Yang Su Yang Tianzi Jiang Shihua Li Chuan Qin Xiao-Jiang Li | 2019 | Cell Research2019，29，4： | 12 |
| 11 | Status of hyperhomocysteinemia in China: results from the China Stroke High-risk Population Screening Program, 2018显示文摘A nationwide survey was conducted from October 2018 to September 2019 to assess the prevalence of hyperhomocysteinemia(Hhcy)and its influencing factors in China.A standardized questionnaire was used to collect information.Hhcy was defined as the level of serum homocysteine(HCY)≥15.0µmol/L.The H-type hypertension(HHYP)was defined as hypertension with an elevated serum HCY(≥15.0µmol/L).Finally,110551 residents≥40 years of age from 31 provinces in the mainland of China were included.Overall,the median serum HCY level was 10.9µmol/L(interquartile range 7.9–15.1).A total of 28633 participants(25.9%)were defined as Hhcy.The Hhcy prevalence ranged from 7.9%in Shanghai to 56.8%in Tianjin.The data showed that serum HCY levels were associated with age,male gender,cigarette smoking,hypertension,diabetes,ethnicity,endurance in exercise(inverse),and fruit and vegetable intake(inverse).In addition,15486 participants were defined as HHYP,and the rate was 14.0%.HHYP was an independent predictor of stroke with an adjusted odds ratio of 1.752(95%CI 1.338–2.105).The geographical distribution pattern of the Hhcy epidemic reflects dynamic differences,and national strategies should be carried out to further improve the care of patients with Hhcy across China. | Wenjun Tu Feng Yan Baohua Chao Xunming Ji Longde Wang | 2021 | Frontiers of Medicine2021，15，6： | 12 |
| 12 | A Spatial Cluster Analysis of Heavy Rains in China显示文摘Clustered heavy rains (CHRs) defined using hierarchical cluster analysis based on daily observations of precipitation in China during 1960-2008 are investigated in this paper.The geographical pattern of CHRs in China shows three high-frequency centers-South China,the Yangtze River basin,and part of North China around the Bohai Sea.CHRs occur most frequently in South China with a mean annual frequency of 6.8 (a total of 334 times during 1960-2008).June has the highest monthly frequency (2.2 times/month with a total of 108 times during 1960-2008),partly in association with the Meiyu phenomenon in the Yangtze River basin.Within the past 50 years,the frequency of CHRs in China has increased significantly from 13.5 to 17.3 times per year,which is approximately 28%.In the 1990s,the frequency of CHRs often reached 19.1 times per year.The geographical extent of CHR has expanded slightly by 0.5 stations,and its average daily rainfall intensity has increased by 3.7 mm d 1.The contribution of CHRs to total rainfall amount and the frequency of daily precipitation have increased by 63.1% and 22.7%,respectively,partly due to a significant decrease in light rains.In drying regions of North and Northeast China,the amounts of minimal CHRs have had no significant trend in recent years,probably due to warming in these arid regions enhancing atmospheric convectivity at individual stations. | TU Kai YAN Zhong-Wei WANG Yi | 2011 | Atmospheric and Oceanic Science Letters2011，4，1： | 12 |
| 13 | Relations between sedimentary sequence and paleoclimatic changes during last 200 ka in the southern South China Sea显示文摘Core NS-93-5 was taken from the mild slope terrace of the southern South China Sea (SCS), which has preserved the steadiness depositional record of the normal marine environment since late Quaternary. Sedimentary sequence and oxygen isotopic stratigraphy of high resolution in the near 200 ka of the southern SCS has been established. By the comparative analysis with GISP2’s ice core, the depositional record of D/O’s events 1-21 and Heinrich’s events H1-H6 in the southern SCS that reflected the quick climate change in short time scale since the last interglacial stage is revealed, which indicates that in the last 200 ka in the southern SCS and the Arctic area there was tele-connection of paleoclimate and the unstability of the Western Pacific Warm Pool. This note shows that the sedimentary layers order of this sea area classified by color identification and by oxygen isotope stratum has the very good corresponding relation. The color feature of sediment changes along with the climate and the former | Muhong Chen Xia Tu Fan Zheng Wen Yan Xianzan Tang Jun Lu Baogui Wang Miao’an Lu | 2000 | Chinese Science Bulletin2000，45，14： | 12 |
| 14 | Associations of Gonadotropin-Releasing Hormone Receptor (GnRHR) and Neuropeptide Y(NPY) Genes’Polymorphisms with Egg-Laying Traits in Wenchang Chicken显示文摘Single nucleotide polymorphisms(SNP)of chicken gonadotropin-releasing hormone receptor(GnRHR)and neuropeptide Y(NPY)were selected to identify the genotypes of Wenchang(Chinese indigenous breed)chicken with restricton fragment length polymorphisms.The associations of the SNPs with the total egg production(NE),average days of continual laying(ADCL),and number of double-yolked eggs(DYE)traits were analyzed.The frequency of restriction enzyme A/a alleles in the population was for GnRHR 0.69(Bpu1102ⅠA)and 0.31(Bpu1102Ⅰa)and for NPY 0.46(Dra ⅠB)and 0.54(DraⅠb).Trait data from a total of 120 hens,which were purebredintroduced from Hainan Province,China from one generation were recorded.Two significant effects of genes'marker were found:for GnRHR and number of eggs (dominant;t=2.67,df=116)and NPY and number of eggs(additive;t=1.97,df=116).The current research supports the effects of GnRHR and NPY genes on egg-laying traits of chickens. | WU Xu LI Hui-fang YAN Mei-jiao TANG Qing-ping CHEN Kuan-wei WANG Jin-yu GAO Yu- shi TU Yun-jie YU Ya-bo ZHU Wen-qi | 2007 | Agricultural Sciences in China2007，6，4： | 11 |
| 15 | Current research progresses on calf rearing and nutrition in China显示文摘Calves are the reserve forces for dairy cattle. Scientific rearing strategy of calves is the basis of efficient cattle breeding. However, many problems exist in current rearing systems of calves and restrict the sustainable development of dairy cattle in China. The absence of basic research is the most highlighted problem among them. Recent researches on growth performance, nutrient digestibility, blood biochemical indices and rumen development in calves were summarized in this paper. Additionally, the optimal levels of energy and protein in milk replacer and starter diets for calves were indicated, and limiting amino acids for calves fed different diets were discussed. A variety of additives, such as acidifiers, probiotics and polysaccharides, are regarded as promising alternatives to antibiotics to reduce disease in calves. Dietary supplementations of these additives have positive effects on growth and health of calves. However, studies on the nutrition of vitamins and minerals in calves have been seldom done, and deserve our further researches. To sum up, the postnatal period is one of the most critical 'windows' for rumen manipulation and epigenetic regulation. Any changes from environments, especially early nutrition, may produce long-term effects on growth, health and milk yields in adult cattle. | DIAO Qi-yu ZhAng Rong Tu Yan | 2017 | Journal of Integrative Agriculture2017，16，12： | 10 |
| 16 | Effects of weaning age on growth, nutrient digestibility and metabolism,and serum parameters in Hu lambs显示文摘This study was conducted to investigate the effect of weaning age on growth performance, nutrient digestion and metabolism, and serological indicators, and to obtain an optimal weaning age in Hu lambs.Forty-eight newborn Hu lambs(birth weight, 2.53 ± 0.14 kg) were randomly divided into 4 groups. The lambs in control group(ER) suckled their dams. The lambs in other three experimental groups were weaned on milk replacer at 10.20, and 30 days of age(EW10, EW20, and EW30 groups),respectively. The results were as follows: 1) lambs in EW10 and EW30 groups had a lower(P < 0.05) ADG than those in ER group within 10 days post-weaning; the weaned lambs began to show a higher(P < 0.05) ADG than those in ER group after 20 days post-weaning. 2) EW10 and EW20 groups had a higher(P < 0.05) creep feed intake than EW30 and ER groups from 15 to 60 days of age. 3) The apparent digestibility of dry matter, organic matter, gross energy, nitrogen, ether extract and phosphorus, and the deposition of nitrogen and phosphorus did not differ(P > 0.05) among groups; however, the apparent digestibility and deposition of calcium in early weaned lambs were lower(P < 0.05) than those in ewe-reared lambs. 4)The albumin content in EW30 group was lower(P < 0.05) than that in ER group; the globulin content in EW30 group was higher(P < 0.05) than that in other groups; the content of serum insulin-like growth factor-1 in weaned lambs tended to increase compared with lambs in ER group. Finally, the growth rate of lambs decreased within 10 days post-weaning, but early weaning boosted creep feed intake, leading to better growth and health later in life. The Hu lambs can be weaned on milk replacer and creep feed at 10 days of age. | Jianmin Chai Qiyu Diao Haichao Wang Yan Tu Xiaojing Tao Naifeng Zhang | 2015 | Animal Nutrition2015，，4： | 10 |
| 17 | Effect of Different Protein Levels on Nutrient Digestion Metabolism and Serum Biochemical Parameters in Calves显示文摘当前的学习被执行了在生长,滋养的消化和吸收上检验不同饮食的蛋白质层次的效果,以及在小牛的生物化学的参数。九头健康新生的小牛被选择,随机划分了成 3 个组并且用不同蛋白质层次(18, 22,和 26%) 分别地喂了 3 牛奶 replacers。五 period-digestion-metabolism 试用被拿在之间 12 20, 22 30。32 40,在出生以后的 42 50,和 52 60 天。然而,所有 3 个组在 11 ~ 61 试验性的天期间显示出一条类似的生长曲线组是的 22% 粗蛋白(CP ) 的生长率 8.89% 高比 18% CP 的组织, 19.48% 高分别地比 26% CP 的组织。干燥材料(DM ) 的明显的消化的好坏与年龄逐渐地衰退了,而, N 的明显的消化的好坏。摘录醚(EE ) 稍微升起了。比作 22% CP 和 26% CP 组,显然用 18% CP 喂的小牛比 DM 有更低的消化的好坏。EE。并且氮(N) 。N 的平均明显的消化的好坏是 69.39, 75.36,和 74.55% 。分别地。钙( Ca )和磷(P)的明显的消化的好坏和保留在整个实验,而是在26% CP 组的 P 的平均明显的消化的好坏是稳定的仅仅是63.83%,它比18% CP 组(70.40%)和22% CP 组(69.73%)的显著地低。另外,全部的蛋白质(TP ) 的浆液集中,白朊(白长袍的) 。并且 22% CP 组的血球素(滴) 比在 18% CP 和 26% CP 组的那些高。在另一方面,在 18% CP 组的脲 N (甜面包) 内容比另外的二个组的显著地低。最高的葡萄糖(GLU ) 集中在 22% CP 组被发现(5.38 mmol L ? 1 ) ,在试用的结束。在牛奶 replacer 的蛋白质层次影响了营养和浆液的消化新陈代谢在不同生理的阶段的小牛的生物化学的参数。用 22% CP 牛奶 replacer 喂的小牛比另外的组有更好的生长表演。 | LI Hui DIAO Qi-yu ZHANG Nai-feng TU Yan WANG Ji-feng | 2008 | Agricultural Sciences in China2008，7，3： | 10 |
| 18 | Effects of Different Energy Levels on Nutrient Utilization and Serum Biochemical Parameters of Early-Weaned Calves显示文摘Current study was performed to investigate the effects of different energy levels on nutrient digestion metabolism and serum biochemical parameters of early-weaned calves. Twelve neonatal Holstein calves were selected and assigned randomly to receive 1 of 3 treatments that contained 18.51, 19.66 and 20.80 MJ kg-1 gross energy, respectively (n = 4 per treatment). Digestion and metabolism trials were carried out in 5 phases (12-20, 22-30, 32-40, 42-50, and 52-60 d of age). At the same time, blood metabolites were determined at 21, 31, 41, 51, and 61 d of age prior to morning feeding. The results indicated that either dry matter (DM), organic matter (OM) and gross energy (GE) apparent digestibility or N and P retention tended to increase as age advanced; the average value of DM and OM apparent digestibility and Ca retention in high-energy treatment was significantly lower than that of other groups (P < 0.05); there were no significant differences in the concentration of serum glucose, triglyceride and urea N among treatments, whereas a significant effect of age was found on the level of triglyceride (P < 0.05). To sum up, medium-energy milk replacer seems to be more beneficial for GE digestibility and N utilization, whereas low-energy milk replacer appears to be more rewarding for Ca and P utilization. | ZHANG Rong, DIAO Qi-yu, ZHANG Nai-feng, TU Yan and JIANG Cheng-gang Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China | 2010 | Agricultural Sciences in China2010，9，5： | 10 |
| 19 | Bioengineered miR-27b-3p and miR-328-3p modulate drug metabolism and disposition via the regulation of target ADME gene expression显示文摘Drug-metabolizing enzymes, transporters, and nuclear receptors are essential for the absorption, distribution, metabolism, and excretion(ADME) of drugs and xenobiotics. MicroRNAs participate in the regulation of ADME gene expression via imperfect complementary Watson–Crick base pairings with target transcripts. We have previously reported that Cytochrome P450 3A4(CYP3A4) and ATP-binding cassette sub-family G member 2(ABCG2) are regulated by miR-27b-3p and miR-328-3p,respectively. Here we employed our newly established RNA bioengineering technology to produce bioengineered RNA agents(BERA), namely BERA/miR-27b-3p and BERA/miR-328-3p, via fermentation. When introduced into human cells, BERA/miR-27b-3p and BERA/miR-328-3p were selectively processed to target miRNAs and thus knock down CYP3A4 and ABCG2 mRNA and their protein levels,respectively, as compared to cells treated with vehicle or control RNA. Consequently, BERA/miR-27b-3p led to a lower midazolam 10-hydroxylase activity, indicating the reduction of CYP3A4 activity. Likewise,BERA/miR-328-3p treatment elevated the intracellular accumulation of anticancer drug mitoxantrone, a classic substrate of ABCG2, hence sensitized the cells to chemotherapy. The results indicate that biologic miRNA agents made by RNA biotechnology may be applied to research on miRNA functions in the regulation of drug metabolism and disposition that could provide insights into the development of more effective therapies. | Xin Li Ye Tian Mei-Juan Tu Pui Yan Ho Neelu Batra Ai-Ming Yu | 2019 | Acta Pharmaceutica Sinica B2019，9，3： | 10 |
| 20 | Effect of supplementation of allicin on methanogenesis and ruminal microbial flora in Dorper crossbred ewes显示文摘Background: Garlic extracts have been reported to be effective in reducing methanogenesis. Related mechanisms are not well illustrated, however, and most studies have been conducted in vitro. This study investigates the effects of supplementary allicin(AL) in sheep diet on in vivo digestibility, rumen fermentation, and shifts of microbial flora.Methods: Two experiments were conducted using Dorper × thin-tailed Han crossbred ewes. In experiment 1,eighteen ewes(60.0 ± 1.73 kg BW) were randomly assigned for 29 days to either of two dietary treatments: a basal diet or the basal diet supplemented with 2.0 g AL/head?day to investigate supplementary AL on nutrient digestibility and methane emissions. In experiment 2, six ewes(65.2 ± 2.0 kg BW) with ruminal canulas were assigned to the same two dietary treatments as in experiment 1 for 42 days to investigate supplementary AL on ruminal fermentation and microbial flora. The methane emissions were determined using an open-circuit respirometry system and microbial assessment was done by q PCR of 16 S r RNA genes.Results: Supplementary AL increased the apparent digestibility of organic matter(P < 0.001), nitrogen(P = 0.006),neutral detergent fiber(P < 0.001), and acid detergent fiber(P = 0.002). Fecal nitrogen output was reduced(P = 0.001)but urinary nitrogen output was unaffected(P = 0.691), while nitrogen retention(P = 0.077) and nitrogen retention/nitrogen intake(P = 0.077) tended to increase. Supplementary AL decreased methane emissions scaled to metabolic bodyweight by 5.95 %(P = 0.007) and to digestible organic matter intake by 8.36 %(P = 0.009). Ruminal p H was unaffected(P = 0.601) while ammonia decreased(P = 0.024) and total volatile fatty acids increased(P = 0.024) in response to supplementary AL. Supplementary AL decreased the population of methanogens(P = 0.001) and tended to decrease that of protozoans(P = 0.097), but increased the populations of F. succinogenes(P < 0.001), R. flavefaciens(P = 0.001), and B. fibrisolvens(P = 0.001).Conclusions: Supplementation of AL at 2.0 g/head?day effectively enhanced OM, N, NDF, and ADF digestibility and reduced daily methane emissions(L/kg BW0.75) in ewes, probably by decreasing the population of ruminal protozoans and methanogens. | Tao Ma Dandan Chen Yan Tu Naifeng Zhang Bingwen Si Kaidong Deng Qiyu Diao | 2016 | Journal of Animal Science and Biotechnology2016，7，2： | 9 |
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