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| 1 | A deep learning-enabled portable imaging flow cytometer for cost-effective, highthroughput, and label-free analysis of natural water samples显示文摘We report a deep learning-enabled field-portable and cost-effective imaging flow cytometer that automatically captures phase-contrast color images of the contents of a continuously flowing water sample at a throughput of 100 mL/h.The device is based on partially coherent lens-free holographic microscopy and acquires the diffraction patterns of flowing micro-objects inside a microfluidic channel.These holographic diffraction patterns are reconstructed in real time using a deep learning-based phase-recovery and image-reconstruction method to produce a color image of each micro-object without the use of external labeling.Motion blur is eliminated by simultaneously illuminating the sample with red,green,and blue light-emitting diodes that are pulsed.Operated by a laptop computer,this portable device measures 15.5 cm×15 cm×12.5 cm,weighs 1 kg,and compared to standard imaging flow cytometers,it provides extreme reductions of cost,size and weight while also providing a high volumetric throughput over a large object size range.We demonstrated the capabilities of this device by measuring ocean samples at the Los Angeles coastline and obtaining images of its micro-and nanoplankton composition.Furthermore,we measured the concentration of a potentially toxic alga(Pseudo-nitzschia)in six public beaches in Los Angeles and achieved good agreement with measurements conducted by the California Department of Public Health.The cost-effectiveness,compactness,and simplicity of this computational platform might lead to the creation of a network of imaging flow cytometers for largescale and continuous monitoring of the ocean microbiome,including its plankton composition. | Zoltán Gӧrӧcs Miu Tamamitsu Vittorio Bianco Patrick Wolf Shounak Roy Koyoshi Shindo Kyrollos Yanny Yichen Wu Hatice Ceylan Koydemir Yair Rivenson Aydogan Ozcan | 2018 | Light(Science & Applications)2018，7，1： | 14 |
| 2 | One-passwavelet decompositions of data streams显示文摘 | Anna C G Yannis K Muthukrishnan S | 2003 | IEEE Transactions on Knowledge and Data Engineering2003，15，3： | 1 |
| 3 | Reduced synovial mem- brane macrophage numbers, ELAM-1 expression, and lining layer hyperplasia in psoriatic arthritis as compared with rheu- matoid arthritis显示文摘 | Veale D Yanni G Rogers S | 1993 | Arthritis Rheum1993，36，： | 1 |
| 4 | Generation of 60-GHz MB-OFDM Signal-Over-Fiber by Up-Conversion Using Cascaded External Modulators 显示文摘 | GIANG H N BEATRICE C YANNIS L G | 2009 | Journal of Lightwave Technology2009，27，912： | 1 |
| 5 | Increased numbers of CD5+ B cells and T cellreceptor gamma delta + T cells are associated with younger age of onset in rheumatoidarthritis显示文摘 | Hassan J Yanni G Hegarty V | 1996 | Clin Exp Immunol1996，103，： | 1 |
| 6 | Novel fiber - optic biosensor based on immobilized glutathione S - transferase and sol - gel entrapped bromcresol green for the determination of atrazine 显示文摘 | Vangelis G A Yannis D C | 2002 | Anal Chim Acta2002，460，： | 1 |
| 7 | Endogenous avidin-binding activity in epithelial cells of the ducts of human salivary glands显示文摘 | Cauli A Yanni G Panayi G S | 1994 | Clin Exp Rheumatol1994，12，： | 1 |
| 8 | Critical power two wheeler driving patterns at the emergence of an incident显示文摘 | Vlahogianni E 1 Yannis G Golias J C | 2013 | Accident Analysis and Prevention2013，58，： | 1 |
| 9 | Development of a diet-lifestyle quality index for young children and its relation to obesity:The preschoolers diet-iifestyle index显示文摘 | Yannis M Georgia K Evangelia G | 2010 | Public Health Nutrition2010，4，： | 1 |
| 10 | Production of an- giotensin converting enzyme by rheumatoid synovial mem- brane 显示文摘 | Veale D Yanni G Bresnihan B | 1992 | Ann Rheum Dis1992，51，47： | 1 |
| 11 | Studying participation networks in collaboration using mixed methods显示文摘 | Alejandra Martínez Yannis Dimitriadis Eduardo Gómez-Sánchez Bartolomé Rubia-Avi Iván Jorrín-Abellán Jose A. Marcos | 2006 | International Journal of Computer - Supported Collaborative Learning2006，，3： | 1 |
| 12 | Perception of Road Accident Causes 显示文摘 | Vanlaar W Yannis G | 2006 | Accident Analysis and Perception2006，38，1： | 1 |
| 13 | Glycogen synthase kinase - 313 inhibition induces nuclear factor - KB - mediated apoptosis in pediat- ric acute lymphocyte leukemia ceils显示文摘 | Yanni H Xiaoyan G Ruiyan L | 2010 | J Exp Clin Cancer Res2010，154，： | 1 |
| 14 | Novel liposome-based multicomponent systems for the protection of photolabile agents显示文摘 | Pramukh J Gregory G | 1995 | International Journal of Pharmaceutics1995，117，1： | 1 |
| 15 | Synovial tissue macrophages and joint erosions in rheumatoid artheitis显示文摘 | Yanni G Whelan A Ferghery C | 1994 | Ann Rheum Dis1994，53，1： | 1 |
| 16 | A criticalasvsevssment of pedestrian behaviour models 显示文摘 | Papadimitriou E Yannis G Golias J | 2009 | Transportation Research Part F2009，12，： | 1 |
| 17 | Modeling driver choices towards accident risk reduction显示文摘 | YANNIS G KANELLOPOULOU A AGGELOUSSI K | 2005 | Safety Science2005，43，3： | 1 |
| 18 | T2 lymphocyte immuophenotyping in polymyositis and dermatomyositis显示文摘 | Iannonef Cauli A Yanni G | 1996 | Br J Rheumatol1996，35，： | 1 |
| 19 | Bioactive microconstituents and antioxidant properties of wild edible mushrooms from the island of Lesvos, Greece 显示文摘 | KALOGEROPOULOS N YANNI A E KOUTROTSIOS G | 2013 | Food and chemical toxicology2013，55，： | 1 |
| 20 | Molecular interactions in crystallizing lysozyme solutions studied by photon correlation spectroscopy显示文摘 | Wolfram E Yannis G Wolfram S | 1994 | Journal of crystal growth1994，143，： | 1 |
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