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| 1 | Orchestration of energy metabolism and osteogenesis by Mg^(2+)facilitates low-dose BMP-2-driven regeneration显示文摘The clinical application of bone morphogenetic protein-2(BMP-2)is limited by several factors,including ineffectiveness at low doses and severe adverse effects at high doses.To address these efficacy and safety limitations,we explored whether orchestration of energy metabolism and osteogenesis by magnesium ion(Mg^(2+))could reduce the dose and thereby improve the safety of BMP-2.Our results demonstrated that rapid metabolic activation triggered by BMP-2 was indispensable for subsequent osteogenesis.Moreover,inadequate metabolic stimulation was shown to be responsible for the ineffectiveness of low-dose BMP-2.Next,we identified that Mg^(2+),as an'energy propellant',substantially increased cellular bioenergetic levels to support the osteogenesis via the Akt-glycolysis-Mrs2-mitochondrial axis,and consequently enhanced the osteoinductivity of BMP-2.Based on the mechanistic discovery,microgel composite hydrogels were fabricated as low-dose BMP-2/Mg^(2+)codelivery system through microfluidic and 3D printing technologies.An in vivo study further confirmed that rapid and robust bone regeneration was induced by the codelivery system.Collectively,these results suggest that this bioenergetic-driven,cost-effective,low-dose BMP-2-based strategy has substantial potential for bone repair. | Sihan Lin Shi Yin Junfeng Shi Guangzheng Yang Xutao Wen Wenjie Zhang Mingliang Zhou Xinquan Jiang | 2022 | Bioactive Materials2022，7，12： | 2 |
| 2 | Tunable Dynamic Black Phosphorus/Insulator/Si Heterojunction Direct-Current Generator Based on the Hot Electron Transport显示文摘Static heterojunction-based electronic devices have been widely applied because carrier dynamic processes between semiconductors can be designed through band gap engineering.Herein,we demonstrate a tunable direct-current generator based on the dynamic heterojunction,whose mechanism is based on breaking the symmetry of drift and diffusion currents and rebounding hot carrier transport in dynamic heterojunctions.Furthermore,the output voltage can be delicately adjusted and enhanced with the interface energy level engineering of inserting dielectric layers.Under the ultrahigh interface electric field,hot electrons will still transfer across the interface through the tunneling and hopping effect.In particular,the intrinsic anisotropy of black phosphorus arising from the lattice structure produces extraordinary electronic,transport,and mechanical properties exploited in our dynamic heterojunction generator.Herein,the voltage of 6.1 V,current density of 124.0 A/m^(2),power density of 201.0 W/m^(2),and energy-conversion efficiency of 31.4%have been achieved based on the dynamic black phosphorus/AlN/Si heterojunction,which can be used to directly and synchronously light up light-emitting diodes.This direct-current generator has the potential to convert ubiquitous mechanical energy into electric energy and is a promising candidate for novel portable and miniaturized power sources in the in situ energy acquisition field. | Yanghua Lu Sirui Feng Runjiang Shen Yujun Xu Zhenzhen Hao Yanfei Yan Haonan Zheng Xutao Yu Qiuyue Gao Panpan Zhang Shisheng Lin | 2019 | Research2019，，1： | 2 |
| 3 | Rabies Virus Pseudotyped with CVS-N2C Glycoprotein as a Powerful Tool for Retrograde Neuronal Network Tracing显示文摘Efficient viral vectors for mapping and manipulating long-projection neuronal circuits are crucial in structural and functional studies of the brain. The SAD strain rabies virus with the glycoprotein gene deleted pseudotyped with the N2 C glycoprotein(SAD-RV(DG)-N2 C(G)) shows strong neuro-tropism in cell culture, but its in vivo efficiency for retrograde gene transduction and neuro-tropism have not been systematically characterized.We compared these features in different mouse brain regions for SAD-RV-N2 C(G) and two other widely-used retrograde tracers, SAD-RV(DG)-B19(G) and r AAV2-retro. We found that SAD-RV(DG)-N2 C(G) enhanced the infection efficiency of long-projecting neurons by^10 times but with very similar neuro-tropism, compared with SAD-RV(DG)-B19(G). On the other hand, SAD-RV(DG)-N2 C(G) had an infection efficiency comparable with r AAV2-retro, but a more restricted diffusion range, and broader tropism to different types and regions of longprojecting neuronal populations. These results demonstrate that SAD-RV(DG)-N2 C(G) can serve as an effective retrograde vector for studying neuronal circuits. | Xutao Zhu Kunzhang Lin Qing Liu Xinpei Yue Huijie Mi Xiaoping Huang Xiaobin He Ruiqi Wu Danhao Zheng Dong Wei Liangliang Jia Weilin Wang Anne Manyande Jie Wang Zhijian Zhang Fuqiang Xu | 2020 | Neuroscience Bulletin2020，36，3： | 2 |
| 4 | Interfacial Built-In Electric Field-Driven Direct Current Generator Based on Dynamic Silicon Homojunction显示文摘Searching for light and miniaturized functional device structures for sustainable energy gathering from the environment is the focus of energy society with the development of the internet of things.The proposal of a dynamic heterojunction-based direct current generator builds up new platforms for developing in situ energy.However,the requirement of different semiconductors in dynamic heterojunction is too complex to wide applications,generating energy loss for crystal structure mismatch.Herein,dynamic homojunction generators are explored,with the same semiconductor and majority carrier type.Systematic experiments reveal that the majority of carrier directional separation originates from the breaking symmetry between carrier distribution,leading to the rebounding effect of carriers by the interfacial electric field.Strikingly,NN Si homojunction with different Fermi levels can also output the electricity with higher current density than PP/PN homojunction,attributing to higher carrier mobility.The current density is as high as 214.0 A/m^(2),and internal impedance is as low as 3.6 kΩ,matching well with the impedance of electron components.Furthermore,the N-i-N structure is explored,whose output voltage can be further improved to 1.3V in the case of the N-Si/Al2O3/N-Si structure,attributing to the enhanced interfacial barrier.This approach provides a simple and feasible way of converting low-frequency disordered mechanical motion into electricity. | Yanghua Lu Qiuyue Gao Xutao Yu Haonan Zheng Runjiang Shen Zhenzhen Hao Yanfei Yan Panpan Zhang Yu Wen Guiting Yang Shisheng Lin | 2020 | Research2020，，1： | 2 |
| 5 | Three-Dimensional Climatological Structures of the Arabian Sea Eddies and Eddy-Induced Flux显示文摘This study explores the spatial structure and transport characteristics of eddies in the Arabian Sea(AS)using Argo profiles and satellite measurements.The majority of eddies occur in the northern AS,especially along its northeastern boundary.In contrast,the western AS had a relatively higher eddy kinetic energy compared to the eastern part.Particularly,the strongest energetic eddies were present in the Somali Current system.The composite results revealed the evident thermohaline anomalies caused by cyclonic eddies(CEs)and anticyclonic eddies(AEs)in the upper 300m layers.The anomalous temperature structure within CEs and AEs showed a dominant dipole structure in the near-surface layer and a monopole structure below,with maximum temperature anomalies of approximately−0.8℃and+1.0℃located at depths of 100–150m,respectively.The composited salinity structures for CEs and AEs exhibited monopole vertical structures and sandwich-like patterns.For AEs,large positive salinity anomalies occurred at subsurface layers of 60–180 m with a peak value of about 0.07,and weak negative values were observed above 60m and below 180 m.A similar vertical structure but with an opposite sign operates for CEs.The composited CE and AE caused an equatorward salt flux with values of−8.1×10^(4)and−2.2×10^(4)kg s^(−1),respectively.CEs caused an equatorward heat flux of−7.7×10^(11)W,and AEs induced a poleward flux of 1.5×1011 W. | LIN Xinyu QIU Yun NI Xutao LIN Wenshu AUNG Cherry | 2023 | Journal of Ocean University of China2023，22，4： | 0 |
| 6 | Broadband Insulator-Based Dynamic Diode with Ultrafast Hot Carriers Process显示文摘The excitation,rebound,and transport process of hot carriers(HCs)inside dynamic diode(DD)based on insulators has been rarely explored due to the original stereotyped in which it was thought that the insulators are nonconductive.However,the carrier dynamics of DD is totally different from the static diode,which may bring a subverting insight of insulators. | Runjiang Shen Yanghua Lu Xutao Yu Qi Ge Huiming Zhong Shisheng Lin | 2023 | Research2023，，2： | 0 |
| 7 | Fibroblast growth factor 5 overexpression ameliorated lipopolysaccharide-induced apoptosis of hepatocytes through regulation of the phosphoinositide-3-kinase/protein kinase B pathway显示文摘Background:Sepsis is a systemic inflammatory syndrome induced by several infectious agents.Multiple organs are affected by sepsis,including the liver,which plays an important role in metabolism and immune homeostasis.Fibroblast growth factors(FGFs)participate in several biological processes,although the role of FGF5 in sepsis is unclear.Methods:In this study,lipopolysaccharide(LPS)was administrated to mice to establish a sepsis-induced liver injury.A similar in vitro study was conducted using L-02 hepatocytes.Western blot and immunohistochemistry staining were performed to evaluate the FGF5 expression level in liver tissues and cells.Inflammatory cell infiltrations,cleaved-caspase-3 expressions,reactive oxygen species and levels of inflammatory cytokines were detected by immunofluorescence,dihydroethidium staining,and reverse transcription quantitative polymerase chain reaction analysis,respectively.Flow cytometry was used to detect the apoptosis level of cells.In addition,ribonucleic acid(RNA)-sequencing was applied to explore the possible mechanism by which FGF5 exerted effects.Results:LPS administration caused FGF5 down-regulation in the mouse liver as well as in L-02 hepatocytes.Additionally,with FGF5 overexpression,liver injury and the level of hepatocyte apoptosis were ameliorated.Further,RNA sequencing performed in hepatocytes revealed the phosphoinositide-3-kinase/protein kinase B(PI3K/AKT)pathway as a possible pathway regulated by FGF5.This was supported using an inhibitor of the PI3K/AKT pathway,which abrogated the protective effect of FGF5 in LPSinduced hepatocyte injury.Conclusion:The anti-apoptotic effect of FGF5 on hepatocytes suffering from LPS has been demonstrated and was dependent on the activation of the PI3K/AKT signaling pathway. | Shengyu Cui Yuhua Li Xutao Zhang Bing Wu Ming Li Jixian Gao Lin Xu Hao Xia | 2022 | Chinese Medical Journal2022，135，23： | 0 |
| 8 | Polarized Water Driven Dynamic PN Junction-Based Direct-Current Generator显示文摘There is a rising prospective in harvesting energy from the environment,as in situ energy is required for the distributed sensors in the interconnected information society,among which the water flow energy is the most potential candidate as a clean and abundant mechanical source.However,for microscale and unordered movement of water,achieving a sustainable direct-current generating device with high output to drive the load element is still challenging,which requires for further exploration.Herein,we propose a dynamic PN water junction generator with moving water sandwiched between two semiconductors,which outputs a sustainable direct-current voltage of 0.3 V and a current of 0.64μA.The mechanism can be attributed to the dynamic polarization process of water as moving dielectric medium in the dynamic PN water junction,under the Fermi level difference of two semiconductors.We further demonstrate an encapsulated portable power-generating device with simple structure and continuous direct-current voltage output of 0.11 V,which exhibits its promising potential application in the field of wearable devices and the IoTs. | Yanghua Lu Yanfei Yan Xutao Yu Xu Zhou Sirui Feng Chi Xu Haonan Zheng Zunshan Yang Linjun Li Kaihui Liu Shisheng Lin | 2021 | Research2021，，1： | 0 |
| 9 | Altered gut microbiome in FUT2 loss-of-function mutants in support of personalized medicine for inflammatory bowel diseases显示文摘The FUT2 loss-of-function mutations are highly prevalent and are associated with inflammatory bowel disease(IBD).To investigate the impact of FUT2 loss-of-function mutation on the gut microbiota in patients with IBD,81 endoscopically confirmed IBD patients were genotyped and divided into 3 groups:homozygous for functional FUT2 genes(SeSe),with one copy of non-functional FUT2 gene(Sese),or homozygous for non-functional FUT2 genes(sese).Escherichia,which attaches to fucosylated glycoconjugates,was the only abundant genus exhibiting decreased abundance in sese patients.Compared with SeSe or Sese patients,sese patients exhibited higher abundance in CD8+inducing Alistipe and Phascolarctobacterium and Th17 inducing Erysipelotrichaceae UCG-003.Counter-intuitively,butyrate-producing bacteria were more abundant in sese patients.Consistently,metabolomics analysis found higher levels of butyrate in sese patients.Our data support the hypothesis that FUT2 loss-of-function mutation participates in the IBD pathogenesis by decreasing binding sites for adherent bacteria and thus altering the gut microbiota.Decreased abundances of adherent bacteria may allow the overgrowth of bacteria that induce inflammatory T cells,leading to intestinal inflammation.As FUT2 loss-of-function mutations are highly prevalent,the identification of T cell inducing bacteria in sese patients could be valuable for the development of personalized microbial intervention for IBD. | Sijing Cheng Jun Hu Xianrui Wu Ji-An Pan Na Jiao Yichen Li Yibo Huang Xutao Lin Yifeng Zou Yuan Chen Lixin Zhu Min Zhi Ping Lan | 2021 | Journal of Genetics and Genomics2021，48，9： | 0 |
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