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| 1 | Brain cell apoptosis and enhancement of nervous excitability in pregnant rats with high plasma levels of homocysteine显示文摘Hyperhomocysteinemia is an important risk factor for preeclampsia-eclampsia. This study established a pregnant rat model of hyperhomocysteinemia, in which blood plasma homocysteine concentrations were twice or three times greater than that of normal pregnant rats. TUNEL revealed an increase in the number of apoptotic cells in the frontal cortex of pregnant rats with hyperhomocysteinemia. In addition,immunohistochemical staining detected activated nuclear factor-κB-positve cells in the frontal cortex.Reverse transcription-PCR detected that mRNA expression of the anti-apoptotic gene bcl-2 diminished in the frontal cortex. In situ hybridization and western blotting revealed that N-methyl-D-aspartate receptor 1 mRNA and protein expression was upregulated in the frontal cortex and hippocampus. These results indicate that hyperhomocysteinemia can induce brain cell apoptosis,increase nerve excitability, and promote the occurrence of preeclampsia in pregnant rats. | Jun Wang Jing Ge Liu Yang Haiyan Zhang Xuli Li Dan Xue | 2012 | Neural Regeneration Research2012，7，28： | 2 |
| 2 | Combustion synthesis and photoluminescentce of SrAl2O4:Eu, Dy phosphor nanopaticles 显示文摘 | Tianyou Peng Huanping Yang Xuli Pu | 2004 | Materials Letter2004，58，2： | 1 |
| 3 | Abnormal expression of VEGF and its gene transcription status as diagnostic indicators in patients with non-small cell lung cancer显示文摘Objective Angiogenesis is known to be essential for the survival,growth,invasion,and metastasis of lung cancer cells. Vascular endothelial growth factor(VEGF) is an important factor regulating angiogenesis of non-small cell lung cancer(NSCLC); however,its pathologic features and significance are unclear. In this study,the tissue VEGF expression levels and its gene transcriptional status,as well as circulating VEGF levels,were investigated in patients with lung disease. Methods VEGF protein and m RNA expression levels in 38 lung tissue samples were analyzed by immunohistochemistry and reverse transcription-polymerase chain reaction(RT-PCR),respectively. Circulating VEGF levels were detected quantitatively by an enzyme linked immuno-sorbent assay. Results The level of VEGF expression was significantly higher in lung cancer tissue than in the corresponding paracancerous or non-cancerous tissues. The average level of VEGF-positive staining was 76% in tissue samples from NSCLC patients; the levels were 89% in tissue samples from stage III patients and 92% in stage IV patients. High VEGF expression was also evident in cases with lymph node metastasis(84%),distant metastasis(90%),and lower differentiation degree(89%). VEGF m RNA in cancerous tissues was represented predominantly by the VEGF121 and VEGF165 isoforms. Circulating VEGF levels were significantly higher in NSCLC patients [(840 ± 324) pg/m L] than in patients with benign lung diseases [(308 ± 96) pg/m L] or in healthy individuals serving as controls [(252 ± 108) pg/m L]. Conclusion The over-expression of lung VEGF and its gene transcription status should be useful molecular indicators for NSCLC diagnosis. | Yun Shi Yang Shi Xuli Yang Jianrong Chen Qi Qian Dengfu Yao Guangzhou Wu | 2015 | Oncology and Translational Medicine2015，1，5： | 1 |
| 4 | 显示文摘 | Peng Tianyou Yang Huanping Pu Xuli | 2004 | Materials Letters2004，58，： | 1 |
| 5 | 显示文摘 | Peng Tianyou Yang Huanping Pu Xuli | 2004 | Materials Letters2004，58，： | 1 |
| 6 | Combustion synthesis and photoluminescence of SrA1204 : Eu, Dy phos- phor nanoparticles显示文摘 | Peng Tianyou Yang Huanping Pu Xuli | 2004 | Mater Lett2004，58，34： | 1 |
| 7 | Novel Wearable Energy Devices Based on Aligned Carbon Nanotube Fiber Textiles显示文摘 | Shaowu Pan Huijuan Lin Jue Deng Peining Chen Xuli Chen Zhibin Yang Huisheng Peng | 2015 | Adv. Energy Mater2015，，： | 1 |
| 8 | 显示文摘 | Peng Tianyou Yang Huanping Pu Xuli | 2004 | Materials Letter2004，58，2： | 1 |
| 9 | Combustion synthesis and photoluminescentce of SrAl2O4:Eu,Dy phosphor nanopaticles显示文摘 | Tianyou Peng Huanping Yang Xuli Pu | 2004 | Materials Letter2004，58，2： | 1 |
| 10 | Clinicopathological features of hypoxia-inducible factor-1α and vascular endothelial growth factor expression in patients with lung cancer显示文摘Objective The aim of the study was to investigate the clinicopathological characteristics of hypoxiainducible factor-1α(HIF-1α) and vascular endothelial growth factor(VEGF) expression in patients with lung cancer.Methods Cancerous and noncancerous tissues were collected post-operation from 115 patients with lung cancers by the self-control method. Total RNA was extracted from the lung tissues. The status of tissue HIF-1α expression and intercellular distribution was observed by immunochemistry using a tissue microarray. The expression levels of circulating HIF-1α and VEGF were detected by enzyme-linked immunosorbent assay(ELISA).Results The expression of serum HIF-1α [(138.3 ± 28.8) μg/L] in the group of patients with lung cancer was significantly higher(P < 0.01) than that in the group of patients with pneumonia [(58.8 ± 14.5) μg/L] and the control group of patients ((24.1 ± 3.3) μg/L)There was a strong positive correlation of serum HIF-1α levels(r = 0.937, P < 0.01) with serum VEGF levels. The specific concentration of total RNA [(1.52 ± 1.14) μg/mg wet lung tissues] in the cancerous tissues was significantly higher(t = 8.494, P < 0.001) than that in the noncancerous tissues ((0.58 ± 0.33) μg/mg)The clinicopathological features of HIF-1α expression in lung cancer tissues revealed a significant relationship between positive HIF-1α expression and patient sex(χ~2 = 4.494, P = 0.034), tumor size(χ~2 = 4.679, P = 0.031), differentiation degree(χ~2= 8.846, P = 0.012), and presence of lymphatic node metastasis(χ~2= 6.604, P = 0.037).Conclusion Abnormal HIF-1α expression in lung cancer is closely related with nucleic acid metabolism and angiogenesis, and it may be helpful in the diagnosis and identification of lung cancer. | Xuli Yang Li Wang Wenli Sai Yin Cai Juanjuan Gu Xin Chen Dengfu Yao | 2016 | Oncology and Translational Medicine2016，2，6： | 1 |
| 11 | Combustion synthesis and photoluminescenee of SrAl2O4:Eu, Dy phosphor nanoparticles显示文摘 | Peng Tianyou Yang Huanping Pu Xuli | 2004 | Materials Letters2004，58，： | 1 |
| 12 | Combustion synthesis and photoluminescentce of SrAl2O4:Eu, Dy phosphor nanopaticles 显示文摘 | PENG Tianyou YANG Huanping PU Xuli | 2004 | Mater Lett2004，58，35： | 1 |
| 13 | Soil C, N and P stocks and stoichiometry under different vegetation on the surface of the Leshan Giant Buddha显示文摘The accumulation of soil organic matter and nutrients is an important pathway in effectivelyunderstanding the mechanisms of plant settlement and rock weathering, while the characteristics ofsoil organic carbon (C), nitrogen (N) and phosphorus (P) under different vegetation remain unclear.In this study, the stocks and stoichiometry of soil organic C, N and P were determined in differentpositions and types of vegetation on the surface of the Leshan Giant Buddha. We found that the totalstocks of soil organic C, N and P were 1689.77, 134.6 and 29.48 kg, respectively, for the Buddha.The stocks of soil organic C, N and P under vascular plants were higher than those under othervegetation, with highest values observed under herb. Higher stocks per unit area (m2) of soil organicC, N and P were found on the left and right arms, shoulders, and two platforms. These results providea full primary picture in understanding soil organic C, N and P accumulation and distribution on thesurface of the Buddha, which could supply the fundamental data on weathering management of theBuddha and other similar open-air stone carvings. | Fujia Wu Wanqin Yang Bo Sun Tianyu Yang Xuli Chen Zhenfeng Xu Huixing Song | 2022 | Soil Ecology Letters2022，4，1： | 1 |
| 14 | Combustion synthesis and photoluminescence of SrAl2O4:Eu,Dy phosphor nanoparticles显示文摘 | Yang Huanping Pu Xuli | 2004 | Materials Letters2004，58，34： | 1 |
| 15 | Application of ionic liquids in CO_(2)capture and electrochemical reduction:A review显示文摘As a new type of green solvent with non-volatility,high thermal stability,high conductivity and various adjustable properties,ionic liquid(IL)has been widely used in the capture and electrochemical reduction of carbon dioxide(CO_(2)).To date,many studies have been made to investigate CO_(2)capture by using different types of ILs and CO_(2)electrochemical reduction(CO_(2)ER)with ILs as either electrolyte or other catalytic active components.Some structure-activity relationships between the structure and adsorption or catalytic properties of ILs have been found.Herein,the absorption performances and mechanisms of conventional ILs,amino-functionalized ILs,non-amino functionalized ILs and supported ILs for CO_(2)capture,as well as the performances and action mechanisms of ILs as the electrolyte,electrolyte additive,and/or electrode modifier in the process of CO_(2)ER are summarized.Many researches indicate that the unique interaction between the anion or cation of IL and CO_(2)has a significant contribution to promote the absorption and conversion of CO_(2).However,the ILs used for CO_(2)capture and electrochemical reduction should be further explored.Especially,a more in-depth investigation of the adsorption and catalytic mechanisms with the help of quantum chemical calculation,molecular simulation,and in situ characterization techniques is necessary.It is expected to design and develop more efficient ILs used for CO_(2)capture and conversion on a large scale. | Xiaowei An Peifen Wang Xuli Ma Xiao Du Xiaogang Hao Ziyuan Yang Guoqing Guan | 2023 | Carbon Resources Conversion2023，6，2： | 0 |
| 16 | Differential bone metabolism and protein expression in mice fed a high-fat diet versus Daurian ground squirrels following natural pre-hibernation fattening显示文摘This study compared the effects on bone metabolism and morphology of pathological obesity induced by excessive fat intake in a non-hibernator(mice)versus healthy obesity due to pre-hibernation fattening in a hibernator(ground squirrels).Kunming mice were fed a high-fat diet to provide a model of pathological obesity(OB group).Daurian ground squirrels fattened naturally in their pre-hibernation season(PRE group)were used as a healthy obesity model.Micro-computed tomography(micro-CT)and three-point bending tests were used to determine the microstructure and mechanical properties of bone.Western blots were used to analyze protein expression levels related to bone metabolism(Runt-related transcription factor 2(RunX2),osteocalcin(OCN),alkaline phosphatase(ALP),osteoprotegerin(OPG),receptor activator of nuclear factor-κB ligand(RANKL),cathepsin K,matrix metallopeptidase 9(MMP9),patched protein homolog 1(Ptch1),phosphorylatedβ-catenin(P-β-catenin),and glycogen synthase kinase-3β(GSK-3β)).Compared with controls,there was no obvious bone loss in the OB mice,and the stiffness of the femur was increased significantly.Compared with summer active squirrels,bone formation was enhanced but the mechanical properties did not change in the PRE group squirrels.In OB mice,western blots showed significantly increased expression levels of all proteins except RunX2,OPG,and Ptch1.PRE ground squirrels showed significantly increased expression of most proteins except OCN and Ptch1,which decreased significantly,and P-β-catenin and OPG,which did not change.In conclusion,for non-hibernating mice,moderate obesity had a certain protective effect on bones,demonstrating two-way regulation,increasing both bone loss and bone formation.For pre-hibernating ground squirrels,the healthy obesity acquired before hibernation had a positive effect on the microstructure of bones,and also enhanced the expression levels of proteins related to bone formation,bone resorption,and Wnt signaling. | Xuli GAO Shenyang SHEN Qiaohua NIU Weilan MIAO Yuting HAN Ziwei HAO Ning AN Yingyu YANG Yu ZHANG Han ZHANG Kenneth BSTOREY Hui CHANG | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，12： | 0 |
| 17 | Identification of potential anti-pneumonia pharmacological components of Glycyrrhizae Radix et Rhizoma after the treatment with Gan An He Ji oral liquid显示文摘Glycyrrhizae Radix et Rhizoma,a traditional Chinese medicine also known as Gan Cao(GC),is frequently included in clinical prescriptions for the treatment of pneumonia.However,the pharmacological components of GC for pneumonia treatment are rarely explored.Gan An He Ji oral liquid(GAHJ)has a simple composition and contains GC liquid extracts and paregoric,and has been used clinically for many years.Therefore,GAHJ was selected as a compound preparation for the study of GC in the treatment of pneumonia.We conducted an in vivo study of patients with pneumonia undergoing GAHJ treatments for three days.Using the intelligent mass spectrometry data-processing technologies to analyze the metabolism of GC in vivo,we obtained 168 related components of GC in humans,consisting of 24 prototype components and 144 metabolites,with 135 compounds screened in plasma and 82 in urine.After analysis of the metabolic transformation relationship and relative exposure,six components(liquiritin,liquiritigenin,glycyrrhizin,glycyrrhetinic acid,daidzin,and formononetin)were selected as potential effective components.The experimental results based on two animal pneumonia models and the inflammatory cell model showed that the mixture of these six components was effective in the treatment of pneumonia and lung injury and could effectively downregulate the level of inducible nitric oxide synthase(iNOS).Interestingly,glycyrrhetinic acid exhibited the strongest inhibition on iNOS and the highest exposure in vivo.The following molecular dynamic simulations indicated a strong bond between glycyrrhetinic acid and iNOS.Thus,the current study provides a pharmaceutical basis for GC and reveals the possible corresponding mechanisms in pneumonia treatment. | Xiaojuan Jiang Yihua Lin Yunlong Wu Caixia Yuan Xuli Lang Jiayun Chen Chunyan Zhu Xinyi Yang Yu Huang Hao Wang Caisheng Wu | 2022 | Journal of Pharmaceutical Analysis2022，12，6： | 0 |
| 18 | Programmable dual responsive system reconstructing nerve interaction with small-diameter tissue-engineered vascular grafts and inhibiting intimal hyperplasia in diabetes显示文摘Small-diameter tissue-engineered vascular grafts(sdTEVGs)with hyperglycemia resistance have not been constructed.The intimal hyperplasia caused by hyperglycemia remains problem to hinder the patency of sdTEVGs.Here,inspired by bionic regulation of nerve on vascular,we found the released neural exosomes could inhibit the abnormal phenotype transformation of vascular smooth muscle cells(VSMCs).The transformation was a prime culprit causing the intimal hyperplasia of sdTEVGs.To address this concern,sdTEVGs were modified with an on-demand programmable dual-responsive system of ultrathin hydrogels.An external primary Reactive Oxygen Species(ROS)-responsive Netrin-1 system was initially triggered by local inflammation to induce nerve remolding of the sdTEVGs overcoming the difficulty of nerve regeneration under hyperglycemia.Then,the internal secondary ATP-responsive DENND1A(guanine nucleotide exchange factor)system was turned on by the neurotransmitter ATP from the immigrated nerve fibers to stimulate effective release of neural exosomes.The results showed nerve fibers grow into the sdTEVGs in diabetic rats 30 days after transplantation.At day 90,the abnormal VSMCs phenotype was not detected in the sdTEVGs,which maintained long-time patency without intima hyperplasia.Our study provides new insights to construct vascular grafts resisting hyperglycemia damage. | Yanzhao Li Yeqin Wang Fangchao Xue Xuli Feng Zhaojing Ba Junjie Chen Zhenhua Zhou Yanhong Wang Ge Guan Guanyuan Yang Ziwei Xi Hao Tian Yong Liu Ju Tan Gang Li Xiewan Chen Mingcan Yang Wen Chen Chuhong Zhu Wen Zeng | 2022 | Bioactive Materials2022，7，1： | 0 |
| 19 | Analysis of Biological Activity and Immunogenicity of Extracellular Products from Streptococcus iniae显示文摘[Objectives] This study aimed to analyze the biological activity and immunogenicity of extracellular products from Streptococcus iniae. [Methods] S.iniae was incubated with brain heart infusion agar medium( BHIA + 4% calf serum) for 60 h. The bacterial liquid was rinsed with PBS,centrifuged,and filtered through microporous filtering film to collect extracellular products( ECPs). [Results]Extracellular proteinase( ECPase) of S. iniae exhibited amylase,protease,lecitinase,gelatinase,lipase activities and hemolytic activity but had no urease activity. EDTA,DTT and PMSF could reduce ECPase activity to 72. 4%,77. 6%and 72. 4%,respectively. Cu^(2+),Ca^(2+),K+and Mg^(2+)exhibited an inhibitory effect on ECPase activity,whereas Fe^(3+),Co^(2+)and Mn^(2+)could activate ECPase activity. ECPs had good heat stability and exhibited relatively high activities under alkaline conditions. The optimal temperature for ECPs was 55 ℃. Two-dimensional electrophoresis was performed to analyze the main protein of ECPs. The results indicated that there are 12 main bands of ECPs,and the molecular weights mainly ranged between 28-68 kD a. About 120 protein spots were detected,and the molecular weights mainly ranged between 26-95 kD a. The mouse anti-S. iniae was used for Western-blot analysis of ECPs,and the results showed that there were four proteins,with molecular weights of 26,37,95,and 97 kD a,respectively. The pathogenicity assay indicated that ECPs of S. iniae were highly pathogenic to tilapia. The mortality rate of tilapia was enhanced as the concentration of ECPs increased. [Conclusions] This study provided a certain theoretical basis for revealing the pathogenic mechanism of Streptococcus iniae. | Danling GUO Shihui ZHOU Siyao ZHENG Dawei SONG Xuli ZHANG Huanying PANG Peiwen WU Jichang JIAN Yang HUANG | 2018 | Agricultural Biotechnology2018，7，4： | 0 |
| 20 | Effect of lipid peroxidation on the allergenicity and functional properties of soybeanβ-conglycinin(7S)and glycinin(11S)显示文摘This study aimed to analyze the effect of lipid peroxidation on the allergenicity and functional properties of soybeanβ-conglycinin(7 S)and glycinin(11 S).Oxidation complexes were determined using the lipid peroxidation method.Functional properties were analyzed based on emulsifying and foaming properties.The potential allergenicity was evaluated by in vitro and in vivo methods.The results found that oxidation altered structures of the proteins and resulted in the formation of cross-linked protein polymers.The emulsion and foaming properties of the proteins were improved after oxidation.The IgE-binding capacity of 7 S and11 S reduced after oxidation.KU812 cell assays showed that both histamine and IL-4 release decreased after oxidation treatment.A mouse model showed that oxidation reduced the IgE,IgG,and IgG1 levels,as well as reduced histamine and mMCP-1 release in serum,which might suppress the allergic reaction.In conclusion,the lipid peroxidation treatment likely causes changes to the functional properties of soybean,decreasing the potential allergenicity of 7 S and 11 S. | Liying Ye Liangtao Lv Xiao Lin Kan He Xiaoquan Yang Zhili Wan Lizhong Liu Haiqian Wu Shaojun Xing Xuli Wu | 2023 | Food Science and Human Wellness2023，12，3： | 0 |
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