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| 1 | Effects of dietary Clostridium butyricum supplementation on growth performance,intestinal development,and immune response of weaned piglets challenged with lipopolysaccharide显示文摘Background: Weanling pigs, with immature immune system and physiological function, usually experience postweaning diarrhea. This study determined the effects of dietary Clostridium butyricum supplementation on growth performance, diarrhea, and immunity of weaned pigs challenged with lipopolysaccharide(LPS).Methods: In Experiment(Exp.) 1,144 weaned piglets were weaned at 21 d and randomly assigned to six groups,with six replicates per group and four pigs per replicate, receiving a control diet(CON) or diet supplemented with antibiotics(AB) or C. butyricum(CB)(0.1%, 0.2%, 0.4%, or 0.8%), respectively. All diets in Exp. 1 were a highly digestible basal diet, with 3,000 mg/kg zinc oxide supplied in the first 2 wk only. In Exp. 2, 180 piglets were weaned at 21 d and randomly assigned to five groups, with six replicates per group and six pigs per replicate, receiving CON, AB, or CB(0.2%, 0.4%, or 0.6%) diets. The digestibility of diets was lower than those in Exp. 1, and did not include zinc oxide. At 36 d of Exp. 2, 12 piglets were selected from each of the CON and 0.4% CB groups, six piglets were intraperitoneally injected with LPS(50 μg/kg body weight) and the other six piglets with normal saline;animals were killed at 4 h after injection to collect blood, intestine, and digesta samples for biochemical analysis.Results: In Exp. 1, CB and AB diets had no effect on growth performance of piglets. In Exp. 2, 0.4% CB decreased feed-gain ratio(P < 0.1), diarrhea score(P < 0.05), and increased duodenal, jejunal, and ileal villus height and jejunal villus height/crypt depth(P < 0.05). The 0.4% CB decreased the plasma tumor necrosis factor(TNF) α(P < 0.05) but increased ileal mucosa IL-10 and TLR2 mRNA expression(P < 0.05). Furthermore, 0.4% CB altered the microbial profile, with Bacillus and Ruminococcaceae UGG-003 at genus level and Lactobacillus casei and Parasutterella secunda at species level were higher than CON in colonic content(P < 0.05).Conclusions: Dietary C. butyricum supplementation had positive effects on growth of weaned piglets with less digestible diets. There was a tendency to reduce the feed-gain ratio, which could reduce feed costs in pig production. Moreover, C. butyricum decreased post-weaning diarrhea by improving the intestinal morphology,intestinal microflora profile, and immune function. | Ling Chen Shuang Li Jie Zheng Wentao Li Xuemei Jiang Xilun Zhao Jian Li Lianqiang Che Yan Lin Shengyu Xu Bin Feng Zhengfeng Fang De Wu | 2018 | Journal of Animal Science and Biotechnology2018，9，4： | 26 |
| 2 | Molecular mechanism of SCARB2-mediatec attachment and uncoating of EV71显示文摘 | Minghao Dang Xiangxi Wang Quan Wang Yaxin Wang Jianping Lin Yuna Sun Xuemei Li Liguo Zhang Zhiyong Lou Junzhi Wang Zihe Rao | 2014 | Protein & Cell2014，5，9： | 13 |
| 3 | Effects of dietary supplementation with essential oils and protease on growth performance,antioxidation,inflammation and intestinal function of weaned pigs显示文摘This experiment evaluated the impacts of essential oils(EO)and protease(PRO),independently or in combination,on growth performance,antioxidation,inflammation and intestinal function of weaned pigs.One hundred and sixty weaned pigs(21 d of age,BW of 6.74±0.20 kg)were randomly divided into 4 treatments with 8 replicate pens of 5 pigs per pen.Dietary treatments included the following:1)control diet(CON),2)CON with 300 mg/kg essential oils(EO),3)CON with 500 mg/kg protease(PRO),4)CON with 300 mg/kg essential oil and 500 mg/kg protease(EO + PRO).On d 8,one pig from each penwas selected for sampling.The remaining pigs were fed for an additional week and growth performance was monitored during this period.Dietary treatments had no marked effects(P>0.05)on the growth performance of pigs.However,pigs receiving EO diet had higher(P<0.05)serumglutathione peroxidase(GSH-Px)activity,and tended to decrease(P=0.063)serum concentration of tumor necrosis factor-a(TNF-a).In addition,pigs receiving EO diet had higher(P<0.05)abundances of phylum Actinobacteria,and genera Bifidobacterium,and lower(P<0.05)phylum Bacteroidetes and genera Alloprevotella in colonic digesta.Pigs receiving PRO diet decreased(P<0.05)the serum concentration of malondialdehyde(MDA)and diamine oxidase activity,increased(P<0.05)the villus height and the ratio of villus height to crypt depth in duodenum,increased sucrase activity in jejunal mucosa,and also increased the abundance of phylum Actinobacteria in colonic digesta.Furthermore,the synergistic effects of EO and PROwas observed(P<0.05)for pigs with decreasing serum TNF-a concentration and increasing serum GSH-Px activity.Collectively,the results indicated that dietary supplementation of EO and PRO had no significant effects on growth performance of weaned pigs.EO diet appeared to improve antioxidant activity and intestinal microbiota,while PRO diet improved intestinal morphology and digestive enzyme activity,and there was a synergistic effect of EO and PRO on reducing inflammatory parameters in weaned pigs. | Xie Peng Qiang Zhou Cheng Wu Jian Zhao Quan Tan Ying He Liang Hu Zhengfeng Fang Yan Lin Shengyu Xu Bin Feng Jian Li Yong Zhuo Chris Van Ginneken Xuemei Jiang De Wu Lianqiang Che | 2022 | Animal Nutrition2022，，2： | 8 |
| 4 | The Glutamatergic Postrhinal Cortex–Ventrolateral Orbitofrontal Cortex Pathway Regulates Spatial Memory Retrieval显示文摘A deficit in spatial memory has been taken as an early predictor of Alzheimer’s disease(AD) or mild cognitive impairment(MCI). The uncinate fasciculus(UF) is a long-range white-matter tract that connects the anterior temporal lobe with the orbitofrontal cortex(OFC)in primates. Previous studies have shown that the UF impairment associated with spatial memory deficits may be an important pathological change in aging and AD, but its exact role in spatial memory is not well understood. The pathway arising from the postrhinal cortex(POR) and projecting to the ventrolateral orbitofrontal cortex(vlOFC)performs most of the functions of the UF in rodents.Although the literature suggests an association between spatial memory and the regions connected by the POR–vlOFC pathway, the function of the pathway in spatialmemory is relatively unknown. To further illuminate the function of the UF in spatial memory, we dissected the POR–vlOFC pathway in mice. We determined that the POR–vlOFC pathway is a glutamatergic structure, and that glutamatergic neurons in the POR regulate spatial memory retrieval. We also demonstrated that the POR–vlOFC pathway specifically transmits spatial information to participate in memory retrieval. These findings provide a deeper understanding of UF function and dysfunction related to disorders of memory, as in MCI and AD. | Xinyang Qi Zhanhong Jeff Du Lin Zhu Xuemei Liu Hua Xu Zheng Zhou Cheng Zhong Shijiang Li Liping Wang Zhijun Zhang | 2019 | Neuroscience Bulletin2019，35，3： | 7 |
| 5 | Fast-Suppressor Screening for New Components in Protein Trafficking,Organelle Biogenesis and Silencing Pathway in Arabidopsis thaliana Using DEX-Inducible FREE1-RNAi Plants显示文摘Membrane trafficking is essential for plant growth and responses to external signals.The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex(endosomal sorting complex required for transport).FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body(MVB),vacuolar protein transport and vacuole biogenesis,and autophagic degradation.FREE1 knockout plants show defective MVB formation,abnormal vacuolar transport,fragmented vacuoles,accumulated autophagosomes,and seedling lethality.To further uncover the underlying mechanisms of FREE1 function in plants,we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants.The obtained mutants are termed as suppressors of free1(sof).To date,229 putative sof mutants have been identified.Barely detecting of FREE1 protein with M3plants further identified 84 FREE1-related suppressors.Also145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants.Through next-generation sequencing(NGS)of bulked DNA from F2mapping population of two RNAi-related sof mutants,FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly.These FREE1-and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis,as well as for uncovering the new components involved in the regulation of silencing pathways in plants. | Qiong Zhao Caiji Gao PoShing Lee Lin Liu Shaofang Li Tangjin Hu Jinbo Shen Shuying Pan Hao Ye Yunru Chen Wenhan Cao Yong Cui Peng Zeng Sheng Yu Yangbin Gao Liang Chen Beixin Mo Xin Liu Shi Xiao Yunde Zhao Silin Zhong Xuemei Chen Liwen Jiang | 2015 | Journal of Genetics and Genomics2015，42，6： | 4 |
| 6 | Investigation of microstructure changes in Al_(2)O_(3)-YSZ coatings and YSZ coatings and their effect on thermal cycle life显示文摘Yttria-stabilized zirconia(YSZ)coatings and Al_(2)O_(3)–YSZ coatings were prepared by atmospheric plasma spraying(APS).Their microstructural changes during thermal cycling were investigated via scanning electron microscopy(SEM)equipped with electron backscatter diffraction(EBSD)and X-ray diffraction(XRD).It was found that the microstructure and microstructure changes of the two coatings were different,including crystallinity,grain orientation,phase,and phase transition.These differences are closely related to the thermal cycle life of the coatings.There is a relationship between crystallinity and crack size.Changes in grain orientation are related to microscopic strain and cracks.Phase transition is the direct cause of coating failure.In this study,the relationship between the changes in the coating microstructure and the thermal cycle life is discussed in detail.The failure mechanism of the coating was comprehensively analyzed from a microscopic perspective. | Meiqi DAI Xuemei SONG Chucheng LIN Ziwei LIU Wei ZHENG Yi ZENG | 2022 | Journal of Advanced Ceramics2022，11，2： | 4 |
| 7 | RNA Quality Control as a Key to Suppressing R Silencing of Endogenous Genes in Plants显示文摘内长的 RNA 的 RNA 质量控制是真核细胞的基因表示的不可分的部分并且经常依靠 exonucleolytic 降级消除不正常的抄本。在平行,外长、选择的内长的 RNA 通过 RNA silencing 被降级,它是许多优核质使用的染色体防卫机制。在植物, RNA silencing 被 RNA 依赖的 RNA 聚合酶(RDR ) 被双 stranded RNA (dsRNAs ) 的生产触发并且通过小介入继续(si )指导 RNA, ARGONAUTE (以前) 相应抄本的调停的劈开。许多研究表明植物由把 RNA 监视机制用作一项保护措施保护他们的 transcriptome 侧面避免内长的编码基因的不恰当的 posttranscriptional 基因 silencing。在监视和 RNA silencing 保证在这些降级小径之中的内长的 RNA 底层的适当划分的 RNA 之间的战争的拖船。这里,我们在内长的基因在 RNA silencing 的抑制在 RNA 质量控制和它的角色上考察最近的进展并且讨论在这些小径之中位于串音下面的机制。 | Lin Liu Xuemei Chen | 2016 | Molecular Plant2016，9，6： | 3 |
| 8 | Fluorescence enhancement of Tb^(3+) complexes by adding silica-coated silver nanoparticles显示文摘Six kinds of terbium ternary complexes with halo-benzoic acids were synthesized. Their compositions were determined by C, H elemental analyzer and EDTA titration. The infrared spectra, ultraviolet absorption spectra, and fluorescence spectra were also measured to identify the complexes. Elemental analysis showed that the compositions of these complexes were Tb(p-Br BA)3?H2O, Tb(p-Cl BA)3?2H2O, Tb(p-FBA)3?H2O, Tb(o-FBA)3?2H2O, Tb(o-Cl BA)3?H2O, and Tb(o-Br BA)3?H2O, respectively. The monodispersed Ag@Si O2 core-shell nanoparticles with silica thicknesses of 10, 15, and 25 nm were successfully prepared and characterized by transmission-electron microscopy. Fluorescence intensities of the complexes were detected before and after Ag@Si O2 core-shell nanoparticles were added; the enhancement times were related to the silica-shell thickness. The fluorescence enhancement times were largest when the thickness of the silica shell was 25 nm. The mechanism may be attributed to the localized surface-plasmon resonance. Furthermore, the enhancement effect of terbium fluoro-benzoate complexes was the strongest in these complexes. This result may be attributed to the hydrogen bond between the hydroxyl on the surface of the silica shell and the fluorine atom. | Dan Zhou Xuemei Lin Ailing Wang Jingjing Li Yanrong Qu Haibin Chu Yongliang Zhao | 2015 | Science China Chemistry2015，58，6： | 2 |
| 9 | Impaired Parahippocampal Gyrus-Orbitofrontal Cortex Circuit Associated with Visuospatial Memory Deficit as a Potential Biomarker and Interventional Approach for Alzheimer Disease显示文摘The parahippocampal gyrus-orbitofrontal cortex(PHG-OFC)circuit in humans is homologous to the postrhinal cortex(POR)-ventral lateral orbitofrontal cortex(vlOFC)circuit in rodents.Both are associated with visuospatial malfunctions in Alzheimer’s disease(AD).However,the underlying mechanisms remain to be elucidated.In this study,we explored the relationship between an impaired POR-vlOFC circuit and visuospatial memory deficits through retrograde tracing and in vivo local field potential recordings in 5XFAD mice,and investigated alterations of the PHG-OFC circuit by multi-domain magnetic resonance imaging(MRI)in patients on the AD spectrum.We demonstrated that an impaired glutamatergic POR-vlOFC circuit resulted in deficient visuospatial memory in 5XFAD mice.Moreover,MRI measurements of the PHG-OFC circuit had an accuracy of 77.33%for the classification of amnestic mild cognitive impairment converters versus non-converters.Thus,the PHG-OFC circuit explains the neuroanatomical basis of visuospatial memory deficits in AD,thereby providing a potential predictor for AD progression and a promising interventional approach for AD. | Lin Zhu Zan Wang Zhanhong Du Xinyang Qi Hao Shu Duan Liu Fan Su Qing Ye Xuemei Liu Zheng Zhou Yongqiang Tang Ru Song Xiaobin Wang Li Lin Shijiang Li Ying Han Liping Wang Zhijun Zhang | 2020 | Neuroscience Bulletin2020，36，8： | 2 |
| 10 | Advances in Urban Meteorological Research in China显示文摘Over the past decades,a large number of studies have been carried out in the field of urban meteorology in China.This paper summarizes the main progress in urban meteorology research from four aspects:urban meteorological observation network and field campaign,multi-scale model of urban meteorology,interaction between urban meteorology and atmospheric environment,and the impacts of urbanization on weather and climate.Major advances are as follows.China’s major cities have established or are improving comprehensive urban meteorological observation networks characterized by multi-platform,multi-variable,multi-scale,multi-link,and multi-function.Beijing,Nanjing,Shanghai,and other cities carried out urban meteorological field campaigns,which were included in the WMO research demonstration project.Wind tunnel experiments and scale-model outdoor experiments were successfully conducted.Multi-scale urban meteorological and air quality prediction numerical model systems have been developed and put into operational use.The urban heat island effect;urban impacts on precipitation,regional climate,and air quality;urban planning;and interaction between urban meteorology and atmospheric environment are extensively investigated.Finally,efforts to improve observational technology,data assimilation,and urban system modeling,to explore the impacts of urbanization on environment and human health,and to provide integrated urban hydro-meteorological climate and environmental services are planned ahead. | Shiguang MIAO Weimei JIANG Ping LIANG Hongnian LIU Xuemei WANG Jianguo TAN Ning ZHANG Ju LI Wupeng DU Lin PEI | 2020 | Journal of Meteorological Research2020，34，2： | 2 |
| 11 | The dysregulated autophagy signaling is partially responsible fordefective podocyte development in wt1a mutant zebrafish显示文摘Podocytes are essential elements of the glomerular filtration barrier and relevant to many kidney diseases.But the molecular mechanism in podocyte development is still unclear.Wilms tumor protein(WT1)is an essential factor for urogenital system development.Zebrafish have two wt1 genes,naming wt1a and wt1b.Here,we examined the roles of both wt1 genes in zebrafish embryonic development employing the CRISPR/Cas9 technology.The homozygous wt1a mutants displayed developmental malformations including pericardial edema,yolk sac edema and failure of glomerulus development.Loss of wt1a function disrupted podocyte differentiation and inhibited expression of podocin and nephrin.In contrast,the homozygous wt1b mutants were phenotypically normal and had no severe phenotypes of nephrogenesis defects that were observed in wt1b morpholino knockdown embryos.Therefore,wt1a plays an essential role in kidney development,whereas wt1b might not be essential for kidney development.Interestingly,from previous studies we knew that autophagy is involved in podocyte differentiation.By detecting the protein level of P62 and LC3A/B,we found the autophagy signal was greatly reduced in wt1a mutants.To better understand the relationship between autophagy signal and wt1a function in podocyte development,rapamycin was added to activate autophagy in wt1a-mutated embryos.As a result,podocyte injury was ameliorated in wt1a homozygous mutants by rapamycin treatment.In conclusion,our findings not only confirmed the essential function of wt1a during kidney development,but also provided a possible way for treatment of diseases involving podocyte injury. | Xuemei Zhang Qiaohong Lin Fan Ren Jin Zhang Farman Ullah Dawar Jie Mei | 2018 | Aquaculture and Fisheries2018，3，3： | 2 |
| 12 | Development of Nucleic Acid Sequence-Based Amplification Assay for Detection of Macrobrachium rosenbergii Nodavirus显示文摘A nucleic acid sequence-based amplification(NASBA)assay was established for the detection of Macrobrachium rosenbergii Nodavirus(MrNV).The specific primers were designed according to the high conserved region of RNA2 sequence of MrNV.The 224 bp specific amplification product was obtained in positive sample determined with 3%agarose gel electrophoresis,while no product was generated from shrimp infected with other viruses including DNA viruses(IHHNV,WSSV)and RNA viruses(TSV,IMNV,YHV).The detecting limit of the assay was 8pg nucleic acid,which is more sensitive than that of PCR method. | Feng LIN Li LIU Dong QIAN Guijie HAO Pengcheng SHENG Zheng CAO Xuemei YUAN Jinyu SHEN | 2014 | Agricultural Biotechnology2014，3，3： | 2 |
| 13 | Identification of Functional Genetic Variants in Cyclooxygenase-2 and Their Association With Risk of Esophageal Cancer显示文摘 | Xuemei Zhang Xiaoping Miao Wen Tan Baitang Ning Zhihua Liu Yuan Hong Wenguang Song Yongli Guo Xinyu Zhang Yan Shen Boqin Qiang Fred F. Kadlubar Dongxin Lin | 2005 | Gastroenterology2005，，2： | 1 |
| 14 | Structural isomer and high-yield of Pt1Ag28 nanoduster via one-pot chemical wet method显示文摘In order to understand the structure-property correlation and explore the application of metal nanoclusters, it is important and intriguing to determine their crystal structure and obtain high-yield. At the same time, this is also a challenge in nanoscience and technology. Here, we report the highly efficient synthesis of Pt1Ag28 nanocluster via one-pot chemical wet method. The crystal structure of Pt1Ag28 nanocluster was determined by X-ray crystallography to be a face centered cubic (FCC) kernel. This novel structure is the structural isomerization of Pt1Ag28 nanocluster reported before. This phenomenon is first discovered in the synthesis of alloy nanoclusters. In addition, Pt1Ag28 nanocluster has high yield and exhibits potential optics in the near infrared (NIR) fluorescent imaging. The time-dependent density functional theory (TD-DFT) calculation implied that the optical property of Pt1Ag28 was sensitive to its structure. This work provides a simple method to synthesize alloy nanoclusters with structural isomerization. | Xinzhang Lin Chao Liu Keju Sun Ren'an Wu Xuemei Fu Jiahui Huang | 2019 | Nano Research2019，12，2： | 1 |
| 15 | Identification of Functional Genetic Variants in Cyclooxygenase-2 and Their Association With Risk of Esophageal Cancer显示文摘 | Xuemei Zhang Xiaoping Miao Wen Tan Baitang Ning Zhihua Liu Yuan Hong Wenguang Song Yongli Guo Xinyu Zhang Yan Shen Boqin Qiang Fred F. Kadlubar Dongxin Lin | 2005 | Gastroenterology2005，，2： | 1 |
| 16 | Identification of Functional Genetic Variants in Cyclooxygenase-2 and Their Association With Risk of Esophageal Cancer显示文摘 | Xuemei Zhang Xiaoping Miao Wen Tan Baitang Ning Zhihua Liu Yuan Hong Wenguang Song Yongli Guo Xinyu Zhang Yan Shen Boqin Qiang Fred F. Kadlubar Dongxin Lin | 2005 | Gastroenterology2005，，2： | 1 |
| 17 | An Integrated “Energy Wire” for both Photoelectric Conversion and Energy Storage显示文摘 | Tao Chen Longbin Qiu Zhibin Yang Zhenbo Cai Jing Ren Houpu Li Huijuan Lin Xuemei Sun Huisheng Peng | 2012 | Angew Chem Int Ed2012，，48： | 1 |
| 18 | Entrainment of Intestinal Slow Waves with Electrical Stimulation Using Intraluminal Electrodes显示文摘 | Xuemei Lin J. Hayes L. J. Peters J. D. Z. Chen | 2000 | Annals of Biomedical Engineering2000，，5： | 1 |
| 19 | Dendroclimatic evaluation of climate-growth relationships of Meyer spruce (Picea meyeri) on a sandy substrate in semi-arid grassland, north China显示文摘 | Eryuan Liang Xuemei Shao Yuxi Hu Jinxing Lin | 2001 | Trees2001，，4： | 1 |
| 20 | Characterization of Gastric Myoelectrical Rhythms in Patients with Systemic Sclerosis Using Multichannel Surface Electrogastrography显示文摘 | Terry McNearney Xuemei Lin Jharana Shrestha Jeffrey Lisse J.D.Z. Chen | 2002 | Digestive Diseases and Sciences2002，，4： | 1 |
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