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| 1 | XA23 Is an Executor R Protein and Confers Broad-Spectrum Disease Resistance in Rice显示文摘 | Chunlian Wang Xiaoping Zhang Yinglun Fan Ying Gao Qinlong Zhu Chongke Zheng Tengfei Qin Yanqiang Li Jinying Che Mingwei Zhang Bing Yang Yaoguang Liu Kaijun Zhao | 2015 | Molecular Plant2015，8，2： | 55 |
| 2 | HrcQ is necessary for Xanthomonas oryzae pv. oryzae HR-induction in non-host tobacco and pathogenicity in host rice显示文摘Bacterial blight, caused by Xanthomonas oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice(Oryza sativa L.) worldwide. The type III secretion system(T3SS) of Xoo, encoded by the hrp(hypersensitive response and pathogenicity) genes, plays critical roles in conferring pathogenicity in host rice and triggering a hypersensitive response(HR) in non-host plants. To investigate the major genes conferring the pathogenicity and avirulence of Xoo, we previously constructed a random Tn5-insertion mutant library of Xoo strain PXO99A. We report here the isolation and characterization of a Tn5-insertion mutant PXM69. Tn5-insertion mutants were screened on indica rice JG30, which is highly susceptible to PXO99A, by leaf-cutting inoculation.Four mutants with reduced virulence were obtained after two rounds of screening. Among them, the mutant PXM69 had completely lost virulence to the rice host and ability to elicit HR in non-host tobacco. Southern blotting analysis showed a single copy of a Tn5-insertion in the genome of PXM69. PCR walking and sequencing analysis revealed that the Tn5 transposon was inserted at nucleotide position 70,192–70,201 in the genome of PXO99A, disrupting the type III hrc(hrp-conserved) gene hrcQ, the first gene in the D operon of the hrp cluster in Xoo. To confirm the relationship between the Tn5-insertion and the avirulence phenotype of PXM69, we used the marker exchange mutagenesis to create a PXO99Amutant, ΔhrcQ::KAN, in which the hrcQ was disrupted by a kanamycin-encoding gene cassette at the same site as that of the Tn5-insertion. ΔhrcQ::KAN showed the same phenotype as mutant PXM69. Reintroduction of the wild-type hrcQ gene partially complemented the pathogenic function of PXM69. RT-PCR and cellulase secretion assays showed that the Tn5-disruption of hrcQ did not affect transcription of downstream genes in the D operon and function of the type II secretion system. Our results provide new insights into the pathogenic functions of clustered hrp genes in Xoo. | Xiaoping Zhang Chunlian Wang Chongke Zheng Jinying Che Yanqiang Li Kaijun Zhao | 2013 | The Crop Journal2013，1，2： | 4 |
| 3 | Testing protocols in Internet of Things by a formal passive technique显示文摘In recent years,Extensible Messaging and Presence Protocol(XMPP)is gaining momentum in Internet of Things(IoT).It has been widely used in chatting,message exchanging and unique addressing.As a matter of course,it raises an interesting issue:how to formally test the conformance and performance of XMPP in IoT environment.While conformance testing of communicating protocols is a functional test that verifies whether the behaviors of the protocol satisfy defined requirements,performance testing is a qualitative and quantitative test that aims at checking whether the performance requirements of the protocol are satisfied under certain conditions.In this paper,we present a logic-based passive testing approach that can test both the conformance and the performance of XMPP protocol through real execution traces and formally specified properties.To evaluate and assess our methodology,we present a developed prototype and the experiments with a set of XMPP properties.Finally,the relevant verdicts and conclusions are provided. | Xiaoping CHE Stephane MAAG | 2014 | Science China(Information Sciences)2014，57，3： | 4 |
| 4 | Design of Characteristic Curriculum on Software Engineering Major for Undergraduate-Graduate Education显示文摘The concept of“New Engineering”has put forward new challenges to the talents cultivation of universities.Due to some problems of the traditional Software Engineering curriculum,e.g.separated design at undergraduate-level and graduate-level courses,poor curriculum structure,lacking of industry characteristics.This paper proposes an integrated undergraduate-graduate education curriculum for Software Engineering Major,which is based on Software Engineering specialty knowledge system(C-SWEBOK)and focuses on the current national strategic demands.Additionally,the curriculum combines with the University’s transportation characteristics,and fuses the discipline of Software Engineering and Intelligent Transportation.The multi-level curriculum designed in this paper is with reasonable structure,complete system,progressive content,and salient feature,which provides the strong support for cultivating high-qualified software talents in line with national strategies and industry needs. | Weiwei Xing Peng Bao Xiaoping Che Di Zhang Lingyun Lu Wei Lu | 2021 | 计算机教育2021，，12： | 0 |
| 5 | Inhibition of ammonia-oxidizing bacteria promotes the growth of ammonia-oxidizing archaea in ammonium-rich alkaline soils显示文摘Disparities in the substrate affinity and tolerance threshold for ammonia have been believed to play a key role in driving niche differentiation between ammonia-oxidizing archaea (AOA) and bacteria (AOB);however, recent surveys argue that direct competition between AOA and AOB is also important in this phenomenon. Accordingly, it is reasonable to predict that diverse AOA lineages would grow in ammonium (NH_(4)^(+))-rich alkaline arable soils if AOB growth is suppressed. To test this hypothesis, a microcosm study was established using three different types of alkaline arable soils, in which a high NH_(4)^(+) concentration (200 μg N g^(-1) dry soil) was maintained by routinely replenishing urea and the activities of AOB were selectively inhibited by 1-octyne or 3,4-dimethylpyrazole phosphate (DMPP). Compared with amendment with urea alone, 1-octyne partially retarded AOB growth, while DMPP completely inhibited AOB. Both inhibitors accelerated the growth of AOA, with significantly higher ratios of abundance of AOA to AOB observed with DMPP amendment across soils. Nonmetric multidimensional scaling analysis (NMDS) indicated that different treatments significantly altered the community structures of both AOA and AOB and AOA OTUs enriched by high-NH_(4)^(+) amendment were taxonomically constrained across the soils tested and closely related to Nitrososphaera viennensis EN76 and N. garnensis. Given that these representative strains have been demonstrated to be sensitive to high ammonia concentrations, our results suggest that it is the competitiveness for ammonia, rather than disparities in substrate affinity and tolerance threshold for ammonia, that drives niche differentiation between these phylotypes and AOB in NH_(4)^(+)-rich alkaline soils. | Chang YIN Xiaoping FAN Hao CHEN Mujun YE Guochao YAN Tingqiang LI Hongyun PENG Shengzhe E Zongxian CHE Steven AWAKELIN Yongchao LIANG | 2022 | Pedosphere2022，32，4： | 0 |
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