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| 1 | Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood,bone marrow and peritoneal lavage of pancreatic carcinoma patients显示文摘AIM: To evaluate the diagnostic potential of cytokeratin-19 (CK-19) mRNA for the detection of disseminated tumor cells in blood, bone marrow and peritoneal lavage in patients with ductal adenocarcinoma of the pancreas. METHODS: Sixty-eight patients with pancreatic cancer (n = 37), chronic pancreatitis (n = 16), and non-pan- creatic benign surgical diseases (n = 15, control group) were included in the study. Venous blood was taken preoperatively, intraoperatively and at postoperative d 1 and 10. Preoperative bone marrow aspirates and peritoneal lavage taken before mobilization of the tumor were analyzed. All samples were evaluated for disseminated tumor cells by CK-19-specific nested-PCR and quantitative fluorogenic RT-PCR. RESULTS: CK-19 mRNA expression was increased in 24 (64%) blood samples and 11 (30%) of the peritoneal lavage samples in the patients with pancreatic cancer. In 15 (40%) of the patients with pancreatic cancer, disseminated tumor cells were detected in venous blood and bone marrow and/or peritoneal lavage. In the peritoneal lavage, the detection rates were correlated with the tumor size and the tumor differentiation. CK-19 levels were increased in pT3/T4 and moderately/poorly differentiated tumors (G2/G3). Pancreatic cancer patients with at least one CK-19 mRNA-positive sample showed a trend towards shorter survival. Pancreatic cancerpatients showed significantly increased detection rates of disseminated tumor cells in blood and peritoneal lavage compared to the controls and the patients with chronic pancreatitis. CONCLUSION: Disseminated tumor cells can be detected in patients with pancreatic ductal adenocar- cinoma by CK-19 fluorogenic RT-PCR. In peritoneal lavage, detection rate is correlated with tumor stage and differentiation. In the clinical use, CK-19 is suitable for the distinction between malignant and benign pancreatic disease in combination with other tumor-specific markers. | Katrin Hoffmann Christiane Kerner Wolfgang Wilfert Marc Mueller Joachim Thiery Johann Hauss Helmut Witzigmann | 2007 | World Journal of Gastroenterology2007，13，2： | 20 |
| 2 | Inhibition of histone deacetylase for the treatment of biliary tract cancer:A new effective pharmacological approach显示文摘瞄准:调查在试管内和在活体内治疗学的效果嘘胆道癌症上的一 deacetylase 禁止者 NVP-LAQ824 和 NVP-LBH589。方法:由 NVP-LAQ824 和 NVP-LBH589 的房间生长抑制是在由 MTT 试金的 7 根人的胆道癌症房间线的学习在试管内。另外, NVP-LBH589 的 anti-tumoral 效果在一个妄想的老鼠模型被学习。Anti-tumoral 药机制被为 MIB-1 为 acH4 和 p21 弄污 WAF-1/CIP-1, PARP 试金,房间周期分析, TUNEL 试金,和 immunhistochemistry 的免疫估计。结果:有两混合物的在试管内治疗显著地压制了所有癌症房间线的生长[吝啬的 IC50 ( 3 d ) 0.11 和 0.05 mumol/L ,分别地],并且与 nucleosomal 的 hyperacetylation 被联系嘘一 H4 , p21 WAF-1/CIP-1 的增加的表示, apoptosis ( PARP 劈开)感应,并且房间周期在 G2/M 逮捕检查点。在 28 d 以后, NVP-LBH589 显著地减少了在 66% 集体的肿瘤(胆汁管癌) 并且 87%( 胆囊癌症) 与安慰剂相比的在活体内,并且加强 gemcitabine 的功效。揭示的肿瘤标本的进一步的分析由 TUNEL 试金增加了 apoptosis 并且减少了房间增长(MIB-1 ) 。结论:我们的调查结果建议 NVP-LBH589 和 NVP-LAQ824 对人的胆道癌症是活跃的在试管内。另外, NVP-LBH589 表明了重要在活体内活动并且加强 gemcitabine 的功效。因此,为胆道癌症的治疗的这新药的进一步临床的评估被推荐。 | Thilo Bluethner Manuel Niederhagen Karel Caca Frederik Serr Helmut Witzigmann Christian Moebius Joachim Mossner Marcus Wiedmann | 2007 | World Journal of Gastroenterology2007，13，35： | 5 |
| 3 | Prospective evaluation of circulating hepatocytes by alpha-fetoprotein messenger RNA in patients with hepatocellular carcinoma显示文摘 | Witzigmann H Geissler F Benedix F | 2002 | Surgery2002，131，1： | 1 |
| 4 | Prospective evalu ation of circulating hepatocytes by alpha-fetoprotein messen ger RNA in patients with hepatocellular carcinoma 显示文摘 | Witzigmann H Geissler F Benedix F et ai | 2002 | surgery2002，131，1： | 1 |
| 5 | Endothelin (a) re- ceptor blockade reduces ischemia/reperfusion injury in pig pancreas transplantation显示文摘 | Witzigmann H Ludwig S Armann B | 2003 | Ann Surg2003，238，2： | 1 |
| 6 | Prospective evaluation of circulating hepatocytes by alpha-fetoprotein messenger RNA in patients with hepatocetlular carcinoma 显示文摘 | Witzigmann H Geissler F Benedix F | 2002 | Surgery2002，131，1： | 1 |
| 7 | Outcome after duodenum-preserving pancreatic head resection is improved compared with classic Whipple procedure in the treatment of chronic pancreatitis显示文摘 | Witzigmann H Max D Uhlmann Vf | 2003 | Surger-y2003，134，： | 1 |
| 8 | Management and outcome in patients with Klatskin-mimicking lesions of the biliary tree显示文摘 | Dirk Uhlmann M.D. Marcus Wiedmann M.D. Frank Schmidt M.D. Regine Kluge M.D. Andrea Tannapfel M.D. Frieder Berr M.D. Johann Hauss M.D. Helmut Witzigmann M.D | 2006 | Journal of Gastrointestinal Surgery2006，，8： | 1 |
| 9 | Nano-medicine in cancer therapy : Challenges,opportunities,andclinical applications 显示文摘 | Wickki A Witzigmann D Balasubramanian V | 2015 | J Control Release2015，200，： | 1 |
| 10 | Splenohepatic arterial steal syndrome in liver transplantalion: clinical features and management显示文摘 | Geissler I Lamesch P Witzigmann H | 2002 | Transpl Int2002，15，： | 1 |
| 11 | Endothelin(A) receptor blockade reduces isehemia/reperfusion injury in pig pancreas transplantation显示文摘 | Witzigmann H Ludwig S Armann B | 2003 | Ann Surg2003，238，2： | 1 |
| 12 | Splenohepatic arterial steal syndrome in liver transplantation:clinical features and management显示文摘 | Geissler I Lamesch P Witzigmann H | 2002 | Transpl Int2002，15，： | 1 |
| 13 | Splenohepatic arterial steal syndrome in liver transplantation: clinical features and management显示文摘 | Ina Geissler Peter Lamesch Helmut Witzigmann Uwe Jost Johann Hauss Josef Fangmann | 2002 | Transplant International (-)2002，，2： | 1 |
| 14 | Prospective evaluation of circulating hepatocytes by alpha-fetoprotein messenger RNA in patients with hepatocellular carcinoma显示文摘 | Witzigmann H Geissler F Benedix F | 2002 | Surgery2002，131，1： | 1 |
| 15 | Dispersion, wave propagation and efficiency analysis of nanowire solar cells 显示文摘 | J Kupec B Witzigmann | 2009 | Opt Express2009，17，10： | 1 |
| 16 | Surgical and palliative mangement and outcome in 184 patients with hihr cholangiocarcinoma:palliative photodynamic therapy plus stenting is comparable to rl/r2 resection 显示文摘 | Witzigmann H Bert F MD Ringel U | 2006 | Annals of Surgery2006，244，2： | 1 |
| 17 | Prospective evaluation of circulating hepatocytes by alpha-fetoprotein messenger RNA in patients with hepatocellular carcinoma 显示文摘 | Witzigmann H Geissler F Benedix F | 2002 | Surgery2002，131，1： | 1 |
| 18 | Current diagnostics and therapy for carcinomas of the biliary tree and gallbladder 显示文摘 | Wiedmann M Schoppmeyer K Witzigmann H | 2005 | Zeitschrift Gastroenterol2005，43，5： | 1 |
| 19 | Intra - abdominal abscess显示文摘 | Witzigmann H Geissler F Uhlmnn D | 1998 | Chirurg1998，69，8： | 1 |
| 20 | The ultrasound scalpel in laparoscopic surgery显示文摘 | Witzigmann H Otto M Hauss J | 1996 | Chimrg1996，67，4： | 1 |
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