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| 1 | Inositol Pyrophosphate lnsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis显示文摘The maintenance of cellular phosphate(Pi)homeostasis is of great importance in living organisms.The SPX domain-containing protein 1(SPX1)proteins from both Arabidopsis and rice have been proposed to act as sensors of Pi status.The molecular signal indicating the cellular Pi status and regulating Pi homeostasis in plants,however,remains to be identified,as Pi itself does not bind to the SPX domain.Here,we report the identification of the inositol pyrophosphate lnsP8 as a signaling molecule that regulates Pi homeostasis in Arabidopsis.Polyacrylamide gel electrophoresis profiling of InsPs revealed that lnsP8 level positively correlates with cellular Pi concentration.We demonstrated that the homologs of diphosphoinositol pentaki-sphosphate kinase(PPIP5K),VIH1 and VIH2,function redundantly to synthesize lnsP8,and that the vih1 vih2 double mutant overaccumulates Pi.SPX1 directly interacts with PHR1,the central regulator of Pi starvation responses,to inhibit its function under Pi-replete conditions.However,this interaction is compromised in the vih1 vih2 double mutant,resulting in the constitutive induction of Pi starvation-induced genes,indicating that plant cells cannot sense cellular Pi status without lnsP8.Furthermore,we showed that lnsP8 could directly bind to the SPX domain of SPX1 and is essential for the interaction between SPX1 and PHR1.Collectively,our study suggests that lnsP8 is the intracellular Pi signaling molecule serving as the ligand of SPX1 for controlling Pi homeostasis in plants. | Jinsong Dong Guojie Ma Liqian Sui Mengwei Wei Viswanathan Satheesh Ruyue Zhang Shenghong Ge Jinkai Li Tong-En Zhang Christopher Wittwer Henning JJessen Huiming Zhang Guo-Yong An Dai-Yin Chao Dong Liu Mingguang Lei | 2019 | Molecular Plant2019，12，11： | 8 |
| 2 | Fluorescence monitoring of rapid cycle PCR for quantification 显示文摘 | Wittwer CT Rirle KM Rassmussen RP | 1998 | Gene Quantification1998，129，： | 2 |
| 3 | High-resolution DNA melting analysis in clinical research and diagnostics显示文摘 | Montgomery JL Sanford LN Wittwer CT | 2010 | Expert Rev Mol Diagn2010，10，2： | 1 |
| 4 | Fluorescein-labeled oligonucleotides for real-time PCR:Using the inherent quenching of deoxyguanosine nucleotides显示文摘 | Crockett A O Wittwer C T | 2001 | Anal Biochem2001，290，1： | 1 |
| 5 | Continuous fluorescence monitoring of rapid cycle DNA amplification 显示文摘 | Wittwer CT Herrmann MG Moss AA | 1997 | Biotechniques1997，22，1： | 1 |
| 6 | Fluctuating and mean pressure covering in wind tunnel ts on a stadium 显示文摘 | Marighetti J Wittwer A De Bortoli M | 2000 | Journal of Wind Engineering and Industrial Aerodynamics2000，84，3： | 1 |
| 7 | A comparison of high-resolution melting analysis with denaturing high-performance liquid chromatography for mutation scanning:cystic fibrosis transmembrane conductance regulator genc as a model显示文摘 | Chou LS Lyon E Wittwer CT | 2005 | Am J Clin Pathol2005，124，3： | 1 |
| 8 | Celsior solution for improvement of currently used clinical standards of lung preservation in an ex vivo rat model显示文摘 | Thorsten Wittwer Thorsten Wahlers Jan F Cornelius | 1999 | European Journal of Cardio-thoracic Surgery1999，15，： | 1 |
| 9 | Continu ous fluorescence monitoring of rapid cycle DNA amplifica tion显示文摘 | Wittwer C T Herrmann M G Moss A A | | 0，，01： | 1 |
| 10 | The Direct Linearization Method Applied to Position Error in Kinematic Linkages显示文摘 | WITTWER J W CHASE K W HOWELL L L | 2004 | Mechanism and Machine Theory2004，39，7： | 1 |
| 11 | High-resolution genotyping by amplicon melting analysis using LCGreen显示文摘 | Wittwer CT Reed GH Gundry CN | 2003 | Clin Chem2003，49，61： | 1 |
| 12 | Simultaneous mutation scanning and genotyping by high-resolution DNA melting analysis显示文摘 | Montgomery J Wittwer C T Palais R | 2007 | Nat Protoc2007，2，1： | 1 |
| 13 | High-resolution DNA melting analysis:advan-cements and limitations显示文摘 | Wittwer CT | 2009 | Hum Mutat2009，30，6： | 1 |
| 14 | Cross-competition for binding of alpha 1-antitrypsin (alpha 1 AT)-elastase complexes to the serpin-enzyme complex receptor by other serpin-enzyme complexes and by proteolytically modified alpha 1 AT显示文摘 | Joslin G Wittwer A Adams S | 1993 | Journal of Biological Chemistry1993，268，3： | 1 |
| 15 | Continuous fluorescence monitoring of rapid cycle DNA amplification显示文摘 | WITTWER C T HERRMANN M G MOSS A A | 1997 | Biotechniques1997，22，： | 1 |
| 16 | High-resolution genotyping by amplicon melting analysis using LCGreen显示文摘 | Wittwer C T Reed G H Gundry C N | 2003 | Clin Chem2003，49，： | 1 |
| 17 | High-resolution DNA melting analysis for simple and efficient molecular diagnostics 显示文摘 | Reed GH Kent JO Wittwer CT | 2007 | Pharmacogenomics2007，8，6： | 1 |
| 18 | Complications after zygoma fracture fixation: Is there a difference between biodegradable materials and how do they compare with titanium osteosynthesis?显示文摘 | Gert Wittwer Wasiu Lanre Adeyemo Kaan Yerit Martin Voracek Dritan Turhani Franz Watzinger Georg Enislidis | 2006 | Oral Surgery Oral Medicine Oral Pathology Oral Radiology and Endodontology2006，，4： | 1 |
| 19 | High-resolution genotyping by amplieon melting analysis using LC Green显示文摘 | Wittwer CT Reed GH Gundry CN | 2003 | Clin Chem2003，49，6： | 1 |
| 20 | A complete proof of the Feigenbaum conjectures显示文摘 | Jean-Pierre Eckmann Peter Wittwer | 1987 | Journal of Statistical Physics (-)1987，，3： | 1 |
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