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| 1 | Dietary supplementation with Clostridium butyricum improves growth performance of broilers by regulating intestinal microbiota and mucosal epithelial cells显示文摘Clostridium butyricum has been widely considered an antibiotic substitute in recent years.It can promote growth performance,improve the immune response and enhance the intestinal barrier function of the host.In the present study,1-d-old Arbor Acres(AA)broilers were fed C.butyricum(1×109 cfu/kg)for 28 d.The transcriptomic characteristics of epithelial cells of the cecal mucosa were determined by RNA-sequence,and the cecal microbiota composition was explored by 16 S ribosomal RNA gene sequencing.The changes in the intestinal mucosa of broilers were then analyzed by tissue staining.Gene Ontology(GO)annotations identified substance transport and processes and pathways that might participate in intestinal development and cell viability.Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis revealed that the differentially expressed genes are involved in numerous pathways related to amino acid and vitamin metabolism and antioxidant and defensive functions,among others.The relative expression of some genes associated with intestinal barrier function(claudins 2,15,19,and 23,tight junction proteins 1,2,and 3 and mucin 1)was significantly increased in the treatment group(P<0.05 or P<0.01).Moreover,the proportion of Firmicutes was higher in the C.butyricum-treated group,whereas the proportion of Proteobacteria was higher in the control group.At the genus level,the relative abundances of Butyricicoccus and Lactobacillus,among other bacteria,were increased after C.butyricum supplementation.The tissue staining analysis showed that the cecal mucosa of broilers was significantly ameliorated after the addition of C.butyricum(P<0.05 or P<0.01).These results showed that dietary supplementation with C.butyricum can enhance the antioxidant capacity,mucosal barrier function,and stabilize the cecal microbiota,resulting in improving the growth performance. | Laipeng Xu Xiangli Sun Xianhua Wan Keke Li Fuchun Jian Wenting Li Ruirui Jiang Ruili Han Hong Li Xiangtao Kang Yanbin Wang | 2021 | Animal Nutrition2021，，4： | 12 |
| 2 | Predictive values of multidetector-row computed tomography combined with serum tumor biomarkers in preoperative lymph node metastasis of gastric cancer显示文摘Objective: Multidetector-row computed tomography(MDCT) and serum tumor biomarkers are commonly used to evaluate the preoperative lymph node metastasis and the clinical staging of gastric cancer(GC). This study intends to evaluate the clinical predictive value of MDCT and serum tumor biomarkers in lymph node metastasis of GC.Methods: The clinicopathologic data of 445 GC patients who underwent radical gastrectomy were retrospectively analyzed to evaluate the diagnostic value of MDCT and serum tumor biomarkers in lymph node metastatic staging of GC before surgery.Results: With the multinomial logistic regression analysis, the independent relative factors of lymph node metastasis of GC were identified as tumor size, depth of tumor invasion, vessel invasion, vascular embolus, and soft tissue invasion. The optimal critical value of the short diameter of lymph nodes detected by MDCT scanning for evaluation of preoperative lymph node metastasis was 6.0 mm, with 75.7% as predictive accuracy of lymph node metastasis compared to the postoperative pathological results of GC patients. In addition, the critical value of the short diameter of lymph nodes combined with serum tumor biomarkers [including carbohydrate antigen(CA)-724 and CA-199] could show an enhancement of predictive sensitivity of lymph node metastasis(up to 89.3%) before surgery.Conclusions: MDCT combined with serum tumor biomarkers should be adopted to improve preoperative sensitivity and accuracy of lymph node metastasis for GC patients. | Huihui Bai Jingyu Deng Nannan Zhang Huifang Liu Wenting He Jinyuan Liu Han Liang | 2019 | Chinese Journal of Cancer Research2019，31，3： | 11 |
| 3 | Distinct element method analysis and field experiment of soil resistance applied on the subsoiler显示文摘Since the design of the subsoiler is a complex work,the interaction between the subsoiler and soil was investigated by using Distinct Element Method(DEM)in this study.Based on the traditional discrete element theory,the 3D model of soil particles and the subsoiler were established after considering the liquid bridge force between soil particles.The operating resistance curves of the subsoiler were achieved after the DEM simulation at a speed of 1 m/s,and three depths of 180 mm,220 mm and 260 mm,respectively.The simulation curves agreed well with the field experimental results based on relative errors of 2.96%,14.95%and 7.15%,respectively,at three depths.All these data proved that it was feasible and favorable to analyze the performance of the subsoiler by using the DEM and it is of important significance for studying and further optimizing the structure of the subsoiler. | Li Bo Liu Fanyi Mu Junying Chen Jun Han Wenting | 2014 | International Journal of Agricultural and Biological Engineering2014，7，1： | 9 |
| 4 | An Epidemic of Hepatitis A--Liaoning Province,2020显示文摘What is already known about this topic?Hepatitis A(HA)is caused by acute hepatitis A virus(HAV)infection and was once very common in China.Following the 2008 introduction of the HA vaccine into the national Expanded Program on Immunization(EPI),the incidence of reported HA in China decreased markedly.However,HA epidemics still occur in Liaoning Province every 3–5 years,although with far fewer cases than in the pre-HA-vaccination era.What is added by this report?Between January 1,2020 and March 18,2020,the number of reported cases of HA in Dalian and Dandong cities of Liaoning Province increased significantly compared with the same period in previous years.All cases were sporadic,and cases were seen in nearly every township.The increase in HA occurred one month after local fresh seafood became available with most cases being among adults.A casecontrol study showed that consuming raw or undercooked seafood,clams,snapping shrimp,and oysters were significantly associated with the increase in HA.What are the implications for public health practices?Strengthening health education for residents to avoid consumption of raw seafood and encouraging HAV vaccination of adults aged 20 to 54 years are important to prevent periodic HAV endemic outbreaks.Further multisectoral cooperation must be emphasized on HA surveillance in areas with a high prevalence of HA. | Jing Sun Yuanqiu Li Xing Fang Yan Wang Yinan Han Yang Liu Jingyuan Cao Wenting Zhou Hui Zheng Wenqing Yao | 2020 | China CDC weekly2020，2，30： | 7 |
| 5 | Live cell single molecule-guided Bayesian localization super resolution microscopy显示文摘 | Xu, Fan Zhang, Mingshu He, Wenting Han, Renmin Xue, Fudong Liu, Zhiyong Zhang, Fa Lippincott-Schwartz, Jennifer Xu, Pingyong | 2017 | Cell Research2017，27，5： | 6 |
| 6 | Determination of the draft force for different subsoiler points using discrete element method显示文摘Generally,a subsoiler is comprised of a shank and a point.The point shape has a significant effect on the draft force of a subsoiler.In this study,the draft force of subsoilers with four different points were compared under the speed of 0.8 m/s and the depth of 350 mm in the soil bin.Discrete Element Method(DEM)was applied in simulating the working process of the subsoiler.The stiffness of soil particles used in DEM was calibrated by comparing the simulated draft force of a standard arc-shaped subsoiler with the experiment.The calibrated soil particle stiffness was 1.1×104 N/m.The validated model was then used to compare the draft force of subsoilers with four different points under the same condition in the test.Results showed that different points would cause different draft forces.The subsoiler with short chisel point caused the smallest draft force(2885 N)while the point with short face and wings had the largest force(4474 N).The relative errors of the simulated results were less than 4%,which proved that DEM was an effective way for predicting the draft force of subsilers.The velocity field and contact force filed could show the movement of soil around the subsoiler. | Li Bo Xia Rui Liu Fanyi Chen Jun Han Wenting Han Bing | 2016 | International Journal of Agricultural and Biological Engineering2016，9，3： | 5 |
| 7 | Detecting maize leaf water status by using digital RGB images显示文摘To explore the correlation between crop leaf digital RGB(Red,Green and Blue)image features and the corresponding moisture content of the leaf,a Canon digital camera was used to collect image information from detached leaves of heading-stage maize.A drying method was adopted to measure the moisture content of the leaf samples,and image processing technologies,including gray level co-occurrence matrices and grayscale histograms,was used to extract the maize leaf texture feature parameters and color feature parameters.The correlations of these feature parameters with moisture content were analyzed.It is found that the texture parameters of maize leaf RGB images,including contrast,correlation,entropy and energy,were not significantly correlated with moisture content.Thus,it was difficult to use these features to predict moisture content.Of the six groups of eigenvalues for the leaf color feature parameters,including mean,variance,energy,entropy,kurtosis and skewness,mean and kurtosis were found to be correlated with moisture content.Thus,these features could be used to predict the leaf moisture content.The correlation coefficient(R2)of the mean-moisture content relationship model was 0.7017,and the error of the moisture content prediction was within±2%.The R2 of the kurtosis-moisture content relationship model was 0.7175,and the error of the moisture content prediction was within±1.5%.The study results proved that RGB images of crop leaves could be used to measure moisture content. | Han Wenting Sun Yu Xu Tengfei Chen Xiangwei Su Ki Ooi | 2014 | International Journal of Agricultural and Biological Engineering2014，7，1： | 5 |
| 8 | Quality of terrestrial data derived from UAV photogrammetry:A case study of Hetao irrigation district in northern China显示文摘Most crops in northern China are irrigated,but the topography affects the water use,soil erosion,runoff and yields.Technologies for collecting high-resolution topographic data are essential for adequately assessing these effects.Ground surveys and techniques of light detection and ranging have good accuracy,but data acquisition can be time-consuming and expensive for large catchments.Recent rapid technological development has provided new,flexible,high-resolution methods for collecting topographic data,such as photogrammetry using unmanned aerial vehicles(UAVs).The accuracy of UAV photogrammetry for generating high-resolution Digital Elevation Model(DEM)and for determining the width of irrigation channels,however,has not been assessed.A fixed-wing UAV was used for collecting high-resolution(0.15 m)topographic data for the Hetao irrigation district,the third largest irrigation district in China.112 ground checkpoints(GCPs)were surveyed by using a real-time kinematic global positioning system to evaluate the accuracy of the DEMs and channel widths.A comparison of manually measured channel widths with the widths derived from the DEMs indicated that the DEM-derived widths had vertical and horizontal root mean square errors of 13.0 and 7.9 cm,respectively.UAV photogrammetric data can thus be used for land surveying,digital mapping,calculating channel capacity,monitoring crops,and predicting yields,with the advantages of economy,speed and ease. | Hongming Zhang Jiangtao Yang Jantiene E.M.Baartman Shuqin Li Bei Jin Wenting Han Xiaomei Yang Lingtong Gai Coen J.Ritsema Violette Geissen | 2018 | International Journal of Agricultural and Biological Engineering2018，11，3： | 4 |
| 9 | Model of soybean NDVI change based on time series显示文摘Normalized Difference Vegetation Index(NDVI)has been found to have good correlations with many physical properties of soybean surfaces.Due to the factors of air temperature,humidity,solar radiation,soil moisture,etc.,NDVI of soybean varies dynamically in a day.The establishment of the soybean NDVI prediction model at different times in a day can effectively modify this variation.The soybean NDVI values are continuously monitored in hours during soybean seeding,flowering&podding and maturating stages by way of Green Seeker.Results show that the trend of NDVI change every day in the three stages is taken on as a reverse parabola.The NDVI value reaches to the maximum at 8 am or 9 am and decreases to its minimum at 2 pm before a moderate rise.A model for intraday and long-term NDVI change for soybean is built.The test of the model with independent data indicates that the precision meets the demands,with the root mean square error(RMSE)of each day being 3.95,5.45 and 2.86 for the seeding stage,the bean podding stage and the maturation period,respectively.The prediction RMSEs of the soybean NDVI model for soybeans of the three stages for the fifth day are 5.75,2.65 and 5.51,respectively and the prediction RMSEs for the sixth day are 9.74,2.82 and 14.04,respectively according to the data from the first four days. | Zhang Zhitao Yubin Lan Wu Pute Han Wenting | 2014 | International Journal of Agricultural and Biological Engineering2014，7，5： | 3 |
| 10 | Simple and efficient delivery of cellimpermeable organic fluorescent probes into live cells for live-cell superresolution imaging显示文摘Numerous commercial organic fluorophores with excellent optical properties are precluded from live-cell superresolution imaging due to poor cell permeability.Here,we develop a simple but effective strategy that renders cells permeable to cell-impermeable,organic fluorescent probes by using a novel peptide vehicle,PV-1.By simple coincubation with PV-1,22 different cell-impermeable,organic fluorescent probes were efficiently delivered into live cells and specifically labeled a variety of organelles.Moreover,PV-1 can simultaneously transfer up to three different probes into live cells.By using PV-1 and these cell-impermeable fluorescent probes,we obtained multicolor,longterm,live-cell superresolution images of various organelles,which allowed us to study the dynamic interactions between them.PV-1,together with these organic fluorescent probes,will greatly broaden the applications of superresolution imaging technology in diverse live-cell studies and opens up a new avenue in the design and application of peptide vehicles. | Meng Zhang Meihua Li Wenting Zhang Yubing Han Yu-Hui Zhang | 2019 | Light(Science & Applications)2019，8，1： | 2 |
| 11 | Real-time remote monitoring system for crop water requirement information显示文摘Rapidly acquiring and real-time transmitting crop water requirement information constitute the basis for achieving intelligent diagnosis and precision irrigation.In order to collect and transmit crop water requirement information at real time,a new microcontroller-based real-time remote monitoring system was designed,including system hardware design,software and anti-jamming design.The system achieved the functions including clock reading,information configuration,LCD display,keyboard control,data sending and receiving,multi-channel information acquisition,conversion and storage.Laboratory and field tests showed that the system can achieve data acquisition and real-time display of the crop water requirement information.Unlike the current weather station,the system collects crop water information,meteorological factors and soil parameters at the same time.It has a high level of stability and acquisition accuracy,and can meet the requirements for real-time remote monitoring of the crop water requirement information for irrigation decision-making. | Han Wenting Xu Zhiqing Zhang Yang Cao Pei Chen Xiangwei Su Ki Ooi | 2014 | International Journal of Agricultural and Biological Engineering2014，7，6： | 2 |
| 12 | Estimation of transpiration coefficient and aboveground biomass in maize using time-series UAV multispectral imagery显示文摘Estimating spatial variation in crop transpiration coefficients(CTc) and aboveground biomass(AGB)rapidly and accurately by remote sensing can facilitate precision irrigation management in semiarid regions. This study developed and assessed a novel machine learning(ML) method for estimating CTc and AGB using time-series unmanned aerial vehicle(UAV)-based multispectral vegetation indices(VIs)of maize under several irrigation treatments at the field scale. Four ML regression methods: multiple linear regression(MLR), support vector regression(SVR), random forest regression(RFR), and adaptive boosting regression(ABR), were used to address the complex relationship between CTcand VIs. AGB was then estimated using exponential, logistic, sigmoid, and linear equations because of their clear mathematical formulations based on the optimal CTcestimation model. The UAV VIs-derived CTcusing the RFR estimation model yielded the highest accuracy(R^(2)= 0.91, RMSE = 0.0526, and n RMSE = 9.07%). The normalized difference red-edge index, transformed chlorophyll absorption in reflectance index, and simple ratio contributed significantly to the RFR-based CTcmodel. The accuracy of AGB estimation using nonlinear methods was higher than that using the linear method. The exponential method yielded the highest accuracy(R^(2)= 0.76, RMSE = 282.8 g m, and n RMSE = 39.24%) in both the 2018 and 2019 growing seasons. The study confirms that AGB estimation models based on cumulative CTcperformed well under several irrigation treatments using high-resolution time-series UAV multispectral VIs and can support irrigation management with high spatial precision at a field scale. | Guomin Shao Wenting Han Huihui Zhang Yi Wang Liyuan Zhang Yaxiao Niu Yu Zhang Pei Cao | 2022 | The Crop Journal2022，10，5： | 2 |
| 13 | Efficient production of pronuclear embryos in breeding and nonbreeding season for generating transgenic sheep overexpressing TLR4显示文摘Background: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons(breeding and non-breeding season) on superovulation and the imported exogenous gene on growth.Results: In total of 43 donor ewes and 166 recipient ewes in breeding season, 37 donor ewes and 144 recipient ewes in non-breeding season were selected for super-ovulation and injected embryo transfer to generate transgenic sheep.Our results indicated the no. of embryos recovered of donors and the rate of pronuclear embryos did not show any significant difference between breeding and non-breeding seasons(P > 0.05). The positive rate of exogenous TLR4 tested were 21.21 % and 22.58 % in breeding and non-breeding season by Southern blot. The expression level of TLR4 in the transgenic sheep was 1.5 times higher than in the non-transgenic group(P < 0.05). The lambs overexpressing TLR4 had similar growth performance with non-transgenic lambs, and the blood physiological parameters of transgenic and non-transgenic were both in the normal range and did not show any difference.Conclusions: Here we establish an efficient platform for the production of transgenic sheep by the microinjection of pronuclear embryos during the whole year. The over-expression of TLR4 had no adverse effect on the growth of the sheep. | Yan Li Di Lian Shoulong Deng Xiaosheng Zhang Jinlong Zhang Wenting Li Hai Bai Zhixian Wang Hongping Wu Juncai Fu Hongbing Han Jianzhong Feng Guoshi Liu Ling Lian Zhengxing Lian | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 14 | A dynamic model for sprinkler irrigation water distribution 显示文摘 | Chen Xiangwei Zhang Yang Han Wenting | 2013 | International Agricultural Engineering Journal2013，22，3： | 1 |
| 15 | A dynamic model for sprinkler irrigation water distribution显示文摘 | Chen Xiangwei Zhang Yang Han Wenting | 2013 | International Agricultural Engineering Journal2013，22，3： | 1 |
| 16 | Variable-rate con- trolled sprinklers for precision irrigation 显示文摘 | Han Wenting Fen Hao Wu Pute | 2007 | ASABE Annual In- ternational Meeting Technical Papers2007，，6： | 1 |
| 17 | A survey on wireless sensor network infrastructure for agriculture 显示文摘 | Yu Xiaoqing Wu Pute Han Wenting | 2013 | Computer Standards & Interfaces2013，35，1： | 1 |
| 18 | Appli- cation of dispersive liquid-liquid microextraction for the analysis of organophosphorus pesticides in watermelon and cucumber 显示文摘 | Zhao Ercheng Zhao Wenting Han Lijun | 2007 | Journal of Chromatography A2007，1175，1： | 1 |
| 19 | The mass of our Milky Way显示文摘We perform an extensive review of the numerous studies and methods used to determine the total mass of the Milky Way.We group the various studies into seven broad classes according to their modeling approaches.The classes include:i)estimating Galactic escape velocity using high velocity objects;ii)measuring the rotation curve through terminal and circular velocities;iii)modeling halo stars,globular clusters and satellite galaxies with the spherical Jeans equation and iv)with phase-space distribution functions;v)simulating and modeling the dynamics of stellar streams and their progenitors;vi)modeling the motion of the Milky Way,M31 and other distant satellites under the framework of Local Group timing argument;and vii)measurements made by linking the brightest Galactic satellites to their counterparts in simulations.For each class of methods,we introduce their theoretical and observational background,the method itself,the sample of available tracer objects,model assumptions,uncertainties,limits and the corresponding measurements that have been achieved in the past.Both the measured total masses within the radial range probed by tracer objects and the extrapolated virial masses are discussed and quoted.We also discuss the role of modern numerical simulations in terms of helping to validate model assumptions,understanding systematic uncertainties and calibrating the measurements.While measurements in the last two decades show a factor of two scatters,recent measurements using Gaia DR2 data are approaching a higher precision.We end with a detailed discussion of future developments in the field,especially as the size and quality of the observational data will increase tremendously with current and future surveys.In such cases,the systematic uncertainties will be dominant and thus will necessitate a much more rigorous testing and characterization of the various mass determination methods. | WenTing Wang JiaXin Han Marius Cautun ZhaoZhou Li Miho N.Ishigaki | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，10： | 1 |
| 20 | Prediction of multivariate chaotic time seriesvia radial basis function neural network 显示文摘 | Chen Diyi Han Wenting | 2013 | Mathematics2013，18，4： | 1 |
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