|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Anatomical and chemical characteristics associated with lodging resistance in wheat显示文摘Anatomical and chemical characteristics of stems affect lodging in wheat(Triticum aestivum L.) cultivars. Traits associated with lodging resistance, such as plant height, stem strength, culm wall thickness, pith diameter, and stem diameter, were extensively investigated in earlier studies. However, the solid stem trait was rarely considered. In this study, we measured a range of anatomical and chemical characteristics on solid and hollow stemmed wheat cultivars. Significant correlations were detected between resistance to lodging and several anatomical features, including width of mechanical tissue, weight of low internodes, and width of stem walls. Morphological features that gave the best indication of improved lodging resistance were increased stem width, width of mechanical tissue layer, and stem density. Multiple linear regression analysis showed that 99% of the variation in lodging resistance could be explained by the width of the mechanical tissue layer, suggesting that solid stemmed wheat has several anatomical features for increasing resistance to lodging. In addition, microsatellite markers GWM247 and GWM340 were linked to a single solid stem QTL on chromosome 3BL in a population derived from the cross Xinongshixin(solid stem)/Line 3159(hollow stem). These markers should be valuable in breeding wheat for solid stem. | Eryan Kong Dongcheng Liu Xiaoli Guo Wenlong Yang Jiazhu Sun Xin Li Kehui Zhan Dangqun Cui Jinxing Lin Aimin Zhang | 2013 | The Crop Journal2013，1，1： | 43 |
| 2 | Optimal power allocation for secure directional modulation networks with a full-duplex UAV user显示文摘This paper makes an investigation of a secure unmanned aerial vehicle(UAV)-aided communication network based on directional modulation(DM). In this network, ground base station(GBS) acts as a control center to transmit confidential message and artificial noise(AN). The UAV user, moving along a linear flight trajectory, is intended to receive the useful information from GBS. At the same time, it also sends AN signals to further interference eavesdropper’s channel. Aiming at maximizing secrecy rate during the UAV flight process, a joint optimization problem is formulated with respect to power allocation(PA)factors, beamforming vector and AN projection matrices. For simplicity, maximum rate transmission, nullspace projection and the leakage-based method are applied to form the transmit beamforming vector, AN projection matrix at GBS, and AN projection vector at UAV user, respectively. Following this, the optimization problem reduces to a bivariate optimization programme with two PA factors. An alternating iterative algorithm(AIA) is proposed to optimize the two PA factors. Simulation results demonstrate that, compared to the half-duplex(HD) mode, the proposed strategy for full-duplex(FD) mode achieves a higher secrecy rate(SR) and outperforms the FD mode with fixed PA strategy. | Zaoyu LU Linlin SUN Shuo ZHANG Xiaobo ZHOU Jinyong LIN Wenlong CAI Jin WANG Jinhui LU Feng SHU | 2019 | Science China(Information Sciences)2019，62，8： | 6 |
| 3 | Experimental and numerical studies on progressive debonding of grouted rock bolts显示文摘Understanding the mechanism of progressive debonding of bolts is of great significance for underground safety.In this paper,both laboratory experiment and numerical simulation of the pull-out tests were performed.The experimental pull-out test specimens were prepared using cement mortar material,and a relationship between the pull-out strength of the bolt and the uniaxial compressive strength(UCS)of cement mortar material specimen was established.The locations of crack developed in the pull-out process were identified using the acoustic emission(AE)technique.The pull-out test was reproduced using 2D Particle Flow Code(PFC^(2D))with calibrated parameters.The experimental results show that the axial displacement of the cement mortar material at the peak load during the test was approximately 5 mm for cement-based grout of all strength.In contrast,the peak load of the bolt increased with the UCS of the confining medium.Under peak load,cracks propagated to less than one half of the anchorage length,indicating a lag between crack propagation and axial bolt load transmission.The simulation results show that the dilatation between the bolt and the rock induced cracks and extended the force field along the anchorage direction;and,it was identified as the major contributing factor for the pull-out failure of rock bolt. | Hao Shi Lei Song Houquan Zhang Wenlong Chen Huasheng Lin Danqi Li Guozhu Wang Huayun Zhao | 2022 | International Journal of Mining Science and Technology2022，32，1： | 4 |
| 4 | Clinical data analysis reveals the role of OGR1 (GPR68) in head and neck squamous cancer显示文摘Background:Head and neck squamous cancer(HNSC)frequently occurs in the clinic.Revealing the role of the genes that correlate with cancer cell outgrowth will contribute to potential treatment target identification and tumor inhibition.Methods:The gene expression profiles and gene ontology of the proton-sensing G-protein-coupled receptor OGR1 were analyzed using the TCGA(The Cancer Genome Atlas)database.The effects of sex,age,race,and degree of malignancy on HNSC were investigated,and the survival times of HNSC patients with high or low/medium expression levels of OGR1 were compared.Methylation of the OGR1 promoter CpG sites was also investigated and OGR1-related genes were analyzed using gene set enrichment analysis.Results:OGR1 is overexpressed in HNSC patients.However,compared with the low/median expression group,the high OGR1 expression group did not have different survival rates.The OGR1 expression level differed across sex,age,race,and degree of malignancy,while the methylation of the OGR1 promoter CpG sites was maintained at a similar level.Gene set enrichment analysis revealed that OGR1 was positively correlated with head and neck cancer,cisplatin resistance,hypoxia,angiogenesis,cell migration,and TGF-β.Conclusion:The expression of OGR1 correlated with HNSC progression and survival and thus can serve as a potential treatment target and prognostic marker. | Wenlong Zhang Yong Han Weisha Li Lin Cao Libo Yan Chuan Qin Ran Gao | 2020 | Animal Models and Experimental Medicine2020，3，1： | 2 |
| 5 | Downregulation of HNRNPK in human cancer cells inhibits lung metastasis显示文摘Background: Lung cancer frequently occurs in the clinic, leading to poor prognosis and high mortality. Markers for early diagnosis of lung cancer are scarce, and further potential therapeutic targets are also urgently needed.Method: We established a new mouse model in which the specific gene HNRNPK(heterogeneous nuclear ribonucleoprotein K) was downregulated after administration of doxycycline. The lung metastatic nodules were investigated using bioluminescence imaging, micro-CT, and autopsy quantification.Results: Compared with the short hairpin negative control group, less lung metastatic nodules were formed in the short hairpin RNA group.Conclusion: Downregulation of HNRNPK in cancer cells can inhibit lung metastasis. | Mengyuan Li Wenlong Zhang Xingjiu Yang Hongfei Liu Lin Cao Weisha Li Le Wang Guoxin Zhang Ran Gao | 2019 | Animal Models and Experimental Medicine2019，2，4： | 2 |
| 6 | FXYD3 enhances IL-17A signaling to promote psoriasis by competitively binding TRAF3 in keratinocytes显示文摘Psoriasis is a common chronic inflammatory skin disease characterized by inflammatory cell infiltration and epidermal hyperplasia.However,the regulatory complexity of cytokine and cellular networks still needs to be investigated.Here,we show that the expression of FXYD3,a member of the FXYD domain-containing regulators of Na+/K+ATPases family,is significantly increased in the lesional skin of psoriasis patients and mice with imiquimod(IMQ)-induced psoriasis.IL-17A,a cytokine important for the development of psoriatic lesions,contributes to FXYD3 expression in human primary keratinocytes.FXYD3 deletion in keratinocytes attenuated the psoriasis-like phenotype and inflammation in an IMQ-induced psoriasis model.Importantly,FXYD3 promotes the formation of the IL-17R-ACT1 complex by competing with IL-17R for binding to TRAF3 and then enhances IL-17A signaling in keratinocytes.This promotes the activation of the NF-κB and MAPK signaling pathways and leads to the expression of proinflammatory factors.Our results clarify the mechanism by which FXYD3 serves as a mediator of IL-17A signaling in keratinocytes to form a positive regulatory loop to promote psoriasis exacerbation.Targeting FXYD3 may serve as a potential therapeutic approach in the treatment of psoriasis. | Wenjuan Yang Rukun He Hao Qu Wenwen Lian Yue Xue Tao Wang Wenlong Lin Peishuo Zhu Meng Xia Lihua Lai Qingqing Wang | 2023 | Cellular & Molecular Immunology2023，20，3： | 2 |
| 7 | Pelagic-benthic coupling of the microbial food web modifies nutrient cycles along a cascade-dammed river显示文摘Cascade dams disrupt the river continuum,altering hydrology,biodiversity and nutrient flux.Describing the diversity of multi-trophic microbiota and assessing microbial contributions to the ecosystem processes are prerequisites for the restoration of these aquatic systems.This study investigated the microbial food web structure along a cascade-dammed river,paying special attention to the multi-trophic relationships and the potential role of pelagic-benthic coupling in nutrient cycles.Our results revealed the discontinuity in bacterial and eukaryotic community composition,functional group proportion,as well as a-diversity due to fragmentation by damming.The high microbial dissimilarity along the river,with the total multi-trophic P-diversity was 0.84,was almost completely caused by species replacement.Synchronization among trophic levels suggests potential interactions of the pelagic and the benthic groups,of which the p-diversities were primarily influenced by geographic and environmental factors,respectively.Dam-induced environmental variations,especially hydrological and nutrient variables,potentially influence the microbial food web via both top-down and bottom-up forces.We proposed that the cycles of carbon,nitrogen and phosphorus are influenced by multi-trophic groups through autotrophic and heterotrophic processes,predator-prey relationships,as well as the release of nutrients mainly by microfauna.Our results advance the notion that pelagic-benthic trophic coupling may intensify the accumulation of organic carbon,ammonium and inorganic phosphorus,thereby changing the biogeochemical patterns along river systems.As a consequence,researchers should pay more attention to the multi-trophic studies when assessing the environmental impacts,and to provide the necessary guidance for the ecological conservation and restoration of the dam-regulated systems. | Nan Yang Linqiong Wang Li Lin Yi Li Wenlong Zhang Lihua Niu Huanjun Zhang Longfei Wang | 2022 | Frontiers of Environmental Science & Engineering2022，16，4： | 1 |
| 8 | Effects of microencapsulated APP-II on the microstructure and flame retardancy of PP/APP–II/PER composites显示文摘 | Yang Lin Cheng Wenlong Zhou Jian | 2014 | Polymer Degradation and Stability2014，105，： | 1 |
| 9 | Programmable robotized‘transfer-and-jet’printing for large,3D curved electronics on complex surfaces显示文摘Large,3D curved electronics are a trend of the microelectronic industry due to their unique ability to conformally coexist with complex surfaces while retaining the electronic functions of 2D planar integrated circuit technologies.However,these curved electronics present great challenges to the fabrication processes.Here,we propose a reconfigurable,mask-free,conformal fabrication strategy with a robot-like system,called robotized‘transfer-and-jet’printing,to assemble diverse electronic devices on complex surfaces.This novel method is a ground-breaking advance with the unique capability to integrate rigid chips,flexible electronics,and conformal circuits on complex surfaces.Critically,each process,including transfer printing,inkjet printing,and plasma treating,are mask-free,digitalized,and programmable.The robotization techniques,including measurement,surface reconstruction and localization,and path programming,break through the fundamental constraints of 2D planar microfabrication in the context of geometric shape and size.The transfer printing begins with the laser lift-off of rigid chips or flexible electronics from donor substrates,which are then transferred onto a curved surface via a dexterous robotic palm.Then the robotic electrohydrodynamic printing directly writes submicrometer structures on the curved surface.Their permutation and combination allow versatile conformal microfabrication.Finally,robotized hybrid printing is utilized to successfully fabricate a conformal heater and antenna on a spherical surface and a flexible smart sensing skin on a winged model,where the curved circuit,flexible capacitive and piezoelectric sensor arrays,and rigid digital–analog conversion chips are assembled.Robotized hybrid printing is an innovative printing technology,enabling additive,noncontact and digital microfabrication for 3D curved electronics. | YongAn Huang Hao Wu Chen Zhu Wennan Xiong Furong Chen Lin Xiao Jianpeng Liu Kaixin Wang Huayang Li Dong Ye Yongqing Duan Jiankui Chen Hua Yang Wenlong Li Kun Bai Zhouping Yin Han Ding | 2021 | International Journal of Extreme Manufacturing2021，3，4： | 1 |
| 10 | Base-free oxidative esterification of 5-hydroxymethylfurfural to furan-2,5-dimethylcarboxylate over n-doped carbon-supported Co/Fe bimetallic catalyst under batch-operation or continuous-flow conditions显示文摘Developing an efficient and easily available catalyst for the selective conversion of biomass-derived 5-hydroxymethylfurfural(HMF)into furan-2,5-dimethylcarboxylate(FDMC),a valuable biomass-based monomer,remains a high demand but formidable challenge.Herein,a facile strategy for the synthesis of N-doped carbon-supported Co/Fe bimetallic catalyst(CoFe-NC)was developed,which provided an outstanding FDMC yield of 93%in a batch reactor(base-free,80℃,2 bar O_(2),4 h).Interestingly,CoFe-NC also gave a high FDMC yield of 91%under continuous-flow conditions for 80 h(5 bar O_(2),80℃,GHSV 1320 h^(-1),LHSV 0.6 h^(-1),base-free).Notably,it is the first time that a non-noble catalyst gave such a high FDMC yield under continuous-flow conditions.The introduction of Fe could greatly improve both the electron intensity of Co-N_(x)species and basicity of the catalyst,which endowed CoFe-NC with improved O_(2)activation capacity and enhanced dehydrogenation activity for the oxidation-esterification of HMF.This work delineates the efficient strategy on the construction of N-doped carbon-supported non-noble catalyst,which might open a new avenue for developing highly efficient catalyst for FDMC production. | Weizhen Xie Binglin Chen Wenlong Jia Huai Liu Zheng Li Shuliang Yang Xing Tang Xianhai Zeng Yong Sun Xixian Ke Tianyuan Li Huayu Fang Lu Lin | 2022 | Journal of Energy Chemistry2022，，12： | 1 |
| 11 | Homogeneous generalized yield criterion based elastic modulus reduction method for limit analysis of thin-walled structures with angle steel显示文摘 | LuFeng Yang Qi Li Wei Zhang Wenlong Wu Yinhe Lin | 2014 | Thin-Walled Structures2014，，： | 1 |
| 12 | An Artificial Reflex Arc That Perceives Afferent Visual and Tactile Information and Controls Efferent Muscular Actions显示文摘Neural perception and action-inspired electronics is becoming important for interactive human-machine interfaces and intelligent robots.A system that implements neuromorphic environmental information coding,synaptic signal processing,and motion control is desired.Wereport aneuroinspired artifcialreflex arcthat possesses visual and'somatosensory dual aferent nerve paths and an eferent nerve path to control artificial muscles.A self-powered photoelectric synapse between the afferent and efferent nerves was used as thekey information processor.The artificial reflex arc successfully responds to external visual and tactile information and controls the actions of artificial muscle in response to these external stimuli and thus emulates reflex activities through a full refex arc.The visual and somatosensory information is encoded as impulse spikes,the frequency of which exhibited a sublinear dependence on the obstacle proximity orpressure stimuli.The artificial reflex arc suggests a promising strategy toward developing soft neurorobotic systems and prostheses. | Lin Sun Yi Du Huanhuan Wei Wenlong Xu Wentao Xu | 2022 | Research2022，，2： | 1 |
| 13 | Optics‑guided Robotic System for Dental Implant Surgery显示文摘At present,dental implant surgery mainly relies on the clinical experience of the doctor and the assistance of preoperative medical imaging.However,there are some problems in dental implant surgery,such as narrow space,sight obstruction,inaccurate positioning,and high requirements of doctors’proficiency.Therefore,a dental implant robot system(DIRS)guided by optical navigation is developed in this study,with an x-shaped tool and an irregular pentagonal tracer are designed for spatial registration and needle tip positioning strategy respectively.The coordinate system of each unit in DIRS is unified through system calibration,spatial registration,and needle tip positioning strategy.Then the surgical path is planned on the computed tomography(CT)images in the navigation software before operation.The automatic positioning method and the auxiliary positioning method can be used in the operation to achieve accurate positioning and assist doctors to complete the operation.The errors of spatial registration,needle tip positioning strategy,and the overall accuracy of the system were evaluated respectively,and the results showed that they all met the needs of clinical surgery.This study preliminarily verified the feasibility of the precise positioning method for dental surgery robots and provided certain ideas for subsequent related research. | Biao Yan Wenlong Zhang Lijing Cai Lingxiang Zheng Kaiyang Bao Yuming Rao Lin Yang Weitao Ye Peifeng Guan Wei Yang Jiang Li Rongqian Yang | 2022 | Chinese Journal of Mechanical Engineering2022，35，3： | 1 |
| 14 | Crosstalk between IL-15Rα^(+)tumor-associated macrophages and breast cancer cells reduces CD8+T cell recruitment显示文摘Background:Interleukin-15(IL-15)is a promising immunotherapeutic agent owing to its powerful immune-activating effects.However,the clinical benefits of these treatments are limited.Crosstalk between tumor cells and immune cells plays an important role in immune escape and immunotherapy drug resistance.Herein,this study aimed to obtain in-depth understanding of crosstalk in the tumor microenvironment for providing potential therapeutic strategies to prevent tumor progression.Methods:T-cell killing assays and co-culture models were developed to determine the role of crosstalk between macrophages and tumor cells in breast cancer resistant to IL-15.Western blotting,histological analysis,CRISPR-Cas9 knockout,multi-parameter flow cytometry,and tumor cell-macrophage co-injection mouse models were developed to examine the mechanism by which IL-15Rα^(+)tumor-associated macrophages(TAMs)regulate breast cancer cell resistance to IL-15.Results:We found thatmacrophages contributed to the resistance of tumor cells to IL-15,and tumor cells induced macrophages to express high levels of theαsubunit of the IL-15 receptor(IL-15Rα).Further investigation showed that IL-15Rα^(+)TAMs reduced the protein levels of chemokine CX3C chemokine ligand 1(CX3CL1)in tumor cells to inhibit the recruitment of CD8^(+)T cells by releasing the IL-15/IL-15Rαcomplex(IL-15Rc).Administration of an IL-15Rc blocking peptide markedly suppressed breast tumor growth and overcame the resistance of cancer cells to anti-programmed cell death protein 1(PD-1)antibody immunotherapy.Interestingly,Granulocyte-macrophage colony-stimulating factor(GMCSF)inducedγchain(γc)expression to promote tumor cell-macrophage crosstalk,which facilitated tumor resistance to IL-15.Additionally,we observed that the non-transcriptional regulatory function of hypoxia inducible factor-1alpha(HIF-1α)was essential for IL-15Rc to regulateCX3CL1 expression in tumor cells.Conclusions:The IL-15Rc-HIF-1α-CX3CL1 signaling pathway serves as a crosstalk between macrophages and tumor cells in the tumormicroenvironment of breast cancer.Targeting this pathway may provide a potential therapeutic strategy for enhancing the efficacy of cancer immunotherapy. | Wenlong Zhang Qing Zhang Nanfei Yang Qian Shi Huifang Su Tingsheng Lin Zhonglei He Wenxin Wang Hongqian Guo Pingping Shen | 2022 | Cancer Communications2022，42，6： | 0 |
| 15 | Study on Superheavy Nuclide 271Ds显示文摘 | Zhang Zhiyuan Gan Zaiguo Ma Long Huang Minghui Huang Tianheng Wu Xiaolei Jia Guobin Li Guangshun Yu Lin Ren Zhongzhou Zhou Shangui Zhang Yuhu Zhou Xiaohong Xu Hushan Zhang Huanqiao Xiao Guoqing Zhan Wenlong | 2011 | IMP & HIRFL Annual Report2011，，1： | 0 |
| 16 | Genome resequencing reveals the evolutionary history of garlic reproduction traits显示文摘The propagation of cultivated garlic relies on vegetative cloves,thus flowers become non-essential for reproduction in this species,driving the evolution of reproductive feature-derived traits.To obtain insights into the evolutionary alteration of reproductive traits in the clonally propagated garlic,the evolutionary histories of two main reproduction-related traits,bolting and flower differentiation,were explored by genome analyses using 134 accessions displaying wide diversity in these two traits.Resequencing identified 272.8 million variations in the garlic genome,198.0million of which represent novel variants.Population analysis identified five garlic groups that have evolved into two clades.Gene expression,single-cell transcriptome sequencing,and genome-wide trait association analyses have identified numerous candidates that correlate with reproductive transition and flower development,some of which display distinct selection signatures.Selective forces acting on the B-box zinc finger protein-encoding Asa2G00291.1,the global transcription factor group E protein-encoding Asa5G01527.1,and VERNALIZATION INSENSITIVE 3-like Asa3G03399.1 appear to be representative of the evolution of garlic bolting.Plenty of novel genomic variations and trait-related candidates represent valuable resources for biological studies of garlic.Numerous selective signatures from genes associated with the two chosen reproductive traits provide important insights into the evolutionary history of reproduction in this clonally propagated crop. | Haiping Wang Einat Shemesh-Mayer Jiangjiang Zhang Song Gao Zheng Zeng Zemao Yang Xueyu Zhang Huixia Jia Yanzhou Wang Jiangping Song Xiaohui Zhang Wenlong Yang Qiaoyun He Amir Sherman Lin Li Rina Kamenetsky Touming Liu | 2023 | Horticulture Research2023，10，11： | 0 |
| 17 | A photonanozyme with light-empowered specific peroxidasemimicking activity显示文摘Although nanozymes have been widely developed,directly utilizing light to drive catalytic reactions like natural photoenzymes still remains challenging.Herein,we propose that photonanozymes(PNZs),as a novel kind of nanozyme,exclusively possess enzyme-mimicking activity under illumination.Only in the presence of visible light,the as-synthesized TiO_(2) proposed in this contribution shows excellent specificity of peroxidase-like without any oxidase-or catalase-like activity.The driving force of the light-empowered peroxidase-like photonanozymatic activity is explicated in terms of the photogenerated hot charge carriers in TiO_(2) PNZs and the accompanied reactive oxygen species.The co-substrates for photonanozymatic reaction over TiO_(2) PNZs facilitate the formation of the precarious and reactive peroxo-oxygen bridge between TiO_(2) and H_(2)O_(2),enabling the catalytic specificity.With the TiO_(2) PNZ-based biosensing platform for visual glucose detection exemplifying the concept of the application of PNZs,this work may evoke more inspirations to explore strategies for enlarging the scope of photoenzyme mimics. | Sili Lin Wenlong Tan Pengfei Han Xu Li Jinzhao Li Zhou Nie Kun Li | 2022 | Nano Research2022，15，10： | 0 |
| 18 | Nonlinear chirped pulse amplification for a 100-W-class GHz femtosecond all-fiber laser system at 1.5μm显示文摘In this work,we present a high-power,high-repetition-rate,all-fiber femtosecond laser system operating at 1.5μm.This all-fiber laser system can deliver femtosecond pulses at a fundamental repetition rate of 10.6 GHz with an average output power of 106.4 W–the highest average power reported so far from an all-fiber femtosecond laser at 1.5μm,to the best of our knowledge.By utilizing the soliton-effect-based pulse compression effect with optimized pre-chirping dispersion,the amplified pulses are compressed to 239 fs in an all-fiber configuration.Empowered by such a high-power ultrafast fiber laser system,we further explore the nonlinear interaction among transverse modes LP01,LP11 and LP21 that are expected to potentially exist in fiber laser systems using large-mode-area fibers.The intermodal modulational instability is theoretically investigated and subsequently identified in our experiments.Such a high-power all-fiber ultrafast laser without bulky free-space optics is anticipated to be a promising laser source for applications that specifically require compact and robust operation. | Yiheng Fan Hao Xiu Wei Lin Xuewen Chen Xu Hu Wenlong Wang Junpeng Wen Hao Tian Molei Hao Chiyi Wei Luyi Wang Xiaoming Wei Zhongmin Yang | 2023 | High Power Laser Science and Engineering2023，11，4： | 0 |
| 19 | 5mC DNA methylation modification-mediated regulation in tissue functional differentiation and important flavor substance synthesis of tea plant (Camellia sinensis L.)显示文摘In plants,5mC DNA methylation is an important and conserved epistatic mark involving genomic stability,gene transcriptional regulation,developmental regulation,abiotic stress response,metabolite synthesis,etc.However,the roles of 5mC DNA methylation modification(5mC methylation)in tea plant growth and development(in pre-harvest processing)and flavor substance synthesis in pre-and post-harvest processing are unknown.We therefore conducted a comprehensive methylation analysis of four key pre-harvest tissues(root,leaf,flower,and fruit)and two processed leaves during oolong tea post-harvest processing.We found that differential 5mC methylation among four key tissues is closely related to tissue functional differentiation and that genes expressed tissue-specifically,responsible for tissue-specific functions,maintain relatively low 5mC methylation levels relative to non-tissue-specifically expressed genes.Importantly,hypomethylation modifications of CsAlaDC and TS/GS genes in roots provided the molecular basis for the dominant synthesis of theanine in roots.In addition,integration of 5mC DNA methylationomics,metabolomics,and transcriptomics of post-harvest leaves revealed that content changes in flavor metabolites during oolong tea processing were closely associated with transcription level changes in corresponding metabolite synthesis genes,and changes in transcript levels of these important synthesis genes were strictly regulated by 5mC methylation.We further report that some key genes during processing are regulated by 5mC methylation,which can effectively explain the content changes of important aroma metabolites,includingα-farnesene,nerolidol,lipids,and taste substances such as catechins.Our results not only highlight the key roles of 5mC methylation in important flavor substance synthesis in pre-and post-harvest processing,but also provide epimutation-related gene targets for future improvement of tea quality or breeding of whole-tissue high-theanine varieties. | Weilong Kong Qiufang Zhu Qing Zhang Yiwang Zhu Jingjing Yang Kun Chai Wenlong Lei Mengwei Jiang Shengcheng Zhang Jinke Lin Xingtan Zhang | 2023 | Horticulture Research2023，10，8： | 0 |
| 20 | Construction of a high-density genetic linkage map and identification of flowering-related QTL in erect milkvetch(Astragalus adsurgens)显示文摘Erect milkvetch(Astragalus adsurgens)is a perennial legume forage crop with economic and ecological value in livestock grazing and soil-erosion control in arid and semiarid areas worldwide.Genomic information and molecular tools to support breeding and research in the species are limited.The objectives of this investigation were to map its genome using DNA markers and to identify quantitative trait loci(QTL)in the species.An F1 mapping population of 250 plants was developed from a cross between two parents with differing flowering-related traits.A high-density genetic linkage map containing 4821 markers on eight linkage groups(LGs)with a total genetic length of 1395 cM and a mean interval of 0.29 cM between adjacent markers was constructed with SLAF-seq technology.Comparative genomic analyses revealed the highest genome sequence similarity(8.71%)between erect milkvetch and Medicago truncatula,followed by Glycine max(7.65%),Cicer arietinum(7.53%),and Lupinus angustifolius(5.21%).A total of 64 significant QTL for flowering-related traits on six LGs were detected,accounting for 9.38 to 19.1%of the associated phenotype variation.Five and 48 key candidate genes for floret number and inflorescence length were identified based on the Glycyrrhiza uralensis genome.These candidate genes were involved in ubiquitination/degradation,pollen development,cell division,cytokinin biosynthetic process,and plant flowering.These findings shed light on the regulation of flowering traits in erect milkvetch and provide genomic resources for future molecular breeding of the crop. | Wenlong Gong Lin Ma Qiu Gao Bao Wei Jiangui Zhang Xiqiang Liu Pan Gong Zan Wang Guiqin Zhao | 2022 | The Crop Journal2022，10，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员