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| 1 | Baicalin induces ferroptosis in bladder cancer cells by downregulating FTH1显示文摘Ferroptosis is a non-apoptotic regulated cell death caused by iron accumulation and subsequent lipid peroxidation.Currently,the therapeutic role of ferroptosis on cancer is gaining increasing interest.Baicalin an active component in Scutellaria baicalensis Georgi with anticancer potential various cancer types;however,the effects of baicalein on bladder cancer and the underlying molecular mechanisms remain largely unknown.In the study,we investigated the effect of baicalin on bladder cancer cells5637 and KU-19-19.As a result,we show baicalin exerted its anticancer activity by inducing apoptosis and cell death in bladder cancer cells.Subsequently,we for the first time demonstrate baicalin-induced ferroptotic cell death in vitro and in vivo,accompanied by reactive oxygen species(ROS) accumulation and intracellular chelate iron enrichment.The ferroptosis inhibitor deferoxamine but not necrostatin-1,chloroquine(CQ),N-acetyl-L-cysteine,L-glutathione reduced,or carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]-fluoromethylketone(Z-VAD-FMK) rescued baicalin-induced cell death,indicating ferroptosis contributed to baicalin-induced cell death.Mechanistically,we show that ferritin heavy chain1(FTH1) was a key determinant for baicalin-induced ferroptosis.Overexpression of FTH1 abrogated the anticancer effects of baicalin in both 5637 and KU19-19 cells.Taken together,our data for the first time suggest that the natural product baicalin exerts its anticancer activity by inducing FTH1-dependent ferroptosis,which will hopefully provide a prospective compound for bladder cancer treatment. | Na Kong Xiaying Chen Jiao Feng Ting Duan Shuiping Liu Xueni Sun Peng Chen Ting Pan Lili Yan Ting Jin Yu Xiang Quan Gao Chengyong Wen Weirui Ma Wencheng Liu Mingming Zhang Zuyi Yang Wengang Wang Ruonan Zhang Bi Chen Tian Xie Xinbing Sui Wei Tao | 2021 | Acta Pharmaceutica Sinica B2021，11，12： | 23 |
| 2 | Curcumenol triggered ferroptosis in lung cancer cells via lncRNA H19/miR-19b-3p/FTH1 axis显示文摘Curcumenol,an effective ingredient of Wenyujin,has been reported that exerted its antitumor potential in a few cancer types.However,the effect and molecular mechanism of curcumenol in lung cancer are largely unknown.Here,we found that curcumenol induced cell death and suppressed cell proliferation in lung cancer cells.Next,we demonstrated that ferroptosis was the predominant method that contributed to curcumenol-induced cell death of lung cancer in vitro and vivo for the first time.Subsequently,using RNA sequencing,we found that the long non-coding RNA H19(lncRNA H19)was significantly downregulated in lung cancer cells treated with curcumenol,when compared to untreated controls.Overexpression of lncRNA H19 eliminated the anticancer effect of curcumenol,while lncRNA H19 knockdown promoted ferroptosis induced by curcumenol treatment.Mechanistically,we showed that lncRNA H19 functioned as a competing endogenous RNA to bind to miR-19b-3p,thereby enhanced the transcription activity of its endogenous target,ferritin heavy chain 1(FTH1),a marker of ferroptosis.In conclusion,our data show that the natural product curcumenol exerted its antitumor effects on lung cancer by triggering ferroptosis,and the lncRNA H19/miR-19b-3p/FTH1 axis plays an essential role in curcumenol-induced ferroptotic cell death.Therefore,our findings will hopefully provide a valuable drug for treating lung cancer patients. | Ruonan Zhang Ting Pan Yu Xiang Mingming Zhang Han Xie Zimao Liang Bi Chen Cong Xu Jing Wang Xingxing Huang Qianru Zhu Ziming Zhao Quan Gao Chengyong Wen Wencheng Liu Weirui Ma Jiao Feng Xueni Sun Ting Duan Elaine Lai-Han Leung Tian Xie Qibiao Wu Xinbing Sui | 2022 | Bioactive Materials2022，7，7： | 8 |
| 3 | Permeabilization of Escherichia coli with ampicillin for a whole cell biocatalyst with enhanced glutamate decarboxylase activity显示文摘The activity of whole-cell biocatalysts is strongly compromised by the cell envelope, which is a permeability barrier against the diffusion of substrates and products. Although common chemical or physical permeabilization methods used in cultured cells enhance cell permeability, these methods inevitably add several extra processing steps after cell cultivation, as well as impede large scale processing. To increase membrane permeability and cellbound glutamate decarboxylase(GAD) activity of recombinant Escherichia coli(BL21(DE3)-p ET28a-gad B) cells without the need for an additional permeabilization step, we investigated the permeabilizing effects of adding cell wall synthesis inhibitors or surfactants to the culture media. Ampicillin was the most effective at improving cell-bound GAD activity of the BL21(DE3)-p ET28a-gad B, although it decreased the cell biomass yield. The best permeabilization effect was observed using an ampicillin concentration of 5 μg·ml-1. Using this concentration,the cell biomass did decrease by 40.58%, but the cell-bound GAD activity of BL21(DE3)-p ET28a-gad B and total cell-bound GAD activity per milliliter of culture was enhanced by 6.24- and 3.64-fold, respectively. Treatment of BL21(DE3)-p ET28a-gad B cells with 5 μg·ml-1ampicillin resulted in structural changes to the cell envelope,but did not substantially affect GAD expression. By entrapping the ampicillin-treated cells in an open pore gelation matrix, which is a polymer derived from polyvinyl alcohol(PVA), alginate, and boric acid, the transformation rate of γ-aminobutyric acid(GABA) at the 10 th cycle produced by immobilized and permeabilized cells remained 46% of the first cycle. GAD activity of the immobilized, permeabilized cells remained over 90% after30 days of storage at 4 °C. | Weirui Zhao Sheng Hu Jun Huang Piyu Ke Shanjing Yao Yinlin Lei Lehe Mei Jinbo Wang | 2016 | Chinese Journal of Chemical Engineering2016，24，7： | 2 |
| 4 | The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germ protein显示文摘 | JIA Junqiang MA Haile ZHAO Weirui | 2010 | Food Chemistry2010，119，： | 1 |
| 5 | ACE-inhibitory peptides produetion from defatted wheat germ protein by continuous coupling of enzymatic hydrolysis and membrane separation:Modeling and ex- perimental studies显示文摘 | Wenjuan Qu Haile Ma Weirui Zhao | 2013 | Chemieal Engineering Journal2013，226，： | 1 |
| 6 | Partial retrieval of CAD models based on the gradient flows in Lie group显示文摘 | Songqiao Tao Zhengdong Huang Bingquan Zuo Yangping Peng Weirui Kang | 2011 | Pattern Recognition2011，，4： | 1 |
| 7 | Global exponential stability analysis of Cohen–Grossberg neural network with delays显示文摘 | Weirui Zhao | 2006 | Communications in Nonlinear Science and Numerical Simulation2006，，5： | 1 |
| 8 | Organizational evolution of project management teams over the whole lifecycle of megaprojects:Case study of the Hong Kong-Zhuhai-Macao Bridge显示文摘Project management teams are critical in the implementation of megaprojects,but their evolution throughout the project lifecycle has not been clearly explained.This paper explores the organizational evolution of megaproject management teams through a longitudinal retrospective case study of the Hong Kong–Zhuhai–Macao Bridge(HZMB)project.The organizational evolution is examined in terms of management objectives,management content,and organizational structure.The organizational evolution of the HZMB project management team exhibits stage differentiation with the coexistence of turbulence and stability.Changes in the external environment are the driving force for organizational evolution,whereas a flexible organizational strategy is critical in promoting this evolution.Basing on the HZMB case study,this paper summarizes six critical measures that facilitate the organizational evolution of megaproject management teams.Our findings add value to megaproject management theory and provide a better understanding of the dynamics and complexity of megaproject organizational management. | Zeyu WANG Huan LAN Minggong ZHANG Weirui XUE Xiaolong XUE | 2023 | Frontiers of Engineering Management2023，10，3： | 1 |
| 9 | The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germprotein显示文摘 | Jia Junqiang Ma Haile Zhao Weirui | 2010 | Food Chemistry2010，119，： | 1 |
| 10 | The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germ protein显示文摘 | Junqiang Jia Haile Ma Weirui Zhao Zhenbin Wang Wanmin Tian Lin Luo Ronghai He | 2009 | Food Chemistry2009，，1： | 1 |
| 11 | Global exponential stability analysis of Cohen-Grossberg neural networkswith delays 显示文摘 | ZNAO Weirui | 2006 | Communications in Nonlinear Science and Numerical Simulation2006，13，： | 1 |
| 12 | ACE - inhibitory pep- tides production from defatted wheat germ protein by continu- ous coupling of enzymatic hydrolysis and membrane separa- tion : Modeling and experimental studies 显示文摘 | Qu Wenjuan Ma Haile Weirui Zhao | 2013 | Chemical Engi- neering Journal2013，，116： | 1 |
| 13 | An AIE singlet oxygen generation system based on supramolecular strategy显示文摘The design of supramolecular system s with efficient singlet oxygen generation has attracted considerable interests.Herein,an AIE-based singlet oxygen generation system with chemiluminescence properties is reported in aqueous media based on supramolecular host-guest assembly between a water-soluble pillar[5]arene(WP5)and an AIE photosensitizer(TPEDM).The formed supramolecular nanoparticles exhibit significant singlet oxygen generation ability as well as enhanced fluorescence.In addition,by introducing catalase,this H_(2) O_(2)-responsive supramolecular system shows increased~1 O_(2) generation efficiency compared with the blank nanoparticles.An efficient chemiluminescence system can also be achieved by entrapping an energy donor adamantane derivative(AMPPD).Moreover,the present system can function as nanoreactors to perform the photooxidation of dopamine to form polydopamine with visible light irradiation.This wo rk provides a new strategy for the construction of~1 O_(2) generation system based on supramolecular nanomaterials,which has potential applications in the fields such as chemiluminescence imaging and controlled photocatalysis. | Minzan Zuo Weirui Qian Min Hao Kaiya Wang Xiao-Yu Hu Leyong Wang | 2021 | Chinese Chemical Letters2021，32，4： | 1 |
| 14 | The use of ultrasound for enzymatic preparation of ACE - inhibitory peptides from wheat germ protein 显示文摘 | Junqiang Jia Haile Ma Weirui Zhao | 2010 | Food Chemistry2010，119，1： | 1 |
| 15 | The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germ protein 显示文摘 | JIA Junqiang MA Haile ZHAO Weirui | 2010 | Food Chem2010，119，1： | 1 |
| 16 | The use of ultrasound for enzymatic preparation of ACE inhibitory peptides from wheat germ protein显示文摘 | JIA Junqiang MA Haile ZHAO Weirui | 2010 | Food Chemistry2010，119，1： | 1 |
| 17 | Candidate Mycobacterium tuberculosis genes targeted by human microRNAs显示文摘Tuberculosis(TB)remains a major health issue,causing approximately three million deaths every year(Pelicic et al.,1998).Identified by Robert Koch in 1882,Mycobacterium tuberculosis(M.tuberculosis),the causative agent for tuberculosis,remains one of the most enigmatic bacteria.The interactions between M.tuberculosis and its environment have been extensively studied.However,our knowledge about potential M.tuberculosis-host interaction at the RNA level is still very limited.Here we present our preliminary finding that suggests possible interactions of human miRNAs with M.tuberculosis transcripts and speculate possible roles of human miRNAs in regulating macrophage-M.tuberculosis interactions. | WeiRui Guo Jiong-Tang Li Xiao Pan Liping Wei Jane Y.Wu | 2010 | Protein & Cell2010，1，5： | 1 |
| 18 | The use of ultrasound for enzymatic preparation of ACE-inhibitory peptides from wheat germ protein显示文摘 | Junqiang Jia Haile Ma Weirui Zhao | 2010 | Food Chemistry2010，119，1： | 1 |
| 19 | The use of ultrasound for enzymatic preparation of ACE inhibitory peptides from wheat germ protein显示文摘 | JIA Junqiang MA Haile ZHAO Weirui | | 0，，01： | 1 |
| 20 | Dynamics of Cohen-Grossberg Neural Network with Variable Coefficients and Time-varying Delays显示文摘 | Weirui Zhao | 2008 | Nonlinear Analysis: Real World Applications2008，，9： | 1 |
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