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| 1 | Inhibition of SARS-CoV-2 (previously 2019-nCoV) infection by a highly potent pan-coronavirus fusion inhibitor targeting its spike protein that harbors a high capacity to mediate membrane fusion显示文摘The recent outbreak of coronavirus disease(COVID-19)caused by SARS-CoV-2 infection in Wuhan,China has posed a serious threat to global public health.To develop specific anti-coronavirus therapeutics and prophylactics,the molecular mechanism that underlies viral infection must first be defined.Therefore,we herein established a SARS-CoV-2 spike(S)protein-mediated cell-cell fusion assay and found that SARS-CoV-2 showed a superior plasma membrane fusion capacity compared to that of SARS-CoV.We solved the X-ray crystal structure of six-helical bundle(6-HB)core of the HR1 and HR2 domains in the SARS-CoV-2 S protein S2 subunit revealing that several mutated amino acid residues in the HR1 domain may be associated with enhanced interactions with the HR2 domain.We previously developed a pan-coronavirus fusion inhibitor,EK1,which targeted the HR!domain and could inhibit infection by divergent human coronaviruses tested,including SARS-CoV and MERS-CoV.Here we generated a series of lipopeptides derived from EK1 and found that EK1C4 was the most potent fusion inhibitor against SARS-CoV-2 S protein-mediated membrane fusion and pseudovirus infection with IC50s of 1.3 and 15.8 nM,about 241-and 149-fold more potent than the original EK1 peptide,respectively.EK1C4 was also highly effective against membrane fusion and infection of other human coronavirus pseudoviruses tested,including SARS-CoV and MERS-CoV,as well as SARSr-CoVs,and potently inhibited the replication of 5 live human coronaviruses examined,including SARS-CoV-2.Intranasal application of EK1C4 before or after challenge with HCoV-OC43 protected mice from infection,suggesting that EK1C4 could be used for prevention and treatment of infection by the currently circulating SARS-CoV-2 and other emerging SARSr-CoVs. | Shuai Xia Meiqin Liu Chao Wang Wei Xu Qiaoshuai Lan Siliang Feng Feifei Qi Linlin Bao Lanying Du Shuwen Liu Chuan Qin Fei Sun Zhengli Shi Yun Zhu Shibo Jiang Lu Lu | 2020 | Cell Research2020，30，4： | 81 |
| 2 | HIV prevalence and influencing factors analysis of sentinel surveillance among men who have sex with men in China, 2003-2011显示文摘 | WANG Lan WANG Lu Jessie L.Norris LI Dong-min GUO Wei DING Zheng-wei WANG Ning | 2012 | Chinese Medical Journal2012，，11： | 57 |
| 3 | The Sunway TaihuLight supercomputer: system and applications显示文摘The Sunway Taihu Light supercomputer is the world's first system with a peak performance greater than 100 PFlops. In this paper, we provide a detailed introduction to the Taihu Light system. In contrast with other existing heterogeneous supercomputers, which include both CPU processors and PCIe-connected many-core accelerators(NVIDIA GPU or Intel Xeon Phi), the computing power of Taihu Light is provided by a homegrown many-core SW26010 CPU that includes both the management processing elements(MPEs)and computing processing elements(CPEs) in one chip. With 260 processing elements in one CPU, a single SW26010 provides a peak performance of over three TFlops. To alleviate the memory bandwidth bottleneck in most applications, each CPE comes with a scratch pad memory, which serves as a user-controlled cache. To support the parallelization of programs on the new many-core architecture, in addition to the basic C/C++and Fortran compilers, the system provides a customized Sunway Open ACC tool that supports the Open ACC2.0 syntax. This paper also reports our preliminary efforts on developing and optimizing applications on the Taihu Light system, focusing on key application domains, such as earth system modeling, ocean surface wave modeling, atomistic simulation, and phase-field simulation. | Haohuan FU Junfeng LIAO Jinzhe YANG Lanning WANG Zhenya SONG Xiaomeng HUANG Chao YANG Wei XUE Fangfang LIU Fangli QIAO Wei ZHAO Xunqiang YIN Chaofeng HOU Chenglong ZHANG Wei GE Jian ZHANG Yangang WANG Chunbo ZHOU Guangwen YANG | 2016 | Science China(Information Sciences)2016，59，7： | 45 |
| 4 | New Classification of Herlyn-Werner-Wunderlich Syndrome显示文摘 | Lan Zhu Na Chen Jia-Li Tong Wei Wang LeiZhang Jing-He Lang | 2015 | Chinese Medical Journal2015，，2： | 30 |
| 5 | Roles of Fas signaling pathway in vitamin E succinateinduced apoptosis in human gastric cancer SGC-7901 cells显示文摘AIM: To investigate the roles of Fas signaling pathway in vitamin E succinate-induced apoptosis in human gastric cancer SGC-7901 cells.METHODS: Human gastric cancer SGC-7901 cells were treated with VES at 5, 10, 20 mg@L-1, succinic acid and vitamin E as vehicle control and condition media only as untreated (UT) control. Apoptotic morphology was observed by DAPI staining. Western blot analysis was applied to measure the expression of Fas, FADD and caspase-8 proteins. After the cells were transiently transfected with Fas and FADD antisense oligonucleotides, respectively, caspase-8 activity was determined by flurometric method.RESULTS: The morphologically apoptotic changes were observed after VES treatment by DAPI staining. 23.7 % and 89.6 % apoptosis occurred after 24 h and 48 h of 20 mg@L-1 VES treatment, respectively. The protein levels of Fas, FADD and caspase-8 were evidently increased in a dose-dependent manner after 24 h of VES treatment. The blockage of Fas by transfection with Fas antisense oligonucleotides obviously inhibited the expression of FADD protein. After SGC-7901 cells were transfected with Fas and FADD antisense oligonucleotides, caspase-8 activity was obviously decreased (P<0.01), whereas Fas blocked more than FADD.CONCLUSION: VES-induced apoptosis in human gastric cancer SGC-7901 cells involves Fas signaling pathway including the interaction of Fas, FADD and caspase-8. | Kun Wu Yao Li Yan Zhao Yu-Juan Shah Wei Xia Lan Zhao,Department of Nutrition and Food Hygiene,Public Health School,Harbin Medical University,Harbin 150001,Heilongjiang Province,China Wei-Ping Yu,Genetics Institute,Texas University of USA,Austin,USA | 2002 | World Journal of Gastroenterology2002，8，6： | 30 |
| 6 | Fusion mechanism of 2019-nCoV and fusion inhibitors targeting HR1 domain in spike protein显示文摘Very recently,a novel coronavirus,2019-nCoV,emerged in Wuhan,China and then quickly spread worldwide,resulting in>17,388 confirmed cases and 361 deaths as of 3 February 2020,thus calling for the development of safe and effective therapeutics and prophylatics.1,2 Similar to severe acute respiratory syndrome(SARS)-CoV,2019-nCoV belongs to lineage B betacoronavirus,and it has the ability to utilize human angiotensin-converting enzyme 2(ACE2)as a receptor to infect human cells. | Shuai Xia Yun Zhu Meiqin Liu Qiaoshuai Lan Wei Xu Yanling Wu Tianlei Ying Shuwen Liu Zhengli Shi Shibo Jiang Lu Lu | 2020 | Cellular & Molecular Immunology2020，17，7： | 27 |
| 7 | Transumbilical single-port laparoscopic cholecystectomy: a case report显示文摘 | GUO Wei ZHANG Zhong-tao HAN Wei LI Jian-she JIN Lan LIU Jun ZHAO Xiao-mu WANG Yu | 2008 | Chinese Medical Journal2008，，23： | 26 |
| 8 | Perinatal complications and live-birth outcomes following assisted reproductive technology: a retrospective cohort study显示文摘Background:With the development of assisted reproductive technology(ART)and its increasing success rate in the mainland of China,more attention has been paid to the safety of ART.In this study,we explored the associations between conception by ART and pregnancy/perinatal complications,and neonatal outcomes compared with similar outcomes following spontaneous conception.Methods:This retrospective cohort study of pregnancies over a 3-year period(2013-2015)was performed at Beijing Obstetrics and Gynecology Hospital,Beijing,China.Subjects were divided into two groups:conception by ART(n=2256)or spontaneous conception(n=6768).According to different fertilization modes,the ART group was divided into in vitro fertilization(IVF,n=1873)and intracytoplasmic sperm injection(ICSI,n=383)subgroups.The ART group was also divided into two different embryo transfer methods;fresh embryo transfer(ET,n=1583)and frozen embryo transfer(FET,n=673)subgroups.Pregnancy complications,perinatal complications,and neonatal outcomes of the enrolled subjects were investigated and analyzed by univariate analysis and multivariate logistic regression.Results:After adjustment for maternal age,gravidity,parity,maternal education,smoking,alcohol consumption,and body mass index(BMI),pregnancies conceived by ART were associated with a significantly increased incidence of gestational diabetes mellitus(GDM;OR 1.88,95%CI 1.56-2.27),gestational hypertension(OR 2.18,95%CI 1.83-2.60),and intrahepatic cholestasis of pregnancy(ICP)(OR 2.79,95%CI 2.15-3.64),compared with spontaneous conception.These associations were similar for the singleton group.In the twin group,only the incidence of ICP was significantly higher than in controls.We found that pregnancies conceived by ART were associated with perinatal complications,including placental abruption(OR 2.14,95%CI 1.33-3.45),premature rupture of membranes(PROM;OR 1.24,95%CI 1.06-1.45),postpartum hemorrhage(OR 2.89,95%CI 2.33-3.59)and polyhydramnios(OR 2.01,95%CI 1.29-3.16).The singleton group had a similar result with placental abruption,but not with fetal membranes ruptures before labor and polyhydramnios.There were no significant differences in the incidence of these perinatal complications in the twin group.Some neonatal outcomes,including preterm labor(OR 4.29,95%CI 3.84-4.80)and low birth weight(OR 1.72,95%CI 1.42-2.08),were more likely to occur with singleton births after ART.However,there were no significant differences for these outcomes from twin pregnancies.Perinatal complications and neonatal outcomes were consistent between the IVF and ICSI subgroups.The FET and ET subgroups showed a similar increase in complications,except for the incidence of placental abruption.After taking into account the effects of parity,birth plurality and maternal age,the ART group still exhibited increased maternal and neonatal complications,although some differences narrowed or disappeared.Conclusions:This retrospective cohort study demonstrated that patients who underwent ART were at increased risk of several adverse pregnancy outcomes compared with women who conceived spontaneously.These complications may be attributed in part to the relatively high multiple pregnancy rate after ART.Elective single embryo transfer should be promoted in China to reduce the obstetrical risks of ART pregnancy.Singletons of ART pregnancy exhibited increased maternal and neonatal complications as well,suggesting that underlying infertility or other maternal or parental factors may contribute to the adverse outcomes. | Ling-Ling Lei Yong-Lian Lan Shu-Yu Wang Wei Feng Zhi-Jin Zhai | 2019 | Chinese Medical Journal2019，，20： | 24 |
| 9 | Field released transgenic papaya effect on soil microbial communities and enzyme activities显示文摘Soil properties, microbial communities and enzyme activities were stud ied in soil amended with replicase (RP)-transgenic or non-transgenic papaya unde r field conditions. Compared with non-transgenic papaya, significant differences (P<0.05) were observed in total nitrogen in soils grown with transgenic papaya. There were also significant differences (P<0.05) in the total number of colony forming units (CFUs) of bacteria, actinomycetes and fungi between soils amended with RP-transgenic plants and non-transgenic plants. Compared with non-transgen ic papaya, the total CFUs of bacteria, actinomycetes and fungi in soil with tran sgenic papaya increased by 0.43—1.1, 0.21—0.80 and 0.46—0.73 times respective ly. Significantly higher (P<0.05) CFUs of bacteria, actinomycetes and fungi res istant to kanamycin (Km) were obtained in soils with RP-transgenic papaya than t hose with non-transgenic papaya in all concentrations of Km. Higher resistance q uotients for Kmr (kanamycin resistant) bacteria, actinomycetes and fungi were fo und in soil planted with RP-transgenic papaya, and the resistance quotients for Kmr bacteria, actinomycetes and fungi in soils with transgenic papaya increased 1.6—4.46, 0.63—2.5 and 0.75—2.30 times. RP-transgenic papaya and non-transg enic papaya produced significantly different enzyme activities in arylsulfatase (5.4—5.9x), polyphenol oxidase (0.7—1.4x), invertase (0.5—0.79x), cellulase ( 0.23—0.35x) and phosphodiesterase (0.16—0.2x). The former three soil enzymes appeared to be more sensitive to the transgenic papaya than the others, and cou ld be useful parameters in assessing the effects of transgenic papaya. Transgen ic papaya could alter soil chemical properties, enzyme activities and microbial communities. | WEI Xiang-dong ZOU Hui-ling CHU Lee-min LIAO Bin YE Chang-min LAN Chong-yu | 2006 | Journal of Environmental Sciences2006，18，4： | 22 |
| 10 | RNA-Seq analysis of yak ovary: improving yak gene structure information and mining reproduction-related genes显示文摘RNA-Seq,a high-throughput(HT)sequencing technique,has been used effectively in large-scale transcriptomic studies,and is particularly useful for improving gene structure information and mining of new genes.In this study,RNA-Seq HT technology was employed to analyze the transcriptome of yak ovary.After Illumina-Solexa deep sequencing,26826516 clean reads with a total of 4828772880 bp were obtained from the ovary library.Alignment analysis showed that 16992 yak genes mapped to the yak genome and 3734 of these genes were involved in alternative splicing.Gene structure refinement analysis showed that7340 genes that were annotated in the yak genome could be extended at the 5′or 3′ends based on the alignments been the transcripts and the genome sequence.Novel transcript prediction analysis identified 6321 new transcripts with lengths ranging from 180 to 14884 bp,and 2267 of them were predicted to code proteins.BLAST analysis of the new transcripts showed that1200?4933 mapped to the non-redundant(nr),nucleotide(nt)and/or SwissProt sequence databases.Comparative statistical analysis of the new mapped transcripts showed that the majority of them were similar to genes in Bos taurus(41.4%),Bos grunniens mutus(33.0%),Ovis aries(6.3%),Homo sapiens(2.8%),Mus musculus(1.6%)and other species.Functional analysis showed that these expressed genes were involved in various Gene Ontology(GO)categories and Kyoto Encyclopedia of Genes and Genomes pathways.GO analysis of the new transcripts found that the largest proportion of them was associated with reproduction.The results of this study will provide a basis for describing the normal transcriptome map of yak ovary and for future studies on yak breeding performance.Moreover,the results confirmed that RNA-Seq HT technology is highly advantageous in improving gene structure information and mining of new genes,as well as in providing valuable data to expand the yak genome information. | LAN DaoLiang XIONG XianRong WEI YanLi XU Tong ZHONG JinCheng ZHI XiangDong WANG Yong LI Jian | 2014 | Science China(Life Sciences)2014，57，9： | 23 |
| 11 | Preliminary molecular epidemiology of the Staphylococcus aureus in lower respiratory tract infections: a multicenter study in China显示文摘 | LI De-zhi CHEN Yu-sheng YANG Jing-ping ZHANG Wei HU Cheng-ping LI Jia-shu MU Lan HU Ying-hui GENG Rong HU Ke CAI Shao-xi WAN Huan-ying WANG Qiu-yue WEI Li-ping DU Juan YU Qin ZHONG Xiao-ning WANG Rui-qin MA Jian-jun TIAN Gui-zhen WANG Si-qin GAO Zhan-cheng | 2011 | Chinese Medical Journal2011，，5： | 21 |
| 12 | Repurposing vitamin D for treatment of human malignancies via targeting tumor microenvironment显示文摘Tumor cells along with a small proportion of cancer stem cells exist in a stromal microenvironment consisting of vasculature, cancer-associated fibroblasts, immune cells and extracellular components.Recent epidemiological and clinical studies strongly support that vitamin D supplementation is associated with reduced cancer risk and favorable prognosis. Experimental results suggest that vitamin D not only suppresses cancer cells, but also regulates tumor microenvironment to facilitate tumor repression. In this review, we have outlined the current knowledge on epidemiological studies and clinical trials of vitamin D. Notably, wesummarized and discussed the anticancer action of vitamin D in cancer cells, cancer stem cells and stroma cells in tumor microenvironment, providing a better understanding of the role of vitamin D in cancer. We presently re-propose vitamin D to be a novel and economical anticancer agent. | Xu Wu Wei Hu Lan Lu Yueshui Zhao Yejiang Zhou Zhangang Xiao Lin Zhang Hanyu Zhang Xiaobing Li Wanping Li Shengpeng Wang Chi Hin Cho Jing Shen Mingxing Li | 2019 | Acta Pharmaceutica Sinica B2019，9，2： | 20 |
| 13 | 宣威肺癌分子流行病学研究:煤种、基因型与肺癌风险显示文摘背景与目的已有的研究证明:宣威是我国农村肺癌死亡率最高的地区,肺癌危险与室内燃烧烟煤产生的多环芳烃(polycyclic aromatic hydrocarbons,PAHs)有关,肺癌发病具有明显的地域性差别和家族聚集性。本研究从分子流行病学角度探索宣威肺癌风险的危险因素及与发病机理有关的基因型和燃煤类型。方法运用两个基于人群的病例对照研究,开展问卷调查,同时采集口腔细胞和痰等生物样品,提取DNA。应用PCR法检测GST超家族、AKR超家族和OGG1等基因型。通过Logistic回归分析煤种、基因型与肺癌风险的关联。结果与燃用无烟煤或木柴的研究对象相比,家用来宾烟煤的肺癌风险比数比(odds ratio,OR)值高达24.8,其次为龙潭(OR=11.6)、宝山(OR=6.0)、龙场(OR=4.1)、羊场(OR=3.8)等;燃用同种烟煤的男女肺癌风险相似。对于GSTM1缺失、AKR1C3(Ex1-70C>G)、OGG1(Ex6-315C>G)基因型,肺癌风险明显增高,且具统计学意义[OR(95%CI)分别为2.3(1.3-4.2)、1.8(1.0-3.5)和1.9(1.1-3.3)]。与家用烟煤量小且具GSTM1阳性的研究对象相比,用量大且GSTM1缺失的研究对象肺癌风险更高,女性OR为4.9(1.3-18.2),男性OR为2.7(1.0-7.4)。但对于AKR1C3(Ex1-70C>G)和OGG1(Ex6-315C>G),仅观察到女性肺癌风险的明显增高,OR分别为12.9(2.2-107.8)和5.7(1.1-34.2)。结论宣威不同煤种的肺癌风险有很大差异,但暴露于相同煤种的男性与女性肺癌风险相似。GSTM1缺失基因型与肺癌风险增高有关联。AKR1C3与OGG1这2种基因型与肺癌风险有关联,且在烟煤使用量高的女性中肺癌风险更高。 | 李继华 何俊 唐锐 Wei HU Qing LAN 何兴舟 李云 张云生 | 2015 | 中国肺癌杂志2015，18，1： | 19 |
| 14 | An exact solution of unsteady Couette flow of generalized second grade fluid显示文摘The fractional calculus approach is introduced into the rheological constitutive model of a generalized second grade fluid. A constitutive model with fractional derivative is developed for the generalized second grade fluid. Unsteady Couette flow of the generalized second grade fluid is studied by using the method of the discrete inverse Laplace transform and generalized Mittag-Leffler function. And then an exact solution is obtained for this problem with arbitrary fractional derivative. This provides a new analytical tool for the study of viscoelastic fluid mechanics. | TAN Wenchang XIAN Feng WEI Lan | 2002 | Chinese Science Bulletin2002，47，21： | 17 |
| 15 | Fine mapping and marker-assisted selection (MAS) of a low glutelin content gene in rice显示文摘Rice with low glutelin content is suitable as functional food for patients affected with diabetes and kidney failure. The fine mapping of the gene(s) responsible for low glutelin content will provide information regarding the distribution of glutelin related genes in rice genome and will generate markers for the selection of low glutelin rice varieties. Following an SDS-PAGE screen of rice germplasm from Taihu Valley of China, Japonica selection W3660 is identified to be a novel mutant characterized with low glutelin content. For fine mapping the mutant gene for low glutelin content, F2 and F3 populations were derived from a cross between W3660 and Jingrennuo. SDS-PAGE analysis of the total endosperm protein showed that the low glutelin content trait was controlled by a single dominant nuclear gene. Genetic mapping, using SSRs, located this gene to chromosome 2, in the region between SSR2-001/SSR2-004 and RM1358. The dis- tances of the two markers to the target gene were 1.1 cM and 3.8 cM respectively. By semi-quantitative RT-PCR analysis, the transcripts of GluB4/GluB5 genes located within the region do not change. However, GluB5 gene located proximal to SSR2-001/SSR2-004 was specifically reduced. SSR profiles of seven Japonica varieties were compared with that of W3660 for loci in the relevant genetic region. The markers SSR2-004 and RM1358 were used for marker- assisted selection. The selection efficiencies of SSR2-004 and RM1358 were 96.8% and 92.7% respectively. This provides a standard starting point for the breeding of low glutelin content rice varieties in China. | Yi Hua WANG Shi Jia LIU Su Lan JI Wen Wei ZHANG Chun Ming WANG Ling JIANG Jian Min WAN | 2005 | Cell Research2005，15，8： | 16 |
| 16 | Household air pollution and lung cancer in China:a review of studies in Xuanwei显示文摘Over half of the world's population is exposed to household air pollution from the burning of solid fuels at home. Household air pollution from solid fuel use is a leading risk factor for global disease and remains a major public health problem, especially in low- and mid-income countries. This is a particularly serious problem in China, where many people in rural areas still use coal for household heating and cooking. This review focuses on several decades of research carried out in Xuanwei County, Yunnan Province, where household coal use is a major source of household air pollution and where studies have linked household air pollution exposure to high rates of lung cancer. We conducted a series of case-control and cohort studies in Xuanwei to characterize the lung cancer risk in this population and the factors associated with it. We found lung cancer risk to vary substantially between different coal types, with a higher risk associated with smoky(i.e., bituminous) coal use compared to smokeless(i.e., anthracite) coal use. The installation of a chimney in homes resulted in a substantial reduction in lung cancer incidence and mortality. Overall, our research underscores the need among existing coal users to improve ventilation, use the least toxic fuel, and eventually move toward the use of cleaner fuels, such as gas and electricity. | Wei Jie Seow Wei Hu Roel Vermeulen H.Dean Hosgood III George S.Downward Robert S.Chapman Xingzhou He Bryan A.Bassig Christopher Kim Cuiju Wen Nathaniel Rothman Qing Lan | 2014 | Chinese Journal of Cancer2014，33，10： | 15 |
| 17 | Drug-resistant genes carried by Acinetobacter baumanii isolated from patients with lower respiratory tract infection显示文摘 | DAI Ning LI De-zhi CHEN Ji-chao CHEN Yu-sheng GENG Rong HU Ying-hui YANG Jing-ping DU Juan HU Cheng-ping ZHANG Wei LI Jia-shu YU Qin WAN Huan-ying MU Lan ZHONG Xiao-ning WEI Li-ping MA Jian-jun WANG Qiu-yue HU Ke TIAN Gui-zhen CAI Shao-xi WANG Rui-qin HE Bei WANG Si-qin WANG Zhan-wei ZHAO Su-rui GAO Zhan-cheng | 2010 | Chinese Medical Journal2010，，18： | 13 |
| 18 | Risk Factors for Lung Cancer in Non-smokers in Xuanwei County of China显示文摘Tobacco smoking and some types of occupational exposures have consistently been considered as important etiologic factors of lung cancer in industrial countrie.At the First World Conference of Lung Cancer(Wyndwer,1983),it was stressed that more attention should be paid to finding out the causes of lung cancer in non-smokers. In Xuanwei County,Yunnan Province,annual lung cancer death rate was 27.7per 100,000 in males,among China's highest,and 25.3per 100,000 in females,the China's highest.The remale's lung cancer death rate in Xuanwei County was even much higher than that of the same period in USA white women(ECACM,1979;Mulvihill,1976),Marked district variation in cancer mortality exists within xuanwei County.The county can be ivided into high-,medium-and low-mortality areas.Over90% of the population are farmers.The local residents traditionally burned three major kinds of fuels:“smoky” coal,“smokeless”coal and “wood”,for heating and cooking.The three lung cancer high mortality areas,including “Chengguan”,“Rongcheng”and “Laibin”communes,mainly burned the “smoky”!coal from Laibin smoky-coal mine.The fuel was burned in a shallow,unventiated fire pit in the floor of the dwelling.Fuel burning in shallow unventialated pits has resulted in high indoor air pollution levels.The concentrations of airborne particles(pm10) inside houses during smoky coal and wood combustion were very high.Indoor concentration of suspended particulates and dichloromethane extractable organics were 244.4mg/m^3 and 17.6mg/m^3 in burning of “smoky”coal;22.2mg/m^3,12.3mg/m^3 for burning wood;and 1.8mg/m^3,0.5mg/m^3 for burning of smokeless coals.Levels of individual polycyclic aromatic hydrocarbons.including benzo(a)pyrene,were generally highest during burning of smoky coal,intermediate in burning wood,and least in smokeless coal.Women did most household chores.which include starting fire and cooking.Less than 0.2% of females smoked tobacco.The population-based casecontrol study presented here is to evaluate the influence of factors on the occurrence of lung cancer in non-smoking women in Xuanwei County and to supplement the etiologic factors of lung cancer in Xuanwei County. | LAN QING, CHEN WEI, CHEN HONG, AND HE XING-ZHOUInstitute of Environmental Health and Engineering, Chinese Academy of Preventive Medicine 29 Nan Wei Road, Beijing 100050, China | 1993 | Biomedical and Environmental Sciences1993，6，2： | 13 |
| 19 | Two new terpenoids from fruits of Ziziphus jujuba显示文摘一新 ceanothane 类型 triterpene 和一新 sesquiterpene,和二,已知的 triterpenes 从 Ziziphus jujuba 米尔的水果被孤立。(Rhamnaceae ) 。基于光谱分析,二新混合物的结构作为 2 α 被阐明; -aldehydo-A(1)-norlup-20(29)-en-27,28-dioic 酸(zizyberanal 酸)(1 ) ,和 zizyberanone (2 ) 。当二已知的 triterpenes 作为 zizyberanalic 酸(3 ) 和 ursolic 酸(4 ) 被识别时。 | Sheng Guo Yu Ping Tang Jin Ao Duan Shu Lan Su An Wei Ding | 2009 | Chinese Chemical Letters2009，20，2： | 13 |
| 20 | PTEN and PDCD4 are Bona Fide Targets of microRNA-21 in Human Cholangiocarcinoma显示文摘Objective To investigate the expression profile of microRNA-21 in human cholangiocarcinoma tissues and to validate its bona fide targets in human cholangiocarcinoma cells. Methods The expression profile of microRNA-21 in human cholangiocarcinoma tissues and cholangiocarcinoma cell line, QBC939, was evaluated by using real-time PCR analysis. The bona fide targets of microRNA-21 were analyzed and confirmed by dual luciferase reporter gene assay and western blot, respectively. The expressional correlation of microRNA-21 and its targets was probed in human cholangiocarcinoma tissues by using real-time PCR, locked nucleic acid in situ hybridization (LNA-ISH), and immunohistochemistry analysis. Results Real-time PCR analysis revealed that microRNA-21 expression depicted a significant up-regulation in human cholangiocarcinoma tissues about 5.6-fold as compared to the matched normal bileduct tissues (P<0.05). The dual luciferase reporter gene assay revealed endogenous microRNA-21 in cholangiocarcinoma cell line, QBC939, inhibited the luciferase reporter activities of wild-type PTEN (P<0.01) and PDCD4 (P<0.05) and had no this effect on mutated PTEN and PDCD4. Moreover, loss of microRNA-21 function led to a significant increase of PTEN and PDCD4 protein levels in QBC939 cells. Elevated microRNA-21 levels were accompanied by marked reductions of PTEN and PDCD4 expression in the same cholangiocarcinoma tissue. Conclusion microRNA-21 expression is up-regulated in human cholangiocarcinoma and PTEN, PDCD4 are direct effectors of microRNA-21. | Chang-zheng Liu Wei Liu Yi Zheng Jin-mei Su Jing--jing Li Lan Yu Xiao-dong He Song-sen Chen | 2012 | Chinese Medical Sciences Journal2012，27，2： | 13 |
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