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| 1 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 2 | Catalytic enantioselective addition of diethylzinc to aldehydes: Application of a new bicyclic catalyst 显示文摘 | WALLBAUM S MARTENS J | 1993 | Tetrahedron: Asymmetry1993，4，4： | 1 |
| 3 | Optimization of the biological activity of combinatorial compound libraries by a genetic algorithm显示文摘 | Weber L Wallbaum S Broger C | 1995 | Angew Chem Int Ed1995，34，20： | 1 |
| 4 | The immediate early gene Ier2 promotes tumor cell motility and metastasis, and predicts poor survival of colorectal cancer patients 显示文摘 | Neeb A Wallbaum S Novac N | 2012 | Oncogene2012，31，33： | 1 |
| 5 | Some new zirconium compounds with transition elements 显示文摘 | Wallbaum H J | 1942 | Naturwissenschaften1942，30，910： | 1 |
| 6 | Using the Internet in Transport Logistics- The Example of a Track & Trace System显示文摘 | Kai Jakobs A1 Carsten Pils A1 Michael Wallbaum A1 | 2001 | Lecture Notes in Computer Science2001，，1： | 1 |
| 7 | Indicator based sustainability assessment tool for affordable housing construction technologies显示文摘 | Wallbaum H Ostermeyer Y Salzer C Escamilla E.Z | | 0，，05： | 1 |
| 8 | Enantioselective addition of diethylzinc to aromatic aldehydes catalysed by chiral ligands derived from L-hydroxyproline 显示文摘 | Mehler T Martens J Wallbaum S | 1993 | Synth Commun1993，23，19： | 1 |
| 9 | Optimierung der bilogischen aktivitaet yon kombinatorischen verbindungsbibliotheken durch einen genetischen algorithmus 显示文摘 | Wallbaum S Broger C | 1995 | Angew Chem1995，，107： | 1 |
| 10 | Asymmetric syntheses with chiral oxazaborolidines 显示文摘 | Wallbaum S Martens J | 1992 | Tetrahedron: Asymmetry1992，3，12： | 1 |
| 11 | Stahl im Vergleich -Verfahren,Ressourceneffizienz,Recycling,Umwelt显示文摘 | Ritthoff M Meaen T Wallbaum H | 2004 | Stahl und Eisen2004，，7： | 1 |
| 12 | Asymmetric syntheses with chiral oxazaborolidines显示文摘 | Wallbaum S Martens J | | 0，，： | 1 |
| 13 | 可持续建筑部门的理念与手段显示文摘本文概览了伍珀塔尔研究所为建筑部门确立可持续性目标,以及为实现这些目标开创途径所采取的多种方法.资源生产力是覆盖建筑物整个生命周期的考虑(MIPS).为了迈向更大的可持续性,COMPASS理念把单一公司或工业部门的环境、经济和社会各方面因素综合考虑.这些方法中的每一种都有所作为,再根据不同类型的研究,为公司和政策制定者提出建议.多边利益相关者程序可以用来推动建筑部门的全面可持续发展,最终把相关的理念明确地推进到微型和中等级别上. | Holger Wallbaum Claudia Buerkin | 2004 | 生态毒理学报2004，，2： | 0 |
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