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| 1 | Antifibrogenic effects of vitamin D derivatives on mouse pancreatic stellate cells显示文摘AIM To study the molecular effects of three different D-vitamins, vitamin D2, vitamin D3 and calcipotriol, in pancreatic stellate cells(PSCs).METHODS Quiescent PSCs were isolated from mouse pancreas and activated in vitro by seeding on plastic surfaces. The cells were exposed to D-vitamins as primary cultures(early-activated PSCs) and upon re-culturing(fullyactivated cells). Exhibition of vitamin A-containing lipid droplets was visualized by oil-red staining. Expression of α-smooth muscle actin(α-SMA), a marker of PSC activation, was monitored by immunofluorescence and immunoblot analysis. The rate of DNA synthesis was quantified by 5-bromo-2'-deoxyuridine(Brd U) incorporation assays. Real-time PCR was employed to monitor gene expression, and protein levels of interleukin-6(IL-6) were measured by ELISA. Uptake of proline was determined using 18 F-proline.RESULTS Sustained culture of originally quiescent PSCs inducedcell proliferation, loss of lipid droplets and exhibition of stress fibers, indicating cell activation. When added to PSCs in primary culture, all three D-vitamins diminished expression of α-SMA(to 32%-39% of the level of control cells; P < 0.05) and increased the storage of lipids(scores from 1.97-2.15 on a scale from 0-3; controls: 1.49; P < 0.05). No such effects were observed when D-vitamins were added to fully-activated cells, while incorporation of Brd U remained unaffected under both experimental conditions. Treatment of re-cultured PSCs with D-vitamins was associated with lower expression of IL-6(-42% to-49%; P < 0.05; also confirmed at the protein level) and increased expression of the vitamin D receptor gene(209%-321% vs controls; P < 0.05). There was no effect of D-vitamins on the expression of transforming growth factor-β1 and collagen type 1(chain α1). The lowest uptake of proline, a main component of collagen, was observed in calcipotriol-treated PSCs.CONCLUSION The three D-vitamins inhibit, with similar efficiencies, activation of PSCs in vitro, but cannot reverse the phenotype once the cells are fully activated. | Peter Wallbaum Sarah Rohde Luise Ehlers Falko Lange Alexander Hohn Carina Bergner Sarah Marie Schwarzenbock Bernd Joachim Krause Robert Jaster | 2018 | World Journal of Gastroenterology2018，24，2： | 3 |
| 2 | Single lung transplantation for adult respiratory distress syndrome after paraquat piosoning 显示文摘 | Marc Licker Alexander Schweizer Laurent Hohn | 1998 | Thorax1998，53，： | 1 |
| 3 | TRII2, a trithothecene efflux pump from Fusarium sporotrichiodes: gene isolation and expression in yeast显示文摘 | Alexander N J McCormick S P Hohn T M | 1999 | Molecular & General Genetics1999，261，97： | 1 |
| 4 | The identification of the Saccharomyces cerevisiae gene AYT1(ORF-YLL063c)enco-ding an acetyltransferase显示文摘 | Alexander NJ McCormick SP Hohn TM | | 0，，16： | 1 |
| 5 | Bubble-wall interactions in a vertical gas-liquid flow:Bouncing, sliding and bubble deformations显示文摘 | Alexander Zaruba^a ^* Dirk Lueas^a Horst-Miehael Prasser^b Thorns Hohne^a | 2007 | Chemical Engineering Science2007，62，6： | 1 |
| 6 | TRI12,a trichothecene efflux pump from Fusarium sporotrichioides:gene isolation and expression in yeast显示文摘 | Alexander N J McCormick S P Hohn T M | 1999 | Molecular General Genetics1999，261，： | 1 |
| 7 | TRI12, a trichothecene efflux pump from Fusarium sporotrichioides: gene isolation and expression in yeast显示文摘 | N. J. Alexander S. P. McCormick T. M. Hohn | 1999 | MGG - Molecular and General Genetics1999，，6： | 1 |
| 8 | The TRⅢ gene of Fusirium sporotrichioides encodes a cytochrome P-450 monooxygenase required for C-15 hydroxylation in trichothecene biosynthesis显示文摘 | Alexander N J Hohn TM Mcormick S P | | 0，，： | 1 |
| 9 | Engineering deoxynivalenol metabolism in wheat through the expression of a fungal trichothecene acetyltransferase gene显示文摘 | P. Okubara A. Blechl S. McCormick N. Alexander R. Dill-Macky T. Hohn | 2002 | Theoretical and Applied Genetics2002，，1： | 1 |
| 10 | Tri12, a trithothecene efflux pump from Fusarium sporotrichioides : gene isolation and expression in yeast 显示文摘 | Alexander N J McCormick S P Hohn T M | 1999 | Mol Gen Genet1999，261，： | 1 |
| 11 | Single lung transplantation for adult respiratory distress syndrome after paraquat poisoning 显示文摘 | Marc Licker Alexander Schweizer Lanrent Hohn | 1998 | Thorax1998，53，7： | 1 |
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