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| 1 | PIV measurement on internal instantaneous flows of a centrifugal pump显示文摘In this paper,the internal flow field in a centrifugal pump working at the design flow rate operating condition has been measured using the particle image velocimetry(PIV)technique with the fluorescent particles and the index-matched fluid technology.The index-marching fluid with the same refractive index as the transparent material has been prepared and applied in the present test of pump with geometrical complex walls.The comparison between velocity distributions of PIV results both with and without fluorescent particles,as well as with and without index-marching fluid are conducted to find the differences.The DES(Detached Eddy Simulation)has been employed to calculate the three-dimensional unsteady turbulent flow in the pump to examine and to certify the PIV measurement results.The DES results of instantaneous flow velocity fields agree with PIV test results with fluorescent particles and index-marching fluid.It is necessary to perform the PIV measurement of pumps with fluorescent seeds and index-marching fluid in order to get exact results.The experimental results show the distributions of velocity,steamlines,and the principal Reynolds normal stress(PRNS)and the principal Reynolds shear stress(PRSS). | WU YuLin LIU ShuHong YUAN HuiJing SHAO Jie | 2011 | Science China(Technological Sciences)2011，54，2： | 20 |
| 2 | An optimization method on runner blades in bulb turbine based on CFD analysis显示文摘In this paper an optimization method of the runner blades in a bulb turbine based on CFD analysis is proposed.In the method the main scales of the turbine including guide vane,runner and draft are maintained.Only the runner blades are modified based on the present method.In the optimization method the runner blade is expressed by spline surface with a gather of coordinate points.The B-spline curve is used to keep the modified blades smooth.In order to make the blade optimization simple and ef- ficient,one of the coordinates is fixed and only the angles of the points are changed according to different modification purposes.Three main optimization principles based on flow diagnosis are presented here.These three principles are all based on the CFD analysis of the internal flow in bulb turbine.For the purpose of method verification,the optimization method is used in a model bulb turbine.A three dimensional steady turbulent computation is carried out through the whole passage including the bulb body,guide vanes,runner and draft tube of the bulb turbine under seven different work conditions.An SST k-ωturbulence model is used during the CFD analysis and the performance of the turbine can be achieved.The runner blade is optimized according to the three optimization principles based on flow diagnosis.The CFD analysis is conducted again on the optimized turbine and another modification is needed if the new turbine can’t satisfy the required performance.Comparison of the computational results between the original turbine and an optimized one indicates that the optimization method is practical and does improve the performance of the bulb turbine. | YANG Wei WU YuLin LIU ShuHong | 2011 | Science China(Technological Sciences)2011，54，2： | 15 |
| 3 | Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application显示文摘The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application.Early angiogenesis is important for scaffold survival.It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties.In this study,a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold.For osseointegration and osteogenesis analysis,in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation,adhesion,extracellular matrix(ECM)mineralization and ALP activity compared with bare Ti6Al4V cocultivation.Additionally,MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesisrelated genes expression.In vivo studies including fluorochrome labeling,micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation.For angiogenesis studies,magnesium-coated Ti6Al4V improve HUVECs proliferation,adhesion,tube formation,wound-healing and Transwell abilities.HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes(HIF-1αand VEGF)expression.Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation.This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications. | Peng Gao Bo Fan Xiaoming Yu Wenwen Liu Jie Wu Lei Shi Di Yang Lili Tan Peng Wan Yulin Hao Shujun Li Wentao Hou Ke Yang Xiaokang Li Zheng Guo | 2020 | Bioactive Materials2020，5，3： | 15 |
| 4 | Discovery of mantle and lower crust xenoliths from early Cretaceous volcanic rocks of southwestern Tianshan,Xinjiang显示文摘In Tuoyun area of southwestern Tianshan, mantle and lower crust xenoliths are present In the volcanic rocks with ages of 101-123 Ma. Mantle xenoliths include mineral megacrysts such as kaersutite and pargasite, feldspar, biotite, and rare pyroxene and rock fragments such as perodotite, pyroxenite, amphibolite, and rare glimmerite. Lower crust xenoliths are mainly banded and massive granulite. The volcanic rocks were produced by within-plate magmatism. Occurrence of hydrous and volatile mineral megacrysts, amphibolite, and some pyroxenite containing hydrous and volatile minerals indicates that mantle metasomatism was intense. Undoubtedly, this discovery is very important to understanding of the crust-mantle structure and geodynamic background in depth in southwestern Tianshan and geological correlation with adjacent regions. | Baofu Han Xuechao Wang Guoqi He Tairan Wu Maosong Li Yulin Liu Shiguang Wang | 1999 | Chinese Science Bulletin1999，44，12： | 11 |
| 5 | Face recognition system based on CNN and LBP features for classifier optimization and fusion显示文摘Face recognition has been a hot-topic in the field of pattern recognition where feature extraction and classification play an important role. However,convolutional neural network( CNN) and local binary pattern( LBP) can only extract single features of facial images,and fail to select the optimal classifier. To deal with the problem of classifier parameter optimization,two structures based on the support vector machine( SVM) optimized by artificial bee colony( ABC) algorithm are proposed to classify CNN and LBP features separately. In order to solve the single feature problem,a fusion system based on CNN and LBP features is proposed. The facial features can be better represented by extracting and fusing the global and local information of face images. We achieve the goal by fusing the outputs of feature classifiers. Explicit experimental results on Olivetti Research Laboratory( ORL) and face recognition technology( FERET) databases show the superiority of the proposed approaches. | Wu Yulin Jiang Mingyan | 2018 | The Journal of China Universities of Posts and Telecommunications2018，25，1： | 10 |
| 6 | Cotton bollworm resistance to Bt transgenic cotton: A case analysis显示文摘Cotton bollworm (Helicoverpa armigera) is one of the most serious insect pests of cotton. Transgenic cotton expressing Cry toxins derived from a soil bacterium, Bacillus thuringiensis (Bt), has been produced to target this pest. Bt cotton has been widely planted around the world, and this has resulted in efficient control of bollworm populations with reduced use of synthetic insecticides. However, evolution of resistance by this pest threatens the continued success of Bt cotton. To date, no field populations of bollworm have evolved significant levels of resistance; however, several laboratory-selected Cry-resistant strains of H. armigera have been obtained, which suggests that bollworm has the capacity to evolve resistance to Bt. The development of resistance to Bt is of great concern, and there is a vast body of research in this area aimed at ensuring the continued success of Bt cotton. Here, we review studies on the evolution of Bt resistance in H. armigera, focusing on the biochemical and molecular basis of Bt resistance. We also discuss resistance management strategies, and monitoring programs implemented in China, Australia, and India. | LIU ChenXi, LI YunHe, GAO YuLin, NING ChangMing & WU KongMing State Key Laboratory of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science, Beijing 100193, China | 2010 | Science China(Life Sciences)2010，53，8： | 10 |
| 7 | An improved large eddy simulation of two-phase flows in a pump impeller显示文摘用动态秒顺序 sub-grid-scale (SGS ) 的改进大旋涡模拟压力模型被开发了为稠密的狂暴的粒子液体的管理方程建模在一个旋转坐标系统的二阶段的流动,和连续性被一个团加权的方法保存解决过滤管理方程。在当前的秒顺序 SGS 模型, SGS 压力是解决的紧张率和旋转率张肌的功能,并且模型参数从维的一致性和紧张率和旋转率张肌的 invariants 被获得。在数字计算,有限的卷方法习惯于 discretize 有一个蹒跚的格子系统的管理方程。SIMPLEC 算法被申请管理方程的 discretized 的答案。适合身体的坐标被用来在复杂几何学模仿二阶段的流动。最后秒顺序动态 SGS 模型成功地被使用模仿稠密的狂暴的粒子液体在离心的 impeller 的二阶段的流动。预言的压力和速度分布在对试验性的结果的好同意。 | Xuelin Tang Fujun Wang Yulin Wu | 2007 | Acta Mechanica Sinica2007，23，6： | 10 |
| 8 | Numerical simulation of pressure fluctuation in Kaplan turbine显示文摘As it is almost impossible to carry out the prototype hydro-turbine experiment be- fore the power plant is built up, rational prediction of pressure fluctuations in the prototype turbine is very important at the design stage. From this viewpoint, we at first treated the unsteady turbulent flow computation based on the modified RNG k-ε turbulence model through the whole flow passage to simulate the pressure fluctuation in a model turbine. Since fair agreement was recognized between the numerical results and the experimental data, this numerical method was applied to simulate the pressure fluctuations in the prototype turbine. From the comparison of them with the model turbine results, it is seen that their qualitative trend of pres- sure fluctuations are similar, but an appreciable difference is observed between the amplitudes of pressure fluctuation of the prototype turbine and that of the model turbine. Though the present findings may be explained by the effect of Reynolds number, further studies are expected for quantitative interpretation. We paid atten- tion to the interaction between the fluid and turbine structure. Adopting a weak fluid-solid coupling method, we studied the pressure fluctuation in the prototype turbine to clarify how the elastic behavior of runner blades influenced the charac- teristics of pressure fluctuation. | LIU ShuHong SHAO Jie WU ShangFeng WU YuLin | 2008 | Science China(Technological Sciences)2008，51，8： | 9 |
| 9 | Effects of Sini San used alone and in combination with fluoxetine on central and peripheral 5-HT levels in a rat model of depression显示文摘OBJECTIVE: To investigate the effects of Sini San and fluoxetine on the levels of central and peripheral 5-HT in a rat model of depression, and provide new insight into the treatment of depression with integrated Chinese-Western Medicine.METHODS: A rat model of depression was established by chronic mild stress(CMS). Model rats received either Sini San, fluoxetine, a combination of the two drugs, or no drug treatment. Healthy naive rats were used as controls. Open field and sucrose preference tests were used to assess depression-like behavior. ELISA and immunohistochemistry were used to determine central and peripheral levels of 5-HT.RESULTS: In the group with no drug treatment,central 5-HT expression decreased while peripheral5-HT concentrations increased as CMS continued.Four weeks after CMS, Sini San alone was less effective in reducing depression-like behavior than fluoxetine alone or in combination with Sini San,but combined use was more effective than fluoxetine alone. Eight weeks after CMS, Sini San alone or in combination with fluoxetine was more effective in reducing depression-like behavior than fluoxetine alone. Furthermore Sini San and fluoxetine used alone or in combination notably increased central 5-HT expression and decreased peripheral 5-HT levels in the rat model.CONCLUSION:The results of the present study indicate that there is a synergistic action between the two medicines in the treatment of depression. Sini San exhibited a relatively long lag before its effects were observed; however, by eight weeks the Traditional Chinese Medicine appeared at least as effective as fluoxetine. We suggest that Sini San can replace fluoxetine in the later stages of depression treatment to minimize side effects observed with long-term fluoxetine administration. | Yubo Li Yuxiu Sun Xueling Ma Xiaoxing Xue Wenting Zhang Zhiqian Wu Yulin Ouyang Jianxin Chen Weiming Wang Shuzhen Guo Wei Wang | 2013 | Journal of Traditional Chinese Medicine2013，33，5： | 6 |
| 10 | Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO_(3)/TC4 using low-intensity pulsed ultrasound显示文摘Developing bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research.Barium titanate(BT)is an electroactive material that can generate electrical signals in response to applied mechanical forces.In this study,a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy,forming a BT/TC4 material,and low-intensity pulsed ultrasound(LIPUS)was then applied as a mechanical stimulus.The combined effects on the biological responses of MC3T3-E1 cells were investigated.Results of scanning electron microscopy,energy-dispersive X-ray spectroscopy,and X-ray diffraction showed that an uniform nanospheres-shaped BT coating was formed on TC4 substrate.Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d_(33)of 0.42 pC/N.Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10μA/cm^(2).In vitro,the greatest osteogenesis(cell adhesion,proliferation,and osteogenic differentiation)was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS.Furthermore,the intracellular calcium ion concentration increased in these cells,possibly because opening of the L-type calcium ion channels was promoted and expression of the Ca_(V)1.2 protein was increased.Therefore,the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis,rending it a potential treatment for early stage formation of reliable bone-implant contact. | Kunzhan Cai Yilai Jiao Quan Quan Yulin Hao Jie Liu Lin Wu | 2021 | Bioactive Materials2021，6，11： | 6 |
| 11 | STUDY ON FLOWS THROUGH CENTRIFUGAL PUMP IMPELLER BY TURBULENT SIMULATION显示文摘A finite volume numerical method is presented for simulating internal tur-bulent flows on the blade-to-blade surface through a centrifugal pump impeller, in which, a mesh in body-fitted coordinates is generated for the computation. In detail, the SIM-PLEC algorithm is utilized for solving governing equations of incompressible viscous/turbulent flows through the pump impeller. The K-ε turbulence model is adopted to describe the turbulent flow process. Some worthwhile simulated results are presented. | Dai, Jiang Wu, Yulin Cao, Shuliang | 1997 | Journal of Hydrodynamics1997，9，1： | 5 |
| 12 | Precise time frequency synchronization technology for bistatic radar显示文摘A complete method of synchronization technology of bistatic radar using global position system (GPS) is presented. The pulse per second signal (1PPS) is elaborately modified to increase the time synchronization precision and keep loop locking. A very high time synchronization precision is achieved. Using the modified 1PPS to discipline the local OCXO, the reference frequency signal achieves both high long term stability (LTS) and short term stability (STS) properties. An algorithm, named phase abrupt change CFAR is presented to restrain the 1PPS phase abrupt change and keep loop locking. The experimental results indicate that this time and frequency synchronization method is effective and the time synchronization precision of the synchronization system can be improved from ±100 ns to ±25 ns. In addition, the phase noise is improved to 20 dB. | Huang Yulin Yang Jianyu Wu Junjie Xiong Jintao | 2008 | Journal of Systems Engineering and Electronics2008，19，5： | 4 |
| 13 | Depression accelerates gastric cancer invasion and metastasis by inducing a neuroendocrine phenotype via the catecholamine/β_(2)-AR/MACC1 axis显示文摘Background:Depression is a common,easily ignored,accompanied disease of gastric cancer(GC)patients and is often observed with elevated plasma catecholamine levels.Depression frequently promotes GC progression and leads to poor clinical outcomes;however,the molecular mechanisms underlying depression-induced GC progression remain poorly understood.We aimed to study the effects of depression on GC progression and explore possible mechanisms mediating the action of depression-associated catecholamines on GC.Methods:Depression states of GC patients were graded using the Patient Health Questionnaire-9,and plasma catecholamine levels were examined by high performance liquid chromatography coupled with tandem mass spectrometry.Migrative and invasive GC cells were examined using transwell assays,and metastatic GC niches were imaged using bioluminescence technology in a depression mouse model established with chronic unpredictable mild stress.Mouse depression-like behaviors were assessed through sucrose preference,forced swimming,and tail suspension tests.Characteristics of the neuroendocrine phenotype were observed via RT-PCR,Western blotting,flow cytometry,and transmission electron microscopy.Results:Fifty-one GC patients(age:53.61±1.79 years;cancer duration:3.71±0.33 months;depression duration:2.37±0.38 months;male-to-female ratio:1.55:1)were enrolled in the study.Depression grade was significantly higher in GC patients showing higher plasma levels of catecholamines(epinephrine:P=0.018;noradrenaline:P=0.009),higher oncogene metastasis-associated in colon cancer-1(MACC1)level(P=0.018),and metastasis(P<0.001).Further,depression-associated catecholamine specifically bound to the beta-2 adrenergic receptor(β_(2)-AR)and upregulated MACC1 expression,and thus promoting neuroendocrine phenotypic transformation through direct binding betweenMACC1 and synaptophysin.Eventually,the neuroendocrine phenotypic transformation accelerated GC invasion in vitro and metastasis in vivo.However,β_(2)-AR antagonist ICI-118,551 or MACC1 silencing effectively blocked the catecholamineinduced neuroendocrine phenotypic transformation and eliminated depressionenhanced GC migration and invasion.Moreover,β_(2)-AR blocking or MACC1 silencing prevented GC metastasis attributed to a neuroendocrine phenotype in a depression mouse model.Conclusions:Catecholamine-induced neuroendocrine phenotypes of GC cells led to depression-accelerated GC invasion and metastasis via the β_(2)-AR/MACC1 axis,while β_(2)-AR antagonist or MACC1 silencing could reverse it,showing promising potential therapeutic strategies for improving the outcome of GC patients with comorbid depression. | Changqie Pan Jianhua Wu Siting Zheng Huiying Sun Yisheng Fang Zhenhua Huang Min Shi Li Liang Jianping Bin Yulin Liao Jinzhang Chen Wangjun Liao | 2021 | Cancer Communications2021，41，10： | 4 |
| 14 | Methylglyoxal increases dopamine level and leads to oxidative stress in SH-SY5Y cells显示文摘越来越多的研究建议了 type-2 糖尿病导致的 methylglyoxal (MGO ) 与 Parkinson 的疾病(PD ) 有关。然而,很少对分子的机制被知道。在这研究,我们在 neuroblastoma SH-SY5Y 房间探索了 MGO 毒性。MGO 的 Neurotoxicity 被 mitochondrial 膜潜力, malondialdehyde,和 methylthiazoletetrazolium 试金测量。多巴胺, 3,4-dihydroxyphenylacetic 酸(DOPAC ) ,和 1-methyl-4-phenyl-1,2,3,4-tetrahydroisoquinoline (salsolinol ) 的层次被液体 chromatographymass spectrometry/mass spectrometry 检测。酷氨酸 hydroxylase (TH ) 和多巴胺 transporter (DAT ) 的表情被反向的 transcriptase 聚合酶链反应和西方的污点分析检测。当多巴胺, DOPAC,和内长的神经毒素 salsolinol 的层次也增加了时,结果证明 MGO 在 SH-SY5Y neuroblastoma 房间在 TH 和 DAT 表情导致了增加。Aminoguanidine (AG ) 是 MGO 的一个禁止者。AG 能减少,这被发现反应的氧种类(ROS ) 水平由 MGO 导致了,但是不能禁止 TH, DAT 和多巴胺的增加。多巴胺, DOPAC 和 salsolinol 层次的增加能导致高 ROS 和 mitochondrial 损坏。当 MGO 在糖尿病的功课期间被增加时,这研究建议多巴胺引起的 ROS 能贡献 dopaminergic 神经原的损坏。 | Bingjie Xie Fankai Lin Lei Peng Kaleem Ullah Hanyan Wu Hong Qing Yulin Deng | 2014 | Acta Biochimica et Biophysica Sinica2014，46，11： | 4 |
| 15 | Copy number variations of HLA-DRB5 is associated with systemic lupus erythematosus risk in Chinese Han population显示文摘全身的豺狼座 erythematosus (SLE ) 是复合基因,全身的、自体免疫的疾病。拷贝数字变体(CNVS ) 被发现了与很多复杂混乱被联系。我们拿了当前的学习在中国汉人口探索在 genomic CNVS 和 SLE 之间的潜在的协会。在发现阶段,七个 SLE 病人在屏蔽测试与高密度的比较 genomic 杂交 microarrays 被检查因为 SLE 联系了 CNVS。在确认阶段,然后, 135 个 SLE 病人和 219 个匹配的健康题目被 AccuCopy 技术为基因 HLA-DRB5 的 CNVS 调查。量的聚合酶链反应被执行在 SLE 病人决定拷贝数字(CN ) 和 HLA-DRB5 的 mRNA 水平。尽管在在 SLE 病人的 CN 删除组和 CN 正常的组之间的 HLA-DRB5 的 mRNA 水平不是统计上积极的( P = 0.46 ),我们的结果仍然比在健康控制在 SLE 病人显示出 HLA-DRB5 的更多的 CN ( P = 3.98 業慮瑮愠摮爠'酞棙M敶洠摯汥漠?偔?挠摯湯?′敷敲瀠牥潦浲摥?敲灳'虡N敶祬?剏?ㄠ????‥??ㄠ???劳?倠吗?? | Lijun Wu Shicheng Guo Dongqin Yang Yanyun Ma Hengdong Ji Yulin Chen Jun Zhang Yi Wang Li Jin Jiucun Wang Jie Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，2： | 3 |
| 16 | Silica supply and diatom blooms in the Jiaozhou Bay, China显示文摘The variations in nutrients(molar ratios) and chlorophyll a in the Jiaozhou Bay were examined before and after a diatom bloom in a period that lasted from November 2003 to March 2004. Negative relationships between nutrient concentrations, Si/P, Si/N ratios and chlorophyll a content were found during the bloom, which reflected the relationship between nutrient concentrations, phytoplankton biomass and growth. Large increase in nutrient concentrations, particularly SiO_3-Si after the late autumn, is one of major reasons inducing the diatom bloom in winter, and the bloom was finally controlled due to SiO_3-Si depletion by phytoplankton. The bloom was mainly controlled by SiO_3-Si. Before 1998, relatively low level of SiO_3-Si kept ecological balance of eutrophication waters in the Jiaozhou Bay. In recent years, however, human activities have increased SiO_3-Si concentration, which is likely one of the primary cause for the increased diatom blooms in the Jiaozhou Bay. Therefore, it is necessary to control SiO_3-Si concentration in the Jiaozhou Bay. | SHEN Zhiliang YAO Yun WU Yulin | 2016 | Acta Oceanologica Sinica2016，35，10： | 3 |
| 17 | Carbon inputs regulate the temperature sensitivity of soil respiration in temperate forests显示文摘Litter and root activities may alter the temperature sensitivity(Q_(10))of soil respiration.However,existing studies have not provided a comprehensive understanding of the effects of litter and root carbon inputs on the Q_(10)of soil respiration in different seasons.In this study,we used the trench method under in situ conditions to measure the total soil respiration(R_(total)),litter-removed soil respiration(R_(no-litter)),root-removed soil respiration(R_(no-root)),and the decomposition of soil organic matter(i.e.,both litter and root removal;R_(SOM))in different seasons of pioneer(Populus davidiana Dode)and climax(Quercus liaotungensis Mary)forests on the Loess Plateau,China.Soil temperature,soil moisture,litter biomass,fine root biomass,litter carbon,and root carbon were analyzed to obtain the drive mechanism of the Q_(10)of soil respiration in the two forests.The results showed that the Q_(10)of soil respiration exhibited seasonality,and the Q_(10)of soil respiration was higher in summer.The litter enhanced the Q_(10)of soil respiration considerably more than the root did.Soil temperature,soil moisture,fine root biomass,and litter carbon were the main factors used to predict the Q_(10)of different soil respiration components.These findings indicated that factors affecting the Q_(10)of soil respiration highly depended on soil temperature and soil moisture as well as related litter and root traits in the two forests,which can improve our understanding of soil carbon–climate feedback in global warming.The results of this study can provide reference for exploring soil respiration under temperate forest restoration. | LIU Yulin LI Jiwei HAI Xuying WU Jianzhao DONG Lingbo PAN Yingjie SHANGGUAN Zhouping WANG Kaibo DENG Lei | 2022 | Journal of Arid Land2022，14，9： | 3 |
| 18 | Multi-omic characterization of genome-wide abnormal DNA methylation reveals diagnostic and prognostic markers for esophageal squamous-cell carcinoma显示文摘This study investigates aberrant DNA methylations as potential diagnosis and prognosis markers for esophageal squamous-cell carcinoma(ESCC),which if diagnosed at advanced stages has<30%five-year survival rate.Comparing genome-wide methylation sites of 91 ESCC and matched adjacent normal tissues,we identified 35,577 differentially methylated CpG sites(DMCs)and characterized their distribution patterns. | Yiyi Xi Yuan Lin Wenjia Guo Xinyu Wang Hengqiang Zhao Chuanwang Miao Weiling Liu Yachen Liu Tianyuan Liu Yingying Luo Wenyi Fan Ai Lin Yamei Chen Yanxia Sun Yulin Ma Xiangjie Niu Ce Zhong Wen Tan Meng Zhou Jianzhong Su Chen Wu Dongxin Lin | 2022 | Signal Transduction and Targeted Therapy2022，7，3： | 2 |
| 19 | Numerical investigation of the hump characteristic of a pump–turbine based on an improved cavitation model显示文摘 | Jintao Liu Shuhong Liu Yulin Wu Lei Jiao Leqin Wang Yuekun Sun | 2012 | Computers and Fluids2012，，： | 2 |
| 20 | The interaction between polyphyllin I and SQLE protein induces hepatotoxicity through SREBP-2/HMGCR/SQLE/LSS pathway显示文摘Polyphyllin I(PPI)and polyphyllin II(PII)are the main active substances in the Paris polyphylla.However,liver toxicity of these compounds has impeded their clinical application and the potential hepatotoxicity mechanisms remain to be elucidated.In this work,we found that PPI and PII exposure could induce significant hepatotoxicity in human liver cell line L-02 and zebrafish in a dose-dependent manner.The results of the proteomic analysis in L-02 cells and transcriptome in zebrafish indicated that the hepatotoxicity of PPI and PII was associated with the cholesterol biosynthetic pathway disorders,which were alleviated by the cholesterol biosynthesis inhibitor lovastatin.Additionally,3-hydroxy-3-methy-lglutaryl CoA reductase(HMGCR)and squalene epoxidase(SQLE),the two rate-limiting enzymes in the cholesterol synthesis,selected as the potential targets,were confirmed by the molecular docking,the overexpression,and knockdown of HMGCR or SQLE with siRNA.Finally,the pull-down and surface plasmon resonance technology revealed that PPI could directly bind with SQLE but not with HMGCR.Collectively,these data demonstrated that PPI-induced hepatotoxicity resulted from the direct binding with SQLE protein and impaired the sterol-regulatory element binding protein 2/HMGCR/SQLE/lanosterol synthase pathways,thus disturbing the cholesterol biosynthesis pathway.The findings of this research can contribute to a better understanding of the key role of SQLE as a potential target in drug-induced hepatotoxicity and provide a therapeutic strategy for the prevention of drug toxic effects with similar structures in the future. | Zhiqi Li Qiqi Fan Meilin Chen Ying Dong Farong Li Mingshuang Wang Yulin Gu Simin Guo Xianwen Ye Jiarui Wu Shengyun Dai Ruichao Lin Chongjun Zhao | 2023 | Journal of Pharmaceutical Analysis2023，13，1： | 2 |
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