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| 1 | Biodegradable Materials for Bone Repairs:A Review显示文摘With attractive research and development of biomaterials,more and more opportunities have been brought to the treatments of human tissue repairs.The implant is usually no need to exist in the body accompanied with the recovery or regeneration of the tissue lesions,and the long-term effect of exotic substance to human body should be reduced as lower as possible.For this purpose,biodegradable materials,including polymers, magnesium alloys and ceramics,have attracted much attention for medical applications due to their biodegradable characters in body environment.This paper in turn introduces these three different types of widely studied biodegradable materials as well as their advantages as implants in applications for bone repairs.Relevant history and research progresses are summarized. | Lili Tan Xiaoming Yu Peng Wan Ke Yang | 2013 | Journal of Materials Science & Technology2013,29,6: | 24 |
| 2 | Tumor suppressor miR-128-3p inhibits metastasis and epithelial-mesenchymal transition by targeting ZEB1 in esophageal squamous-cell cancer显示文摘MicroRNAs (miRNAs ) 是在 post-transcriptional 层次调整多重生物功能的一些短 RNA,例如 tumorigenic 过程,煽动性的损害和房间 apoptosis。(ZEB1 ) 锌手指电子盒子有约束力的 homeobox 因素 1 是上皮间充质的转变(EMT ) 的一个关键调停人。它包括人的食道的有鳞房间的癌(ESCC ) 导致各种各样的癌症的恶意的前进。在这研究,我们发现 miR-128-3p 由使用 PCR 是在 ESCC 纸巾和房间的 downregulated。而且, miR-128-3p 的下面调整的表示被证明与 ESCC 病人的差的预后被联系并且可能被认为是一个独立预示的因素。然后,我们在 ESCC 房间检验了 miR-128-3p 的角色,并且发现 miR-128-3p 能在 vitro 压制房间迁居和侵略。而且, ZEB1 被证实是由酶记者试金的 miR-128-3p 的一个直接目标。营救实验证明 EMT 被 miR-128-3p 经由 ZEB1 的抑制调整。一起拿所有,我们断定 miR-128-3p 压制 EMT,经由 ZEB1 的转移,和 miR-128-3p 可以是在 ESCC 的一个批评调停人。 | Lili Zhao Rui Li Shanling Xu Yi Li Pei Zhao Wei Dong Zhenjun Liu Qian Zhao Bo Tan | 2018 | Acta Biochimica et Biophysica Sinica2018,50,2: | 21 |
| 3 | Surface Modification on Biodegradable Magnesium Alloys as Orthopedic Implant Materials to Improve the Bio-adaptability:A Review显示文摘Magnesium(Mg) and its alloys as a novel kind of biodegradable material have attracted much fundamental research and valuable exploration to develop its clinical application. Mg alloys degrade too fast at the early stage after implantation, thus commonly leading to some problems such as osteolysis, early fast mechanical loss, hydric bubble aggregation, gap formation between the implants and the tissue. Surface modification is one of the effective methods to control the degradation property of Mg alloys to adapt to the need of organism. Some coatings with bioactive elements have been developed, especially for the micro-arc oxidation coating, which has high adhesion strength and can be added with Ca, P, and Sr elements. Chemical deposition coating including bio-mimetic deposition coating, electro-deposition coating and chemical conversion coating can provide good anticorrosion property as well as better bioactivity with higher Ca and P content in the coating. From the biodegradation study, it can be seen that surface coating protected the Mg alloys at the early stage providing the Mg alloy substrate with lower degradation rate. The biocompatibility study showed that the surface modification could provide the cell and tissue stable and weak alkaline surface micro-environment adapting to the cell adhesion and tissue growth.The surface modification also decreased the mechanical loss at the early stage adapting to the loadbearing requirement at this stage. From the interface strength between Mg alloys implants and the surrounding tissue study, it can be seen that the surface modification improved the bio-adhesion of Mg alloys with the surrounding tissue, which is believed to be contributed to the tissue adaptability of the surface modification. Therefore, the surface modification adapts the biodegradable magnesium alloys to the need of biodegradation, biocompatibility and mechanical loss property. For the different clinical application, different surface modification methods can be provided to adapt to the clinical requirements for the Mg alloy implants. | Peng Wan Lili Tan Ke Yang | 2016 | Journal of Materials Science & Technology2016,32,9: | 19 |
| 4 | Biofunctional magnesium coated Ti6Al4V scaffold enhances osteogenesis and angiogenesis in vitro and in vivo for orthopedic application显示文摘The insufficient osteogenesis and osseointegration of porous titanium based scaffold limit its further application.Early angiogenesis is important for scaffold survival.It is necessary to develop a multifunctional surface on titanium scaffold with both osteogenic and angiogenic properties.In this study,a biofunctional magnesium coating is deposited on porous Ti6Al4V scaffold.For osseointegration and osteogenesis analysis,in vitro studies reveal that magnesium-coated Ti6Al4V co-culture with MC3T3-E1 cells can improve cell proliferation,adhesion,extracellular matrix(ECM)mineralization and ALP activity compared with bare Ti6Al4V cocultivation.Additionally,MC3T3-E1 cells cultured with magnesium-coated Ti6Al4V show significantly higher osteogenesisrelated genes expression.In vivo studies including fluorochrome labeling,micro-computerized tomography and histological examination of magnesium-coated Ti6Al4V scaffold reveal that new bone regeneration is significantly increased in rabbits after implantation.For angiogenesis studies,magnesium-coated Ti6Al4V improve HUVECs proliferation,adhesion,tube formation,wound-healing and Transwell abilities.HUVECs cultured with magnesium-coated Ti6Al4V display significantly higher angiogenesis-related genes(HIF-1αand VEGF)expression.Microangiography analysis reveal that magnesium-coated Ti6Al4V scaffold can significantly enhance the blood vessel formation.This study enlarges the application scope of magnesium and provides an optional choice to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and angiogenesis for further orthopedic applications. | Peng Gao Bo Fan Xiaoming Yu Wenwen Liu Jie Wu Lei Shi Di Yang Lili Tan Peng Wan Yulin Hao Shujun Li Wentao Hou Ke Yang Xiaokang Li Zheng Guo | 2020 | Bioactive Materials2020,5,3: | 15 |
| 5 | Study on β-TCP Coated Porous Mg as a Bone Tissue Engineering Scaffold Material显示文摘Three-dimensional honeycomb-structured magnesium (Mg) scaffolds with interconnected pores of accurately controlled pore size and porosity were fabricated by laser perforation technique. Biodegradable and bioactive β-tricalcium phosphate (β-TCP) coatings were prepared on the porous Mg to further improve its biocompatibility, and the biodegradation mechanism was simply evaluated in vitro. It was found that the mechanical properties of this type of porous Mg significantly depended on its porosity. Elastic modulus and compressive strength similar to human bones could be obtained for the porous Mg with porosity of 42.6%-51%. It was observed that the human osteosarcoma cells (UMR106) were well adhered and proliferated on the surface of the β-TCP coated porous Mg, which indicates that the β-TCP coated porous Mg is promising to be a bone tissue engineering scaffold material. | Fang Geng Lili Tan Bingchun Zhang Chunfu Wu Yonglian He Jingyu Yang Ke Yang | 2009 | Journal of Materials Science & Technology2009,25,1: | 13 |
| 6 | Effect of minor content of Gd on the mechanical and degradable properties of as-cast Mg-2Zn-xGd-0.5Zr alloys显示文摘RE-containing Mg alloys used as biodegradable medical implants exhibit good promising application due to their good mechanical properties and degradation resistance. In this work, effect of Gd on the microstructure, mechanical properties and biodegradation of as-cast Mg-2Zn-xGd-0.5Zr alloys was investigated. The results showed that there were mainly α-Mg, I-phase, W-phase and MgZn2 phase in Mg-Zn-Gd-Zr alloys. With increase of the Gd content, the strength of the alloys was enhanced due to the second phase strengthening and grain refinement. The degradation resistance of Mg-2Zn-0.5Zr alloy was increased by adding 0.5%–1% Gd due to the uniformly distributed second phases which acted as a barrier to prevent the pitting corrosion. However, increasing Gd content to 2% reduced the degradation resistance of the alloy due to the galvanic corrosion between the matrix and the second phases.The good degradation resistance and mechanical properties of as-cast Mg-2Zn-1Gd-0.5Zr alloy makes it outstanding for biomaterial application. | Junxiu Chen Lili Tan Xiaoming Yu Ke Yang | 2019 | Journal of Materials Science & Technology2019,35,4: | 13 |
| 7 | Mechanical properties, biodegradability and cytocompatibility of biodegradable Mg-Zn-Zr-Nd/Y alloys显示文摘Biodegradable magnesium alloys have been turned out to be a promising candidate for orthopedic applications.In this study,the mechanical properties,degradation behaviorand cytocompatibility of MgZn-Zr-Nd and Mg-Zn-Zr-Y alloys were studied in comparison with pure Mg.Mechanical tests showed that the strength and ductility of Mg-Zn-Zr-Nd and Mg-Zn-Zr-Y alloys were excellent.The corrosion resistance analyzed by electrochemical test and immersion test in alpha modified eagle(α-MEM) medium with 10% fetal bovine serum(FBS) revealed the degradation of Mg-Zn-Zr-Nd and Mg-Zn-Zr-Y alloys were faster than pure Mg at an early stage but slowed down after long time immersion.The metal ion concentrations were consistent with the corrosion rate.Mg-Zn-Zr-Nd alloy shows better mechanical properties than pure Mg and better corrosion resistance than Mg-Zn-Zr-Y alloy.The direct and indirect in vitro tests with MC3 T3-E1 cells demonstrate that Mg-Zn-Zr-Nd shows the best cytocompatibility and osteogenesis.The results suggest that Mg-Zn-Zr-Nd alloy shows an ideal combination of mechanical,corrosive and biological properties.In summary,these results implying the Mg-Zn-Zr-Nd alloy has great potential to benefit the future development of orthopedic applications. | Shi Jin Dan Zhang Xiaopeng Lu Yang Zhang Lili Tan Ying Liu Qiang Wang | 2020 | Journal of Materials Science & Technology2020,47,12: | 12 |
| 8 | Fluoride Conversion Coating on Biodegradable AZ31B Magnesium Alloy显示文摘A fluoride conversion coating was successfully prepared on AZ31 B magnesium alloy by chemical reaction in hydrofluoric acid.Morphologies,composition,bonding strength,corrosion properties,in vitro cytotoxicity and antibacterial properties of the coating were investigated,respectively.The scanning electron microscopy observations revealed a dense coating with some irregular pores.The thin-film X-ray diffraction analysis indicated that the coating was mainly composed of MgO and MgF2.The electrochemical impedance spectroscopy results showed that the fluoride conversion coating significantly improved the corrosion resistance of AZ31 B.The hydroxyapatite formed on the surface of the fluoride coated AZ31 B after being immersed in the simulated blood plasma indicated the good bioactivity of the material.The in vitro cytotoxicity test showed that the fluoride coated AZ31 B alloy was not toxic to BMMSCs(human bone marrow-derived mesenchymal stem cells).It was also found that the fluoride coated AZ31 B alloy had antibacterial capability. | Tingting Yan Lili Tan Bingchun Zhang Ke Yang | 2014 | Journal of Materials Science & Technology2014,30,7: | 11 |
| 9 | Degradation and biological properties of Ca-P contained micro-arc oxidation self-sealing coating on pure magnesium for bone fixation显示文摘Poor corrosion resistance is one of the main disadvantages for biodegradable magnesium-based metals,especially applied for bone fixation,where there is a high demand of bio-mechanical strength and stability.Surface coating has been proved as an effective method to control the in vivo degradation.In this study a Ca-P self-sealing micro-arc oxidation(MAO)coating was studied to verify its efficacy and biological properties by in vitro and in vivo tests.It was found that the MAO coating could effectively retard the degradation according to immersion and electrochemical tests as well as 3D reconstruction by X-ray tomography after implantation.The MAO coating exhibited no toxicity and could stimulate the new bone formation.Therefore,the Ca-P self-sealing MAO coating could be a potential candidate for application of biodegradable Mg-based implant in bone fixations. | Weidan Wang Peng Wan Chen Liu Lili Tan Weirong Li Lugee Li Ke Yang | 2015 | Regenerative Biomaterials2015,2,2: | 11 |
| 10 | Suitable internal control genes for qRT-PCR normalization in cotton fiber development and somatic embryogenesis显示文摘The mechanisms of cotton fiber development and somatic embryogenesis have been explored sys-tematically with microarray and suppression subtractive hybridization. Real-time RT-PCR provides the simultaneous measurement of gene expression in many different samples,with which the data from microarray or others can be confirmed in detail. To achieve accurate and reliable gene expression re-sults,normalization of real-time PCR data against one or several internal control genes is required,which should not fluctuate in different tissues during various stages of development. We assessed the gene expression of 7 frequently used housekeeping genes,including 18S rRNA,Histone3,UBQ7,Actin,Cyclophilin,Gbpolyubiquitin-1 and Gbpolyubiquitin-2,in a diverse set of 21 cotton samples. For fiber developmental series the expression of all housekeeping genes had the same down tendency after 17 DPA. But the expression of the AGP gene(arabinogalactan protein) that has high expression level at the later fiber development stage was up-regulated from 15 to 27 DPA. So the relative absolute quanti-fication should be an efficient and convenient method for the fiber developmental series. The expres-sion of nonfiber tissues series varied not so much against the fiber developmental series. And three best control genes Histone3,UBQ7 and Gbpolyubiquitin-1 have to be used in a combinated way to get better normalization. | TU LiLi ZHANG XianLong LIU DiQiu JIN ShuangXia CAO JingLin ZHU LongFu DENG FengLin TAN JiaFu ZHANG CunBin | 2007 | Chinese Science Bulletin2007,52,22: | 10 |
| 11 | Disturbance of hippocampal H2S generation contributes to CUMS-induced depression-like behavior: involvement in endoplasmic reticulum stress of hippocampus显示文摘长期的无法预言的温和压力淫液模型是消沉的一个广泛地使用的试验性的模型。在马头鱼尾的怪兽的外长的导致压力的 neuronal 房间死亡仔细与消沉的致病被联系。过多、延长的 endoplasmic 蜂窝胃嗯压力扳机房间死亡。氢硫化物 H 2 S,第三内长的发信号 gasotransmitter,作为 neuromodulator 和 neuroprotectant 在大脑功能起一个重要作用。我们假设了内长的 H 2 S 产生并且嗯在马头鱼尾的怪兽的应力可能涉及导致淫液的像消沉的行为。因此,集中于是否达到高潮的现在的学习扰乱内长的 H 2 S 和起来调整嗯在马头鱼尾的怪兽的应力并且是否外长的 H 2 S 阻止导致淫液的象压抑一样行为。结果证明对待淫液的老鼠展出像消沉的行为并且海马趾嗯包括调整葡萄糖的蛋白质的起来调整的层次的压力回答 78, CCAAT/enhancer 有约束力的蛋白质相应蛋白质,和劈开的 caspase-12 表示,当时 H 2 在马头鱼尾的怪兽的 S 在对待淫液的老鼠被压制。而且,外长的 H 2 S 阻止导致淫液的像消沉的行为。这些数据显示导致淫液的像消沉的行为与内长的 H 2 S 产生并且嗯在马头鱼尾的怪兽的应力并且建议了那内长的 H 2 S 并且嗯应力是消沉的新奇处理目标。 | Huiying Tan Wei Zou Jiamei Jiang Ying Tian Zhifang Xiao Lili Bi Haiying Zeng Xiaoqing Tang | 2015 | Acta Biochimica et Biophysica Sinica2015,47,4: | 8 |
| 12 | Spatial distribution of heavy metals(Cu, Pb, Zn, and Cd)in sediments of a coastal wetlands in eastern Fujian, China显示文摘We investigated the spatial distribution(horizontal and vertical concentrations) of copper(Cu),lead(Pb), zinc(Zn), and cadmium(Cd) in five wetland types(mudflat, aquaculture wetland, water area, farmland wetland and mangrove) from three areas(Ningde, Fuding,and Xiapu), China. Cu concentrations in five wetland types descended in the order: farm wetland, mudflat, aquaculture,water area and mangrove. Pb concentrations decreased in the order: aquaculture, mangrove, farm wetland, mudflat,and water area. Zn content decreased in the order: farm wetland, water area, aquaculture, mudflat and mangrove,and Cd content decreased as follows: mangrove, aquaculture, water area, mudflat, and farm wetland. Comparison of the concentrations of the same heavy metals in different areas showed that the highest Cu(63.75 mg kg^-1) and Zn(152.32 mg kg^-1) concentrations occurred in Ningde coastal wetlands; Pb(110.58 mg kg^-1) and Cd(2.81 mg kg^-1) contents were highest in Fuding wetlands,and the average contents of all heavy metals were very low in Xiapu wetlands. Examination of the vertical distribution showed that the Cu content was high in all mudflat layers;Pb and Cd concentrations were highest in aquaculture and mangrove wetlands, respectively, and Zn content was highest in farm wetlands. The spatial distribution of Cu and Zn contents for different areas decreased as follows:Ningde [ Fuding [ Xiapu, for Pb and Cd were most concentrated in Fuding coastal wetlands. Concentrations of Zn and Cu were highly correlated, while Zn and Cu were not significantly correlated with Pb. | Lili Zhao Weibin You Haiqing Hu Wei Hong Xiaojuan LIAO Shihong Xiao Ren Wang Jinbiao Cai Xuncheng Fan Yong Tan Dongjin He | 2015 | Journal of Forestry Research2015,26,3: | 8 |
| 13 | Structural basis of AMPK regulation by adenine nucleotides and glycogen显示文摘激活安培的蛋白质 kinase (AMPK ) 为糖尿病,肥胖,和癌症的治疗是一个中央细胞的精力传感器和精力动态平衡,和一个有希望的药目标的管理者。这里我们人的 α 的现在的低分辨率的水晶结构; 1 β 2 γ 1 holo-AMPK 建筑群跳了到它的 allosteric 调节的人安培和肝糖模仿 cyclodextrin,两个在 phosphorylated (4.05 Å) 并且 non-phosphorylated (4.60 Å) 状态。另外,我们解决了 2.95 Å人的 kinase 领域(KD ) 的结构跳了到邻近的 autoinhibitory 领域(帮助) 并且表现了广泛生物化学并且 mutational 研究。一起,这些研究由安培和肝糖说明 allosteric AMPK 调整的内在的机制,其绑定改变在交替的帮助(安培) 和糖类绑定模块(肝糖) 之间的 equilibria 相互作用。 | Xiaodan Li Lili Wang X Edward Zhou Jiyuan Ke Parker W de Waal Xin Gu M H Eileen Tan Dongye Wang Donghai Wu H Eric Xu Karsten Melcher | 2015 | Cell Research2015,25,1: | 8 |
| 14 | Fabrication and Evaluation of a Bioactive Sr–Ca–P Contained Micro-Arc Oxidation Coating on Magnesium Strontium Alloy for Bone Repair Application显示文摘Considering the compatibility between degradation and bioactivity of magnesium-based implants for bone repair, micro-arc oxidation is used to modify the magnesium alloy surface in aqueous electrolytes,allowing strontium, calcium, and phosphorus to be incorporated into the coating. The thickness, composition, morphology and phase of this Sr–Ca–P containing coating are characterized by scanning electron microscopy equipped with energy dispersive X-ray spectrometer and X-ray diffraction. The in vitro and in vivo degradation of the coating is evaluated by immersion test, electrochemical test and implantation test. Moreover, the cytocompatibility is tested with osteoblast cell according to ISO 10993. The results show that Sr, Ca and P elements are incorporated into the oxide coating, and a refined structure with tiny discharging micro-pores is observed on the surface of the coating. The Sr–Ca–P coating possesses a better corrosion resistance in vitro and retards the degradation in vivo. Such coating is expected to have significant medical applications on orthopedic implants and bone repair materials. | Junjie Han Peng Wan Yu Sun Zongyuan Liu Xinmin Fan Lili Tan Ke Yang | 2016 | Journal of Materials Science & Technology2016,32,3: | 8 |
| 15 | Bph30confers resistance to brown planthopperby fortifying sclerenchyma in rice leaf sheaths显示文摘Phloem-feeding insects cause massive losses in agriculture and horticulture.Host plant resistance to phloem-feeding insects is often mediated by changes in phloem composition,which deter insect settling and feeding and decrease viability.Here,we report that rice plant resistance to the phloem-feeding brown planthopper(BPH)is associated with fortification of the sclerenchyma tissue,which is located just beneath the epidermis and a cell layer or two away from the vascular bundle in the rice leaf sheath.We found that BPHs prefer to feed on the smooth and soft region on the surface of rice leaf sheaths called the long-cell block.We identified Bph30 as a rice BPH resistance gene that prevents BPH stylets from reaching the phloem due to the fortified sclerenchyma.Bph30 is strongly expressed in sclerenchyma cells and enhances cellulose and hemicellulose synthesis,making the cell walls stiffer and sclerenchyma thicker.The structurally fortified sclerenchyma is a formidable barrier preventing BPH stylets from penetrating the leaf sheath tissues and arriving at the phloem to feed.Bph30 belongs to a novel gene family,encoding a protein with two leucine-rich domains.Another member of the family,Bph40,also conferred resistance to BPH.Collectively,the fortified sclerenchyma-mediated resistance mechanism revealed in this study expands our understanding of plant-insect interactions and opens a new path for controlling planthoppers in rice. | Shaojie Shi Huiying Wang Lingyun Nie Di Tan Cong Zhou Qian Zhang YiLi Bo Du Jianping Guo Jin Huang DiWu Xiaohong Zheng Wei Guan Junhan Shan Lili Zhu Rongzhi Chen Longjian Xue Linda L.Walling Guangcun He | 2021 | Molecular Plant2021,14,10: | 7 |
| 16 | Switching off IMMP2L signaling drives senescence via simultaneous metabolic alteration and blockage of cell death显示文摘 | Lifeng Yuan Linhui Zhai Lili Qian De Huang Yi Ding Handan Xiang Xiaojing Liu J. Will Thompson Juan Liu Yong-Han He Xiao-Qiong Chen Jing Hu Qing-Peng Kong Minjia Tan Xiao-Fan Wang | 2018 | Cell Research2018,28,6: | 7 |
| 17 | Biofunctional Mg coating on PEEK for improving bioactivity显示文摘High purity Mg was successfully coated on polyetheretherketone(PEEK)by vapor deposition method in order to improve the bioactivity including antibacterial property of PEEK implant.The morphology and elemental composition of the coating were characterized by scanning electron microscopy(SEM)and energy-dispersive spectroscopy(EDS),showing that the coating was mainly composed of Mg at deposition temperature of 175C,185C,200C and 230C.The higher the substrate temperature was,the larger the Mg particle size was.The coating degraded and gradually peeled off from the surface of PEEK after up to 21 days'immersion.It was found that the degradation of Mg coating could strongly kill Staphylococcus aureus with antibacterial rate reaching to 99%.Mg can be expected to be coated on those bio-inert biomaterials to offer specific bioactivities. | Xiaoming Yu Muhammad Ibrahim Zongyuan Liu Huazhe Yang Lili Tan Ke Yang | 2018 | Bioactive Materials2018,3,2: | 6 |
| 18 | Time series prediction of mining subsidence based on a SVM显示文摘In order to study dynamic laws of surface movements over coal mines due to mining activities,a dynamic prediction model of surface movements was established,based on the theory of support vector machines(SVM) and times-series analysis.An engineering application was used to verify the correctness of the model.Measurements from observation stations were analyzed and processed to obtain equal-time interval surface movement data and subjected to tests of stationary,zero means and normality.Then the data were used to train the SVM model.A time series model was established to predict mining subsidence by rational choices of embedding dimensions and SVM parameters.MAPE and WIA were used as indicators to evaluate the accuracy of the model and for generalization performance.In the end,the model was used to predict future surface movements.Data from observation stations in Huaibei coal mining area were used as an example.The results show that the maximum absolute error of subsidence is 9 mm,the maximum relative error 1.5%,the maximum absolute error of displacement 7 mm and the maximum relative error 1.8%.The accuracy and reliability of the model meet the requirements of on-site engineering.The results of the study provide a new approach to investigate the dynamics of surface movements. | Li Peixian Tan Zhixiang Yan Lili Deng Kazhong | 2011 | Mining Science and Technology2011,21,4: | 6 |
| 19 | Development and utilization of a new chemically-induced soybean library with a high mutation density显示文摘Mutagenized populations have provided important materials for introducing variation and identifying gene function in plants. In this study, an ethyl methanesulfonate(EMS)-induced soybean(Glycine max) population,consisting of 21,600 independent M_2 lines, was developed.Over 1,000 M_(4(5))families, with diverse abnormal phenotypes for seed composition, seed shape, plant morphology and maturity that are stably expressed across different environments and generations were identified. Phenotypic analysis of the population led to the identification of a yellow pigmentation mutant, gyl, that displayed significantly decreased chlorophyll(Chl) content and abnormal chloroplast development. Sequence analysis showed that gyl is allelic to Minn Gold, where a different single nucleotide polymorphism variation in the Mg-chelatase subunit gene(ChlI1a) results in golden yellow leaves. A cleaved amplified polymorphic sequence marker was developed and may be applied to marker-assisted selection for the golden yellow phenotype in soybean breeding. We show that the newly developed soybean EMS mutant population has potential for functional genomics research and genetic improvement in soybean. | Zhongfeng Li Lingxue Jiang Yansong Ma Zhongyan Wei Huilong Hong Zhangxiong Liu Jinhui Lei Ying Liu Rongxia Guan Yong Guo Longguo Jin Lijuan Zhang Yinghui Li Yulong Ren Wei He Ming Liu Nang Myint Phyu Sin Htwe Lin Liu Bingfu Guo Jian Song Bing Tan Guifeng Liu Maiquan Li Xianli Zhang Bo Liu Xuehui Shi Sining Han Sunan Hua Fulai Zhou Lili Yu Yanfei Li Shuang Wang Jun Wang Ruzhen Chang Lijuan Qiu | 2017 | Journal of Integrative Plant Biology2017,59,1: | 6 |
| 20 | Effect of grain refinement and crystallographic texture produced by friction stir processing on the biodegradation behavior of a Mg-Nd-Zn alloy显示文摘The application of a single pass of friction stir processing(FSP) to Mg-Nd-Zn alloy resulted in grain refinement, texture evolution and redistribution of second phases, which improved corrosion resistance.In this work, an as-rolled Mg-Nd-Zn alloy was subjected to FSP. The microstructure in the processed zone of the FS-400 rpm alloy exhibited refined grains, a more homogenous grain size distribution, less second phases, and stronger basal plane texture. The corrosion behavior assessed using immersion tests and electrochemical tests in Hank’s solution indicated that the FS-400 rpm alloy had a lower corrosion rate, which was attributed to the increase of basal plane intensity and grain refinement. The hardness was lowered slightly and the elongation was increased, which might be attributed to the redistribution of the crushed second phases. | Wen Zhang Lili Tan Dingrui Ni Junxiu Chen Ying-Chao Zhao Long Liu Cijun Shuai Ke Yang Andrej Atrens Ming-Chun Zhao | 2019 | Journal of Materials Science & Technology2019,35,5: | 6 |