|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | A Novel Coronavirus Genome Identified in a Cluster of Pneumonia Cases--Wuhan,China 2019−2020显示文摘Emerging and re-emerging pathogens are great challenges to the public health(1).A cluster of pneumonia cases with an unknown cause occurred in Wuhan starting on December 21,2019.As of January 20,2020,a total of 201 cases of pneumonia in China have been confirmed.A team of professionals from the National Health Commission and China CDC conducted epidemiological and etiological investigations.On January 3,2020,the first complete genome of the novelβgenus coronaviruses(2019-nCoVs)was identified in samples of bronchoalveolar lavage fluid(BALF)from a patient from Wuhan by scientists of the National Institute of Viral Disease Control and Prevention(IVDC)through a combination of Sanger sequencing,Illumina sequencing,and nanopore sequencing.Three distinct strains have been identified,the virus has been designated as 2019-nCoV,and the disease has been subsequently named novel coronavirus-infected pneumonia(NCIP). | Wenjie Tan Xiang Zhao Xuejun Ma Wenling Wang Peihua Niu Wenbo Xu George F.Gao Guizhen Wu | 2020 | China CDC weekly2020，2，4： | 95 |
| 2 | The First Imported Case of Monkeypox in the Mainland of China—Chongqing Municipality,China,September 16,2022显示文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3). | Hua Zhao Wenling Wang Li Zhao Sheng Ye Jingdong Song Roujian Lu Hua Zong Changcheng Wu Wei Huang Baoying Huang Yao Deng Ruhan A Wujuan Xie Li Qi Wenbo Xu Hua Ling Wenjie Tan | 2022 | China CDC weekly2022，4，38： | 35 |
| 3 | Corrigendum to “Properties improvement of SPEEK based proton exchange membranes by doping of ionic liquids and Y_2O_3”[Prog. Nat. Sci.: Mater. Int. 22 (1) (2012) 26-30] | Jianbin Chen a, Qiang Guo a, Dan Li a, Juying Tong a,b, Xia Li a a School of Materials Science and Engineering, Shanghai University, Shanghai 201800, China b School of Wenling, Zhejiang TV University, Wenling 317500, China | 2012 | Progress in Natural Science:Materials International2012，22，3： | 33 |
| 4 | Long non-coding RNAs in cancer显示文摘Long non-coding RNAs (lncRNAs) are a group of RNA transcripts that exceed 200 nt in length, yet lack significant open reading frames (ORFs) [1–4]. In contrast | GONG ZhaoJian ZHANG ShanShan ZHANG WenLing HUANG HongBin LI Qiao DENG Hao MA Jian ZHOU Ming XIANG Juan Juan WU MingHua LI XiaYu XIONG Wei LI XiaoLing LI Yong ZENG ZhaoYang LI GuiYuan | 2012 | Science China(Life Sciences)2012，55，12： | 22 |
| 5 | Age-related rhesus macaque models of COVID-19显示文摘Background:Since December 2019,an outbreak of the Corona Virus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus(SARS-CoV-2)in Wuhan,China,has become a public health emergency of international concern.The high fatality of aged cases caused by SARS-CoV-2 was a need to explore the possible age-related phenomena with non-human primate models.Methods:Three 3-5 years old and two 15 years old rhesus macaques were intratracheally infected with SARS-CoV-2,and then analyzed by clinical signs,viral replication,chest X-ray,histopathological changes and immune response.Results:Viral replication of nasopharyngeal swabs,anal swabs and lung in old monkeys was more active than that in young monkeys for 14 days after SARS-CoV-2 challenge.Monkeys developed typical interstitial pneumonia characterized by thickened alveolar septum accompanied with inflammation and edema,notably,old monkeys exhibited diffuse severe interstitial pneumonia.Viral antigens were detected mainly in alveolar epithelial cells and macrophages.Conclusion:SARS-CoV-2 caused more severe interstitial pneumonia in old monkeys than that in young monkeys.Rhesus macaque models infected with SARS-CoV-2 provided insight into the pathogenic mechanism and facilitated the development of vaccines and therapeutics against SARS-CoV-2 infection. | Pin Yu Feifei Qi Yanfeng Xu Fengdi Li Peipei Liu Jiayi Liu Linlin Bao Wei Deng Hong Gao Zhiguang Xiang Chong Xiao Qi Lv Shuran Gong Jiangning Liu Zhiqi Song Yajin Qu Jing Xue Qiang Wei Mingya Liu Guanpeng Wang Shunyi Wang Haisheng Yu Xing Liu Baoying Huang Wenling Wang Li Zhao Huijuan Wang Fei Ye Weimin Zhou Wei Zhen Jun Han Guizhen Wu Qi Jin Jianwei Wang Wenjie Tan Chuan Qin | 2020 | Animal Models and Experimental Medicine2020，3，1： | 22 |
| 6 | The mechanisms of sorafenib resistance in hepatocellular carcinoma:theoretical basis and therapeutic aspects显示文摘Sorafenib is a multikinase inhibitor capable of facilitating apoptosis,mitigating angiogenesis and suppressing tumor cell proliferation.In late-stage hepatocellular carcinoma(HCC),sorafenib is currently an effective first-line therapy.Unfortunately,the development of drug resistance to sorafenib is becoming increasingly common.This study aims to identify factors contributing to resistance and ways to mitigate resistance.Recent studies have shown that epigenetics,transport processes,regulated cell death,and the tumor microenvironment are involved in the development of sorafenib resistance in HCC and subsequent HCC progression.This study summarizes discoveries achieved recently in terms of the principles of sorafenib resistance and outlines approaches suitable for improving therapeutic outcomes for HCC patients. | Weiwei Tang Ziyi Chen Wenling Zhang Ye Cheng Betty Zhang Fan Wu Qian Wang Shouju Wang Dawei Rong F.P.Reiter E.N.De Toni Xuehao Wang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 19 |
| 7 | Differential Fault Attack on Camellia显示文摘 | ZHOU Yongbin WU Wenling XU Nannan FENG Dengguo | 2009 | Chinese Journal of Electronics2009，18，1： | 15 |
| 8 | Relative humidity reconstruction for northwestern China's Altay Mountains using tree-ring δ^(18)O显示文摘Relative humidity is an important factor in water and water vapor feedback cycles.In this study,we established a 222-year annual tree-ring δ^(18)O chronology for Siberian larch(Larix sibirica Ldb.)from the Altay Mountains in northwestern China.Climate response analyses revealed that the relative humidity was the primary factor limiting tree-ring δ^(18)O fractionation.Based on our analysis,tree-ring δ^(18)O can be used to reconstruct the July–August relative humidity based on both a reasonable mechanism of tree-ring δ^(18)O fractionation and a statistically significant regression model.We used this model to reconstruct variations in the July–August relative humidity,and the model explained 47.4% of the total variation in the measured relative humidity data from 1961 to 2011.The relative humidity in the study area increased from 1900 to the 1990s and decreased thereafter.Two regime-shift dry periods were detected during the study period(one from 1817 to 1830 and the other from 2004 to 2011). | Guobao Xu Xiaohong Liu Dahe Qin Tuo Chen Wenzhi Wang Guoju Wu Weizhen Sun Wenling An Xiaomin Zeng | 2014 | Chinese Science Bulletin2014，59，2： | 14 |
| 9 | Moisture variations over the past millennium characterized by Qaidam Basin tree-ring δ^(18)O显示文摘High-resolution tree-ring δ18O chronologies covering the last millennium,although scarce,are essential in understanding patterns of climatic changes in the northeastern region of the Qinghai-Tibetan Plateau.For this study,a tree-ring δ18O chronology with a temporal resolution of 3-years was developed from the long-lived Qilian juniper(Sabina przewalskii Kom.),extending back in time to AD 991.This long δ18O chronology was significantly correlated with the yearly δ18O in tree rings during the common period from 1800 to 2006,and was an effective proxy for relative humidity during the growing season.A low-frequency moisture pattern signified the occurrence of a slight drought during the Medieval Climate Anomaly,a marked occurrence of a wet period during the Little Ice Age,and a trend in increasing moisture levels,although lower than average,alongside the Twentieth Century warming trend.Comparisons to other hydroclimatic reconstructions indicate that this tree-ring18O chronology serves as a reliable paleo-humidity proxy for the Qaidam Basin as well as documenting details of past humidity levels in the region. | WANG WenZhi LIU XiaoHong XU GuoBao SHAO XueMei QIN DaHe SUN WeiZhen AN WenLing ZENG XiaoMin | 2013 | Chinese Science Bulletin2013，58，32： | 14 |
| 10 | Nasopharyngeal carcinoma:Advances in genomics and molecular genetics显示文摘Nasopharyngeal carcinoma (NPC) is a squamous-cell carcinoma that arises in the epithelial lining of the nasopharynx [1]. This neoplasm has a notable ethnic and geographic distribution,being of high prevalence in southern China but rare in other parts of the world [2]. | ZENG ZhaoYang HUANG HongBin ZHANG WenLing XIANC Bo ZHOU Ming ZHOU YanHong MA Jian YI Mei LI XiaYu LI XiaoLing XIONG Wei LI GuiYuan | 2011 | Science China(Life Sciences)2011，54，10： | 13 |
| 11 | 结核感染T细胞斑点试验在不同患者群中的检测结果分析显示文摘目的探讨影响结核感染T细胞斑点试验(T-SPOT.TB)检测结果的因素。方法收集2014年5月至2015年4月同济大学附属上海市肺科医院共1537例住院患者的临床资料。以《临床诊疗指南:结核病分册》的标准将患者分为临床诊断结核病组(1159例)和临床诊断非结核病组(378例);再经病原学诊断及随访排除不能做出最终诊断的39例患者后,将患者分为结核病组(1103例;包括经分枝杆菌培养及菌种鉴定确诊的229例和最终临床诊断的874例)和非结核病组(395例;包括既往有结核病史或肺部存在陈旧性病灶者94例,无病史者301例);1103例结核病组患者中,肺结核557例,结核性胸膜炎107例,淋巴结结核16例,骨关节结核51例,脑结核7例,多组织器官结核363例,其他肺外结核2例;395例非结核病组患者中,经分枝杆菌培养及菌种鉴定确诊的非结核分枝杆菌(NTM)感染患者93例。回顾性分析临床诊断结核病组和结核病组患者T-SPOT.TB检测结果的敏感度、特异度,结核病组与非结核病组患者T-SPOT.TB检测反应强度,不同结核病类型TSPOT.TB检测阳性率,以及NTM感染患者与结核病患者的年龄差异。结果(1)临床诊断结核病组T-SPOT.TB检测的敏感度为8L97%(950/1159)、特异度为53.44%(202/378);结核病组T-SPOT.TB检测的敏感度为83.77%(924/1103)、特异度为54.43%(215/395);结核病组中抗酸染色阳性患者T-SPOT.TB检测阳性率为90.73%(235/259);培养阳性患者T-SPOT.TB检测阳性率为92.58%(212/229)。(2)非结核病组有结核病史或肺部显示陈旧性结核病灶的患者与无病史者T-SPOT.TB检测的阳性率分别为69.15%(65/94)和38.21%(115/301),两组间差异有统计学意义(x^2=27.65,P=0.000)。(3)结核病组与非结核病组T-SPOT.TB检测结果以斑点计数表示反应强度,结核病组对A、B抗原刺激出现阳性反应(斑点数≥1)的患者中,明显阳性及超强阳性(斑点数≥11)的比率分别为69.81%(652/934)和69.67%(627/900),明显高于非结核病组[分别为46.83%(96/205)和46.63%(83/178)];经曼-惠特尼秩和检验,差异有统计学意义(Z值分别为-14.20、-14.63,P值均d0.01)。(4)1103例结核病组患者中,T-SPOT.TB检测阳性率以淋巴结结核(87.50%,14/16)为最高,随后依次为多组织器官结核(87.33%,317/363)、肺结核(86.54%,482/557)、结核性胸膜炎(74.77%,80/107),脑结核(57.14%,4/7)和骨关节结核(50.98%,26/51)相对较低。(5)T-SPOT.TB检测阳性的NTM感染患者的平均年龄[(53.61±18.43)岁]较T—SPOT.TB检测阳性的确诊结核病患者[(44.98±18.88)岁]高,差异有统计学意义(t=-2.63,P=0.009)。结论T-SPOT.TB检测结果受结核病诊断依据、痰菌量、既往结核病史或肺部陈旧性结核病灶、NTM感染、结核病灶存在的部位等多种因素的影响;T-SPOT.TB检测用于指导结核病诊断时,需综合考虑各项因素。 | 尹洪云 WenLing Tan 马俊 葛燕萍 史祥 肖和平 冯永红 | 2018 | 中国防痨杂志2018，40，4： | 13 |
| 12 | Identification of aberrant cell cycle regulation in Epstein-Barr virus-associated nasopharyngeal carcinoma by cDNA microarray and gene set enrichment analysis显示文摘 | Wenling Zhang Zhaoyang Zeng Yanhong Zhou Wei Xiong Songqing Fan Lan Xiao Donghai Huang Zheng Li Dan Li Minghua Wu Xiaoling Li Shourong Shen Rong Wang Li Cao Ke Tang Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，5： | 11 |
| 13 | A novel human mAb (MERS-GD27) provides prophylactic and postexposure efficacy in MERS-CoV susceptible mice显示文摘Dear Editor,Since September 2012, the Middle East respiratory syndrome coronavirus (MERS-CoV) cases have been reported in more than 27 countries, and more than 2,000 cases have been confirmed in the laboratory (http://gffzz36b398b81f8a4a4chbnowo59n09xn6cop.ffgz.tsg.suse.edu.cn/emergencies/mers-cov/en/). MERS-CoV causes an acute and severe respiratory illness with a high mortality rate(~35%) in humans (Shi et al., 2017, Zaki et al., 2012).Neutralizing antibodies targeting the spike of MERS-CoV have been shown to be a therapeutic option for treatment of lethal disease (Agrawal et al., 2016, Ying et al., 2014). | Peihua Niu Guangyu Zhao Yao Deng Shihui Sun Wenling Wang Yusen Zhou Wenjie Tan | 2018 | Science China(Life Sciences)2018，61，10： | 11 |
| 14 | Preparation of polyclonal antibody specific for NOR1 and detection of its expression pattern in human tissues and nasopharyngeal carcinoma显示文摘包含蛋白质 1 的 Oxidored-nitro 领域(NOR1 ) 基因是首先从鼻咽的癌(NPC ) 孤立的新奇 nitroreductase 基因。它为它的 nitrosation 功能在化学致癌物的形成和 NPC 的 carcinogenesis 起一个重要作用。在鼻咽的癌细胞的野类型的 NOR1 基因的 Overexpression 是有效的禁止细胞生长和增长。在这研究,第一次,我们产生了高度特定的 NOR1 抗体并且在人的纸巾和 NPC 活体检视分析了 NOR1 分发。结果证明那 NOR1 蛋白质主要在人的鼻咽和气管的纸巾被表示。人的心,肝,怒气,胃,冒号,肾,骨胳的肌肉,胸腺,和胰都 NOR1 蛋白质是缺乏的。更重要地,我们在 NPC 纸巾执行了 NOR1 蛋白质表示的 immunohistochemistry 试金,并且结果证明那 NOR1 蛋白质在 NPC 经常是下面表示的。这些数据使 NOR1 的潜在的生理的函数的选择清楚些并且提供不可缺少的引用给 NPC 的 carcinogenesis 进程并且到识别或验证织物特定的药目标。 | Bo Xiang Mei Yi Li Wang Wei Liu Wenling Zhang Jue Ouyang Ya Peng Wenjuan Li Ming Zhou Huaying Liu Minghua Wu Rong Wan Xiaoling Li Guiyuan Li | 2009 | Acta Biochimica et Biophysica Sinica2009，41，9： | 10 |
| 15 | Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas显示文摘Epstein-Barr virus(EBV)is associated with nasopharyngeal carcinoma(NPC)tumorigenesis.However,the mechanism(s)connecting EBV infection and NPC remain unclear.Recently,a new class of EBV microRNAs(miRNAs)has been described.To determine how EBV miRNAs control the expression of host genes,and to understand their potential role in NPC tumorigenesis,we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyngeal epithelial tissues.We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC.Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the carcinogenesis of NPC.A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated,suggesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis.An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis. | ZENG ZhaoYang HUANG HongBin HUANG LiLi SUN MengXi YAN QiJia SONG YaLi WEI Fang BO Hao GONG ZhaoJian ZENG Yong LI Qiao ZHANG WenLing LI XiaYu XIANG Bo LI XiaoLing LI Yong XIONG Wei LI GuiYuan | 2014 | Science China(Life Sciences)2014，57，3： | 10 |
| 16 | Generation of GGTA1 biallelic knockout pigs via zinc-finger nucleases and somatic cell nuclear transfer显示文摘Genetically modified pigs are valuable models of human disease and donors of xenotransplanted organs.Conventional gene targeting in pig somatic cells is extremely inefficient.Zinc-finger nuclease(ZFN)technology has been shown to be a powerful tool for efficiently inducing mutations in the genome.However,ZFN-mediated targeting in pigs has rarely been achieved.Here,we used ZFNs to knock out the porcineα-1,3-galactosyl-transferase(GGTA1)gene,which generates Gal epitopes that trigger hyperacute immune rejection in pig-to-human transplantation.Primary pig fibroblasts were transfected with ZFNs targeting the coding region of GGTA1.Eighteen mono-allelic and four biallelic knockout cell clones were obtained after drug selection with efficiencies of 23.4%and 5.2%,respectively.The biallelic cells were used to produce cloned pigs via somatic cell nuclear transfer(SCNT).Three GGTA1 null piglets were born,and one knockout primary fibroblast cell line was established from a cloned fetus.Gal epitopes on GGTA1 null pig cells were completely eliminated from the cell membrane.Functionally,GGTA1 knockout cells were protected from complement-mediated immune attacks when incubated with human serum.This study demonstrated that ZFN is an efficient tool in creating gene-modified pigs.GGTA1 null pigs and GGTA1 null fetal fibroblasts would benefit research and pig-to-human transplantation. | BAO Lei CHEN HaiDe JONG UiMyong RIM CholHo LI WenLing LIN XiJuan ZHANG Dan LUO Qiong CUI Chun HUANG HeFeng ZHANG Yan XIAO Lei FU ZhiXin | 2014 | Science China(Life Sciences)2014，57，2： | 9 |
| 17 | Water Cooperation Priorities in the Lancang-Mekong River Basin Based on Cooperative Events Since the Mekong River Commission Establishment显示文摘The Lancang-Mekong River has attracted much attention from researchers, but the cooperation on water issues in this river basin has been limited, even after the establishment of the Mekong River Commission(MRC). Cooperation on water resources has been determined as one of the key priority areas in the Lancang-Mekong Cooperation Mechanism, but there are no details of targets. In order to establish the priorities of water cooperation under the mechanism, we adopted nine categories to classify the objectives of 87 water cooperation events based on the ‘Lancang-Mekong Water Cooperative Events Database' from 1995 to 2015. Based on the occurrence of cooperative events, cooperative objectives, cooperative scales, and approaches to cooperation, we conducted statistical, correlation, and text analyses. Our analyses indicated the following results: under the impact of economic conditions inside and outside the river basin, full cooperation appeared more difficult than bilateral and multilateral cooperation. Each of the partners adopted different preferences for cooperation targets. Cooperation with more definite objectives was easier to establish than cooperation with broader and more complex objectives. The potential objectives for water cooperation were navigation, hydropower, joint management, data sharing, flood control and water use. Because hydropower development is controversial, and because water cooperation is avoided by most existing regional cooperation mechanisms due to its complexity, we suggest the following priority areas for water cooperation in the Lancang-Mekong River Basin. 1) Navigation and flood control/drought relief are attractive objectives for all the riparian countries across the whole watershed. 2) Data sharing should be a priority for cooperation in the watershed due to its laying the foundation for the equitable and reasonable utilization of transboundary waters. 3) Hydropower is an objective best implemented mainly through bilateral cooperation, and on tributaries. | FENG Yan WANG Wenling SUMAN Daniel YU Shiwei HE Daming | 2019 | Chinese Geographical Science2019，29，1： | 8 |
| 18 | Three Novel Real-Time RT-PCR Assays for Detection of COVID-19 Virus显示文摘What is already known on this topic?A novel human coronavirus,known as SARS-CoV-2 or 2019-nCoV,is the causative agent of the coronavirus disease 2019(COVID-19).We have released the primers and probes of real-time reverse transcription polymerase chain reaction(rRT-PCR)assays for the laboratory detection of COVID-19 infection.What is added by this report?Here we provide detailed technical data and evaluate the performance of three novel rRT-PCR assays targeting the ORF1ab,N,and E genes for detection of COVID-19 infection.The application of rRT-PCR assays among four types of specimens(alveolar lavage,sputum,throat swabs,and stool)from patients with COVID-19 indicated that the mean viral loads detected in sputum were higher than other specimens.What are the implications for public health practice?These rRT-PCR assays reported here could be used for laboratory diagnosis of COVID-19 infection with high sensitivity,specificity,and applicability.Sputum rather than throat swabs and stool should be a priority for specimen collection for laboratory detection of COVID-19. | Peihua Niu Roujian Lu Li Zhao Huijuan Wang Baoying Huang Fei Ye Wenling Wang Wenjie Tan | 2020 | China CDC weekly2020，2，25： | 8 |
| 19 | FAM19A4 is a novel cytokine ligand of formyl peptide receptor I (FPR 1) and is able to promote the migration and phagocytosis of macrophages显示文摘FAM19A4 是为有顺序类似 19 的家庭的缩写(chemokine (C-C 主题)-like) 成员 A4,它能分泌的蛋白质在正常纸巾在底层被表示。坚定的 FAM19A4 遗体的生物功能,和它的潜在的受体是 unclarified。在这研究,我们证明 FAM19A4 是古典能分泌的蛋白质,我们第一次证实它的成熟蛋白质由 95 氨基酸组成。我们发现这新奇 cytokine 的表示是 upregulated 在里面 lipopolysaccharide (LPS ) 刺激了单核白血球和巨噬细胞并且典型地在极化的 M1。FAM19A4 在巨噬细胞上显示出趋化性的活动并且随蛋白质 kinase B (Akt ) 的 phosphorylation 的显著增加在 vitro 并且在 vivo 提高 zymosan 的巨噬细胞吞噬作用。FAM19A4 能也在 zymosan 刺激之上增加反应的氧种类(ROS ) 的版本。基于受体成为主观,收音机 ligand 绑定试金和受体阻塞,而且,我们第一次证明 FAM19A4 是甲酰肽受体 1 的新奇 ligand (FPR1 ) 。上述数据显示在煽动性的刺激之上, monocyte/macrophage-derived FAM19A4 可以在病原的感染期间在巨噬细胞的移植和激活起一个关键作用。 | Wenyan Wang Ting Li Xiaolin Wang Wanxiong Yuan Yingying Cheng Heyu Zhang Enquan Xu Yingmei Zhang Shuang Shi Dalong Ma Wenling Han | 2015 | Cellular & Molecular Immunology2015，12，5： | 8 |
| 20 | Identification of HBsAg-specific antibodies from a mammalian cell displayed full-length human antibody library of healthy immunized donor显示文摘肝炎 B 免疫球蛋白(HBIG ) 在肝炎 B 的管理是重要的病毒(HBV ) 感染。试图作为 HBIG 的一种选择开发 recombinant monoclonal 抗体,我们报导 HBV 表面抗原(HBsAg ) 的成功的鉴定从在哺乳动物的房间上显示的一个全身的人的抗体图书馆的特定的抗体出现。把一个生来地使免疫的施主的外部血 mononuclear 房间的全部的 RNA 用作模板,抗体全部剧目被放大。联合四方法的结扎和 Flp 调停 recombinase 的集成(Flp 在里面) 系统,我们构造了每个房间在显示了一个种仅仅抗体分子的一个哺乳动物的基于房间的、充分人的、全身的抗体显示图书馆。由屏蔽使用激活荧光的房间排序的房间图书馆(FACS ) ,八个房间克隆显示那房间表面上的 HBsAg 特定的抗体被识别。抗体基因的 DNA 顺序分析揭示了三唯一的抗体。FACS 数据显示了那荧光灯表示(FSE ) 的力量,荧光灯绑定(FSB ) 的力量和相对有约束力的能力(RBA ) 都在他们之中是不同的。这些结果由使用我们的抗体表明了那哺乳动物的显示和屏蔽站台,我们能成功地从一个使免疫的全身的抗体图书馆识别抗原特定的抗体。因此,这个平台为治疗学的抗体的开发是很有用的。 | Chang-Zheng Li Zhong-Kun Liang Zhen-Rui Chen Hai-Bo Lou Ye Zhou Zhe-Huan Zhang Fei Yu Shuwen Liu Yuanping Zhou Shuguang Wu Wenling Zheng Wanlong Tan Shibo Jiang Chen Zhou | 2012 | Cellular & Molecular Immunology2012，9，2： | 7 |
您所在的机构暂未开通此部分的功能，请联系管理员