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| 1 | Hypoxia and cytokines regulate carbonic anhydrase 9 expression in hepatocellular carcinoma cells in vitro显示文摘AIM: To study the expression of carbonic anhydrase(CA) 9 in human hepatocellular carcinoma(HCC) cells.METHODS: We studied CA9 protein, CA9 m RNA and hypoxia-inducible factor-1 alpha(HIF-1α) protein levels in Hep3 B cells exposed in different parallel approaches. In one of these approaches, HCC cells were exposed to extreme in vitro hypoxia(24 h 0.1% O2) without or with interleukin(IL)-1, IL-6, tumor necrosis factoralpha(TNF-α) and transforming growth factor-beta(TGF-β) stimulation for the same hypoxic exposure time or exposed to normoxic oxygenation conditions without or with cytokine stimulation.RESULTS: The tumour cell line analysed showed a strong hypoxic CA9 m RNA expression pattern in response to prolonged severe hypoxia with cell-line specific patterns and a marked induction of CA9 protein in response to severe hypoxia. These results were paralleled by the results for HIF-1α protein under identical oxygenation conditions with a similar expression tendency to that displayed during the CA9 protein expression experimental series. Continuous stimulation with the cytokines, IL-1, IL-6, TNF-α and TGF-β, under normoxic conditions significantly increased the carbonic anhydrase 9 expression level at both the protein and m RNA level, almost doubling the CA9 m RNA and CA9 and HIF-1α protein expression levels found under hypoxia. The findings from these experiments indicated that hypoxia is a positive regulator of CA9 expression in HCC, and the four signal transduction pathways, IL-1, IL-6, TNF-α and TGF-β, positively influence CA9 expression under both normoxic and hypoxic conditions.CONCLUSION: These findings may potentially be considered in the design of anti- cancer therapeutic approaches involving hypoxia-induced or cytokine stimulatory effects on expression. In addition, they provideevidence of the stimulatory role of the examined cytokine families resulting in an increase in CA9 expression under different oxygenation conditions in human cancer, especially HCC, and on the role of the CA9 gene as a positive disease regulator in human cancer. | Feray Kockar Hatice Yildrim Rahsan Ilikci Sagkan Carsten Hagemann Yasemin Soysal Jelena Anacker Ahmed Ayad Hamza Dirk Vordermark Michael Flentje Harun M Said | 2012 | World Journal of Clinical Oncology2012，3，6： | 2 |
| 2 | Magnitude and clinical relevance of translational and rotational patient setup errors a cone-beam CT study 显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int Radiat Oncol Biol Phys2006，65，3： | 1 |
| 3 | Magnitude andclinical relevance of translational and rotational patient setup er-rors :a cone - l)eam CT study 显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，3： | 1 |
| 4 | Cell type-specific association of hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein accumulation and radiobiologic tumor hypoxia显示文摘 | Vordermark D Katzer A Baier K | 2004 | Int J Radiat Oncol Biol Phys2004，58，4： | 1 |
| 5 | Evaluation of hypoxia-inducible factorlalpha (HIF-lalpha) as an intrinsic marker of tumor hypoxia in U87 MG human glioblastoma: in vitro and xenograft studies显示文摘 | Vordermark D Brown JM | 2003 | Int J Radiat Oncol Biol Phys2003，56，4： | 1 |
| 6 | Cell type - specific association of hypoxia - inducible factor - 1 alpha ( HIF - 1 alpha ) protein accumulation and radiobiologic tumor hypoxia 显示文摘 | VORDERMARK D KATZER A BAIER K | 2004 | Int J Radiat Oncol Biol Phys2004，58，4： | 1 |
| 7 | Magnitude and clinical relevance of translational and rotational patient setup errors: A cone-beam CT study显示文摘 | Matthias Guckenberger Juergen Meyer Dirk Vordermark Kurt Baier Juergen Wilbert Michael Flentje | 2006 | International Journal of Radiation Oncology, Biology, Physics2006，，： | 1 |
| 8 | Job stress and job satis-faction of physicians, radiographers f nurses and physicists working inradiotherapy : a multicenter analysis by the DEGRO quality of life workgroup 显示文摘 | Sehlen S Vordermark D Schafer C | 2009 | Hadiat Oncol2009，4，1： | 1 |
| 9 | Magni- tude and clinical relevance of translational and rotational patient setup errors:A cone-beam CT study显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，3： | 1 |
| 10 | Magnitude and clinical relevance of translational and rotational patient setup errors : a cone -beam CT study显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | International Journal of Radiation On- cology Biology Physics2006，65，3： | 1 |
| 11 | Cell type - specific asso- ciation of hypoxia - inducible factor - 1 alpha ( HIF - 1 alpha) protein accumulation and radiobiologic tumor hypoxia 显示文摘 | Vordermark D Katzer A Baier K | 2004 | Int J Radiat Oncol Biol Phys2004，58，4： | 1 |
| 12 | Magnitude and clinical relevance of translational and rotational patient setup errors:a cone-beam CT study显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，3： | 1 |
| 13 | Magnitude and clinical relevance of translational and rotational patient setup errors: A cone-beam CT study 显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，3： | 1 |
| 14 | Cell type-specific association of hypoxia-inducible factor-1 alpha (HIF-1 alpha) protein accumulation and radiobiologic tumor hypoxia显示文摘 | Vordermark D Katzer A Baier K | 2004 | Int J Radiat Oncol Biol Phys2004，58，4： | 1 |
| 15 | Magnitude and clinical relevance of translational and rotational patient set-up errors显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，： | 1 |
| 16 | Cardiotoxicity and oncological treatments显示文摘 | SCHLITT A JORDAN K VORDERMARK D | 2014 | Dtsch Arztebllnt2014，，10： | 1 |
| 17 | Magnitude and clinical relevance of translational and rotational patient setup errors: a cone-beam CT study 显示文摘 | Guckenberger M Meyer J Vordermark D | 2006 | Int J Radiat Oncol Biol Phys2006，65，3： | 1 |
| 18 | The relationship between belly board position and patient anatomy and its influence on dose-volume histogram of small bowel for postoperative radiotherapy of rectal cancer 显示文摘 | Koelbl O Vordermark D Flentje M | 2003 | Radial Oncol2003，67，3： | 1 |
| 19 | Glioblastoma muhiforme with oligodendroglial component ( GBMO ) : favorable outcome after post-operative radiotherapy and chemotherapy with nimustine (ACNU) and teniposide ( VM26 ) 显示文摘 | Vordermark D Ruprecht K Rieckmann P | 2006 | BMC Cancer2006，6，： | 1 |
| 20 | Job stress and job satisfaction of physicians, radiographers, nurses and physicists working in radiotherapy: a muhieenter analysis by the DEGRO Quality of Life Work Group显示文摘 | SEHLEN S VORDERMARK D SCHAFER C | 2009 | Radiat Oncol2009，4，1： | 1 |
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