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| 1 | Antibodies Against Annexin V and Prothrombin,Their Correlation with Other Anti-phospholipid Antibodies in Recurrent Pregnancy Loss显示文摘Objective To study the findings of serum antibodies against annexin V, prothrombin,ph-inositol, ph-acid, ph-ethanolamine, ph-serine, ph-glycerol, cardiolipin, and beta2-glycoprotein I and analyze the trophoblast annexin V receptorsMethods Sera from 156 patients aged 26-41 years with recurrent pregnancy loss (3-7 times) were investigated. Eighty-four fertile healthy women aged 24-38 yearswere included in a control group. ELISA methods were used for detecting a panel of seraanti-phospholipid antibodies. Immunolocalization of annexin V receptors in 143trophoblast specimens of 156 patients was investigated by the immunofluorescencetechnique using Annexin V-FITC, Apoptosis and AnnexinV-CY3 commercial kits.Results Positivity for anti-phospholipid antibodies mainly against ph-serine, ph-ethanolamine, and ph-inositol was found together in 80.8% (126 out of 156 patients),anti-prothrombin antibodies in 12% (18), and anti-annexin V antibodies in 13. 5%(21) women. No significant levels of anti-phospholipid antibodies were found in 6controls. Placenta immunohistopathology also exhibited some changes manifested bythe presence of apoptotic and necrotic cells in trophoblast, and very fewmicrothrombotization in some intervillous spaces.Conclusion Our detailed study demonstrated the prevalence of majority of anti-phospholipid antibodies as a high risk factor for repeated reproductive failure. Verylow microthrombosis in placentas could be explained by the changes ofhaemocoagulation properties out of uterus. | Zdenka Ulcova-Gallova Petr Mukensnabl Sarka Hadravska Vendula Kyselova Jitka Slechtova Zdenka Micanova Katerina Bibkova Miroslav Balvin Zdenek Rokyta | 2005 | Journal of Reproduction and Contraception2005，16，1： | 3 |
| 2 | Effect of unburned carbon content in fly ash on the retention of 12 elements out of coal-combustion flue gas显示文摘The aim of this study was to evaluate whether unburned carbon particles present in fly ash can help in the retention of S,Cl,Br,As,Se,Cu,Ni,Zn,Ga,Ge,Rb,and Pb out of flue gas during the coal combustion at fluidised-bed power station where the coal was combusted along with limestone.The competitive influence of 10%-25% CaO in fly ashes on the distribution of studied elements was studied as well to be clear which factor governs behaviour of studied elements.Except of S(with significant association with CaO) and Rb and Pb(with major affinity to Al2O3) the statistically significant and positive correlation coefficients were calculated for the relations between unburned carbon content and Br(0.959),Cl(0.957),Cu(0.916),Se(0.898),Ni(0.866),As(0.861),Zn(0.742),Ge(0.717),and Ga(0.588) content.The results suggest that the unburned carbon is promising material in terms of flue gas cleaning even if contained in highly calcareous fly ashes. | Lucie Bartonov Bohumír ech Lucie Ruppenthalov Vendula Majvelderov Dagmar Juchelkov Zdeněk Klika | 2012 | Journal of Environmental Sciences2012，24，9： | 3 |
| 3 | Capillary elec- trophoresis determination of carnitine in food supple- ments显示文摘 | Vendula P FrantigeK Rudolfgevcik | 2005 | Chromatogr A2005，1081，： | 1 |
| 4 | Degradation of cellulose by basidiomycetous fungi 显示文摘 | Petr Baldfian Vendula Valtiskovti | 2008 | FEMS Microbiol Rev2008，32，3： | 1 |
| 5 | Body and organ weight,sperm acrosomal status and reproduction after genistein and diethylstilbestrol treatment of CD1 mice in a multigenerational study显示文摘 | Vendula K Jana P Michael B | 2004 | Theriogenology2004，61，： | 1 |
| 6 | Photocatalytic properties of Ru-doped titania prepared by homogeneous hydrolysis显示文摘 | Vendula Hou?ková Václav ?tengl Snejana Bakardjieva Nataliya Murafa Václav Tyrpekl | 2009 | Central European Journal of Chemistry2009，，2： | 1 |
| 7 | Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling显示文摘 | Lenka Doubravska Michaela Krausova Dietmar Gradl Martina Vojtechova Lucie Tumova Jan Lukas Tomas Valenta Vendula Pospichalova Bohumil Fafilek Jiri Plachy Ondrej Sebesta Vladimir Korinek | 2011 | Cellular Signalling2011，，5： | 1 |
| 8 | Visible-light photocatalytic activity of TiO 2 /ZnS nanocomposites prepared by homogeneous hydrolysis显示文摘 | Václav ?tengl Snejana Bakardjieva Nataliya Murafa Vendula Hou?ková Kamil Lang | 2007 | Microporous and Mesoporous Materials2007，，2： | 1 |
| 9 | Preparation, characterization and antimicrobial efficiency of Ag/PDDA-diatomite nanocomposite显示文摘 | Ale? Paná?ek Anna Balzerová Robert Prucek Václav Ranc Renata Ve?e?ová Vendula Husi?ková Ji?í Pechou?ek Jan Filip Radek Zbo?il Libor Kvítek | 2013 | Colloids and Surfaces B: Biointerfaces2013，，： | 1 |
| 10 | Spatial variability of enzyme activities and microbial biomass in the upper layers of Quercus petraea forest soil显示文摘 | Jaroslavnajdr Vendula Valáková Jana Herinková | 2008 | Soil Biology&Biochemistry2008，40，： | 1 |
| 11 | The development of free amino acids and volatile compounds in cheese Oloumoucke lvaruzky (PGI) during ripening显示文摘 | Vendula P Frantisek B Martina C | 2013 | International Journal of Food Science and Technology2013，48，9： | 1 |
| 12 | Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure显示文摘Background: Development of new nanomaterials that inhibit or kil bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles(NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens.Results: Four formulations of zinc nanoparticles(Zn A, Zn B, Zn C, and Zn D) based on phosphates with spherical(Zn A, Zn B)or irregular(Zn C, Zn D) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus(inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), fol owed by Escherichia coli(IC500.8–1.5 mmol/L). In contrast, methicil in resistant S. aureus(IC501.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial Zn O-based NPs and Zn O. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of Zn A, Zn B, Zn C, and Zn D, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of Zn O-N and Zn O, and one group(control) was fed only by basal diet. The significantly higher(P < 0.05) Zn level in liver and kidney of al treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However,the total aerobic and coliform bacterial population in rat feces significantly decreased(P < 0.05) in al zinc groups after 30 d of the treatment. Furthermore, when compared to the Zn O group, Zn A and Zn C nanoparticles reduced coliforms significantly more(P < 0.05).Conclusions: Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents. | Pavel Horky Sylvie Skalickova Lenka Urbankova Daria Baholet Silvia Kociova Zuzana Bytesnikova Eliska Kabourkova Zuzana Lackova Natalia Cernei Milica Gagic Vedran Milosavljevic Vendula Smolikova Eva Vaclavkova Pavel Nevrkla Pavel Knot Olga Krystofova David Hynek Pavel Kopel Jiri Skladanka Vojtech Adam Kristyna Smerkova | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 1 |
| 13 | Degradation of cellulose and hemi- celluloses by the brown rot fungus Piptoporus betulinus pro- duction of extracellular enzymes and characterization of the major cellulases 显示文摘 | VENDULA V PETER B | 2006 | Microbiology2006，152，12： | 1 |
| 14 | Fatty acid modification of Wnt1 and Wnt3a at serine is prerequisite for lipidation at cysteine and is essential for Wnt signalling显示文摘 | Lenka Doubravska Michaela Krausova Dietmar Gradl Martina Vojtechova Lucie Tumova Jan Lukas Tomas Valenta Vendula Pospichalova Bohumil Fafilek Jiri Plachy Ondrej Sebesta Vladimir Korinek | 2011 | Cellular Signalling2011，，5： | 1 |
| 15 | Structure and mechanical behaviour of powder processed dispersion strengthened copper 显示文摘 | SYNK J E VENDULA K | 1987 | Mater Sci Technol1987，3，： | 1 |
| 16 | Degradation of cellulose and hemicelluloses by the brown rot fungus Piptoporus betulinus: Production of extracellular enzymes and characterization of the major cellulases显示文摘 | Vendula V Peter B | 2006 | Microbiology2006，152，12： | 1 |
| 17 | Detection of NDM-1, VIM-l, KPC, OXA 48, and OXA162 carbapen creases by matrix-Assisted laser desorption ionization- time of flight mass spectrometry 显示文摘 | Jaroslav H Vendula B Radka W | 2012 | Clin Microhiol2012，50，7： | 1 |
| 18 | ETR1 Integrates Response to Ethylene and Cytokinins into a Single Multistep Phosphorelay Pathway to Control Root Growth显示文摘Cytokinins and ethylene control plant development via sensors from the histidine kinase(HK)family.However,downstream signaling pathways for the key phytohormones are distinct.Here we report that not only cytokinin but also ethylene is able to control root apical meristem(RAM)size through activation of the multistep phosphorelay(MSP)pathway.We found that both cytokinin and ethylene-dependent RAM shortening requires ethylene binding to ETR1 and the HK activity of ETR1.The receiver domain of ETR1 interacts with MSP signaling intermediates acting downstream of cytokinin receptors,further substantiating the role of ETR1 in MSP signaling.We revealed that both cytokinin and ethylene induce the MSP in similar and distinct cell types with ETR1-mediated ethylene signaling controlling MSP output specifically in the root transition zone.We identified members of the MSP pathway specific and common to both hormones and showed that ETR1-regulated ARR3 controls RAM size.ETR1-mediated MSP spatially differs from canonical CTR1/EIN2/EIN3 ethylene signaling and is independent of EIN2,indicating that both pathways can be spatially and functionally separated.Furthermore,we demonstrated that canonical ethylene signaling controls MSP responsiveness to cytokinin specifically in the root transition zone,presumably via regulation of ARR10,one of the positive regulators of MSP signaling in Arabidopsis. | Marketa Zdarska Abigail Rubiato Cuyacot Paul TTarr Amel Yamoune Agnieszka Szmitkowska Vendula Hrdinová Zuzana Gelová Elliot MMeyerowitz Jan Hejátko | 2019 | Molecular Plant2019，12，10： | 0 |
| 19 | FtsH4 protease controls biogenesis of the PSII complex by dual regulation of high light-inducible proteins显示文摘FtsH proteases are membrane-embedded proteolytic complexes important for protein quality control and regulation of various physiological processes in bacteria,mitochondria,and chloroplasts.Like most cyanobacteria,the model species Synechocystis sp.PCC 6803 contains four FtsH homologs,FtsH1–FtsH4.FtsH1–FtsH3 form two hetero-oligomeric complexes,FtsH1/3 and FtsH2/3,which play a pivotal role in acclimation to nutrient deficiency and photosystem Ⅱ quality control,respectively.FtsH4 differs from the other three homologs by the formation of a homo-oligomeric complex,and together with Arabidopsis thaliana AtFtsH7/9 orthologs,it has been assigned to another phylogenetic group of unknown function.Our results exclude the possibility that Synechocystis FtsH4 structurally or functionally substitutes for the missing or non-functional FtsH2 subunit in the FtsH2/3 complex.Instead,we demonstrate that FtsH4 is involved in the biogenesis of photosystem Ⅱ by dual regulation of high light-inducible proteins(Hlips).FtsH4 positively regulates expression of Hlips shortly after high light exposure but is also responsible for Hlip removal under conditions when their elevated levels are no longer needed.We provide experimental support for Hlips as proteolytic substrates of FtsH4.Fluorescent labeling of FtsH4 enabled us to assess its localization using advanced microscopic techniques.Results show that FtsH4 complexes are concentrated in well-defined membrane regions at the inner and outer periphery of the thylakoid system.Based on the identification of proteins that co-purified with the tagged FtsH4,we speculate that FtsH4 concentrates in special compartments in which the biogenesis of photosynthetic complexes takes place. | Vendula Krynická Petra Skotnicová Philip J.Jackson Samuel Barnett Jianfeng Yu Anna Wysocka Radek Kaña Mark J.Dickman Peter J.Nixon C.Neil Hunter Josef Komenda | 2023 | Plant Communications2023，4，1： | 0 |
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