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| 1 | Review of current diagnostic methods and advances in Helicobacter pylori diagnostics in the era of next generation sequencing显示文摘Helicobacter pylori(H.pylori)infection is highly prevalent in the human population and may lead to severe gastrointestinal pathology including gastric and duodenal ulcers,mucosa associated tissue lymphoma and gastric adenocarcinoma.In recent years,an alarming increase in antimicrobial resistance and subsequently failing empiric H.pylori eradication therapies have been noted worldwide,also in many European countries.Therefore,rapid and accurate determination of H.pylori’s antibiotic susceptibility prior to the administration of eradication regimens becomes ever more important.Traditionally,detection of H.pylori and its antimicrobial resistance is done by culture and phenotypic drug susceptibility testing that are cumbersome with a long turn-around-time.Recent advances in diagnostics provide new tools,like real-time polymerase chain reaction(PCR)and line probe assays,to diagnose H.pylori infection and antimicrobial resistance to certain antibiotics,directly from clinical specimens.Moreover,high-throughput whole genome sequencing technologies allow the rapid analysis of the pathogen’s genome,thereby allowing identification of resistance mutations and associated antibiotic resistance.In the first part of this review,we will give an overview on currently available diagnostic methods for detection of H.pylori and its drug resistance and their implementation in H.pylori management.The second part of the review focusses on the use of next generation sequencing technology in H.pylori research.To this end,we conducted a literature search for original research articles in English using the terms“Helicobacter”,“transcriptomic”,“transcriptome”,“next generation sequencing”and“whole genome sequencing”.This review is aimed to bridge the gap between current diagnostic practice(histology,rapid urease test,H.pylori culture,PCR and line probe assays)and new sequencing technologies and their potential implementation in diagnostic laboratory settings in order to complement the currently recommended H.pylori management guidelines and subsequently improve public health. | Daniel Pohl Peter M Keller Valentine Bordier Karoline Wagner | 2019 | World Journal of Gastroenterology2019，25，32： | 15 |
| 2 | New aspects in the pathomechanism and diagnosis of the laryngopharyngeal reflux-clinical impact of laryngeal proton pumps and pharyngeal p H metry in extraesophageal gastroesophageal reflux disease显示文摘AIM:To determine the laryngeal H+K+-ATPase and pharyngeal p H in patients with laryngopharyngeal reflux(LPR)-symptoms as well as to assess the symptom scores during PPI therapy.METHODS:Endoscopy was performed to exclude neoplasia and to collect biopsies from the posterior cricoid area(immunohistochemistry and PCR analysis).Immunohistochemical staining was performed with monoclonal mouse antibodies against human H+K+-ATPase.Quantitative real-time RT-PCR for each of the H+K+-ATPase subunits was performed.The p H values were assessed in the aerosolized environment of the oropharynx(Dxp H Catheter) and compared to a subsequently applied combined p H/MII measurement.RESULTS:Twenty patients with LPR symptoms were included.In only one patient,the laryngeal H+K+-ATPase was verified by immunohistochemical staining.In another patient,real-time RT-PCR for each H+K+-ATPase subunit was positive.Fourteen out of twenty patients had pathological results in Dxp H,and 6/20 patients had pathological results in p H/MII.Four patients had pathological results in both functional tests.Nine out of twenty patients responded to PPIs.CONCLUSION:The laryngeal H+K+-ATPase can only be sporadically detected in patients with LPR symptoms and is unlikely to cause the LPR symptoms.Alternative hypotheses for the pathomechanism are needed.The role of pharyngeal p H-metry remains unclearand its use can only be recommended for patients in a research study setting. | Valentin Becker Romina Drabner Simone Graf Christoph Schlag Simon Nennstiel Anna Maria Buchberger Roland M Schmid Dieter Saur Monther Bajbouj | 2015 | World Journal of Gastroenterology2015，21，3： | 14 |
| 3 | Antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis显示文摘Objective:To explore antibacterial activity and characterization of secondary metabolites isolated from mangrove plant Avicennia officinalis(A.officinalis).Methods:In the present study the leaf extracts of A.officinalis were examined for its antibacterial potential using five different solvents against some reference strains of human pathogenic bacteria for the crude extract.Maximum activity was observed for ethyl acetate and hence different concentrations like 15μL,25μL,and 50μL of ethyl extracts was checked for its antibacterial activity.Partial purification of crude extract was carried by column chromatography and fractions were analyzed using gas chromatography-mass spectrometry(GC-MS) to identify compounds.Results:The crude ethyl acetate extracts of A.officinalis showed remarkable antibacterial activity with zones of inhibition of 13 mm against Eschericia coli(E.coli) and 11 mm against Staphylococcus aureus (S.aureus).Fraction 13(ethyl acetate:methanol= 8:2) as the most potent one against with the minimal inhibitory concentration of 30 mm against E.coli and 25 mm against S.aureus.The GCMS resultsof active column fraction(F13) revealed that the active principals were a mixture of hydroxy- 4 methoxybenzoic acid,diethyl phthalate,oleic acid.Conclusions:The leaf extracts with proven antibacterial effects can clearly be directed towards cancer treatment as to inhibiting cancer cell growth.The limited number of test organisms owes to a constraint of resource.So,the effect of strong bursts of leaf extracts on human pathogenic bacteria should further be tested on a wide range of test organisms. | Valentin Bhimba B J Meenupriya Elsa Lycias Joel D Edaya Naveena Suman kumar M Thangaraj | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，7： | 13 |
| 4 | Influence of experimental soil disturbances on the diversity of plants in agricultural grasslands显示文摘Aims and Methods Disturbance is supposed to play an important role for biodiversity and ecosystem stability as described by the intermediate disturbance hypoth-esis(IDH),which predicts highest species richness at intermediate levels of disturbances.In this study,we tested the effects of artificial soil distur-bances on diversity of annual and perennial vascular plants and bryo-phytes in a field experiment in 86 agricultural grasslands differing in land use in two regions of Germany.On each grassland,we implemented four treatments:three treatments differing in application time of soil dis-turbances and one control.One year after experimental disturbance,we recorded vegetation and measured biomass productivity and bare ground.We analysed the disturbance response taking effects of region and land-use-accompanied disturbance regimes into account.Important Findings Region and land-use type strongly determined plant species rich-ness.Experimental disturbances had small positive effects on the species richness of annuals,but none on perennials or bryophytes.Bare ground was positively related to species richness of bryophytes.However,exceeding the creation of 12%bare ground further distur-bance had a detrimental effect on bryophyte species richness,which corresponds to the IDH.As biomass productivity was unaffected by disturbance our results indicate that the disturbance effect on spe-cies richness of annuals was not due to decreased overall produc-tivity,but rather due to short-term lowered inter-and intraspecific competition at the newly created microsites.Generally,our results highlight the importance of soil disturbances for species richness of annual plants and bryophytes in agricultural grasslands.However,most grasslands were disturbed naturally or by land-use practices and our additional experimental soil dis-turbances only had a small short-term effect.Overall,total plant diversity in grasslands seemed to be more limited by the availabil-ity of propagules rather than by suitable microsites for germination.Thus,nature conservation efforts to increase grassland diversity should focus on overcoming propagule limitation,for instance by additional sowing of seeds,while the creation of additional open patches by disturbance might only be appropriate where natural disturbances are scarce. | Jörg Müller Johannes Heinze Jasmin Joshi Steffen Boch Valentin H.Klaus Markus Fischer Daniel Prati | 2014 | Journal of Plant Ecology2014，7，6： | 3 |
| 5 | Alterations in the PITSLRE protein kinase gene complex on chromosome P36 in childhood neuroblastoma显示文摘 | Lahit JM Valentine M Xiang J | 1994 | Nat Genet1994，7，： | 2 |
| 6 | Fusion of a kinase gene, ALK, to a nucleolar protein gene, NPM, in non-Hodgkin′s lymphoma显示文摘 | Morris S W Kirstein M N Valentine M B | 1994 | Science1994，263，5151： | 1 |
| 7 | Residents' Perceptions of Community Tourism Impacts 显示文摘 | ANDERECK K L VALENTINE K M KNOPF R C | 2005 | Annals of Tourism Research2005，32，4： | 1 |
| 8 | Sampling methods to estimate foliage and other characteristics of individual trees显示文摘 | Grregoige T G Valentine H T Fumival G M | 1995 | Ecology1995，，76： | 1 |
| 9 | Prehospital cardiac arrest treated urban first responders:profile of patient response and prediction of outcome by ventricular fibrillation waveform显示文摘 | Callaham M Braun O Valentine W | 1993 | Ann Emerg Med1993，22，11： | 1 |
| 10 | Evaluation of the long-term cost-effectiveness of insulin detemir compared with neutral protamine hagedorn insulin in patients with type 1 diabetes using a basal-bolus regimen in Sweden显示文摘 | Valentine WJ Aagren M Haglund M | 2011 | ScandJ Public Health2011，39，1： | 1 |
| 11 | Molecular organiza?tion of the cell wall of Candida albicans and its relation to pathogenici?ty显示文摘 | Ruiz- HerreraJ Elorza M V Valentin E | 2006 | FEMS Yeast Res2006，6，1： | 1 |
| 12 | The response of straight mobile bed hannels to inbank and overbank flows 显示文摘 | Knight D W Brown F A Valentine E M | 1999 | Proc Instn of Civil Engineers Water Maritime and Energy Division London1999，136，4： | 1 |
| 13 | Primary prevention of cardiovascular disease with atorvastatin in type 2 diabetes in the Collaborative Atorvastatin Diabetes Study (CARDS): multicentre randomised placebo-controlled trial显示文摘 | Helen M Colhoun D John Betteridge Paul N Durrington Graham A Hitman H Andrew W Neil Shona J Livingstone Margaret J Thomason Michael I Mackness Valentine Charlton-Menys John H Fuller | 2004 | The Lancet . 2004 (9435)2004，，9435： | 1 |
| 14 | Mechanisms involved in the conduction of anterior temporal epileptiform discharges to the scalp显示文摘 | Sparkes M Valentin A Alarcon G | 2009 | Clin Neurophysiol2009，120，12： | 1 |
| 15 | Patient sety in intensive care: results from the multinational Sentinel Events Evaluation (SEE) study显示文摘 | Valentin A Capuzzo M Guidet B | 2006 | Intensive care medicine2006，32，10： | 1 |
| 16 | Chronic unpredictable str- ess decreases cell proliferation in the cerebra/ cortex of the adult rat 显示文摘 | Banasr M Valentine GW Li XY | 2007 | Biol Psychiatry2007，62，5： | 1 |
| 17 | Free sterol compositions from the sea cucumbers Pseudostichopus tra- chus , Holothuria (Microtele) nobilis , Holothuria scabra, Trochos- toma orientale and Bathyplotes natans 显示文摘 | VALENTINE A S LJUDMILA P P TATIANA N M | 1998 | Comp Biochem Phys B1998，120，2： | 1 |
| 18 | Natural rubber/clay nanocomposites: Influence of poly(ethylene glycol) on the silicate dispersion and local chain order of rubber network显示文摘 | Carretero-Gonzalez J Valentin J L Arroyo M | 2008 | European Polymer Journal2008，44，11： | 1 |
| 19 | Isotopic evidence for the incorporation of methane-derived carbon intoforaminifera from modern methane seeps, Hydrate Ridge, Northeast Paeific 显示文摘 | Hill T M Kenett J P Valentine D L | 2004 | Geochimica et Cosmochimica Acta2004，68，22： | 1 |
| 20 | Radiological and neurological changes in the drug abuse patient: a study with MRI显示文摘 | Volkow ND Valentine A Kulkami M | 1998 | Neuroradiol1998，15，3： | 1 |
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