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| 1 | A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity显示文摘The complete genome of Marek's disease virus (MDV) strain GX0101, which was integrated with the LTR sequences of REV, was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the US2 locus of the MDV genome by homologous recombination. The viral DNA containing the BAC vector was used to transform Escherichia coli strain of DH10B. Then the recombinant virus was successfully rescued by transfection of the recombinant BAC DNA into primary chicken embryo fibroblast (CEF). This BAC viral clone was named bac-GX0101. When the reconstituted virus was inoculated into 1-day-old birds, visceral tumors could be detected as early as 62 d post infection. There was no difference in growth ability and pathogenicity to birds between the BAC derived virus and its parental virus. The BAC derived virus maintained its oncogenicity and immunosuppressive effects. In conclusion, the complete genome of GX0101 strain was successfully cloned into BAC and the infectious clone was rescued. With the powerful BAC manipulation system, the infectious clone will provide a useful tool for further understanding the functional roles of the inserted REV-LTR sequence in the GX0101 strain of MDV. | SUN AiJun LAWRENCE Petherbridge ZHAO YuGuang LI YanPeng NAIR Venugopal K CUI ZhiZhong | 2009 | Chinese Science Bulletin2009，54，15： | 15 |
| 2 | 带有REV-LTR片段的MDV野毒株GX0101 BAC克隆的构建及拯救病毒的致病性分析显示文摘将整合有禽网状内皮组织增生症病毒(reticuloendotheliosis,REV)长末端重复序列(LTR)的马立克氏病病毒(命名为GX0101)全基因组作为细菌人工染色体(bacterial artificial chromosome,BAC)克隆进大肠杆菌.BAC载体通过同源重组插入MDV基因组的US2区,含BAC载体的MDV DNA电转化大肠杆菌菌株DH10B,鉴定含MDV全基因组的BAC克隆(MDV BAC),提取BAC DNA,转染原代鸡胚成纤维细胞(CEF),成功拯救出了重组病毒,命名为bac-GX0101.将此病毒腹腔接种1日龄鸡,在攻毒后62d开始检测到内脏肿瘤,动物实验结果表明,bac-GX0101毒株与亲本GX0101毒株对机体的致病性等方面的影响没有差异,bac-GX0101毒株仍然保留着很好的免疫抑制功能及致肿瘤能力.该感染性克隆bac-GX0101为研究该重组野毒株中REV-LTR插入片段及其他基因的生物学功能提供了有用的技术平台. | 孙爱军 PETHERBRIDGE Lawrence ZHAO YuGuang 李延鹏 NAIR Venugopal K 崔治中 | 2009 | 科学通报2009，54，11： | 7 |
| 3 | Flexible SERS substrates for hazardous materials detection: recent advances显示文摘This article reviews the most recent advances in the development of flexible substrates used as surface-enhanced Ra-man scattering(SERS)platforms for detecting several hazardous materials(e.g.,explosives,pesticides,drugs,and dyes).Different flexible platforms such as papers/filter papers,fabrics,polymer nanofibers,and cellulose fibers have been investigated over the last few years and their SERS efficacies have been evaluated.We start with an introduction of the importance of hazardous materials trace detection followed by a summary of different SERS methodologies with par-ticular attention on flexible substrates and their advantages over the nanostructures and nanoparticle-based solid/hybrid substrates.The potential of flexible SERS substrates,in conjunction with a simple portable Raman spectrometer,is the power to enable practical/on-field/point of interest applications primarily because of their low-cost and easy sampling. | Moram Sree Satya Bharati Venugopal Rao Soma | 2021 | Opto-Electronic Advances2021，4，11： | 6 |
| 4 | Coal washing scenario in India and future prospects显示文摘 | R. Venugopal J. P. Patel C. Bhar | 2016 | International Journal of Coal Science & Technology2016，3，2： | 5 |
| 5 | Cloud computing and emerging IT platforms: Vision, hype, and reality for delivering computing as the 5th utility显示文摘 | Rajkumar Buyya Chee Shin Yeo Srikumar Venugopal James Broberg Ivona Brandic | 2008 | Future Generation Computer Systems2008，，6： | 5 |
| 6 | 妊娠性高血压视网膜变化的临床研究(英文)显示文摘目的:研究妊娠性高血压(PIH)患者视网膜变化的患病率,视网膜变化与疾病严重程度的相关性。方法:横断面研究。共收集2014-12/2015-05妊娠性高血压患者100例。所有患者均行眼底检查。单眼或双眼眼底发生变化视为阳性。妊娠性高血压分级为轻度子痫前期(血压>140/90~160/90 mmHg),重度子痫前期(血压>160/90 mmHg)和子痫前期(重度子痫前期和抽搐)。结果:患者平均年龄为23.05(19~34)岁。妊娠期为28~41wk。其中62例初产妇以及38例经产妇。轻度子痫前期,重度子痫前期和子痫前期患者占比分别为62%,26%,12%。8例患者发生视网膜变化。观察得出:高血压性视网膜病变Ⅰ级(1%),Ⅱ级(0%),Ⅲ级(2%),Ⅳ级(2%),浆液性视网膜脱离(1%)和中心性浆液性脉络膜视网膜病变(2%)。视网膜变化与PIH严重程度呈正相关,有统计学意义(P=0.0001)。结论:8%的PIH患者视网膜发生变化,视网膜变化与PIH严重程度呈正相关。 | sahana raviraj manipur pavana acharya kavitha chikkanayakanahalli venugopal lakshmi bomalapura ramamurthy | 2017 | 国际眼科杂志2017，17，6： | 4 |
| 7 | Phytochemicals in diets for breast cancer prevention:The importance of resveratrol and ursolic acid显示文摘Breast cancer is the second leading cause of death from cancer in women in the United States.A growing emphasis is being placed on alternative medicine and dietary approaches toward prevention of potential diseases.Phytochemicals are bioactive compounds that are naturally present in foods that,when acting in synergy,bestow potential anti-cancer properties.Resveratrol,a phytoalexin,and ursolic acid,a pentacyclic triterpenoid,are two bioactive compounds that are at the forefront in scientific research.Previous animal studies have documented the anti-cancer properties of resveratrol on breast cancer cells and research groups have recently been able to identify the anti-cancer,anti-inflammatory and induction of apoptosis properties of resveratrol along with the signal transduction pathways that the compound affects.Ursolic acid has been cast into the limelight with the recent discovery documenting its anti-inflammation and anti-cancer activities by targeting signal pathways,especially in the prevention of breast cancer.©2012 Production and hosting by Elsevier B.V.on behalf of Beijing Academy of Food Sciences. | Rahul Venugopal Rui Hai Liu | 2012 | Food Science and Human Wellness2012，1，1： | 3 |
| 8 | Sequential disruption of ALV host receptor genes reveals no sharing of receptors between ALV subgroups A, B, and J显示文摘Background: Previously, we showed that targeted disruption of viral receptor genes in avian leukosis virus(ALV)subgroups using clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein 9(Cas9))-based genome editing confers resistance to ALV subgroups B and J. Here, we used the same strategy to target the receptor expressed by ALV subgroup A(TVA) and generate chicken cells resistant to infection by this virus.Results: CRISPR/Cas9-based disruption of exon 2 within the tva gene of DF-1 fibroblasts conferred resistance to infection by ALV subgroup A regardless of whether frameshift mutations were introduced during editing. Conversely,overexpression of the wild-type TVA receptor(wtTVA) by tva-modified DF-1 clones restored susceptibility to ALV subgroup A. The results confirm that exon 2, which contains the low-density lipoprotein receptor class A domain of TVA, is critical for virus entry. Furthermore, we sequentially modified DF-1 cells by editing the tva, tvb, and Na^+/H^+ exchange 1(chNHE1) genes, which are the specific receptors for ALV subgroups A, B, and J, respectively.Conclusions: Simultaneous editing of multiple receptors to block infection by different subgroups of ALV confirmed that ALV subgroups A, B, and J do not share host receptors. This strategy could be used to generate cells resistant to multiple viral pathogens that use distinct receptors for cell entry. | Hong Jo Lee Kyung Je Park Kyung Youn Lee Yongxiu Yao Venugopal Nair Jae Yong Han | 2019 | Journal of Animal Science and Biotechnology2019，10，2： | 3 |
| 9 | Microstructural Characterization and Mechanical Properties of Powder Metallurgy Dual Phase Steel Preforms显示文摘In order to improve the mechanical properties of powder metallurgy (P/M) ferrite-pearlite steel, a dual phase (DP) ferrite-martensite steel was produced through intercritical annealing of sintered P/M preforms. Mi-crostructures of the sintered and DP steels were examined with optical, scanning and transmission electron microscopes. Mechanical properties were evaluated through hardness measurements and compression tests. Microstructural studies revealed that sintered steel contained polygonal ferrite-pearlite while the DP steel contained polygonal, lath and acicular ferrite along with lath-type martensite as microstructural constituents. In DP steels, with increasing mean preform density, the microstructure contained fine and continuous network of martensite colonies with minimum porosity. The work hardening rate vs plastic strain plots (Jaoul-Crussard analysis) of both the steels revealed typical three stage deformation behaviour for low and high mean preform densities. Compression tests revealed that, DP P/M steel displayed higher strength-plasticity combination than the sintered steel. | K. Mahesh S. Sankaran P. Venugopal | 2012 | Journal of Materials Science & Technology2012，28，12： | 3 |
| 10 | Effect of C-reactive protein on vascular cells: evidence for a proinflammatory, proatherogenic role显示文摘 | Senthil Kumar Venugopal Sridevi Devaraj Ishwarlal Jialal | 2005 | Current Opinion in Nephrology and Hypertension2005，，1： | 3 |
| 11 | Characteristics of La-modified Ni-Al_2O_3 and Ni-SiO_2 catalysts for CO_x-free hydrogen production by catalytic decomposition of methane显示文摘Hydrotalcite precursors of La modified Ni-Al2O3 and Ni-SiO2 catalysts prepared by co-precipitation method and the catalytic activities were examined for the production of COx-free H2 by CH4 decomposition. Physico-chemical characteristics of fresh, reduced and used catalysts were evaluated by XRD, TPR and O2 pulse chemisorptions, TEM and BET-SA techniques. XRD studies showed phases due to hydrotalcite-like precursors in oven dried form produced dispersed NiO species upon calcination in static air above 450 C. Raman spectra of deactivated samples revealed the presence of both ordered and disordered forms of carbon. Ni-La-Al2O3catalyst with a mole ratio of Ni : La : Al = 2 : 0.1 : 0.9 exhibited tremendously high longevity with a hydrogen production rate of 1300 molH2 mol 1 Ni. A direct relationship between Ni metal surface area and hydrogen yields was established. | Chatla Anjaneyulu Velisoju Vijay Kumar Suresh K.Bhargava Akula Venugopal | 2013 | Journal of Energy Chemistry2013，22，6： | 2 |
| 12 | Electrospinning of curcumin loaded chitosan/poly (lactic acid) nanofilm and evaluation of its medicinal characteristics显示文摘curcumin 装载了 chitosan/poly (乳的酸)(PLA ) nanofibers 用 electrospinning 被生产。BoxBehnken 试验性的设计被用于变量的优化(1, 0, + 1 编码水平) 相似 chitosan/PLA 力量(% w/v ) , curcumin 力量(% w/v ) 和应用电压(kV ) 到获得一致纤维直径。nanofibers 的形态学被 SEM 显示出。分子的相互作用和 curcumin 的各化学的化合物的存在装载了 chitosan/PLA 纤维被 FTIR 和 EDX 分析描绘。抗氧化剂,药版本并且在 vitro cytotoxicity,测试被执行评估将被用于创伤愈合的 nanofibers 的适用性。在愈合弯屈在切除和切口上学习的 vivo,创伤在给创伤区域的重要减小看是否的老鼠模型上创造了与相比未经治疗。更好愈合的效率能被归因于 curcumin 和 chitosan 的存在。 | Bhaarathi DHURAI Nachimuthu SARASWATHY Ramasamy MAHESWARAN Ponnusamy SETHUPATHI Palanisamy VANITHA Sukumar VIGNESHWARAN~, and Venugopal RAMESHBABU | 2013 | Frontiers of Materials Science2013，7，4： | 2 |
| 13 | Phylogenetic analysis and protective effects of thymol and its chromatographic fractions from a novel wild mushroom in combating oxidative stress显示文摘Mushrooms are good sources of phytochemicals that have antioxidant and anti-proliferative effects.This study identifi ed a unique isoform of 18S rRNA gene(864 bp)from a novel wild mushroom(SMK-1)(GenBank accession number:SUB3267363).Thin layer chromatographic(TLC)profiling of the methanolic extract of the dried mushroom fruiting bodies of SMK-1 revealed the presence of phenolic and fl avonoid fractions with retention factor(Rf)values of 0.955 and 0.927 respectively.The GC/MS chromatograms of the SMK-1 methanolic extract identifi ed the main bioactive compound was phenol,5-methyl-2-(1-methylethyl)(74.00%)(thymol).The radical scavenging activity for the fl avonoid fraction was greater than the phenolic fractions(Rf–phenolics fractions>Rf–fl avonoid fractions)with the antioxidant activity more than that of standard ascorbic acid.Also,the phenolic and flavonoid fractions of SMK-1 expressed cytotoxic effects in HeLa cells with IC50 values ranging from 5μg/mL to 80μg/mL in a dose-dependent manner.This present research highlights the presence of high thymol concentration in a novel wild mushroom that has antioxidant and anti-poliferative potential with therapeutic benefi ts.The application of thymol natural products from novel mushroom SMK-1 as nutrition supplements could inhibit oxidative stress triggered by numerous pathologies that may pave the way to develop a new therapeutic natural drug. | M.Subbulakshmi Sugapriya Dhanasekaran S.Abirami M.Kannan R.Palaniappan Divya Venugopal | 2021 | Food Science and Human Wellness2021，10，4： | 2 |
| 14 | 纤维蛋白胶与无缝无胶自体球结膜瓣固定术治疗翼状胬肉的疗效比较(英文)显示文摘目的:比较两种大小纤维蛋白胶与无缝无胶自体球结膜瓣固定术在原发性翼状胬肉手术中的患者舒适度、移植物稳定性、移植物炎症、复发等术后并发症。方法:本研究对2014-12/2016-08共80例翼状胬肉患者(80眼)进行前瞻性介入对比研究。研究组随机抽取并分为两组,组1接受纤维蛋白胶疗法,组2采用无缝合无胶结膜自体移植固定术。每组再将等量的受试者随机平均分为两组(组1:组A+组B,组2:组C+组D)。组A(自体移植物的大小与裸眼巩膜相同)和组B(比裸巩膜大1 mm)实施纤维蛋白胶疗法;组C(自体移植物的大小与裸眼巩膜相同)和组D(比裸巩膜大1 mm)采用无缝线无胶结膜自体移植固定术。每一位患者都接受翼状胬肉切除术,并根据组别使用不同大小的自体球结膜瓣。结果:组1平均年龄为43.525,组2平均年龄为42.2。男性20例,女性60例。37例患者右眼有翼状胬肉。四组间平均手术时长相似。在术后随访期间,除结膜下出血(纤维蛋白胶组在术后1wk内明显增多)外,4组间患者的舒适度、移植物回缩、移植物炎症和移植物水肿等术后参数均相似(P<0.0001)。组2仅3例患者出现4级移植物回缩/移位。术后1wk,组A和组D各出现1例移植囊肿患者。随访6mo后,组D中2例患者复发。结论:两种方法均安全有效,术后不适及并发症少。与纤维蛋白胶疗法相比,无缝无胶疗法更加经济且效果相当。由于该手术成本低,且具有纤维蛋白胶疗法的所有优点,无缝无胶法可被认为是治疗患者非常有效的方法。 | Manipur Raviraj Sahana Chikkanayakanahalli Venugopal Kavitha Acharya Pavana Bomalapura Ramamurthy Lakshmi | 2019 | 国际眼科杂志2019，19，11： | 2 |
| 15 | Cardiogenic differentiation of mesenchymal stem cells on elastomeric poly (glycerol sebacate)/collagen core/shell fibers显示文摘AIM: To facilitate engineering of suitable biomaterials to meet the challenges associated with myocardial infarction. METHODS: Poly (glycerol sebacate)/collagen (PGS/ collagen) core/shell fibers were fabricated by core/ shell electrospinning technique, with core as PGS and shell as collagen polymer; and the scaffolds were characterized by scanning electron microscope (SEM), fourier transform infrared spectroscopy (FTIR), contact angle and tensile testing for cardiac tissue engineering. Collagen nanofibers were also fabricated by electrospinning for comparison with core/shell fibers. Studies on cell-scaffold interaction were carriedout using cardiac cells and mesenchymal stem cells (MSCs) co-culture system with cardiac cells and MSCs separately serving as positive and negative controls respectively. The co-culture system was characterized for cell proliferation and differentiation of MSCs into cardiomyogenic lineage in the co-culture environment using dual immunocytochemistry. The co-culture cells were stained with cardiac specific marker proteins like actinin and troponin and MSC specific marker protein CD 105 for proving the cardiogenic differentiation of MSCs. Further the morphology of cells was analyzed using SEM.RESULTS: PGS/collagen core/shell fibers, core is PGS polymer having an elastic modulus related to that of cardiac fibers and shell as collagen, providing natural environment for cellular activities like cell adhesion, proliferation and differentiation. SEM micrographs of electrospun fibrous scaffolds revealed porous, beadless, uniform fibers with a fiber diameter in the range of 380 ± 77 nm and 1192 ± 277 nm for collagen fibers and PGS/collagen core/shell fibers respectively. The obtained PGS/collagen core/shell fibrous scaffolds were hydrophilic having a water contact angle of 17.9 ± 4.6° compared to collagen nanofibers which had a contact angle value of 30 ± 3.2°. The PGS/collagen core/shell fibers had mechanical properties comparable to that of native heart muscle with a young's modulus of 4.24 ± 0.7 MPa, while that of collagen nanofibers was comparatively higher around 30.11 ± 1.68 MPa. FTIR spectrum was performed to confirm the functional groups present in the electrospun scaffolds. Amide Ⅰ and amide Ⅱ of collagen were detected at 1638.95 cm -1 and 1551.64 cm -1 in the electrospun collagen fibers and at 1646.22 cm -1 and 1540.73 cm -1 for PGS/collagen core/shell fibers respectively. Cell culture studies performed using MSCs and cardiac cells co-culture environment, indicated that the cellproliferation significantly increased on PGS/collagen core/shell scaffolds compared to collagen fibers and the cardiac marker proteins actinin and troponin were expressed more on PGS/collagen core/shell scaffolds compared to collagen fibers alone. Dual immunofluorescent staining was performed to further confirm the cardiogenic differentiation of MSCs by employing MSC specific marker protein, CD 105 and cardiac specific marker protein, actinin. SEM observations of cardiac cells showed normal morphology on PGS/collagen fibers and providing adequate tensile strength for the regeneration of myocardial infarction. CONCLUSION: Combination of PGS/collagen fibers and cardiac cells/MSCs co-culture system providing natural microenvironments to improve cell survival and differentiation, could bring cardiac tissue engineering to clinical application. | Rajeswari Ravichandran Jayarama Reddy Venugopal Subramanian Sundarrajan Shayanti Mukherjee Seeram Ramakrishna | 2013 | World Journal of Cardiology2013，5，3： | 2 |
| 16 | AC impedance study on the activation mechanism of aluminium by indium and zinc in 3.5% NaCl medium显示文摘 | A. Venugopal V.S. Raja | 1997 | Corrosion Science1997，，12： | 2 |
| 17 | Evaluation of arc sprayed coatings for erosion protection of tubes in atmospheric fluidised bed combustion (AFBC) boilers显示文摘 | Krishnarao Venugopal Manish Agrawal | 2007 | Wear2007，，1： | 2 |
| 18 | Production of ultrafine grain sizes in aluminium sheets by severe plastic deformation using the technique of groove pressing显示文摘 | A. Krishnaiah Uday Chakkingal P. Venugopal | 2005 | Scripta Materialia2005，，12： | 2 |
| 19 | Influence of Rotational Speed on Microstructure and Mechanical Properties of Dissimilar Metal AISI 304-AISI 4140 Continuous Drive Friction Welds显示文摘Fundamental investigation of continuous drive friction welding of austenitic stainless steel (AISI 304) and low alloy steel (AISI 4140) is described. The emphasis is made on the influence of rotational speed on the microstructure and mechanical properties such as hardness, tensile strength, notch tensile strength and impact toughness of the dissimilar joints. Hardness profiles across the weld show the interface is harder than the respective parent metals. In general, maximum peak hardness is observed on the stainless steel side, while other peak hardness is on the low alloy steel side. A trough in hardness distribution in between the peaks is located on the low alloy steel side. Peak hardness on the stainless steel and low alloy steel side close to the interface increases with a decrease in rotational speed. All transverse tensile joints fractured on stainless steel side near the interface. Notch tensile strength and impact toughness increase with increase in rotational speed up to 1 500 r/min and decrease thereafter. The mechanism of influence of rotational speed for the observed trends is discussed in the torque, displacement characteristics, heat generation, microstructure, fractography and mechanical properties. | G Subhash Chander G Madhusudhan Reddy A Venugopal Rao | 2012 | Journal of Iron and Steel Research(International)2012，19，10： | 2 |
| 20 | 氧化铝和氧化锆催化剂上2-取代苯并咪唑和1,5-二取代苯并二氮的合成(英文)显示文摘In this study, alumina, zirconia, manganese oxide/alumina, and manganese oxide/zirconia have been investigated for their catalytic activity in the condensation reaction between o-phenylenediamine and an aldehyde or a ketone to synthesise 2-substituted benzimidazoles and 1,5-disubstituted benzodiazepines respectively. Surface area, surface acidity, and morphology of the catalysts have been analysed and correlated with their catalytic activity. The isolated yields of 2-substituted benzimidazoles and 1,5-disubstituted benzodiazepines are in the range of 30% to 95%. A good correlation between the amount of surface acid sites as well as the surface morphology of the catalysts and the catalytic activity has been observed. This method has been found to be simple and economical. The solid supports could be regenerated and reused without much loss in their activity. Further, the solid supports have been also found to be effective as general catalysts in the condensation of o-phenylenediamine with other substituted aldehydes and ketones. | M. REKHA A. HAMZA B. R. VENUGOPAL N. NAGARAJU | 2012 | 催化学报2012，33，3： | 2 |
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