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2篇 您的检索式:作者名="V. K. Varshney"
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1Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species显示文摘Ramesh K. Aggarwal Prasad S. Hendre Rajeev K. Varshney Prasanna R. Bhat V. Krishnakumar Lalji Singh 2007Theoretical and Applied Genetics2007,,2:2
2Population genetic structure and diversity of high value vulnerable medicinal plant Acorus calamus in India using RAPD and chloroplast microsatellite markers显示文摘Acorus calamus is a highly valued medicinal plant with global distribution used in several drugs of health care systems. We evaluated the genetic diversity and population structure of 50 populations of A. calamus from different geographical regions in India through RAPD and chloroplast microsatellite markers. From the total screened 82 RAPD primers and 18 cpSSR primers, 10 RAPD and nine cpSSRs were found polymorphic. The selected 10 RAPD primers produced a total of 96 reproducible bands, out of which 65 were polymorphic (67.70%). Whereas, the selected nine cpSSR markers produced 26 alleles and all of them were polymorphic. The mean genetic diversity (H T ) among populations using RAPD (H T = 0.263) and cpSSR (H T = 0.530) markers was higher in comparison to the mean genetic diversity within populations. Mean coefficient of gene differentiation (G ST ) between the populations was also high for both RAPD (G ST = 0.830) and cpSSR markers (G ST = 0.735), whereas the estimated gene flow was very low for RAPD (Nm = 0.102) and for cpSSR (Nm = 0.179). AMOVA analysis revealed that more genetic variation resided among the populations than within populations. Significant differences (p < 0.001) were observed between the populations and individuals within the populations. Cluster analysis of RAPD and cpSSR data using UPGMA algorithm based on Nei's genetic similarity matrix placed the 50 populations into two main clusters. The implication of the results of this study in devising strategy for conservation of A. calamus is discussed.H. S. Ginwal Neha Mittal Arvind Tomar V. K. Varshney 2011Journal of Forestry Research2011,22,3:0
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