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| 1 | A Rapid and Cost-Effective Method for Genotyping Genome-Edited Animals:A Heteroduplex Mobility Assay Using Microfluidic Capillary Electrophoresis显示文摘The recent emergence and application of engineered endonucleases have led to the development of genome editing tools capable of rapidly implementing various targeted genome editions in a wide range of species.Moreover,these novel tools have become easier to use and have resulted in a great increase of applications.Whilst gene knockout(KO) or knockin(KI) animal models are relatively easy to achieve,there is a bottleneck in the detection and analysis of these mutations.Although several methods exist to detect these targeted mutations,we developed a heteroduplex mobility assay on an automated microfluidic capillary electrophoresis system named HMA-CE in order to accelerate the genotyping process.The HMA-CE method uses a simple PCR amplification of genomic DNA(gDNA) followed by an automated capillary electrophoresis step which reveals a heteroduplexes(HD) signature for each mutation.This allows efficient discrimination of wild-type and genome-edited animals down to the single base pair level. | Vanessa Chenouard Lucas Brusselle Jean-Marie Heslan Séverine Remy Séverine Ménoret Claire Usal Laure-Hélène Ouisse Tuan Huy NGuyen Ignacio Anegon Laurent Tesson | 2016 | Journal of Genetics and Genomics2016，43，5： | 4 |
| 2 | Characterization of two rat models of cystic fibrosis—KO and F508del CFTR—Generated by Crispr-Cas9显示文摘Background: Genetically engineered animals are essential for gaining a proper understanding of the disease mechanisms of cystic fibrosis(CF). The rat is a relevant laboratory model for CF because of its zootechnical capacity, size, and airway characteristics, including the presence of submucosal glands.Methods: We describe the generation of a CF rat model(F508 del) homozygous for the p.Phe508 del mutation in the transmembrane conductance regulator(Cftr) gene. This model was compared to new Cftr-/-rats(CFTR KO). Target organs in CF were examined by histological staining of tissue sections and tooth enamel was quantified by micro-computed tomography. The activity of CFTR was evaluated by nasal potential difference(NPD) and short-circuit current measurements. The effect of VX-809 and VX-770 was analyzed on nasal epithelial primary cell cultures from F508 del rats.Results: Both newborn F508 del and Knock out(KO) animals developed intestinal obstruction that could be partly compensated by special diet combined with an osmotic laxative. The two rat models exhibited CF phenotypic anomalies such as vas deferens agenesis and tooth enamel defects. Histology of the intestine, pancreas, liver, and lungs was normal. Absence of CFTR function in KO rats was confirmed ex vivo by short-circuit current measurements on colon mucosae and in vivo by NPD, whereas residual CFTR activity was observed in F508 del rats. Exposure of F508 del CFTR nasal primary cultures to a combination of VX-809 and VX-770 improved CFTR-mediated Cl-transport.Conclusions: The F508 del rats reproduce the phenotypes observed in CFTR KO animals and represent a novel resource to advance the development of CF therapeutics. | Elise Dreano Marc Bacchetta Juliette Simonin Louise Galmiche Claire Usal Lotfi Slimani Jérémy Sadoine Laurent Tesson Ignacio Anegon Jean-Paul Concordet Aurélie Hatton Lucile Vignaud Danielle Tondelier Isabelle Sermet-Gaudelus Marc Chanson Charles-Henry Cottart | 2019 | Animal Models and Experimental Medicine2019，2，4： | 3 |
| 3 | Microbiological quality of fresh, minimally-processed fruit and vegetables, and sprouts from retail establishments显示文摘 | M. Abadias J. Usall M. Anguera C. Solsona I. Vi?as | 2008 | International Journal of Food Microbiology2008，，1： | 2 |
| 4 | Improved control of postharvest decay of pears by the combination of Candida sake (CPA 1) and ammonium molybdate 显示文摘 | Nunes C Usall J Teixido N | 2002 | Phytopathology2002，92，： | 1 |
| 5 | Design of protein-targeting system for lactic acid bacteria 显示文摘 | Dieye Y Usal S Clier F | 2001 | J Bact2001，183，14： | 1 |
| 6 | Efficacy of Neutral Electrolyzed Water (NEW) for Reducing Microbial Contamination on Minimally-processed Vegetables显示文摘 | ABADIAS M USALL J OLIVEIRA M | 2008 | Journal of Food Microbiology2008，123，： | 1 |
| 7 | Design of a protein-targeting system for lactic acid bacteria 显示文摘 | Dieye Y Usal S Clier F | 2001 | J Bact2001，183，14： | 1 |
| 8 | Efficacy of neutral electrolyzed water (NEW) for reducing microbial contamination on minimally-processed vegetables显示文摘 | ABADIAS M USALL J OLIVEIRA M | 2008 | International Journal of Food Microbiology2008，123，1： | 1 |
| 9 | Relationship between the incidence of latent infections caused by Monilinia spp . and the incidence of brown rot of peach fruit: factors affecting latent infection显示文摘 | Iray Gell Antonieta Cal Rosario Torres Josep Usall Paloma Melgarejo | 2008 | European Journal of Plant Pathology2008，，4： | 1 |
| 10 | Improving ecological fitness and environmental stress tolerance of the biocontrol yeast Candida sake by manipulation of intraceUuar sugar alcohol and sugar content显示文摘 | TEIXIDO N VINAS I USALL J | 1998 | MycologicalResearch1998，102，11： | 1 |
| 11 | Control of blue mold of apple by preharvest application of Candida sake grown in media with different water activity显示文摘 | TEIXIDO N VINAS I USALL J | 1998 | Phytopathology1998，88，9： | 1 |
| 12 | Antagonistic effect of Pseudomonas graminis CPA-7 against foodbome pathogens in fresh-cut apples under simulated commercial conditions显示文摘 | Alegrc I Vinas I Usall J ct al | 2013 | Food microbiology2013，33，2： | 1 |
| 13 | Effect of protective agents,rehydration media and initial ceil concentration on viability of Pantoea agglomerans strain CPA - 2 subjected to freeze - drying 显示文摘 | Costa E Usall J Teixido N | 2000 | Journal of Applied Microbiology2000，89，： | 1 |
| 14 | Acomparison of conipacl and conventionalring-spun yarns 显示文摘 | YILMAZ DKMKT MUSTAFA KKSIT USAL | 2010 | Textile Kes J2010，11，5： | 1 |
| 15 | Effect of water activity and temperature on competing abilities of common postharvest citrus fungi 显示文摘 | Pilar P Usall J | 2004 | Int J Food Microbiol2004，90，： | 1 |
| 16 | Production of the biocontrol agent Pantoea agglomerans strain CPA-2 using commercial products and by-products显示文摘 | E. Costa N. Teixidó J. Usall E. Atarés I. Vi?as | 2001 | 2001 (3-4)2001，，3： | 1 |
| 17 | Suicide ideation across reproductive life cycle of women Results from a European epidemio-logical study显示文摘 | USALL J PINTO-MEZA A FERN/i NDEZ A | 2009 | J Affect Disord2009，116，12： | 1 |
| 18 | Survival of the postharvest biocontrol yeast Candida sake CPA-1 after dehydration by spray-drying 显示文摘 | ABADIAS M TEIXID N USALL J | 2005 | Bio Sci Technol2005，15，8： | 1 |
| 19 | Effect of biocontrol ageuts Candida sake and Pantoea agglomerans on Penicillium expansum growth and patulin accumulation in apples显示文摘 | MORALES H SANCHIS V USALL J | | 0，，1： | 1 |
| 20 | Renal macrophage activation and TH2 polarization precedes the development of nephrotic syndrome in Buffalo/Mna rats显示文摘 | Le Berre L Herve C Buzelin F Usal C Soulillou JP Dantal J | 2005 | Kidney Int2005，68，5： | 1 |
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