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| 1 | 5-Azacytidine induces changes in electrophysiological properties of human mesenchymal stem cells显示文摘以前,导出髓的干细胞(MSC ) 与不确定的脱氧核糖核酸甲基转移酶禁止者 5-azacytidine 对待的老鼠骨头被报导区分 intocardiomyocytes。现在的学习的目的是在从健康骨髓施主获得的人的单音的原子房间调查类似的区别策略的效率。After1-3 段落,文化为 24 h 暴露于到 6 星期进一步的文化跟随的 5-azacytidine (3 mciroM ) 。药处理没导致 myogenic 标记 MyoD 或心脏的 markersNkx2.5 和 GATA-4 的表示并且没在后续期间产出跳动的房间。在补丁夹钳实验,近似 10-15% 对待,未经治疗的房间展出了 L 类型 Ca (2+) 水流。几乎所有房间表面地出现了修正快速或慢的激活动力学的 K (+) 水流。在与匹配时间的控制相比在 6 星期处理以后加倍的 +60 mV 意味着当前的振幅。对待的房间的 Membranecapacitance 比在在处理以后的 controls2 星期内显著地大并且在 6 个星期以后仍然保持高。为 K (+) 隧道 Kv1.1, Kv1.5, Kv2.1, Kv4.3 和 KCNMA1 并且为 Ca (2+) 隧道 Ca (v) 的 mRNAs 的表示层次 1.2 没被 5-azacytidine 影响。在以前显示出禁止 hMSC 的快速或慢慢地激活的 K (+) 水流的集中的 Treatmentwith 钾隧道块 ers tetraethylammonium 和 clofilium 禁止了这些房间的增长。我们的结果建议尽管有进 cardiomyocytes 的 hMSC 的区别的缺席,有 5-azacytidine 的处理在当前的密度引起了深刻变化。 | Bartosz Balanal Cecilia Nicoletti Ihor Zahanich Eva M Graf Torsten Christ Sabine Boxberger Ursula Ravens | 2006 | Cell Research2006，16，12： | 20 |
| 2 | 金雀异黄素对脂多糖诱导的巨噬细胞MAPK和TLR信号转导通路的影响显示文摘目的研究金雀异黄素对脂多糖(LPS)诱导的巨噬细胞丝裂原活化蛋白激酶(MAPK)信号转导通路和Toll样受体(TLR)通路的影响。方法用100 ng/mL脂多糖和金雀异黄素分别处理RAW264.7巨噬细胞不同时间后,采用Western blot法检测对MAPK信号通路蛋白磷酸化的影响;采用RT2 ProfilerTMPCR芯片检测金雀异黄素对LPS诱导的TLR信号转导通路基因表达的影响。结果 LPS能够显著诱导蛋白p38和p42/44磷酸化,激活MAPK信号通路,金雀异黄素能够加强其作用,同时,LPS能够显著诱导TLR信号转导通路的细胞因子基因表达,包括IFN-β、IL-10、IL-1α、IL-1β、IL-6、TNF-α、集落刺激因子2(CSF-2)、CSF-3、趋化因子CCL2和CXCL10、环氧合酶2(COX-2)、NF-κB1和IκB-α等,金雀异黄素能够显著降低这些上调基因的表达。结论金雀异黄素能够显著增强LPS激活的MAPK信号通路并抑制TLR信号通路的激活。 | 崔树娜 Ursula BILITEWSKI | 2014 | 细胞与分子免疫学杂志2014，30，3： | 10 |
| 3 | Exercise training in hypertension:Role of microRNAs显示文摘Hypertension is a complex disease that constitutes an important public health problem and demands many studies in order to understand the molecular mechanisms involving his pathophysiology. Therefore, an increasing number of studies have been conducted and new therapies are continually being discovered. In this context, exercise training has emerged as an important non-pharmacological therapy to treat hypertensive patients, minimizing the side effects of pharmacological therapies and frequently contributing to allow pharmacotherapy to be suspended. Several mechanisms have been associated with the pathogenesis of hypertension, such as hyperactivity of the sympathetic nervous system and renin-angiotensin aldosterone system,impaired endothelial nitric oxide production, increased oxygen-reactive species, vascular thickening and stiffening, cardiac hypertrophy, impaired angiogenesis, and sometimes genetic predisposition. With the advent of microRNAs(miRNAs), new insights have been added to the perspectives for the treatment of this disease, and exercise training has been shown to be able to modulate the miRNAs associated with it. Elucidation of the relationship between exercise training and miRNAs in the pathogenesis of hypertension is fundamental in order to understand how exercise modulates the cardiovascular system at genetic level. This can be promising even for the development of new drugs. This article is a review of how exercise training acts on hypertension by means of specific miRNAs in the heart, vascular system, and skeletal muscle. | Vander Josédas Neves Tiago Fernandes Fernanda Roberta Roque Ursula Paula RenóSoci Stéphano Freitas Soares Melo Edilamar Menezes de Oliveira | 2014 | World Journal of Cardiology2014，6，8： | 9 |
| 4 | Pulsatile gonadotropin-releasing hormone therapy is associated with earlier spermatogenesis compared to combined gonadotropin therapy in patients with congenital hypogonadotropic hypogonadism显示文摘 | Jiang-Feng Mao Zhao-Xiang Liu Min Nie Xi Wang Hong-Li Xu Bing-Kun Huang Jun-Jie Zheng Le Min Ursula Brigitte Kaiser Xue-Yan Wu | 2017 | Asian Journal of Andrology2017，19，6： | 8 |
| 5 | Mechanisms of hepatic fibrogenesis显示文摘 | Ursula E. Lee Scott L. Friedman | 2011 | Best Practice & Research Clinical Gastroenterology2011，，2： | 7 |
| 6 | 金雀异黄素对脂多糖诱导RAW264.7细胞分泌功能的影响显示文摘目的:研究金雀异黄素对脂多糖(LPS)诱导巨噬细胞RAW264.7分泌功能的影响,探讨金雀异黄素抗炎作用及其机制,为临床应用提供理论基础。方法:用脂多糖诱导巨噬细胞株Raw264.7建立体外细胞炎症模型,金雀异黄素与巨噬细胞株Raw264.7温孵1h后,加入100ng·mL-1脂多糖以诱导巨噬细胞的活化,采用Griess试剂检测细胞培养液中NO的含量,通过双抗体夹心酶联分析法和细胞转染技术观察不同浓度金雀异黄素对脂多糖刺激巨噬细胞分泌细胞因子TNF-α、IL-6和IL-10含量及对核转录因子NF-κB和AP-1表达的影响。结果:金雀异黄素(4~111μmolL-1)能明显抑制脂多糖刺激巨噬细胞生成一氧化氮产生(P<0.05);显著抑制脂多糖诱导巨噬细胞分泌细胞因子TNF-α、IL-6和IL-10(P<0.01),并能抑制核转录因子NF-κB和AP-1的表达。结论:本实验证明了金雀异黄素有抗炎作用,其能显著降低脂多糖诱导巨噬细胞分泌NO及细胞因子TNF-α、IL-6和IL-10的释放,通过抑制这些炎症因子的释放来达到抗炎作用,金雀异黄素抑制核转录因子NF-κB和AP-1的表达来实现的。 | 崔树娜 姜俊杰 李彧 牛建昭 Ursula Bilitewski | 2010 | 中医药学报2010，38，2： | 7 |
| 7 | 不同硅铝比的Cu-ZSM-5/堇青石整体式催化剂的NO分解反应性能显示文摘考察了原位合成的不同硅铝比的Cu ZSM 5 /堇青石整体式催化剂上的NO分解反应 .结果表明 ,在 5 73~773K的温度范围内 ,硅铝比分别为 6 0 ,5 5和 40的催化剂在 72 3K下NO的转化率最高 ,而硅铝比小于 2 5的催化剂上的NO转化率很低 .低温有利于N2 O的生成 .整体式催化剂上的NO转化率低于单纯的分子筛催化剂Cu ZSM 5 。 | 单学蕾 关乃佳 曾翔 陈继新 项寿鹤 Ursula ILLGEN Manfred BAERNS | 2001 | 催化学报2001，22，3： | 6 |
| 8 | Perioperative challenges in management of diabetic patients undergoing non-cardiac surgery显示文摘Prediabetes and diabetes are important disease processes which have several perioperative implications.About one third of the United States population is considered to have prediabetes.The prevalence in surgical patients is even higher.This is due to the associated micro and macrovascular complications of diabetes that result in the need for subsequent surgical procedures.A careful preoperative evaluation of diabetic patients and patients at risk for prediabetes is essential to reduce perioperative mortality and morbidity.This preoperative evaluation involves an optimization of preoperative comorbidities.It also includes optimization of antidiabetic medication regimens,as the avoidance of unintentional hypoglycemic and hyperglycemic episodes during the perioperative period is crucial.The focus of the perioperative management is to ensure euglycemia and thus improve postoperative outcomes.Therefore,prolonged preoperative fasting should be avoided and close monitoring of blood glucose should be initiated and continued throughout surgery.This can be accomplished with either analysis in blood gas samples,venous phlebotomy or point-of-care testing.Although capillary and arterial whole blood glucose do not meet standard guidelines for glucose testing,they can still be used to guide insulin dosing in the operating room.Intraoperative glycemic control goals may vary slightly in different protocols but overall the guidelines suggest a glucose range in the operating room should be between 140 mg/dL to 180 mg/dL.When hyperglycemia is detected in the operating room,blood glucose management may be initiated with subcutaneous rapid-acting insulin,with intravenous infusion or boluses of regular insulin.Fluid and electrolyte management are other perioperative challenges.Notably diabetic ketoacidosis and hyperglycemic hyperosmolar nonketotic state are the two most serious acute metabolic complications of diabetes that must be recognized early and treated. | Ursula Galway Praveen Chahar Marc T Schmidt Jorge A Araujo-Duran Jeevan Shivakumar Alparslan Turan Kurt Ruetzler | 2021 | World Journal of Diabetes2021，12，8： | 5 |
| 9 | Methane and nitrous oxide emissions from a subtropical coastal embayment(Moreton Bay, Australia)显示文摘Surface water methane(CH4) and nitrous oxide(N2O) concentrations and fluxes were investigated in two subtropical coastal embayments(Bramble Bay and Deception Bay,which are part of the greater Moreton Bay, Australia). Measurements were done at 23 stations in seven campaigns covering different seasons during 2010–2012. Water–air fluxes were estimated using the Thin Boundary Layer approach with a combination of wind and currents-based models for the estimation of the gas transfer velocities. The two bays were strong sources of both CH4 and N2O with no significant differences in the degree of saturation of both gases between them during all measurement campaigns. Both CH4 and N2O concentrations had strong temporal but minimal spatial variability in both bays.During the seven seasons, CH4 varied between 500% and 4000% saturation while N2O varied between 128 and 255% in the two bays. Average seasonal CH4 fluxes for the two bays varied between 0.5 ± 0.2 and 6.0 ± 1.5 mg CH24/(m·day) while N2 O varied between 0.4 ± 0.1 and1.6 ± 0.6 mg N2O/(m2·day). Weighted emissions(t CO2-e) were 63%–90% N2 O dominated implying that a reduction in N2 O inputs and/or nitrogen availability in the bays may significantly reduce the bays' greenhouse gas(GHG) budget. Emissions data for tropical and subtropical systems is still scarce. This work found subtropical bays to be significant aquatic sources of both CH4 and N2O and puts the estimated fluxes into the global context with measurements done from other climatic regions. | Ronald S.Musenze Ursula Werner Alistair Grinham James Udy Zhiguo Yuan | 2015 | Journal of Environmental Sciences2015，27，3： | 5 |
| 10 | PLEIOTROPIC REGULATORY LOCUS 1 (PRL1) Integrates the Regulation of Sugar Responses with Isoprenoid Metabolism in Arabidopsis显示文摘在植物房间的 isoprenoids 的生合成从 mevalonate (MVA ) 在 cytosol 生产的先锋发生小径并且在由 methylerythritol 4 磷酸盐(MEP ) 的质体,小径,而是很少对协调两条小径的机制被知道。在 Arabidopsis thaliana 为如此的协作发信号的糖的重要性的证据被没有变化,在相关 MEP 小径抄本,蛋白质,或酶活动的层次显示出导出 MEP 的 isoprenoid 产品(叶绿素和类胡萝卜素) 的增加的累积的异种的描述这里提供。这异种被发现是新 loss-of-function 等位基因 PRL1 (多种的规章的地点 1 ) ,编码作为糖的一个全球管理者工作的保存 WD 蛋白质的基因,应力,和荷尔蒙回答,部分地由 SNF1 相关的蛋白质 kinases (SnRK1 ) 的抑制。与在 MVA 小径的主要的规章的酶的 phosphorylation 和 inactivation 的 SnRK1 kinases 的报导角色一致(hydroxymethylglutaryl 辅酶 -- reductase ) ,它的活动或蛋白质层次在 prl1 幼苗被减少。然而,导出 MVA 的结束产品(甾醇) 的累积在变异的幼苗是未改变的。蔗糖补充到野类型的幼苗 phenocopied prl1 变化以 isoprenoid 新陈代谢,建议观察 isoprenoid 显型从增加的糖累积导致 prl1 异种。在摘要, PRL1 看起来与糖,荷尔蒙,和压力回答协调 isoprenoid 新陈代谢。 | Ursula Flores-Perez Jordi Perez-Gil Marta Closa Louwrance R Wright Patricia Botella-Pavia Michael A. Phillips Albert Ferrer Jonathan Gershenzon Manuel Rodriguez-Concepcion | 2010 | Molecular Plant2010，3，1： | 5 |
| 11 | Mobilization of hematopoietic progenitor cells in patients with liver cirrhosis显示文摘AIM:To test the hypothesis that liver cirrhosis is associated with mobilization of hematopoietic progenitor cells. METHODS:Peripheral blood samples from 72 patients with liver cirrhosis of varying etiology were analyzed by flow cytometry.Identified progenitor cell subsets were immunoselected and used for functional assays in vitro. Plasma levels of stromal cell-derived factor-1(SDF-1) were measured using an enzyme linked immunosorbent assay.RESULTS:Progenitor cells with a CD133 + /CD45 + CD14 + phenotype were observed in 61%of th patients.Between 1%and 26%of the peripheral bloo mononuclear cells(MNCs)displayed this phenotype Furthermore,a distinct population of c-kit + progenito cells(between 1%and 38%of the MNCs)could b detected in 91%of the patients.Additionally,18% of the patients showed a population of progenito cells(between 1%and 68%of the MNCs)that wa characterized by expression of breast cancer resistanc protein-1.Further phenotypic analysis disclosed tha the circulating precursors expressed CXC chemokin receptor 4,the receptor for SDF-1.In line with thi finding,elevated plasma levels of SDF-1 were presen in all patients and were found to correlate with th number of mobilized CD133 + progenitor cells. | Ursula M Gehling Marc Willems Kathleen Schlagner Ralf A Benndorf Maura Dandri Jrg Petersen Martina Sterneck Joerg-Matthias Pollok Dieter K Hossfeld Xavier Rogiers | 2010 | World Journal of Gastroenterology2010，16，2： | 4 |
| 12 | One-Year Outcomes of the DA VINCI Study of VEGF Trap-Eye in Eyes with Diabetic Macular Edema显示文摘 | Diana V. Do Quan Dong Nguyen David Boyer Ursula Schmidt-Erfurth David M. Brown Robert Vitti Alyson J. Berliner Bo Gao Oliver Zeitz Rene Ruckert Thomas Schmelter Rupert Sandbrink Jeff S. Heier | 2012 | Ophthalmology2012，，8： | 4 |
| 13 | 金雀异黄素对巨噬细胞RAW 264.7增殖、形态和细胞周期的影响显示文摘目的研究金雀异黄素对巨噬细胞RAW264.7增殖、细胞形态及细胞周期的影响,以探讨其对免疫系统的作用。方法以鼠源巨噬细胞系RAW264.7为细胞模型,将金雀异黄素用二甲基亚砜(DMSO)溶解,再用培养液稀释成所需终浓度为12.5、25、50、100μmol·L-1的4组及溶剂对照组(DMSO体积含量比低于0.1%)。通过WST-1细胞增殖实验、倒置相差显微镜及流式细胞仪分别检测金雀异黄素对巨噬细胞的增殖、细胞形态及细胞周期的影响。结果金雀异黄素对巨噬细胞的影响呈时间和剂量依赖关系。50~100μmol·L-1浓度的金雀异黄素作用巨噬细胞24h和48h后均能抑制细胞增殖,细胞增殖率分别为77%~81%、58%~73%(P<0.01);并能影响细胞形态改变,表现为细胞体积增大,并形成较长伪足。流式细胞结果显示,该浓度金雀异黄素可将巨噬细胞阻滞在S-G2/M期。结论金雀异黄素能抑制巨噬细胞细胞增殖,影响巨噬细胞形态,可能与干扰细胞周期有关。 | 崔树娜 姜俊杰 艾浩 李健 Ursula Bilitewski 牛建昭 | 2010 | 北京中医药2010，29，3： | 4 |
| 14 | Modulating effects of acyl-CoA synthetase 5-derived mitochondrial Wnt2B palmitoylation on intestinal Wnt activity显示文摘AIM:To investigate the role of acyl-CoA synthetase 5(ACSL5)activity in Wnt signaling in intestinal surface epithelia.METHODS:Several cell lines were used to investigate the ACSL5-dependent expression and synthesis of Wnt2B,a mitochondrially expressed protein of the Wnt signaling family.Wnt activity was functionally assessed with a luciferase reporter assay.ACSL5-related biochemical Wnt2B modifications were investigatedwith a modified acyl-exchange assay.The findings from the cell culture models were verified using an Apcmin/+mouse model as well as normal and neoplastic diseased human intestinal tissues.RESULTS:In the presence of ACSL5,Wnt2B was unable to translocate into the nucleus and was enriched in mitochondria,which was paralleled by a significant decrease in Wnt activity.ACSL5-dependent S-palmitoylation of Wnt2B was identified as a molecular reason for mitochondrial Wnt2B accumulation.In cell culture systems,a strong relation of ACSL5 expression,Wnt2B palmitoylation,and degree of malignancy were found.Using normal mucosa,the association of ACSL5 and Wnt2B was seen,but in intestinal neoplasias the mechanism was only rudimentarily observed.CONCLUSION:ACSL5 mediates antiproliferative activities via Wnt2B palmitoylation with diminished Wnt activity.The molecular pathway is probably relevant for intestinal homeostasis,overwhelmed by other pathways in carcinogenesis. | Christina Klaus Ursula Schneider Christian Hedberg Anke K Schütz Jürgen Bernhagen Herbert Waldmann Nikolaus Gassler Elke Kaemmerer | 2014 | World Journal of Gastroenterology2014，20，40： | 4 |
| 15 | Incidents relating to the intra-hospital transfer of critically ill patients显示文摘 | Ursula Beckmann Donna M. Gillies Sean M. Berenholtz Albert W. Wu Peter Pronovost | 2004 | Intensive Care Medicine2004，，8： | 3 |
| 16 | 藏药“雪莲健骨灵”冲剂治疗成人大骨节病的效果研究显示文摘目的研究藏药'雪莲健骨灵'冲剂治疗成人大骨节病的效果。方法对324例成人大骨节病患者口服'雪莲健骨灵'冲剂6~10个疗程(3~5年),以国家'大骨节病治疗效果判定标准(Ws/T79-1996)'和国际通用的'视觉疼痛判定标准(VAS)'来观察治疗效果。结果 '雪莲健骨灵'冲剂治疗324例成人大骨节病患者6~10个疗程后,症状和体征改善有效率为60.8%~88.8%,关节功能改善有效率为44.0%~50.9%,不但患者的病情得到了很好的控制,而且止痛效果显著,关节功能恢复良好,所有治疗对象均未发生不良反应。对实验点内外环境硒含量检测,病区处于低硒水平,病区人体处于低硒营养状态;'雪莲健骨灵'冲剂硒含量很高。结论 '雪莲健骨灵'冲剂对成人大骨节病治疗效果显著,未发现毒副作用,经济实惠,是目前较好的一种药物治疗方法。其机理既有藏医药补'梅坠'的作用,亦与该药硒含量高有关。 | 索朗次仁 王治伦 Ursula Rechbach 尼玛次仁 罗布 | 2008 | 中国地方病防治2008，23，2： | 3 |
| 17 | 脾酪氨酸激酶抑制剂对脂多糖诱导巨噬细胞炎症反应的影响及机制显示文摘目的研究脾酪氨酸激酶(Syk)在LtX3诱导的Toll样受体4(TLR4)信号转导通路的作用。方法用100ng/mL脂多糖和不同浓度Syk抑制剂分别处理RAW264.7巨噬细胞后,采用Alarmar blue方法检测细胞增殖;采用Griess试剂检测细胞培养液中NO的含量;Westernblot法检测诱导型一氧化氮合成酶(iNOS)的蛋白表达;ELISA检测巨噬细胞中TNF-α和IL-6含量,RAW—Blue巨噬细胞检测核因子KB(NF—KB)和激活子蛋白-1(AP-1)的活化。结果Syk抑制剂作用于RAW264.7巨噬细胞24h,(0.5~14.0)μmol/L的Syk抑制剂对细胞增殖无明显影响,但当剂量超过1.5μmol/L时,能显著抑制LPS刺激的RAW264.7巨噬细胞NO的产生和iNOS的蛋白表达,抑制TNF-α和IL-6的产生(P〈0.01)以及NF—KB和AP-1的激活。结论Syk抑制剂降低LPS诱导的巨噬细胞分泌NO和iNOS的蛋白表达及TNF-α和IL-6的释放,通过下调Syk下游TLR4受体信号传导通路的蛋白,进而显著降低转录因子NF-KB和AP-1的激活有关。 | 崔树娜 Ursula Bilitewski | 2013 | 细胞与分子免疫学杂志2013，29，10： | 3 |
| 18 | The DA VINCI Study: Phase 2 Primary Results of VEGF Trap-Eye in Patients with Diabetic Macular Edema显示文摘 | Diana V. Do Ursula Schmidt-Erfurth Victor H. Gonzalez Carmelina M. Gordon Michael Tolentino Alyson J. Berliner Robert Vitti Rene Rückert Rupert Sandbrink David Stein Ke Yang Karola Beckmann Jeff S. Heier | 2011 | Ophthalmology2011，，9： | 3 |
| 19 | Plasma MicroRNA Profiling Reveals Loss of Endothelial MiR-126 and Other MicroRNAs in Type 2 Diabetes显示文摘 | Anna Zampetaki Stefan Kiechl Ignat Drozdov Peter Willeit Ursula Mayr Marianna Prokopi Agnes Mayr Siegfried Weger Friedrich Oberhollenzer Enzo Bonora Ajay Shah Johann Willeit Manuel Mayr | 2010 | Circulation Research2010，，6： | 3 |
| 20 | Beta-7 integrin controls enterocyte migration in the small intestine显示文摘AIM:To hypothesize that beta-7 integrin affects cellularmigration of both,lymphocytes and enterocytes.METHODS:The nucleoside analog Brd U was ip injected in beta-7-deficient mice(C57BL/6-Itgbtmlcgn/J)of male gender and age-matched male C57BL/J J mice(wild type)4,20,or 40 h before analysis.The total small intestine was isolated,dissected,and used for morphometrical studies.Brd U-positive epithelial cells were numbered in at least 15 hemi-crypts per duodenum,jejunum,and ileum of each animal.The outer most Brd U-positive cell(cellmax)was determined per hemi-crypt,numerically documented,and statistically analysed.RESULTS:Integrins containing the beta-7-chain were exclusively expressed on leukocytes.In the small intestinal mucosa of beta-7 integrin-deficient mice the number of intraepithelial lymphocytes was drastically decreased.Moreover,the Peyer’s patches of beta-7integrin-deficient mice appeared hypoplastic.In beta-7integrin-deficient mice the location of cellmax was found in a higher position than it was the case for the controls.The difference was already detected at 4 h after Brd U application,but significantly increased with time(40 h after Brd U injection)in all small intestinal segments investigated,i.e.,duodenum,jejunum,and ileum.Migration of small intestinal enterocytes was different between the experimental groups measured by cellmax locations.CONCLUSION:The E-cadherin beta-7 integrin pathway probably controls migration of enterocytes within the small intestinal surface lining epithelial layer. | Elke Kaemmerer Paula Kuhn Ursula Schneider Thomas Clahsen Min Kyung Jeon Christina Klaus Julia Andruszkow Michael Hrer Sabine Ernst Angela Schippers Norbert Wagner Nikolaus Gassler | 2015 | World Journal of Gastroenterology2015，21，6： | 3 |
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