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| 1 | CD4+ T cells and natural killer cells: Biomarkers for hepatic fibrosis in human immunodeficiency virus/hepatitis C virus-coinfected patients显示文摘AIM To characterize peripheral blood natural killer(NK) cells phenotypes by flow cytometry as potential biomarker of liver fibrosis in human immunodeficiency virus(HIV)/hepatitis C virus(HCV) coinfected patients.METHODS Peripheral mononuclear cells from 24 HIV/HCV(HBVnegative) coinfected and 5 HIV/HCV/HBV seronegative individuals were evaluated. HIV/HCV coinfected patients were divided in to groups: G1, patients with METAVIR F0-F2 and G2, patients with METAVIR F3-F4. NK surface cell staining was performed with: AntiCD3(APC/Cy7), anti-CD56(PE/Cy5), anti-CD57(APC), anti-CD25(PE), anti-CD69(FITC), anti-NKp30(PE), antiNKp46(PE/Cy7), anti-NKG2D(APC), anti-DNAM(FITC); anti-CD62L(PE/Cy7), anti-CCR7(PE), anti-TRAIL(PE), anti-Fas L(PE), anti CD94(FITC). Flow cytometry data acquisition was performed on BD FACSCanto, analyzed using Flow Jo software. Frequency of fluorescence was analyzed for all single markers. Clinical records were reviewed, and epidemiological and clinical data were obtained.RESULTS Samples from 11 patients were included in G1 and from 13 in G2. All patients were on ARV, with undetectable HIV viral load. Liver fibrosis was evaluated by transient elastography in 90% of the patients and with biopsy in 10% of the patients. Mean HCV viral load was(6.18 ± 0.7 log10). Even though, no major significant differences were observed between G1 and G2 regarding NK surface markers, it was found that patients with higher liver fibrosis presented statistically lower percentage of NK cells than individual with low to mild fibrosis and healthy controls(G2: 5.4% ± 2.3%, G1: 12.6% ± 8.2%, P = 0.002 and healthy controls 12.2% ± 2.7%, P = 0.008). It was also found that individuals with higher liver fibrosis presented lower CD4 LT count than those from G1(G2: 521 ± 312 cells/μL, G1: 770 ± 205 cells/μL; P = 0.035).CONCLUSION Higher levels of liver fibrosis were associated with lower percentage of NK cells and LTCD4+ count; and they may serve as noninvasive biomarkers of liver damage. | Natalia Laufer Diego Ojeda María Laura Polo Ana Martinez Héctor Pérez Gabriela Turk Pedro Cahn Norberto Walter Zwirner Jorge Quarleri | 2017 | World Journal of Hepatology2017，9，25： | 2 |
| 2 | Rigid fluid:animation the interplay between rigid bodies and fluid显示文摘 | Carlson M Mucha P J Turk G | 2004 | ACM Transactions on Graphics2004，23，3： | 1 |
| 3 | Micronization of pharmaceutical substances by the rapid expansion of supercritical solutions ( RESS ) : a promising method to improve the bioavailability of poorly soluble pharmaceutical agent显示文摘 | TURK M HILLS P HELFGEN B | 2002 | J Supercrit Fluids2002，22，1： | 1 |
| 4 | Image-Driven Simplification显示文摘 | Lindstrom P Turk G | 2000 | ACM Transactions on Graphics2000，19，3： | 1 |
| 5 | The effects ofpH and intraliposomal buffer strength on the rate of liposome content release and intracellular drug delivery显示文摘 | Lee R J Wang S Turk P | 1998 | BiosciRep1998，18，2： | 1 |
| 6 | Eigenfaces for recognition显示文摘 | PENTLAND A P | 1991 | Journal of Cognitive Neuroscience1991，3，1： | 1 |
| 7 | Eigenfaces for Recognition 显示文摘 | Turk M A Pentland A P | 2009 | Cognitive Neuroscience (S1001-0505)2009，3，1： | 1 |
| 8 | The trophic role of the marine cladoceran Penilia avirostris in the Gulf of Trieste显示文摘 | Lipej L Mozetic P Turk V | 1997 | Hydrobiologia1997，360，31： | 1 |
| 9 | Tumorigenic line by human polymorphonuclear leukocytes activated by lipopolysaccharide 显示文摘 | Tamatani T Turk P Weitzman S Oyasu R conversion of a rat urothelial cell | 1999 | Jpn J CancerRes1999，90，8： | 1 |
| 10 | Fast and Memory Efficient Polygonal Simplification显示文摘 | Lindstrom P Turk G | 1998 | IEEE Visualization 98 Conference Proceedings1998，，： | 1 |
| 11 | Tregs and transplantation tolerance 显示文摘 | Walsh P T Taylor D K Turks L A | 2004 | Clin Invest2004，114，10： | 1 |
| 12 | Sucrose Metabolism in Plastids 显示文摘 | Nathalie Gerrits Stefan C H J Turk Kees P M van Dun | 2001 | Plant Physiology2001，125，2： | 1 |
| 13 | Image-driven Simplification显示文摘 | Lindstrom P Turk G | 2000 | ACM Transactions on Graphics2000，19，3： | 1 |
| 14 | Optimization of melt pelletization in a high shear mixer 显示文摘 | Bukovee P Kroselj V Turk S | 2009 | Internatio- nal Journal of Pharmaceutics2009，381，2： | 1 |
| 15 | Evaluation of Memory-less Simplification显示文摘 | Lindstrom P Turk G | 1999 | IEEE Transactions on visualization and computer Graphics1999，5，2： | 1 |
| 16 | Animation of fracture by physical modeling 显示文摘 | Norton A Turk G Bacon B Gerth J Sweeney P | 1991 | The Visual Computer1991，7，4： | 1 |
| 17 | EVALUATING HIGH-RESOLUTION PRECIPITATION PRODUCTS显示文摘 | Turk F Joseph Arkin Philip Ebert Elizabeth E Sapiano Mathew R P | 2008 | Bulletin of the American Meteorological Society2008，，12： | 1 |
| 18 | Eigenfaces for Recognition显示文摘 | Turk M A Pentland A P | 2006 | Cognitive Neuroscience2006，3，1： | 1 |
| 19 | The vacuolar sorting domain of sporamin transports GUS, but not levansucrase to the plant vacuole显示文摘 | DE ROOS K SCOTTI P A | 1997 | The New Phytologist1997，136，： | 1 |
| 20 | Acute retroviral syndrome and high baseline viral load are predictors of rapid HIV progression among un- treated Argentinean seroconverters 显示文摘 | Soclas ME Sued O Laufer N Lfzaro ME Mingrone H Pryluka D Remondegui C Figueroa MI Cesar C Gun A Turk G Bouzas MB Kavasery R Krolewiecki A P6rez H Salom6u H Cahn P Grupo Argentino de Seroconversi6r Study Group | 2011 | J Int AIDS Soc2011，14，1： | 1 |
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