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| 1 | Octreotide induces caspase activation and apoptosis in human hepatoma HepG2 cells显示文摘AIM: To investigate the role of octreotide on cellular proliferation and apoptosis of human hepatoma (HepG2) cells. METHODS: We studied cellular proliferation, apoptosis and the possible internal caspase-mediated apoptosis pathway involved, after treatment of HepG2 carcinoma cells with octreotide in comparison with the apoptosis caused by tumor necrosis factor-α (TNF-α). Activities of caspase-3, caspase-9, caspase-8 and caspase-2 were studied, while apoptosis was investigated through detection of DNA fragmentation and through identification of apoptotic cells with the annexin-V/propidium iodide flow cytometric method. RESULTS: After an initial increase in HepG2 cellular proliferation, a significant inhibition was observed with 10-8 mol/L octreotide, while TNF-α dose-dependently decreased proliferation. Early and late apoptosis was significantly increased with both substances. Octreotide significantly increased caspase-3, caspase-8 and caspase-2 activity. TNF-α signifi cantly increased only caspase-2. Cellular proliferation was decreased after treatment with octreotide or TNF-α alone but, in contrast to TNF-α, octreotide decreased proliferation only at concentrations of 10-8 mol/L, while lower concentrations increased proliferation. CONCLUSION: Our findings are suggestive of caspasemediated signaling pathways of octreotide antitumor activity in HepG2 cells, and indicate that measurements of serum octreotide levels may be important, at least in clinical trials, to verify optimal therapeutic drug concentrations. | Nikos J Tsagarakis Ioannis Drygiannakis Antonis G Batistakis George Kolios Elias A Kouroumalis | 2011 | World Journal of Gastroenterology2011，17，3： | 5 |
| 2 | The impact of chronic in vivo glucocorticoid excess on the functional characteristics of human skin fibroblasts obtained from patients with endogenous Cushing's syndrome显示文摘 | Zervolea I Pratsinis H Tsagarakis S | 2005 | Eur J Endocrinol2005，152，6： | 1 |
| 3 | Endogenous subclinical hypercotisolism:diagnostic uncertainties and clinical implications显示文摘 | Vassiliadi D Thalassinos N | 2006 | J Endo Invest2006，29，： | 1 |
| 4 | The application of the combined eorticotmpin-releasing hormone plus desmopressin stimulation during petrosal sinus sampling is both sensitive and specific in differentiating patients with Cushing's disease from patients with the occult ectopie adrenoeortieotmpin syndrome显示文摘 | Tsagarakis S Vassiliadi D Kaskarelis IS | 2007 | J Clin Endocfinol Metab2007，92，： | 1 |
| 5 | Hypothalamic-pituita-ry-adrenal axis dysfunction in critically ill patients with tranmatic brain injury:incidence,pathophysiology,and relationship to vasopressor dependence and peripheral interleukin-6 levels显示文摘 | DIMOPOULOU I TSAGARAKIS S KOUYIALIS AT | 2004 | Crit Care Med2004，32，2： | 1 |
| 6 | Inversekinematics-based control of a redundantly actuated platform for rehabilitation显示文摘 | Saglia J A Tsagarakis N G Dai J S | 2009 | Proceedings of the Institution of Mechanical Engineers Part I:Journal of Systems and Control Engineering2009，223，1： | 1 |
| 7 | Development and Control of a ‘Soft-Actuated’ Exoskeleton for Use in Physiotherapy and Training显示文摘 | N.G. Tsagarakis Darwin G. Caldwell | 2003 | Autonomous Robots2003，，1： | 1 |
| 8 | Implementation conditions for energy saving technologies and practices in office buildings(Part 1):Lighting显示文摘 | Zografakis N Karyotakis K Tsagarakis K P | 2012 | Renewable and Sustainable Energy Reviews2012，12，6： | 1 |
| 9 | Endocrine abnormalities in critical care patients with moderate-to-severe head trauma:incidence,pattern and predisposing factors显示文摘 | Dimopoulou I Tsagarakis S Theodorakopoulou M | 2004 | Intensive Care Med2004，30，6： | 1 |
| 10 | TIMP-1 interaction with ctv显示文摘 | Tsagaraki I Tsilibary EC Tzinia AK | 2010 | Cell Tissue Res2010，342，1： | 1 |
| 11 | Hypothalamic-pituitary-adrenal axis dysfunction in critically ill patients with traumatic brain injury:incidence,pathophysiology,and relationship to vasopressor dependence and peripheral interleukin-6 levels显示文摘 | Dimopoulou I Tsagarakis S Kouyialis A T | 2004 | Crit Care Med2004，32，2： | 1 |
| 12 | Endogenous Subclinical hypercortisolism:diagnostic uncertainties and clinical implications显示文摘 | Tsagarakis S Vassiliadi D Thalassinos N | 2006 | J Endocrinol Invest2006，29，5： | 1 |
| 13 | Endocrine abnormalities in critical care patients with moderate-to-severe head trauma: incidence, pattern and predisposing factors 显示文摘 | Dimopoulou I Tsagarakis S Theodorakopoulou M | 2004 | Intensive Care Med2004，30，9： | 1 |
| 14 | Calcitonin - induced NF - kappaB activation up - regulates fibronectin expression in MG63 osteo- sarcoma cells 显示文摘 | Tsagaraki I Phenekos C Tsilibary E | 2013 | Anticancer Res2013，33，11： | 1 |
| 15 | Assessment of cortisol and ACTH responses to the desmopressin test in patients with Cushing’s syndrome and simple obesity显示文摘 | Tsagarakis S Vasiliou V Kokkoris P | 1999 | Clin Endocrinol (Oxf)1999，51，4： | 1 |
| 16 | Hypothalamic-pituitary-adrenal axis dysfunction in critical ly ill patients with traumatic brain injury: incidence, pathophysiology, and relationship to vasopressor dependence and peripheral interleukin-6 levels显示文摘 | DIMOPOULOU I TSAGARAKIS S KOUYIALIS A T | 2004 | Crit Care Med2004，32，2： | 1 |
| 17 | Hypothalamic-pituitary- adrenal axis dysfunction in critically ill patients with traumatic brain injury: incidence, pathophysiology and relationship to vasopressor dependence and peripheral interleukin-6 levels 显示文摘 | Dimopoulou I Tsagarakis S Kouyialis AT | 2004 | Crit Care Med2004，32，2： | 1 |
| 18 | The application of the combined corticotropin -releasing hormone plus desmoprcssin stimulation during petrosal sinus sampling is hoth sensitive and specific in differentiating patients with Cushing's disease from patients with the occult ectopic adrenocorticotropin syndrome显示文摘 | Tsagarakis S Vassiliadi D Kaskarelis IS ct al | 2007 | J Clin Endocrinol Metab2007，92，6： | 1 |
| 19 | Bio-mimetic actuators: polymeric pseudo muscular actuators and pneumatic muscle actuators for biological emulation 显示文摘 | Caldwell D G Tsagarakis N Medrano-Cerda G A | 2000 | Mechatronics2000，10，45： | 1 |
| 20 | A novel intrinsical- ly energy efficient actuator with adjustable stiffness (AwAS)显示文摘 | Jafari A Tsagarakis N G Caldwell D G | 2013 | IEEE/ASME Transactions on Mechatronics2013，18，1： | 1 |
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