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| 1 | Cranioplasty for repair of a large bone defect with autologous and homologous bone in children 显示文摘 | Brevi BC Magri AS Toma L | 2010 | J Pediatr Surg2010，45，4： | 1 |
| 2 | Beating-heart valve surgery:is the introduction of lung perfusion/ven- tilation the next step显示文摘 | Francisco IBM Edward G Tomas AS | 2011 | Future Cardiol2011，7，1： | 1 |
| 3 | Radionuclide bone imaging: an illustrative review显示文摘 | Love C Din AS Tomas MB | 2003 | Radiographics2003，23，2： | 1 |
| 4 | Cranioplasty for repair of a large bone defect with autologous and homologous bone in child ren显示文摘 | Brevi BC Magri AS Toma L | 2010 | J Pediatr Surg2010，45，4： | 1 |
| 5 | Transcriptional regulation of tocopherol biosynthesis in tomato显示文摘 | Leandro Quadrana Juliana Almeida Santiago N. Otaiza Tomas Duffy Junia V. Corrêa da Silva Fabiana Godoy Ramon Asís Luisa Bermúdez Alisdair R. Fernie Fernando Carrari Magdalena Rossi | 2013 | Plant Molecular Biology2013，，3： | 1 |
| 6 | within a Tradition- Based Vocational Training System显示文摘 | Tomas Deissinger Germany's Vocational Training Act: Its Function as an Instrument of Quality Control | 1996 | Oxford Review of Education1996，，3： | 1 |
| 7 | Cross-sectional evaluation of circulating hepatitis B virus RNA and DNA: Different quasispecies?显示文摘BACKGROUND Different forms of pregenomic and other hepatitis B virus(HBV)RNA have been detected in patients’sera.These circulating HBV-RNAs may be useful for monitoring covalently closed circular DNA activity,and predicting hepatitis B eantigen seroconversion or viral rebound after nucleos(t)ide analog cessation.Data on serum HBV-RNA quasispecies,however,is scarce.It is therefore important to develop methodologies to thoroughly analyze this quasispecies,ensuring the elimination of any residual HBV-DNA.Studying circulating HBV-RNA quasispecies may facilitate achieving functional cure of HBV infection.AIM To establish a next-generation sequencing(NGS)methodology for analyzing serum HBV-RNA and comparing it with DNA quasispecies.METHODS Thirteen untreated chronic hepatitis B patients,showing different HBV-genotypes and degrees of severity of liver disease were enrolled in the study and a serum sample with HBV-DNA>5 Log10 IU/mL and HBV-RNA>4 Log10 copies/mL was taken from each patient.HBV-RNA was treated with DNAse I to remove any residual DNA,and the region between nucleotides(nt)1255-1611 was amplified using a 3-nested polymerase chain reaction protocol,and analyzed with NGS.Variability/conservation and complexity was compared between HBV-DNA and RNA quasispecies.RESULTS No HBV-DNA contamination was detected in cDNA samples from HBV-RNA quasispecies.HBV quasispecies complexity showed heterogeneous behavior among patients.The Rare Haplotype Load at 1%was greater in DNA than in RNA quasispecies,with no statistically significant differences(P=0.1641).Regarding conservation,information content was equal in RNA and DNA quasispecies in most nt positions[218/357(61.06%)].In 102 of the remaining 139(73.38%),HBV-RNA showed slightly higher variability.Sliding window analysis identified 4 hyper-conserved sequence fragments in each quasispecies,3 of them coincided between the 2 quasispecies:nts 1258-1286,1545-1573 and 1575-1604.The 2 hyper-variable sequence fragments also coincided:nts 1311-1344 and 1461-1485.Sequences between nts 1519-1543 and 1559-1587 were only hyper-conserved in HBV-DNA and RNA,respectively.CONCLUSION Our methodology allowed analyzing HBV-RNA quasispecies complexity and conservation without interference from HBV-DNA.Thanks to this,we have been able to compare both quasispecies in the present study. | Selene Garcia-Garcia Maria Francesca Cortese David Tabernero Josep Gregori Marta Vila Beatriz Pacín Josep Quer Rosario Casillas Laura Castillo-Ribelles Roser Ferrer-Costa Ariadna Rando-Segura Jesús Trejo-Zahínos Tomas Pumarola Ernesto Casis Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2021 | World Journal of Gastroenterology2021，27，41： | 1 |
| 8 | Use of the mandibular distraction technique to treat obstructive sleep apnea syndrome显示文摘 | Brevi BC Toma L Magri AS | | 0，，： | 1 |
| 9 | Consensus Delphi study on guidelines for the assessment of anterior cruciate ligament injuries in children显示文摘Background:Knee examination guidelines in minors are intended to aid decisionmaking in the management of knee instability.Clinical question:A Delphi study was conducted with a formal consensus process using a validated methodology with sufficient scientific evidence.A group consensus meeting was held to develop recommendations and practical guidelines for use in the assessment of instability injuries in children.Key findings:there is a lack of evidence to analyse anterior cruciate ligament injuries in children and their subsequent surgical management if necessary.Diagnostic guidelines and clinical assessment of the patient based on a thorough examination of the knee are performed and a guide to anterior cruciate ligament exploration in children is developed.Clinical application:In the absence of a strong evidence base,these established guidelines are intended to assist in that decision-making process to help the clinician decide on the most optimal treatment with the aim of benefiting the patient as much as possible.Following this expert consensus,surgical treatment is advised when the patient has a subjective sensation of instability accompanied by a pivot shift test++,and may include an anterior drawer test+and a Lachman test+.If these conditions are not present,the conservative approach should be chosen,as the anatomical and functional development of children,together with a physiotherapy programme,may improve the evolution of the injury. | Angélica Campón Chekroun Jorge Velázquez-Saornil Isabel Guillén Vicente Zacarías Sánchez Milá David Rodríguez-Sanz Carlos Romero-Morales Tomas Fernandez-Jaén JoséIgnacio Garrido González MiguelÁngel Sánchez-Garrido Pedro Guillén García | 2022 | World Journal of Orthopedics2022，13，9： | 0 |
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