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| 1 | A rapid and efficient method to express target genes in mammalian cells by baculovirus显示文摘AIM: To investigate the modification of baculovirus vector and the feasibility of delivering exogenous genes into mammalian cells with the culture supernatant of Spodoptera frugiperta (Sf9) cells infected by recombinant baculoviruses. METHODS: Two recombinant baculoviruses (BacV-CMV-EGFPA, BacV-CMV-EGFPB) containing CMV-EGFP expression cassette were constructed. HepG2 cells were directly incubated with the culture supernatant of Sf9 cells infected by recombinant baculoviruses, and reporter gene transfer and expression efficiencies were analyzed by flow cytometry (FCM). The optimal transduction conditions were investigated by FCM assay in HepG2 cells. Gene-transfer and expression efficiencies in HepG2 or CV1 cells by baculovirus vectors were compared with lipofectAMINE, recombinant retrovirus and vaccinia virus expression systems. Twenty different mammalian cell lines were used to investigate the feasibility of delivering exogenous genes into different mammalian cells with the culture supernatant of infected Sf9 cells. RESULTS: CMV promoter could directly express reporter genes in Sf9 cells with a relatively low efficiency. Target cells incubated with the 1:1 diluted culture supernatant (moi=50) for 12 h at 37 ℃ could achieve the highest transduction and expression efficiencies with least impairment to cell viability. Under similar conditions the baculovirus vector could achieve the highest gene-transfer and expression efficiency than lipofectAMINE, recombinant retrovirus and vaccinia virus expression systems. Most mammalian cell lines could be transduced with recombinant baculovirus. In primate adherent culture cells the recombinant baculovirus could arrive the highest infection and expression efficiencies, but it was not very satisfactory in the cell lines from mice and suspended culture cells. CONCLUSION: Mammalian cells incubated with the culture supernatant of infected Sf9 cells could serve as a very convenient way for rapid and efficient expression of foreign genes in mammalian cells, but it might be more suitable for primate adherent culture cells. | TongCheng Chen-YuXu Ying-BinWang MinChen TingWu JunZhang Ning-ShaoXia | 2004 | World Journal of Gastroenterology2004，10，11： | 7 |
| 2 | Hydrophobicity of reactive site loop of SCCA1 affects its binding to hepatitis B virus显示文摘AIM: To investigate the role of SCCA2 and other SCCA1 molecules in the process of hepatitis B virus (HBV) binding to mammalian cells.METHODS: SCCA1 and SCCA2 were isolated from HepG2. Binding protein (BP) genes were obtained through PCR. Recombinant baculoviruses expressing SCCA1, SCCA2, BP, and different mutants were constructed and utilized to infect mammalian cells to investigate the binding ability of infected cells to HBV.RESULTS: A SCCA1 gene (A1) was isolated from HepG2, but it appeared to lack the binding ability of infected cells to HBV. Two mutants, A1-BP and BP-A1, were constructed by interchanging the carboxyl terminal of A1 and BP. Cells expressing A1-BP showed an increased virus bindingcapacity, but not BP-A1. Comparison of A1 sequence with the sequence of BP indicated the presence of only three amino acid changes in the carboxyl terminal, two of them were found in the reactive site loop (RSL) of SCCA1. Primary structure assay revealed that the hydrophobicity of BP and AJ515706 in this domain was strong, but A1 was relatively weak. Changing the aa349 of A1 from low hydrophobic glutamic acid to high hydrophobic valine enhanced HBV binding. In contrast, HBV binding was reduced by changing the aa349 of BP from valine to glutamic acid. CONCLUSION: The reslts suggest that the hydrophobicity of RSL of SCCA1 may play an important role in HBV binding to cells. | MinChen TongCheng Chen-YuXu TingWu Shan-HaiOu TaoZhang JunZhang Ning-ShaoXia | 2005 | World Journal of Gastroenterology2005，11，19： | 4 |
| 3 | Methods for measuring soil infiltration:State of the art显示文摘Soil infiltration is the process by which water on the soil surface penetrates the soil.Quantifying the soil infiltration capacity(soil infiltrability)is very important for determining components of the hydrological modeling,irrigation design and many other natural or man made processes.In this paper,commonly used methods for soil infiltration rate measurement with their principles and application conditions are introduced.The advantages and disadvantages of each method under various application conditions are discussed for comparison.Three new methods for soil infiltrability measurement,including the corresponding algorithm models,and the experimental apparatus and procedures are introduced.These analyses should facilitate the choice of method used for soil infiltrability measurement. | Mao Lili V F Bralts Pan Yinghua Liu Han Lei Tingwu | 2008 | International Journal of Agricultural and Biological Engineering2008，1，1： | 2 |
| 4 | Estimating reference evapotranspiration with the FAO Penman–Monteith equation using daily weather forecast messages显示文摘 | Jiabing Cai Yu Liu Tingwu Lei Luis Santos Pereira | 2007 | Agricultural and Forest Meteorology2007，，1： | 2 |
| 5 | Development and experimental verification of a mathematical expression for the discharge rate of a semi-circular open channel显示文摘Semi-circular open channel plays an important role in various applications and the measurement of its discharge is of interests.In this study,theoretical formulae for free overflow in a semi-circular channel are developed and presented for the discharge and wetted area relationship.The traditional discharge formulation and available experimental data are used to verify and validate the proposed relationships.The discharges calculated by using the proposed relationship show very good agreement with the experimental data sets.The results from this study supply the basis for circular weir development. | Qu Liqin Yu Xinxiao Xiao Juan Lei Tingwu | 2010 | International Journal of Agricultural and Biological Engineering2010，3，3： | 2 |
| 6 | CFD and digital particle tracking to assess flow characteristics in the labyrinth flow path of a drip irrigation emitter显示文摘 | Yunkai Li Peiling Yang Tingwu Xu Shumei Ren Xiongcai Lin Runjie Wei Hongbing Xu | 2008 | Irrigation Science2008，，5： | 2 |
| 7 | ADMETNet:The knowledge base of pharmacokinetics and toxicology network显示文摘A good drug or drug candidate should not only interact with its target molecule effectively and specifically,but also be absorbed into the body,distributed to the right location,metabolized into right compounds,and eliminated out of the body in proper manner.The processes of drug getting into and out of the body involve Absorption,Distribution,Metabolism,and Excretion(ADME),which | Quan Xu Ke Liu Xingming Lin Yangmei Qin Linshan Chen Jiao Cheng Mindong Zhong Qiushun He Yinbo Li Tingwu Wang Jianbo Pan Menglu Peng Lixia Yao Zhiliang Ji | 2017 | Journal of Genetics and Genomics2017，44，5： | 2 |
| 8 | Capacitance sensors for measuring suspended sediment concentration 显示文摘 | Li Xiaoyu Lei Tingwu Wang Wei | 2005 | Catena2005，60，3： | 1 |
| 9 | A linear source method for soil infiltrability measurement and model representations显示文摘 | Mao Lili Lei Tingwu Li Xin | 2008 | Journal of Hydrology2008，353，12： | 1 |
| 10 | Differences in right and left ventricular remodeling after transcatheter closure of atrial septal defect among adults显示文摘 | En‐TingWu TeijiAkagi ManabuTaniguchi TakeshiMaruo SatoruSakuragi ShinichiOtsuki YoshioOkamoto ShunjiSano | 2007 | Cathet. Cardiovasc. Intervent2007，，6： | 1 |
| 11 | Method for measuring velocity of shallow water flow for soil erosion with an electrolyte tracer 显示文摘 | Lei Tingwu Xia Weisheng Zhao Jun | 2005 | Journal of Hydrology2005，301，： | 1 |
| 12 | Poly (DL-lactic acid) surfaces modified by silk fibroin: ettects on the culture of osteoblast in vitro显示文摘 | Kaiyong Cai Kangde Yao Songbai Lin Zhiming Yang Xiuqiong Li Huiqi Xie Tingwu Qing and Laibao Gao | 2002 | Biomaterials2002，23，4： | 1 |
| 13 | Measuring flow velocity on frozen and non-frozen slopes of black soil through leading edge method显示文摘Flow velocity is a major parameter related to hillslope hydrodynamics erosion.This study aims to measure flow velocity over frozen and non-frozen slopes through leading edge method before being calibrated with accurate flow velocity to determine the correct coefficient for convenience of flow velocity measurement.Laboratory experiments were conducted on frozen and non-frozen soil slopes with flumes involving four slope gradients of 5°,10°,15°,and 20°and three flow rates of 1,2,and 4 L/min with a flume of 6 m long and 0.1 m wide.The measurements were made with a stopwatch to record the time duration that the water flow ran over the rill segments of 2,4 and 6 m long.Accurate flow velocity was measured with electrolyte trace method,under pulse boundary condition.The leading edge and accurate flow velocities were used to determine the correction coefficient to convert the former to the latter.Results showed that the correction coefficient on frozen soil slope was 0.81 with a coefficient of determination(R2)of 0.99.The correction coefficient on non-frozen soil slope was 0.79 with R2 of 0.98.A coefficient of 0.8 was applicable to both soil surface conditions.The accurate velocities on the four frozen black soil slopes were approximately 30%,54%,71%,and 91%higher than those on non-frozen soil slopes.By contrast,the leading edge flow velocities on the frozen soil slopes were 23%,54%,67%,and 84%higher than those on non-frozen soil slopes.The flow velocities on frozen soil slopes increased with flow rate at all four slopes,but they increased from 5 to 15°before getting stabilized.Therefore,rill flow velocity can be effectively measured with leading edge method by multiplying the leading edge velocity with a correction coefficient of 0.80.This study provides a strategy to measure rill flow velocity for studies on soil erosion mechanisms. | Chao Chen Yunyun Ban Xuefeng Wang Tingwu Lei | 2017 | International Soil and Water Conservation Research2017，5，3： | 1 |
| 14 | Effects of average velocity on the growth and surface topography of biofilmsattached on the reclaimed wastewater drip irrigation system laterals显示文摘 | Li Guibing Li Yunkai Xu Tingwu | 2012 | Irrigation Science2012，30，2： | 1 |
| 15 | A method for measuring erosive flow velocity with simulated rill显示文摘 | DONG Yuequn ZHUANG Xiaohui LEI Tingwu | 2014 | Geoder- ma2014，，232233234： | 1 |
| 16 | CFD and digital particle tracking to assess flow characteristics in the labyrinth flow path of a drip irrigation emitter显示文摘 | Li YunkaijYang Peiling Xu Tingwu | 2008 | Irrigation Science2008，26，5： | 1 |
| 17 | Micromorphology of macro- molecular superabsorbent polymer and its fractal characteristics显示文摘 | Yunkai L Tingwu X Zhiyun O | 2009 | Journal of Applied Polymer Science2009，113，6： | 1 |
| 18 | Estimating reference evapotranspiration with the FAO Penman-Monteith Equation using daily weather forecast messages显示文摘 | CAI Jiabing LIU Yu LEI Tingwu | 2007 | Agricultural and Forest Meteorology2007，145，12： | 1 |
| 19 | Estimating reference evape- transpiration with the FAO Penman-Monteith equation using daily weather forecast messages显示文摘 | Jiabing Cai Yu Liu Tingwu Lei ct al | 2007 | Agricultural and Forest Meteorology2007，，145： | 1 |
| 20 | Micromorphology of macromolecular superabsorbent polymer and its fractal characteristics 显示文摘 | Li Yunkai Xu Tingwu Ouyang Zhiyun | 2009 | Journal of Applied Polymer Science2009，113，6： | 1 |
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