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| 1 | Detection and characterization of diverse alpha-and betacoronaviruses from bats in China显示文摘Bats have been implicated as important reservoir hosts of alpha- and betacoronaviruses. In this study, diverse coronaviruses(CoVs) were detected in 50 of 951(positive rate 5.3%) intestinal specimens of eight bat species collected in four provinces and the Tibet Autonomous Region of China by pan-coronavirus RT-PCR screening. Based on 400-nt RNA-dependent RNA polymerase(Rd RP) sequence analysis, eight belonged to genus Alphacoronavirus and 42 to Betacoronavirus.Among the 50 positive specimens, thirteen gave rise to CoV full-length Rd RP gene amplification for further sequence comparison, of which three divergent sequences(two from a unreported province) were subjected to full genome sequencing. Two complete genomes of betacoronaviruses(JTMC15 and JPDB144) and one nearly-complete genome of alphacoronavirus(JTAC2) were sequenced and their genomic organization predicted. The present study has identified additional numbers of genetically diverse bat-borne coronaviruses with a wide distribution in China. Two new species of bat CoV, identified through sequence comparison and phylogenetic analysis, are proposed. | Lin Xu Fuqiang Zhang Weihong Yang Tinglei Jiang Guanjun Lu Biao He Xingyu Li Tingsong Hu Gang Chen Yun Feng Yuzhen Zhang Quanshui Fan Jiang Feng Hailin Zhang Changchun Tu | 2016 | Virologica Sinica2016，31，1： | 6 |
| 2 | Coexistence of two sympatric and morphologically similar bat species Rhinolophus affinis and Rhinolophus pearsoni显示文摘Ecologists have long focused on the coexistence of sympatric species. Here, we investigated two horseshoe bat species, Rhinolophus a nis and Rhinolophus pearsoni inhabited in the same cave, for their foraging strategies, niche di erentiation, prey selection, and their coexistence status. These two species of horseshoe bats were di erent in the dominant frequency of their echolocation calls, but similar in their morphology. We found evidence for prey selectivity although there was a high degree of overlap in prey categories and sizes. R. a nis and R. pearsoni foraged on 16 and 7 categories insects, respectively, with Pyralidae, Geometridae, Melolonthidae dominating their diets. The degree of trophic niche overlap was 0.69. Pairwise comparisons suggested that there was no obvious di erentiation in prey categories and size. However, high prey availability in the environment (Simpson diversity index = 0.79 and Margalef richness index = 4.12) contributed to their coexistence by dampening the interspeci c competition. Since there are one or more mechanisms facil- itating species coexistence in a community, our results suggest that the spatial niche di erentiation in foraging microhabitats and in for- aging habitats at landscape scale may promote the coexistence of the two bat species. However, additional eld data are needed to con rm this speculation. | Tinglei Jiang Jiang Feng Keping Sun Jing Wang | 2008 | Progress in Natural Science:Materials International2008，18，5： | 5 |
| 3 | Expression of Human Papillomavirus Type 16 L1 Protein in Transgenic Tobacco Plants显示文摘To develop a plant expression system for the production of the human papillomavirus type 16 (HPV16) vaccine, we investigated whether the HPV16 L1 protein can be expressed in tobacco plants and whether it can be used as the cheapest form of edible vaccine. The HPV16 L1 coding sequence was amplified by PCR using specific primers from the plasmid pGEM-T-HPV16 containing the template sequence, and subcloned into the intermediate vector pUCmT and binary vector pBI121 consecutively to obtain the plant expression plasmid pBI-L1. The T-DNA regions of the pBI-L1 binary vector contained the constitutive Cauliflower mosaic virus (CaMV) 35S promoter and the neomycin phosphotransferase npt Ⅱ gene, which allowed the selection of transformed plants using kanamycin. The tobacco plants were transformed by cocultivating them, using the leaf disc method, with Agrobacterium tumefaciens LBA4404, which harbored the plant expression plasmid. The regenerated transgenic tobacco plants were selected using kanamycin, and confirmed by PCR. The results of the Southern blot assay also showed that the HPV16 L1 gene was integrated stably into the genome of the transformed tobacco plants. The Western blot analysis showed that the transformed tobacco leaves could express the HPV 16 L1 protein. Furthermore, it was demonstrated by ELISA assay that the expressed protein accounted for 0.034%-0.076% of the total soluble leaf protein, was able to form 55nm virus-like particles compatible with HPV virus-like particle (VLP), and induced mouse erythrocyte hemagglutination in vitro. The present results indicate that the HPV 16 L1 protein can be expressed in transgenic tobacco plants and the expressed protein possesses the natural features of the HPV16 L1 protein, implying that the HPV16 L1 transgenic plants can be potentially used as an edible vaccine. | Hong-LiLIU Wen-ShengLI TingLEI JingZHENG ZhengZHANG Xiao-FeiYAN Zhe-ZhiWANG Yi-LiWANG Lii-ShengSI | 2005 | Acta Biochimica et Biophysica Sinica2005，37，3： | 3 |
| 4 | BURST-LDA: A NEW TOPIC MODEL FOR DETECTING BURSTY TOPICS FROM STREAM TEXT显示文摘Topic models such as Latent Dirichlet Allocation(LDA) have been successfully applied to many text mining tasks for extracting topics embedded in corpora. However, existing topic models generally cannot discover bursty topics that experience a sudden increase during a period of time. In this paper, we propose a new topic model named Burst-LDA, which simultaneously discovers topics and reveals their burstiness through explicitly modeling each topic's burst states with a first order Markov chain and using the chain to generate the topic proportion of documents in a Logistic Normal fashion. A Gibbs sampling algorithm is developed for the posterior inference of the proposed model. Experimental results on a news data set show our model can efficiently discover bursty topics, outperforming the state-of-the-art method. | Qi Xiang Huang Yu Chen Ziyan Liu Xiaoyan Tian Jing Huang Tinglei Wang Hongqi | 2014 | Journal of Electronics(China)2014，31，6： | 3 |
| 5 | Patterns and causes of geographic variation in bat echolocation pulses显示文摘Evolutionary biologists have a long-standing interest in how acoustic signals in animals vary geographically,because divergent ecology and sensory perception play an important role in speciation.Geographic comparisons are valuable in determining the factors that influence divergence of acoustic signals.Bats are social mammals and they depend mainly on echolocation pulses to locate prey,to navigate and to communicate.Mounting evidence shows that geographic variation of bat echolocation pulses is common,with a mean 5-10 kHz differences in peak frequency,and a high level of individual variation may be nested in this geographical variation.However,understanding the geographic variation of echolocation pulses in bats is very difficult,because of differences in sample and statistical analysis techniques as well as the variety of factors shaping the vocal geographic evolution.Geographic differences in echolocation pulses of bats generally lack latitudinal,longitudinal and elevational patterns,and little is known about vocal dialects.Evidence is accumulating to support the fact that geographic variation in echolocation pulses of bats may be caused by genetic drift,cultural drift,ecological selection,sexual selection and social selection.Future studies could relate geographic differences in echolocation pulses to social adaptation,vocal learning strategies and patterns of dispersal.In addition,new statistical techniques and acoustic playback experiments may help to illustrate the causes and consequences of the geographic evolution of echolocation pulse in bats. | Tinglei JIANG Hui WU Jiang FENG | 2015 | Integrative Zoology2015，10，3： | 2 |
| 6 | The crossing numbers of K1,4,n显示文摘 | Tinglei Zhao | 2006 | Discete Mathematics2006，12，： | 1 |
| 7 | Testing methods to determine properties of fiber reinforced plastic panels used for reinforcing glulams显示文摘 | Tingley D A Gai C Giltner E E | 1997 | Journal of Composites for Construction-ASCE1997，1，4： | 1 |
| 8 | Gait Analysis 显示文摘 | Chester VL Biden EN Tingley M | 2005 | Biomed Instrum Teehnol2005，39，1： | 1 |
| 9 | Gait analysis显示文摘 | Chester V L Biden E N Tingley M | 2005 | Biomedical Instrumentation and Technology2005，39，1： | 1 |
| 10 | Computer terminal work and the benefit of microbreaks显示文摘 | Mclean L Tingley M Scott RN | 2001 | Appl Ergon2001，32，： | 1 |
| 11 | Isometries of the unit sphere显示文摘 | Daryl Tingley | 1987 | Geometriae Dedicata1987，，3： | 1 |
| 12 | Development of a rapid method for compost materials显示文摘 | Mc Cartney Tingley D J | 1998 | Compost Sci Utilizat1998，6，3： | 1 |
| 13 | The crossing numbers of K1,4,n显示文摘 | Huang Yuanqiu Zhao Tinglei | 2006 | Discrete Mathematics2006，4，： | 1 |
| 14 | The unified ideal model of mean motion resonance of artificial satellites due to geopotential perturbations显示文摘From Kaula's Earth gravitational potential written in classical orbital elements, the unified ideal model of mean motion resonance of artificial satellites due to geopotential perturbations is developed in this paper first, through a suitable sequence of canonical transformations constructed by implicit functions. This unified ideal orbital resonance model is valid for all the commensurabilities between the rotational angular velocity of the Earth and the angular velocities of mean orbital motion of artificial satellites with arbitrary inclination and small eccentricity, and can be also transformed into Garfinkel's general expression of ideal resonance problem. Then 1/1 resonance of the 24-hour satellite with arbitrary inclination and small eccentricity is analyzed under the effect of harmonics of J2 and J 22 of the geopotential, based on the unified ideal model of mean motion resonance. The analytical expressions of the libration period and libration half width of the 1/1 resonance of the 24-hour satellite with arbitrary inclination and small eccentricity are presented. | ZHANG MingJiang ZHAO ChangYin XIONG YongQing SUN RongYu ZHU TingLei | 2013 | Science China(Physics,Mechanics & Astronomy)2013，56，4： | 1 |
| 15 | Effects of RG7652, a fully human mab against proprotein convertase subtilisin/kexin type 9, on LDL-C :a Phase I, randomised, double-blind, placebo- controlled, single-and multiple-dose study 显示文摘 | Tingley W Luca D Leabman M | 2013 | European Heart Journal2013，34，8： | 1 |
| 16 | Niche tracking and rapid estab- lishment of distributional equilibrium in the house sparrow show potential responsiveness of species to climate change 显示文摘 | Monahan W B Tingley M W | 2012 | PLoS ONE2012，7，42： | 1 |
| 17 | Acute passive stretch- ing alters the meehianieal properities of human plantar flexors and the optimal angle for maximal voluntary contraction 显示文摘 | Weir DE Tingley J Geoffrey Z Elder GCB | 2005 | Eur J Appl Physiol2005，93，： | 1 |
| 18 | Computer terminal work and the benefit of microbreak显示文摘 | Mclean L Tingley M Scott RN | 2004 | Appl Eryon2004，32，3： | 1 |
| 19 | Predicting the distribution of the Asian tapir in Peninsular Malaysia using maximum entropy modeling显示文摘In 2008,the IUCN threat status of the Asian tapir(Tapirus indicus)was reclasified from‘vulnerable'to‘en-dangered'.The latest distribution map from the IUCN Red List suggests that the tapirs'native range is becom-ing increasingly fragmented in Peninsular Malaysia,but distribution data collected by local researchers sug-gest a more extensive geographical range.Here,we compile a database of 1261 tapir occurrence records within Peninsular Malaysia,and demonstrate that this species,indeed,has a much broader geographical range than the IUCN range map suggests.However,extreme spatial and temporal bias in these records limits their utility for conservation planning.Therefore,we used maximum entropy(MaxEnt)modeling to elucidate the potential ex-tent of the Asian tapir's occurrence in Peninsular Malaysia while accounting for bias in existing distribution data.Our MaxEnt model predicted that the Asian tapir has a wider geographic range than our fine-scale data and the IUCN range map both suggest.Approximately 37%of Peninsular Malaysia contains potentially suit-able tapir habitats.Our results justify a revision to the Asian tapir's extent of occurrence in the IUCN Red List.Furthermore,our modeling demonstrated that selectively logged forests encompass 45%of potentially suitable tapir habitats,underscoring the importance of these habitats for the conservation of this species in Peninsular Malaysia. | Gopalasamy Reuben CLEMENTS D.Mark RAYAN Sheema Abdul AZIZ Kae KAWANISHI Carl TRAEHOLT David MAGINTAN Muhammad Fadlli Abdul YAZI Reid TINGLEY | 2012 | Integrative Zoology2012，7，4： | 1 |
| 20 | A general approach to casual mediaiton analysis显示文摘 | Imai K Keele L Tingley D | | 0，，04： | 1 |
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