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| 1 | Advance in bioequivalence assessment of topica dermatological products显示文摘Bioequivalence(BE) assessment of topical dermatological products is a long standing challenge. The development of generic topical dermatological products has often been hampered due to the limited number of acceptable approaches, which are capable of determining the BE between generic products and reference list products. The aim of this manuscript is to review different BE assessment approaches of topical dermatological products. Besides, the advances in BE evaluation and biowaivers are also provided. Currently, except in the case of dermatological corticosteroids, the golden rule to establish the BE of most topical dermatological products still heavily relied on clinical endpoint trials,which are often unreliable, time-consuming and expensive. The regulatory agencies and pharmaceutical industries are forging ahead to the development of new surrogate BE assessment approaches for other topical dermatological products. These promising approaches include dermatopharmacokinetic study(DPK), dermal microdialysis(DMD), in vitro studies, pharmacokinetic study(PK), near-infrared spectrometry(NIR), and confocal Raman spectroscopy(CRS). In addition, the expansion of biowaivers for topical dermatological products has been explored by pharmaceutical scientists. In conclusion, to accelerate the development and approval of topical dermatological products, emphasis should be put on the following areas, i.e., optimizing and standardizing the existing BE assessment methods, exploring novel alternatives of BE assessment approaches, and expanding biowaivers for topical dermatological products. | Mei Lu Haonan Xing Xiao Chen Lei Xian Jingzheng Jiang Tianzhi Yang Pingtian Ding | 2016 | Asian Journal of Pharmaceutical Sciences2016，11，6： | 4 |
| 2 | Mouse embryonic stem cells have increased capacity for replication fork restart driven by the specific Filia-Floped protein complex显示文摘Pluripotent 干细胞(PSC ) 在他们的快速的增长和作为结果的染色体不稳定性期间怀有组成的 DNA 复制应力在再生药妨碍他们的应用。理解复制的规章的机制在 PSC 强调反应因此是重要的。这里,我们报导胚胎的干细胞(转换字符) 是的那只老鼠比在解决复制应力的区分的房间优异。明确地,转换字符利用唯一的跳动 Filia 的蛋白质高效地支持阻止的复制的重启的复杂依赖者的机制分叉,因此维持 genomic 稳定性。转换字符特定的跳动 Filia 的建筑群在正常条件下面住在复制叉上。复制应力刺激他们的招募到阻止 Filia 的 151 残余是的叉和丝氨酸以一种 ATR 依赖的方式的 phosphorylated。这修正使跳动 Filia 的建筑群能充当功能的脚手架,它然后通过双机制支持拖延的叉重启:复制叉重启蛋白质, Blm,和刺激 ATR kinase 激活的两提高的招募。在 Blm 小径,脚手架招募 E3 ubiquitin ligase, Trim25,到阻止的复制叉,并且接着, Trim25 通过 ubiquitination 在阻止的复制叉拴住并且专注 Blm。在区分的房间,到复制叉和 ATR 发信号的激活的 Trim25-Blm 建筑群的招募由于转换字符特定的跳动 Filia 的支架的缺乏是更不柔韧的。因此,我们的学习表明转换字符利用另外、唯一的规章的层高效地支持阻止的叉重启并且维持 genomic 稳定性。 | Bo Zhao Weidao Zhang Yixian Cun Jingzheng Li Yan Liu Jing Gao Hongwen Zhu Hu Zhou Rugang Zhang Ping Zheng | 2018 | Cell Research2018，28，1： | 4 |
| 3 | Expression of Human Papillomavirus Type 16 L1 Protein in Transgenic Tobacco Plants显示文摘To develop a plant expression system for the production of the human papillomavirus type 16 (HPV16) vaccine, we investigated whether the HPV16 L1 protein can be expressed in tobacco plants and whether it can be used as the cheapest form of edible vaccine. The HPV16 L1 coding sequence was amplified by PCR using specific primers from the plasmid pGEM-T-HPV16 containing the template sequence, and subcloned into the intermediate vector pUCmT and binary vector pBI121 consecutively to obtain the plant expression plasmid pBI-L1. The T-DNA regions of the pBI-L1 binary vector contained the constitutive Cauliflower mosaic virus (CaMV) 35S promoter and the neomycin phosphotransferase npt Ⅱ gene, which allowed the selection of transformed plants using kanamycin. The tobacco plants were transformed by cocultivating them, using the leaf disc method, with Agrobacterium tumefaciens LBA4404, which harbored the plant expression plasmid. The regenerated transgenic tobacco plants were selected using kanamycin, and confirmed by PCR. The results of the Southern blot assay also showed that the HPV16 L1 gene was integrated stably into the genome of the transformed tobacco plants. The Western blot analysis showed that the transformed tobacco leaves could express the HPV 16 L1 protein. Furthermore, it was demonstrated by ELISA assay that the expressed protein accounted for 0.034%-0.076% of the total soluble leaf protein, was able to form 55nm virus-like particles compatible with HPV virus-like particle (VLP), and induced mouse erythrocyte hemagglutination in vitro. The present results indicate that the HPV 16 L1 protein can be expressed in transgenic tobacco plants and the expressed protein possesses the natural features of the HPV16 L1 protein, implying that the HPV16 L1 transgenic plants can be potentially used as an edible vaccine. | Hong-LiLIU Wen-ShengLI TingLEI JingZHENG ZhengZHANG Xiao-FeiYAN Zhe-ZhiWANG Yi-LiWANG Lii-ShengSI | 2005 | Acta Biochimica et Biophysica Sinica2005，37，3： | 3 |
| 4 | Energy-efficient recovery of tetrahydrofuran and ethyl acetate by triple-column extractive distillation:entrainer design and process optimization显示文摘An energy-efficient triple-column extractive distillation process is developed for recovering tetrahydrofuran and ethyl acetate from industrial effluent.The process development follows a rigorous hierarchical design procedure that involves entrainer design,thermodynamic analysis,process design and optimization,and heat integration.The computer-aided molecular design method is firstly used to find promising entrainer candidates and the best one is determined via rigorous thermodynamic analysis.Subsequently,the direct and indirect triple-column extractive distillation processes are proposed in the conceptual design step.These two extractive distillation processes are then optimized by employing an improved genetic algorithm.Finally,heat integration is performed to further reduce the process energy consumption.The results indicate that the indirect extractive distillation process with heat integration shows the highest performance in terms of the process economics. | Ao Yang Yang Su Tao Shi Jingzheng Ren Weifeng Shen Teng Zhou | 2022 | Frontiers of Chemical Science and Engineering2022，16，2： | 3 |
| 5 | 显示文摘 | SONG Jingzheng CHEN Jue TIAN Songjiu | 1999 | Journal of Pharmaceutical and Biomedical Analysis1999，21，： | 1 |
| 6 | Effect of laser offsets on joint performance of laser penetration brazing for magnesium alloy and steel 显示文摘 | Miao Yugang Han Duanfeng Yao Jingzheng | 2010 | Materials and Design2010，31，： | 1 |
| 7 | Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry显示文摘 | Li SongLin Song JingZheng Choi F F | 2009 | J Pharm Biomed Anal2009，2，49： | 1 |
| 8 | Determination of metformin in plasma by capillary electrophoresis using field-amplified sample stacking technique 显示文摘 | Song Jingzheng Chen Haifen Tian Songjiu | 1998 | J Chromntogr B1998，708，12： | 1 |
| 9 | Effect of laser offsets on joint performance of laser penetration brazing for magnesium alloy and steel显示文摘 | Yugang Miao Duanfeng Han Jingzheng Yao Feng Li | 2009 | Materials and Design2009，，6： | 1 |
| 10 | Chemical profiling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry 显示文摘 | Li Songlin Song Jingzheng Choi Franky F K | 2009 | J Pharm Biomed Anal2009，49，2： | 1 |
| 11 | A novel strategy to rapidly explore potential chemical markers for the discrimination between raw and processed Radix Rehmanniae bv UHPLC-TOF-MS with multivariate statistical analysis 显示文摘 | Li SongLin Song JingZheng Qiao ChunFeng | 2010 | J Pharm Biomed Anal2010，51，4： | 1 |
| 12 | Chemical profilingof Radix Paeoniae evaluated by ultra-performance liquid chro-matography/ photo-diode-array/quadrupole time-of-flight massspectrometry 显示文摘 | Li Songlin Song Jingzheng Choi F F | 2009 | J Pharm Biomed Anal2009，49，2： | 1 |
| 13 | Mercury in traditional medicines: Is cinnabar toxicologically similar to common mercurials 显示文摘 | Liu Jie Shi Jingzheng Yu Limei | 2008 | Exp Biol Med2008，233，7： | 1 |
| 14 | Luteolin protects against high fat diet-induced cognitive deficits in obesity mice显示文摘 | Yi Liu Xiaobin Fu Nuo Lan Sai Li Jingzheng Zhang Shuaishuai Wang Cheng Li Yanguo Shang Tonghui Huang Ling Zhang | 2014 | Behavioural Brain Research2014，，： | 1 |
| 15 | Microstruc-ture and interface characteristics of laser penetration brazed mag-nesium alloy and steel 显示文摘 | Miao Yugang Han Duanfeng Yao Jingzheng | 2010 | Science and Technology of Weldingand Joining2010，15，2： | 1 |
| 16 | Analysis of Telephone Call Detail Records based on Fuzzy Decision Tree显示文摘 | Ding Liping Gu jian Wang Yongji Wu Jingzheng | 2010 | China Communications2010，7，6： | 1 |
| 17 | Chemical profi- ling of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight spectrometry显示文摘 | Songlin Li Jingzheng Song Franky F K C | 2009 | J Pharm Biomed Anal2009，49，： | 1 |
| 18 | Security of Quantum Key Distribution (in Chinese)显示文摘 | LI Hongwei CHEN We! HUANG Jingzheng | 2012 | Scientia Sinlca Physica Mechanica & Astronomica2012，42，11： | 1 |
| 19 | 显示文摘 | Jiang Chongqiu Wang Jingzheng He Fang | 2001 | Anal Chim Acta2001，439，： | 1 |
| 20 | Chemical pro- filing of Radix Paeoniae evaluated by ultra-performance liquid chromatography/photo-diode-array/quadrupole time-of-flight mass spectrometry 显示文摘 | Songlin Li Jingzheng Song Franky F K Choi | 2009 | J Pharmaceut Biomed Anal2009，49，2： | 1 |
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