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| 1 | Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro显示文摘Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. | Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A | 2003 | Chinese Journal of Oceanology and Limnology2003，21，4： | 3 |
| 2 | Intracellular signaling and hepatocellular carcinoma显示文摘 | Polina Iakova Lubov Timchenko Nikolai A. Timchenko | 2010 | Seminars in Cancer Biology2010，，1： | 2 |
| 3 | The human melanocyte:a model system to study the complexity ofcellular aging and transformation in non fibroblastic cells 显示文摘 | Bandyopadhyay D Timchenko N Suwa T | 2001 | Exp Gerontol2001，36，8： | 1 |
| 4 | The role of CUGBP1 in age-dependent changes of liver functions 显示文摘 | Jones K Timchenko L Timchenko NA | 2012 | Ageing Res Rev2012，11，4： | 1 |
| 5 | A chamber for X-ray diffractometry of gas hydrates samples for pressures of up to 700 atm 显示文摘 | Feklistov V V Timchenko A K Ancharov A I Sheromov M A Manakov A Y | 2005 | Instruments and Experimental Techniques2005，48，6： | 1 |
| 6 | Ecological role of hydrodynamic processes in the Dnieper reservoirs显示文摘 | Sergiy Dubnyak Vladimir Timchenko | 2000 | Ecological Engineering2000，，1： | 1 |
| 7 | Role of HDAC1 in senescence,aging,and cancer显示文摘 | Willis-Martinez D Richards H W Timchenko N A | 2010 | Exp Gerontol2010，45，4： | 1 |
| 8 | Kin17,a mouse nuclear zinc finger protein that binds preferentially to curved DNA显示文摘 | Mazin A Timchenko T Ménissier-de Murcia J | 1994 | Nucleic Acids Res1994，22，20： | 1 |
| 9 | Clinical value of soluble adhesion molecules in systemic scleroderma显示文摘 | Alekperov RT Timchenko AV Aleksandrova EN | 2003 | Klin Med(Mosk)2003，81，10： | 1 |
| 10 | Prediction of hydrological phenomena using self-organizing mathematical models显示文摘 | Vasechkina E F Timchenko I E Yarin V D | 1997 | Oceanographic Literature Review1997，44，11： | 1 |
| 11 | On the solubility of hydrogen in liquid magnesium显示文摘 | SHAPOVALOV V I SEMIK A P TIMCHENKO A G | 1993 | Metall1993，，3： | 1 |
| 12 | Btcd,a mouse protein that binds to curved DNA,can substitute in Escherichia coli for H-NS,a bacterial nucleoid protein显示文摘 | Timchenko T Bailone A Devoret R | 1996 | EMBO J1996，15，15： | 1 |
| 13 | Aging and liver regeneration 显示文摘 | Timchenko NA | 2009 | Trends Endocrinol Metab2009，20，4： | 1 |
| 14 | On the solubility of hydrogen in liquid magnesium显示文摘 | SHAPOVALOV V I SEMIK A P TIMCHENKO A G | 1993 | Metallurgy1993，3，： | 1 |
| 15 | Ecological role of hydrodynamic processes in the Dnieper reservoirs显示文摘 | Dubnyak S Timchenko V | 2000 | Ecological Engineering2000，16，： | 1 |
| 16 | CCAAT/enhancer binding protein alpha regulates p21 protein and hepatocyte proliferation in newborn mice 显示文摘 | Timchenko N A Harris T E Wilde M | 1997 | Mol Cell Biol1997，17，12： | 1 |
| 17 | Effect of oral administration of tormentil mot extract(Potentilla tormentilla)on rotavirus diarrhea in children:a randomized,double blind,controlled trial显示文摘 | Subbotina Maria D Timchenko Vladimir N Vombyov Michail M | 2003 | Pediatric Infectious Disease Journal2003，22，8： | 1 |
| 18 | Effect of oral administration of tormentil root extract(Potentilla tormentilla)on rotavirus diarrhea in children:a randonized,double blind,controlled trial显示文摘 | Subbotina M D Timchenko V N Vorobyov M M | 2003 | Pediatr Infect Dis J2003，22，8： | 1 |
| 19 | Effect of oral intake of choline-stabilized orthosilicic acid on skin,nails and hair in women with photodamaged skin显示文摘 | Barel A Calomme M Timchenko A | 2005 | Arch Dermatol Res2005，297，4： | 1 |
| 20 | Aging reduces prolifera- tive capacities of liver by switching pathways of C/EBPalpha growth arrest显示文摘 | Iakova P Awad SS Timchenko NA | 2003 | Cell2003，113，4： | 1 |
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