|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Generation of Reactive Oxygen Species in Different Fractions of the Coelomocytes of Holothurian Eupentacta fraudatrix in Response to the Thermostable Toxin of Yersinia pseudotuberculosis in Vitro显示文摘Pure fraction (92%-95%) of phagocytes (FP) and a mixture of amoebocytes(62%) and morula cells (38 %) FPMC of the holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirota) were obtained by using ficoll verographine step gradient. Basal production of reactive oxygen species (ROS) in FP quantified by using reduction of nitroblue tetrazolium (NBT) was more than twice that in FPMC. Thermostable toxin of Yersinia pseudotuberculosis (TST) at different concentrations ( 0.2; 0.5; 2.5 μg/ml, but not 0.1 μg/ml) stimulated NBT reduction in FPMC after 24 h incubation. In FP, TST at concentrations of 0.1 and 0.2 μg/ml inhibited and at concentrations of 0.5 and 2.5 μg/ml stimulated NBT reduction after 24 h incubation. Maximal effect was observed in FP and FPMC at TST concentrations of 0.5 and 0.2 μg/ml, respectively. Addition of catalase (0.7 μg/ml) to the cells treated with TST (2.5 μg/ml) was followed by a decrease in NBT reduction compared to that under toxin treatment alone. TST stimulated superoxide dismutase activity in concentration dependent manner (maximum at 0.5 μg/ml concentration in FP) after 24 h treatment, and this stimulation was prevented by a commercial catalase. Plant lectin concanavalin A stimulated NBT reduction more than 5 fold in FPMC compared to the control. With addition of TST, lectin stimulated ROS to lesser extent than that with lectin alone. When catalase, TST, and lectin were added into the FPMC simultaneously, ROS increase was similar to that under lectin treatment alone. On the whole, data obtained indicated that ROS generation in holothurian coelomocytes especially occurs in both stimulated and not stimulated phagocytes, and that changes in ROS production by these cells may be one of the mechanisms of antibacterial protection of holothurians. | Dolmatova L. S. Eliseykina M. G. Timchenko N. F. Kovaleva A. L. Shitkova O. A | 2003 | Chinese Journal of Oceanology and Limnology2003，21，4： | 3 |
| 2 | A chamber for X-ray diffractometry of gas hydrates samples for pressures of up to 700 atm 显示文摘 | Feklistov V V Timchenko A K Ancharov A I Sheromov M A Manakov A Y | 2005 | Instruments and Experimental Techniques2005，48，6： | 1 |
| 3 | Role of HDAC1 in senescence,aging,and cancer显示文摘 | Willis-Martinez D Richards H W Timchenko N A | 2010 | Exp Gerontol2010，45，4： | 1 |
| 4 | Kin17,a mouse nuclear zinc finger protein that binds preferentially to curved DNA显示文摘 | Mazin A Timchenko T Ménissier-de Murcia J | 1994 | Nucleic Acids Res1994，22，20： | 1 |
| 5 | On the solubility of hydrogen in liquid magnesium显示文摘 | SHAPOVALOV V I SEMIK A P TIMCHENKO A G | 1993 | Metall1993，，3： | 1 |
| 6 | Btcd,a mouse protein that binds to curved DNA,can substitute in Escherichia coli for H-NS,a bacterial nucleoid protein显示文摘 | Timchenko T Bailone A Devoret R | 1996 | EMBO J1996，15，15： | 1 |
| 7 | On the solubility of hydrogen in liquid magnesium显示文摘 | SHAPOVALOV V I SEMIK A P TIMCHENKO A G | 1993 | Metallurgy1993，3，： | 1 |
| 8 | CCAAT/enhancer binding protein alpha regulates p21 protein and hepatocyte proliferation in newborn mice 显示文摘 | Timchenko N A Harris T E Wilde M | 1997 | Mol Cell Biol1997，17，12： | 1 |
| 9 | Effect of oral intake of choline-stabilized orthosilicic acid on skin,nails and hair in women with photodamaged skin显示文摘 | Barel A Calomme M Timchenko A | 2005 | Arch Dermatol Res2005，297，4： | 1 |
| 10 | Automated tracking and grasping of a moving object with a robotic hand-eye system显示文摘 | Allen P K Timchenko A Yoshimi B | 1993 | IEEE Trans Robot Autom1993，9，2： | 1 |
| 11 | Method of system dynamics in simulating the problems in the comprehensive use of water 显示文摘 | Fedorovskiy A D Timchenko I Ye Sirenko L A | 2004 | Hydrobiological Journal2004，，2： | 1 |
| 12 | Method of system dynamics in simulating the problems in the comprehensive use of water显示文摘 | Fedorovskiy A D Timchenko I Ye Sirenko L A | 2004 | Hydrobiological Journal2004，40，2： | 1 |
| 13 | Model for ecosystem state and water quality management in the Dnieper River Delta 显示文摘 | TIMCHENKO V OKSIYUK O GORE J A | 2000 | Ecological Engineering2000，16，1： | 1 |
| 14 | RNA CUG repeats sequester CUGBP1 and alter protein levels and activity of CUGBP1显示文摘 | Timchenko N A Cai Z J Welm A L | 2001 | Journal of Biological Chemistry2001，276，10： | 1 |
| 15 | Intracellular signaling and hepatocellular carcinoma显示文摘 | IAKOVA P TIMCHENKO L TIMCHENKO N A | 2011 | Semin Cancer Biol2011，21，1： | 1 |
| 16 | Cardiac elav-type RNA-bincling protein(ETR-3) binds to RNA CUG repeats expanded in myotonic dystrophy显示文摘 | Lu X Timchenko N A Timchenko L T | 1999 | Hum Mol Genet1999，，8： | 1 |
| 17 | Large-eddy simulation of natural convection in an asymmetri- cally-heated vertical parallel-plate channel: Assessment of subgrid-scale models 显示文摘 | LAU G E YEOH G H TIMCHENKO V REIZES J A | 2012 | Computer and Fluids2012，59，30： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员