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| 1 | Retrospective study on mixed neuroendocrine non-neuroendocrine neoplasms from five European centres显示文摘BACKGROUND Mixed neuroendocrine non-neuroendocrine neoplasm(MiNEN)is a rare diagnosis,mainly encountered in the gastro-entero-pancreatic tract.There is limited knowledge of its epidemiology,prognosis and biology,and the best management for affected patients is still to be defined.AIM To investigate clinical-pathological characteristics,treatment modalities and survival outcomes of a retrospective cohort of patients with a diagnosis of MiNEN.METHODS Consecutive patients with a histologically proven diagnosis of MiNEN were identified at 5 European centres.Patient data were retrospectively collected from medical records.Pathological samples were reviewed to ascertain compliance with the 2017 World Health Organisation definition of MiNEN.Tumour responses to systemic treatment were assessed according to the Response Evaluation Criteria in Solid Tumours 1.1.Kaplan-Meier analysis was applied to estimate survival outcomes.Associations between clinical-pathological characteristics and survival outcomes were explored using Log-rank test for equality of survivors functions(univariate)and Cox-regression analysis(multivariable).RESULTS Sixty-nine consecutive patients identified;Median age at diagnosis:64 years.Males:63.8%.Localised disease(curable):53.6%.Commonest sites of origin:colon-rectum(43.5%)and oesophagus/oesophagogastric junction(15.9%).The neuroendocrine component was;predominant in 58.6%,poorly differentiated in 86.3%,and large cell in 81.25%,of cases analysed.Most distant metastases analysed(73.4%)were occupied only by a poorly differentiated neuroendocrine component.Ninety-four percent of patients with localised disease underwent curative surgery;53%also received perioperative treatment,most often in line with protocols for adenocarcinomas from the same sites of origin.Chemotherapy was offered to most patients(68.1%)with advanced disease,and followed protocols for pure neuroendocrine carcinomas or adenocarcinomas in equal proportion.In localised cases,median recurrence free survival(RFS);14.0 months(95%CI:9.2-24.4),and median overall survival(OS):28.6 months(95%CI:18.3-41.1).On univariate analysis,receipt of perioperative treatment(vs surgery alone)did not improve RFS(P=0.375),or OS(P=0.240).In advanced cases,median progression free survival(PFS);5.6 months(95%CI:4.4-7.4),and median OS;9.0 months(95%CI:5.2-13.4).On univariate analysis,receipt of palliative active treatment(vs best supportive care)prolonged PFS and OS(both,P<0.001).CONCLUSION MiNEN is most commonly driven by a poorly differentiated neuroendocrine component,and has poor prognosis.Advances in its biological understanding are needed to identify effective treatments and improve patient outcomes. | Melissa Frizziero Xin Wang Bipasha Chakrabarty Alexa Childs Tu V Luong Thomas Walter Mohid S Khan Meleri Morgan Adam Christian Mona Elshafie Tahir Shah Annamaria Minicozzi Wasat Mansoor Tim Meyer Angela Lamarca Richard A Hubner Juan W Valle Mairéad G McNamara | 2019 | World Journal of Gastroenterology2019，25，39： | 13 |
| 2 | Methods for the extraction and RNA profiling of exosomes显示文摘AIM:To develop protocols for isolation of exosomes and characterization of their RNA content.METHODS:Exosomes were extracted from He La cell culture media and human blood serum using the Total exosome isolation(from cell culture media)reagent,and Total exosome isolation(from serum)reagent respectively.Identity and purity of the exosomes was confirmed by Nanosight?analysis,electron microscopy,and Western blots for CD63 marker.Exosomal RNA cargo was recovered with the Total exosome RNA and protein isolation kit.Finally,RNA was profiled using Bioanalyzer and quantitative reverse transcriptionpolymerase chain reaction(q RT-PCR)methodology.RESULTS:Here we describe a novel approach for robust and scalable isolation of exosomes from cell culture media and serum,with subsequent isolation and analysis of RNA residing within these vesicles.The isolation procedure is completed in a fraction of the time,compared to the current standard protocols utilizing ultracentrifugation,and allows to recover fully intact exosomes in higher yields.Exosomes were found tocontain a very diverse RNA cargo,primarily short sequences 20-200 nt(such as mi RNA and fragments of m RNA),however longer RNA species were detected as well,including full-length 18S and 28S r RNA.CONCLUSION:We have successfully developed a set of reagents and a workflow allowing fast and efficient extraction of exosomes,followed by isolation of RNA and its analysis by q RT-PCR and other techniques. | Emily Zeringer Mu Li Tim Barta Jeoffrey Schageman Ketil Winther Pedersen Axl Neurauter Susan Magdaleno Robert Setterquist Alexander V Vlassov | 2013 | World Journal of Methodology2013，3，1： | 5 |
| 3 | TRUST VIOLATION IN ELECTRONIC COMMERCE: CUSTOMER CONCERNS AND REACTIONS显示文摘 | Goles Tim Lee Simon Rao Srinivasan V Warren John | 2009 | The Journal of Computer Information Systems2009，，4： | 2 |
| 4 | Incorporating range sensing in the robot navigation function显示文摘 | Lumelsky V Tim S | 1990 | IEEE Transactions on Systems Man and Cybernetics1990，20，5： | 1 |
| 5 | Small ruminant lentivirus Tat protein induces apoptosis in caprine cells in vitro by the intrinsic pathway显示文摘 | Angela R Stphanie V Tim G | 2009 | Virology2009，383，： | 1 |
| 6 | Defining the electronic and geometric structure of one-electron oxidized copper-bis-phenoxide complexes显示文摘 | Tim S Pratik V Russell C | 2008 | J Am Chem Soc2008，130，15： | 1 |
| 7 | Identification of critical non-point pollution source areas using geographic information systems and water quality modeling显示文摘 | Tim U S Mostaghimi S Shanholtz V O | | 0，，05： | 1 |
| 8 | Detection of Closed Sharp Edges in Point Clouds Using Normal Estima- tion and Graph Theory显示文摘 | Kris D Denis V Tim V | 2007 | Computer-- Aided De- sign2007，39，4： | 1 |
| 9 | Analysis of the technical efficiency of state rubber farms in Vietnam显示文摘 | Tran V H Son Tim C Euan F | 1993 | Agricultural Economics1993，，9： | 1 |
| 10 | Implementation of a MIMO OFDM b-ased wireless LAN System 显示文摘 | ALLERT V Z TIM C | 2004 | IEEE Transaction on Signal Processing2004，52，2： | 1 |
| 11 | Identification of Critical Non-point Pollution Source Areas Using Geographic Information Systems and Water Quality Modeling显示文摘 | Tim U S Mostaghimi S Shanholtz V O | 1992 | Water Resource Bull1992，28，8： | 1 |
| 12 | A review of pressure-driven membrane processes in wastewater treatment and drinking water production 显示文摘 | Bart V D B Carlo V Tim V G | 2003 | Environmental Progress2003，22，1： | 1 |
| 13 | The surface proteins InlA and InlB are interdependently required for polar basolateral invasion by Listeria monocytogenes in a human model of the blood–cerebrospinal fluid barrier显示文摘 | Tim Gründler Natascha Quednau Carolin Stump Véronique Orian-Rousseau Hiroshi Ishikawa Hartwig Wolburg Horst Schroten Tobias Tenenbaum Christian Schwerk | 2013 | Microbes and Infection2013，，： | 1 |
| 14 | Plant sterols:Friend or foe in CNS disorders? 显示文摘 | Tim V Jeroen F J Jo M | 2015 | Progress Lipid Research2015，58，： | 1 |
| 15 | The role of networks in small and medium-sized enterprise innovation and firm performance 显示文摘 | Sarel G Martie-louise V Tim K | 2012 | Journal of Small Business Management2012，50，2： | 1 |
| 16 | The Effect of Or- ganic Materials on the Mobility and Toxicity of Met- als in Contaminated Soils显示文摘 | Rene V H Tim L Jane P | 2007 | Applied Geochemistry2007，22，： | 1 |
| 17 | Occurrence of estrogens in the Scheldt estuary: A 2-year survey 显示文摘 | Herlinde N Tim V Eric D W | 2007 | Ecotoxicology and Environmental Safety2007，66，： | 1 |
| 18 | Determination of peach firmness by analysis of impact forces显示文摘 | Michael J Delwiche Tim McDonald Stewart V Bowers | 1988 | Transactions of the ASAE1988，30，1： | 1 |
| 19 | UnidirectionalEmission of a quantum dot coupled to a nanoantenna显示文摘 | Alberto G C Giorgio V Tim H T | 2010 | Science2010，329，： | 1 |
| 20 | The moderating effect of individuals,perceptions of ethical work climate on ethical jubgments and behavioral intentions显示文摘 | Tim BT Chery V | 2000 | J Business Ethics2000，27，4： | 1 |
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