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| 1 | Comparison of lipid extraction methods for the microalgae Acutodesmus obliquus显示文摘Microalgae are widely used in the pharmaceutical and energy industries,therefore the conditions for their cultivation and extraction methods play an important role in the profiling and acquisition of lipids.The efficiency of lipid extraction from microalgae has attracted great interest from industry because of the wide variety of lipids and amounts that can be obtained.Acutodesmus obliquus(Scenedesmus obliquus UTEX 393)was used in this study.It was cultivated in Bold 3N medium modified with 75%nitrogen at 25℃,pH 6.8,125 r/min and a photoperiod of 18/6 h and illuminated with white light provided by a Light-Emitting Diode Surface Mount Device extensions(LED SMD)with an intensity of 1200μE/(m^(2)·s).The cells were stained with the Red Nile(RN)technique to indicate lipid production.Four extraction methods were compared,classical,microwave(MW),Soxhlet,and ultrasound(US),using the same solvent proportions(hexane:chloroform:methanol=1:2:3).All samples were analyzed with Fourier Transform Infrared Spectroscopy(FTIR)and Gas Chromatography coupled to Mass Spectrometry(GC-MS).The results showed:1)lipid production detected by RN was consistent with microalgal growth;2)the MW technique was the best extraction method,according to the statistical analysis through Randomized Complete Block(RCB)design and performance of 4.6%;and 3)the presence of saturated and unsaturated acids was indicated by FTIR spectra.GC-MS was able to identify palmitic and linoleic acids as the likely major constituents of the sample. | Diana Ximena Hurtado Claudia Lorena Garzón-Castro Jhon Cortés-Romero Edisson Tello Camacho | 2018 | International Journal of Agricultural and Biological Engineering2018，11，5： | 2 |
| 2 | The European Water Framework Directive and the DPSIR, a methodological approach to assess the risk of failing to achieve good ecological status显示文摘 | ángel Borja Ibon Galparsoro Oihana Solaun I?igo Muxika Eva María Tello Ainhize Uriarte Victoriano Valencia | 2005 | Estuarine Coastal and Shelf Science2005，，1： | 2 |
| 3 | Inactivating KISS1 mutation and hypogonadotropic hypogonadism 显示文摘 | Topaloglu AK Tello JA Kotan LD | 2012 | N Engl J Med2012，366，7： | 1 |
| 4 | Assessment of the thoracic aorta by spiral CT显示文摘 | Ecker CP Tello R | 1992 | AJR1992，158，4： | 1 |
| 5 | Amygdalar hyperactivity, a fear-related link between panic disorder and mesiotemporal epilepsy 显示文摘 | GEREZ M SADA A TELLO A | 2011 | Clin EEG Neurosci2011，42，： | 1 |
| 6 | Inflammation, complement, ischemia and amoebic survival in acute experimental amoebic liver abscesses in hamsters显示文摘 | A Olivos-Garc??a M Nequiz-Avenda?o E Tello R.D Mart??nez A González-Canto R López-Vancell M.C Garc??a de León I Montfort R Pérez-Tamayo | 2004 | Experimental and Molecular Pathology2004，，1： | 1 |
| 7 | In vitro sensitivity of Verticillium fungicola to selected fungicides显示文摘 | Gea F J Tello J C Honrubia M | 1996 | My copathologia1996，136，3： | 1 |
| 8 | A Novel Algorithm for Ship Detection in SAR Imagery Based on the Wavelet Transform 显示文摘 | Marivi Tello Carlos Lopez-Martinez | 2005 | IEEE GEOSCIENCE AND REMOTE SENSING LETTERS2005，2，2： | 1 |
| 9 | Ni-Mn-Ga thin films produced by pulsed laser deposition显示文摘 | TELLO P G CASTANO F J OHANDLEY R C | | Journal of Applied Physics0，91，10： | 1 |
| 10 | Perform- ance evaluation of a passive millimeter-wave imager 显示文摘 | LI Yue ARCHER J W and TELLO J | 2009 | IEEE transactions on microwave theory and techniques2009，57，10： | 1 |
| 11 | In Vivo Three-dimensional Evaluation of the Tracheobronchial Tree显示文摘 | Tello R Kruskal J Dupuy D | 1995 | J Thorac Imaging1995，10，4： | 1 |
| 12 | Developmental pattern of GFAP and vimentin gene expression in rat brain and in radial glial cultures显示文摘 | Valles S Montoliu C | 1995 | Glia1995，15，2： | 1 |
| 13 | A comparison of whole -body turboSTIR MR imaging and planar 99Tcm-methylene diphosphonate scintigraphy in the examination of patients with suspected skeletal metastases显示文摘 | Eustace S Tello R Decarvalho V | 1997 | AJR1997，169，6： | 1 |
| 14 | Transverse myelitis affect- ing more than 4 spinal segments associated with systemic lupus erythematosus: clinical, immunological, and radiological characteristics of 22 patients 显示文摘 | Espinosa G Mendizabal A Minguez S Ramo - Tello C Capellades J Olive A Cervera R | 2010 | Semin Arthritis Rheum2010，39，4： | 1 |
| 15 | C-erb-B2(HER-2/neu) protein and drug resistance in breast cancer patients treated with induction chemotherapy显示文摘 | Vargas-Roig LM Gago FE Tello O | | 0，，： | 1 |
| 16 | Inter- leukin 6 as apredictor of cardiovascular events in troponin negative nonST elevation acute coronary syndrome patients显示文摘 | Garcia Salas JM Tello Montoliu A Manzano Fern 6ndez S | 2013 | Int J Clin Pract2013，68，3： | 1 |
| 17 | A contrast agent delivery nomogram for hepatic spiral CT 显示文摘 | Tello R Seltzer SE Polger M | 1997 | J Comput Assist Tomogr1997，21，2： | 1 |
| 18 | Kisspeptin antagonists: unraveling the role of kisspeptin in reproductive physiology显示文摘 | Millar RP Roseweir AK Tello JA | 2010 | Brain Res2010，1364，： | 1 |
| 19 | Competitive Exclusion in a Two - species Chemotaxis Model 显示文摘 | Stinner C Tello J l Winkler M | 2014 | Math Boil2014，68，： | 1 |
| 20 | A novel strategy for radar imaging based on compressive sensing显示文摘 | Tello M Lopez-Dekker P and Mallorqui J J | 2010 | IEEE Transactions on Geoseience and Remote Sensing2010，48，12： | 1 |
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