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| 1 | Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing显示文摘AIM:To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells.METHODS:In this study,we have used an immortal porcine liver stem cell line,PICM-19,to study the role of c-MYC in hepatocarcinogenesis.PICM-19 cells were converted into cancer cells(PICM-19-CSCs)by overexpressing human MYC.To identify MYC-driven differential gene expression,transcriptome sequencing was carried out by RNA sequencing,and genes identified by this method were validated using real-time PCR.In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice.RESULTS:Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells(PICM-19-CSCs).By using comparative bioinformatics analyses,we have determined that>1000 genes were differentially expressed between PICM-19 and PICM-19-CSCs.Gene ontology analysis further showed that the MYCinduced,altered gene expression was primarily associated with various cellular processes,such as metabolism,cell adhesion,growth and proliferation,cell cycle,inflammation and tumorigenesis.Interestingly,six genes expressed by PICM-19 cells(CDO1,C22orf39,DKK2,ENPEP,GPX6,SRPX2)were completely silenced after MYC-induction in PICM-19-CSCs,suggesting that the absence of these genes may be critical for inducingtumorigenesis.CONCLUSION:MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver. | Rajagopal N Aravalli Neil C Talbot Clifford J Steer | 2015 | World Journal of Gastroenterology2015，21，7： | 3 |
| 2 | Uptake of Streptococcus pneumoniae by respiratory epithelial eells 显示文摘 | TALBOT U M PATON A W PATON J C | 1996 | Infeet Immun1996，64，： | 1 |
| 3 | Basic properties of sand and gravel filters 显示文摘 | Sherard J L Dunnigan L P Talbot J R | 1984 | Journal of Geotechnical Engineering ASCE1984，110，6： | 1 |
| 4 | Litter decay rates are determined by lignin chemistry显示文摘 | Talbot JM Yelle DJ Nowick J Treseder KK | 2012 | Biogeochemistry2012，108，13： | 1 |
| 5 | Analysis of Mycobacterium avium complex isolates from blood samples of AIDS patients by pulsed-field gel electrophoresis显示文摘 | Kunimoto DY Peppler MS Talbot J | | 0，，1： | 1 |
| 6 | Bad bugs need drugs, an update on the development pipeline from the Antimicrobial Availability Task Force of the Infectious Diseases Society of America显示文摘 | Talbot GH Bradley J Edwards JE | 2006 | Clin Infect Dis2006，42，5： | 1 |
| 7 | External shapes,strain rates and dyna- mics of salt structure 显示文摘 | Jackson M P A Talbot C J | 1986 | GSA Bulletin1986，97，3： | 1 |
| 8 | Bad Bugs, no drugs: no ESKAPE! an update from the infectious diseases society of America 显示文摘 | BOUCHER H W TALBOT G H BRADLEY J S | 2009 | Clinical Infectious Diseases2009，48，1： | 1 |
| 9 | A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection显示文摘 | VIAUD M C BALHDERE P V TALBOT N J | 2002 | The Plant Cell2002，14，： | 1 |
| 10 | Surface microstructure and scaling resistance of concrete显示文摘 | Talbot C Marchand J | 1996 | Cement and Concrete Research1996，26，10： | 1 |
| 11 | Differences in rat skeletal muscle after incline and dechne nmning显示文摘 | Lyrmr Talbot J A Morgan D L | 1998 | J A physiol1998，85，1： | 1 |
| 12 | Positron emission tomography with -FDG in oncology 显示文摘 | Talbot J N Petegnief Y Kerrou K | 2003 | Nuclear Instruments and Methods in Physics Research A2003，504，13： | 1 |
| 13 | The genome se- quence of the rice blast fungus Magnaporthe grisea显示文摘 | Dean R A Talbot N J Ebbole D J | 2005 | Nature2005，434，： | 1 |
| 14 | Epithelial-mesenchymal transition,the tumor microenvironment,and metastatic behavior of epithelial malignancies显示文摘 | Talbot L J Bhattacharya S D Kuo P C | | 0，，02： | 1 |
| 15 | Donation after cardiac death in the intensive care unit: the role of extracorporeal membrane oxygenation 显示文摘 | Smith J Talbot D | 2010 | Curr Anaesthesia & Critical Care2010，21，56： | 1 |
| 16 | Effects of a Peer Program on International Student Adjustment 显示文摘 | Abe J Talbot D Gellhoed R | 1998 | Journal of College Student Development1998，，6： | 1 |
| 17 | Evaluation of commercial precooiing for sweet corn 显示文摘 | Talbot M T Sargent S A Brecht J K | 1990 | Proceeding of the Florida State Horticultural society1990，102，： | 1 |
| 18 | Fractalkine mediates inflam-matory pain through activation of satellite glial cells 显示文摘 | Souza GR Talbot J Lotufo CM | 2013 | Proc NatlAcad Sci USA2013，110，11： | 1 |
| 19 | Under pressure:investigating the biology of plant infection by Magnaporthe oryzae显示文摘 | WILSON R A TALBOT N J | 2009 | Nature Reviews Microbiology2009，7，3： | 1 |
| 20 | Bad bugs, no drugs: No ESKAPE! An update from the Infectious Diseases Society of America显示文摘 | Boucher H W Talbot G H Bradley J S | 2009 | Clin lnfect Dis2009，48，1： | 1 |
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