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| 1 | Gene expression profiling of MYC-driven tumor signatures in porcine liver stem cells by transcriptome sequencing显示文摘AIM:To identify the genes induced and regulated by the MYC protein in generating tumors from liver stem cells.METHODS:In this study,we have used an immortal porcine liver stem cell line,PICM-19,to study the role of c-MYC in hepatocarcinogenesis.PICM-19 cells were converted into cancer cells(PICM-19-CSCs)by overexpressing human MYC.To identify MYC-driven differential gene expression,transcriptome sequencing was carried out by RNA sequencing,and genes identified by this method were validated using real-time PCR.In vivo tumorigenicity studies were then conducted by injecting PICM-19-CSCs into the flanks of immunodeficient mice.RESULTS:Our results showed that MYC-overexpressing PICM-19 stem cells formed tumors in immunodeficient mice demonstrating that a single oncogene was sufficient to convert them into cancer cells(PICM-19-CSCs).By using comparative bioinformatics analyses,we have determined that>1000 genes were differentially expressed between PICM-19 and PICM-19-CSCs.Gene ontology analysis further showed that the MYCinduced,altered gene expression was primarily associated with various cellular processes,such as metabolism,cell adhesion,growth and proliferation,cell cycle,inflammation and tumorigenesis.Interestingly,six genes expressed by PICM-19 cells(CDO1,C22orf39,DKK2,ENPEP,GPX6,SRPX2)were completely silenced after MYC-induction in PICM-19-CSCs,suggesting that the absence of these genes may be critical for inducingtumorigenesis.CONCLUSION:MYC-driven genes may serve as promising candidates for the development of hepatocellular carcinoma therapeutics that would not have deleterious effects on other cell types in the liver. | Rajagopal N Aravalli Neil C Talbot Clifford J Steer | 2015 | World Journal of Gastroenterology2015，21，7： | 3 |
| 2 | Uptake of Streptococcus pneumoniae by respiratory epithelial eells 显示文摘 | TALBOT U M PATON A W PATON J C | 1996 | Infeet Immun1996，64，： | 1 |
| 3 | External shapes,strain rates and dyna- mics of salt structure 显示文摘 | Jackson M P A Talbot C J | 1986 | GSA Bulletin1986，97，3： | 1 |
| 4 | A Magnaporthe grisea cyclophilin acts as a virulence determinant during plant infection显示文摘 | VIAUD M C BALHDERE P V TALBOT N J | 2002 | The Plant Cell2002，14，： | 1 |
| 5 | Surface microstructure and scaling resistance of concrete显示文摘 | Talbot C Marchand J | 1996 | Cement and Concrete Research1996，26，10： | 1 |
| 6 | Epithelial-mesenchymal transition,the tumor microenvironment,and metastatic behavior of epithelial malignancies显示文摘 | Talbot L J Bhattacharya S D Kuo P C | | 0，，02： | 1 |
| 7 | Bony Abnormalitied of the Paranasal Sinuses in Patients with Wegener's Granulomatosis显示文摘 | Yang C Talbot J M Hwang PH | 2001 | Am J Rhinol2001，15，2： | 1 |
| 8 | A maximumlikelihood approach to fitting equilibrium models of microsatellite evolution 显示文摘 | Sibly R M Whittaker J C Talbot M | 2001 | Mol Biol Evol2001，18，： | 1 |
| 9 | Autophagic fungal cell death is necessary for infection by the rice blast fungus显示文摘 | Veneault-Fourrey C Barooah M Egan M Wakley G Talbot N J | 2006 | Science2006，312，： | 1 |
| 10 | Co-feeding marine fish larvae with inert and live diets显示文摘 | Stoss J Talbot C | 1997 | Aquaculture1997，155，： | 1 |
| 11 | Pressure and velocity distribution for air flow through fruits packed in shipping containers using porous media flow analysis显示文摘 | Talbot M T Oliver C C Gaffney J J | 1990 | ASHARE Transactions1990，91，1： | 1 |
| 12 | Indenter growth in analogue models of Alphine-type deformation显示文摘 | Bonini M Sokoutis D Talbot C J | 1999 | Tectonics1999，18，1： | 1 |
| 13 | 显示文摘 | Talbot J B Electrodeposition of thin films of Nicket-Iron | 1993 | J Electrochem Soc1993，140，3： | 1 |
| 14 | Linewidth reduction in a large-smile laser diode array 显示文摘 | C L Talbot M E J Friese D Wang | 2005 | Applied Optics2005，44，29： | 1 |
| 15 | Sex differences in the LH responses to chicken LHRH- Ⅰ and Ⅱ in the domestic fowl显示文摘 | Sharp P J Dunn I C Talbot R T | 1967 | Endocrionology1967，115，： | 1 |
| 16 | Pressure and velocity distribution for air flow through fruits packed in shipping containers using porous media flow analysis显示文摘 | Talbot M T Oliver C C Gaffney J J | 1990 | ASHARE Transactions1990，91，1： | 1 |
| 17 | Electrodeposition of thin films of nickeliron 显示文摘 | GRANDE W C TALBOT J B | 1993 | Journal of the Electrochemical Society1993，140，3： | 1 |
| 18 | Fold trains in a glacier of salt in southern Iran显示文摘 | Talbot C J | 1979 | Journal of Structural Geology1979，1，1： | 1 |
| 19 | Therapeutic manipulation of the HIF hydroxylases显示文摘 | Nagel S Talbot N P Mecinovió J Smith T G Buchan A M Schofield C J | 2010 | Antioxid Redox Signal2010，12，： | 1 |
| 20 | Internal kinematics of salt diapirs显示文摘 | Talbot C J Jackson M P A | 1987 | AAPG Bulletin1987，71，9： | 1 |
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