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| 1 | Hepatogenic differentiation of mesenchymal stem cells induced by insulin like growth factor-Ⅰ显示文摘AIM:To improve hepatic differentiation of human mesenchymal stem cell(MSC)using insulin growth factor 1(IGF-Ⅰ),which has important role in liver development,hepatocyte differentiation and function.METHODS:Bone marrow of healthy donors was aspirated from the iliac crest.The adherent cells expanded rapidly and were maintained with periodic passages until a relatively homogeneous population was established.The identification of these cells was carried out by immunophenotype analysis and differentiation potential into osteocytes and adipocytes.To effectively induce hepatic differentiation,we designed a protocol based on a combination of IGF-Ⅰ and liver specificfactors(hepatocyte growth factor,oncostatin M and dexamethasone).Morphological features,hepatic functions and cytological staining were assessed to evaluate transdifferentiation of human marrow-derived MSCs.RESULTS:Flow cytometric analysis and the differentiation potential into osteoblasts and adipocytes showed that more than 90% of human MSCs which were isolated and expanded were positive by specif ic markers and functional tests.Morphological assessment and evaluation of glycogen storage,albumin and α-feto protein expression,as well as albumin and urea secretion revealed a statistically signif icant difference between the experimental groups and control.CONCLUSION:In vitro differentiated MSCs using IGF-Ⅰwere able to display advanced liver metabolic functions,supporting the possibility of developing them as potential alternatives to primary hepatocytes. | Maryam Ayatollahi Masoud Soleimani Seyed Ziaadin Tabei Maryam Kabir Salmani | 2011 | World Journal of Stem Cells2011，3，12： | 10 |
| 2 | 调节基于老鼠样品改进人的间充质的干细胞的扩大显示文摘 AIM: To improve the isolation and expansion of human marrow-derived mesenchymal stem cells (MSCs) based on rat samples. METHODS: Based on the fact that rat MSCs are relatively easy to obtain from a small aspirate, bone marrow-derived MSCs from rat were cultured and characterized to set up the different protocols used in this study. Then, accordingly, almost the same protocols were performed on human healthy bone marrow samples, after obtaining approval of the ethics committee and gaining informed consent. We used different protocols and culture conditions, including the type of basal media and the culture composition. The MSCs were characterized by immunophenotyping and differentiation. RESULTS: There was no difference in morphology and proliferation capacity between different culture media at the first passage. During the 5-7th passages, the cells gradually lost their morphology and proliferation potential on Dulbecco’s modified Eagle’s medium (DMEM) high glucose and α modified Eagle’s medium. Although the cells expanded rapidly for up to 10 passages on DMEM low glucose containing 10% to 15% fetal calf serum (FCS), their proliferation was arrested without change in morphology and differentiation capacity at the third passage on 5% FCS. Flow cytometric analysis and functional tests confirmed that more than 90% of marrow cells which were isolated and expanded by our selective protocols were MSCs. CONCLUSION: We improved the isolation and expansion of human bone marrow derived MSCs, based on rat sample experiments, for further experimental and clinical use. | Maryam Ayatollahi Maryam Kabir Salmani Bita Geramizadeh Seyed Ziaadin Tabei Masoud Soleimani Mohammad Hosein Sanati | 2012 | World Journal of Stem Cells2012，4，1： | 5 |
| 3 | Axillary lymph node dissection can be avoided in women with breast cancer with intraoperative, false-negative sentinel lymph node biopsies显示文摘 | Hiroyuki Takei Masafumi Kurosumi Takashi Yoshida Yuko Ishikawa Yuji Hayashi Jun Ninomiya Katsunori Tozuka Hanako Oba Kenichi Inoue Shigenori Nagai Yoshihiro Saito Tomoko Kazumoto Jun-ichi Saitoh Toshio Tabei | 2010 | Breast Cancer2010，，1： | 2 |
| 4 | Anti-HBc & HBV-DNA detection in blood donors negative for hepatitis B virus surface antigen in reducing risk of transfusion associated HBV infection显示文摘 | Behzad-Behbahani A Mafi-Nejad A Tabei SZ | 2006 | Indian J Med Res2006，123，1： | 1 |
| 5 | Effects of steric repulsion on helical conformation of poly ( N-propargylam- ides) with phenyl groups 显示文摘 | Deng J P Tabei J Shiotsuki M | 2004 | Macromolecules2004，37，19： | 1 |
| 6 | Bisecting GlcNAc residues on laminin-332 down-regulate galectin-3 dependent keratinocyte motility 显示文摘 | Kariya Y Kawamura C Tabei T | 2010 | J Siol Chem2010，285，5： | 1 |
| 7 | Malignant neoplasm in the axilla of a male : suspected primary carcinoma of an accessory mam- mary gland显示文摘 | Takeyama H Takahashi H Tabei I | 2010 | Breast Cancer2010，17，2： | 1 |
| 8 | Detailed analysis of rice chitinase gene expression in transgenic cucumber plants showing different levels of disease resistance to gray mold (Botrytis cinerea)显示文摘 | Kishimoto K Nishizawa Y Tabei Y | 2002 | Plant Science2002，162，5： | 1 |
| 9 | Enhancement of immunohistochemical reactivity for thymidine phosphorylase in breast carcinoma cells after administration of docetaxel as a neoadjuvant chemotherapy in advanced breast cancer patients显示文摘 | Kurosumi M Tabei T Suemasu K | 2012 | Oncol Rep2012，7，5： | 1 |
| 10 | Transgenic cucumber expressing an endogenous class III chitinase gene has reduced symptoms from Botrytis cinerea 显示文摘 | Kishimoto K Nishizawa Y Tabei Y | 2004 | Journal of General Plant Pathology2004，70，6： | 1 |
| 11 | Enhance-ment of immunohis to chemical reactivity for thymidine phosphorylase in breast carcinoma cells after adminis-tration of docetaxel as a neoadjuvant chemotherapy in advanced breast cancer patients显示文摘 | Kurosumi M Tabei T Suemasu K | 2007 | Oncol Rep2007，，5： | 1 |
| 12 | Transgeniccucumber expressing an endogenous class III chitinasegene has reduced symptoms from Botrytis cinerea显示文摘 | Kishimoto K Nishizawa Y Tabei Y | 2004 | J Gen Plant Pathol2004，70，： | 1 |
| 13 | SCARNA: fast and ac-curate structural alignment of RNA sequences by matchingfixed-length stem fragments 显示文摘 | TABEI Y TSUDA K KIN T | 2006 | Bioinformatics2006，22，14： | 1 |
| 14 | Self-assembling features of soluble nickel phthalocyanines 显示文摘 | FUJIKI M TABEI H KURIHARA T | 1988 | J Phvs Chem1988，92，5： | 1 |
| 15 | Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea)显示文摘 | Y. Tabei S. Kitade Y. Nishizawa N. Kikuchi T. Kayano T. Hibi K. Akutsu | 1998 | Plant Cell Reports1998，，3： | 1 |
| 16 | Inferring protein domains associated with drug side effects based on drug-target interaction network显示文摘 | Iwata H Mizutani S Tabei Y | 2013 | BMC Syst Biol2013，7，6： | 1 |
| 17 | Transgenic cucumber plants harboring a rice chitinase gene exhibit enhanced resistance to gray mold (Botrytis cinerea) 显示文摘 | Tabei Y Kitade S Nishizawa Y | 1998 | Plant Cell Pep1998，17，： | 1 |
| 18 | Self - assembling features of soluble nickel phthalocyanines 显示文摘 | Tabei H Kurihara T | 1988 | J Phys Chem1988，92，5： | 1 |
| 19 | Cystain C measurement and its practial use in patients with various renal diseases 显示文摘 | Tian S Kusano E Tabei K | 1997 | Clin Nephrol1997，48，2： | 1 |
| 20 | Biosafety assessment of transgenic plants in the greenhouse and the field-a case study of transgenic cucumber显示文摘 | Koga-Ban Y Tabei Y Ishimoto M | 2004 | Japan Agricultural Research Quarterly2004，3，38： | 1 |
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