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| 1 | Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma显示文摘AIM:To clarify the association of glypican-3(GPC3)expression with Wnt and other growth signaling molecules in hepatocellular carcinoma(HCC). METHODS:Expression of GPC3,Wnt,matrix metalloproteinases(MMPs),sulfatase(SULF)1,SULF2,and other growth signaling molecules was analyzed in HCC cell lines and tissue samples by real-time reverse transcriptionpolymerase chain reaction,immunoblotting,and/or immunostaining.Expression of various genes in GPC3 siRNA-transfected HCC cells was analyzed. RESULTS:GPC3 was overexpressed in most HCCs at mRNA and protein levels and its serum levels weresignificantly higher in patients with HCC than in non- HCC subjects(P<0.05).Altered expressions of various MMPs and growth signaling molecules,some of which were correlated with GPC3 expression,were observed in HCCs.Down-regulation of GPC3 expression by siRNA in GPC3-overexpressing HCC cell lines resulted in a significant decrease in expressions of MMP2,MMP14,fibroblast growth factor receptor 1,insulin-like growth factor 1 receptor.GPC3 expression was significantly correlated with nuclear/cytoplasmic localization ofβ-catenin. CONCLUSION:These results suggest that GPC3,in conjunction with MMPs and growth signaling molecules, might play an important role in the progression of HCC. | Noriyuki Akutsu Hiroyuki Yamamoto Shigeru Sasaki Hiroaki Taniguchi Yoshiaki Arimura Kohzoh Imai Yasuhisa Shinomura | 2010 | World Journal of Gastroenterology2010，16，28： | 20 |
| 2 | Transcriptional silencing of Dickkopf gene family by CpG island hypermethylation in human gastrointestinal cancer显示文摘AIM: To clarify alterations of Dickkopfs (Dkks) and Kremen2 (Krm2) in gastrointestinal cancer. METHODS: We investigated the expression profiles and epigenetic alterations of Dkks and Krm2 genes in gastrointestinal cancer using RT-PCR, tissue microarray analysis, and methylation specific PCR (MSP). Cancer cells were treated with the demethylating agent and/or histone deacetylase inhibitor. WST-8 assays and in vitro invasion assays after treatment with specific siRNA for those genes were performed. RESULTS: Dkks and Krm2 expression levels were reduced in a certain subset of the gastrointestinal cancer cell lines and cancer tissues. This was correlated with promoter hypermethylation. There were significant correlations between Dkks over-expression levels and beta-catenin over-expression in colorectal cancer. In colorectal cancers with beta-catenin over-expression, Dkk-1 expression levels were significantly lower in those with lymph node metastases than in those without. Down-regulation of Dkks expression by siRNA resulted in a significant increase in cancer cell growth and invasiveness in vitro.CONCLUSION: Down-regulation of the Dkks associated to promoter hypermethylation appears to be frequently involved in gastrointestinal tumorigenesis. | Tadateru Maehata Hiroaki Taniguchi Hiroyuki Yamamoto Katsuhiko Nosho Yasushi Adachi Nobuki Miyamoto Chie Miyamoto Noriyuki Akutsu Satoshi Yamaoka Fumio Itoh | 2008 | World Journal of Gastroenterology2008，14，17： | 19 |
| 3 | Lymphoepithelioma-like esophageal carcinoma with macroscopic reduction显示文摘Esophageal lymphoepithelioma-like carcinoma(LELC) is extremely rare. We report the first case of esopha-geal LELC showing macroscopic reduction. A 67-year-old male presented with dysphagia and, by endoscopic examination, was found to have a significantly raised tumor of 10 mm in diameter in the thoracic esophagus. The biopsied material showed esophageal cancer. We performed endoscopic submucosal dissection. However, the tumor became flattened, similar to a scar, in only 2 mo. Histologically, the carcinoma cells had infiltrated the submucosal layer. Prominent infiltration of T lymphoid cells that stained positive for CD8 was observed aroundthe carcinoma cells. Therefore, this lesion was consid-ered to be an LELC with poorly differentiated squamous cells. Because the margin was positive, an esophagec-tomy was performed. Carcinoma cells were detected in the neck in one lymph node. The staging was T1N0M1 b. However, the patient has been well, without adjuvant therapy or recurrence, for more than 5 years. | Masaya Uesato Tuguaki Kono Tooru Shiratori Yasunori Akutsu Isamu Hoshino Kentarou Murakami Daisuke Horibe Tetsurou Maruyama Yoshihide Semba Ryuma Urahama Yukiko Ogura Takashi Oide Toru Tanizawa Hisahiro Matsubara | 2014 | World Journal of Gastrointestinal Endoscopy2014，6，8： | 6 |
| 4 | 初产妇和经产妇妊娠期间的诊室和家庭血压显示文摘妊娠高血压可导致分娩时产生严重的并发症,如脑卒中、早产或低出生体质量。初产妇被认为是妊娠高血压的一个危险因素。产次与妊娠期间家庭血压相关性仍不明确。方法:入选初产妇和经产妇共575名,评估妊娠高血压和先兆子痫的发病率。收集在一家日本产科医院接受产前检查的530名正常血压孕妇的家庭和诊室血压值。诊室血压为产前检查2次测量的血压平均值,参与者妊娠期间每日清晨在家自测血压。 | Ishikuro M Obara T Metoki H Ohkubo T Yamamoto M Akutsu K Sakurai K Iwama N Katagiri M Yagihashi K Yaegashi N Mori S Suzuki M Kuriyama S Imai Y 罗冬梅 叶鹏 | 2013 | 中华高血压杂志2013，21，6： | 2 |
| 5 | A rapid bedside D-dimer as- say( cardiac D-dimer) for screening of clinically suspected acute aor- tic dissection显示文摘 | Akutsu K Sato N Yamamoto T | 2005 | Circ J2005，69，4： | 1 |
| 6 | Epigenetic instability in ES cells and cloned mice显示文摘 | Humpherys D Eggan K Akutsu H Hochedlinger K Rideout WM 3rd Biniszkiewicz D | 2001 | Science2001，293，5527： | 1 |
| 7 | Cloned mice have an obese phenotype not transmitted to their offspring显示文摘 | Tamashiro K L Wakayama T Akutsu H | 2002 | Nat Med2002，8，3： | 1 |
| 8 | Effects of the patient false lumen on the long-term outcome of type B acute aortic dissection 显示文摘 | Akutsu K Nejima J Kiuchi K | 2004 | Eur J Cardiothorac Surg2004，26，2： | 1 |
| 9 | In vitro cytotoxic effects of fludarabine (2-F-ara-A) in combination with commonly used anti-leukemic agents by isobologram analysis显示文摘 | Kano Y Akutsu M Tsunoda S | 2000 | Leukemia2000，14，3： | 1 |
| 10 | Exerciseinduced ST-segment depression: imbalance between myocardial oxygen demand and myocardial blood flow显示文摘 | Watanabe T Akutsu Y Yamanaka H | 2000 | Acta Cardiol2000，55，： | 1 |
| 11 | Determination of Acetamiprid, Imidacloprid, and Nitenpyram Residues in Vegetables and Fruits by High-performance Liquid Chromatography with Diode-army Detection显示文摘 | OBANA H OKIHASHI M AKUTSU K | 2002 | Agric Food Chem2002，50，16： | 1 |
| 12 | A phase I / Il study of second-line chemotherapy with fractionated docetaxel and nedaplatin for 5-FUlcisplatin-resistant esophageal squamous cell carcinoma 显示文摘 | Akutsu Y Shuto K Kono T | 2012 | Hepatogastroenterology2012，59，119： | 1 |
| 13 | Development of high strength spring steel显示文摘 | Akutsu T Tange A Satoh Y | 1992 | Electric Furnace Steel1992，63，1： | 1 |
| 14 | Effects of alphapinene odor in different concentrations on stress-induced hyperthermia in rats显示文摘 | Akutsu I-I Kikusui T Takeuchi Y | 2003 | Jour of Verer Medic Scien2003，65，9： | 1 |
| 15 | Importance of balance between extracellular matrix synthesis and degradation in basement memhrane form ation显示文摘 | Amano S Akutsu N Matsunaga Y | 2001 | Exp Cell Res2001，271，： | 1 |
| 16 | X-Chromosome inactivation in cloned mouse embryos 显示文摘 | Eggan K Akutsu H Hochedlinger K | 2000 | Science2000，290，5496： | 1 |
| 17 | Role of histonedeacetylase inhibitor in adenovirus- mediated p53 gene therapy in esophagealcancer显示文摘 | HOSHINO I MATSUBARA H AKUTSU Y | 2008 | Anticancer Res2008，28，： | 1 |
| 18 | Combined laminoplasty with posterior lateral mass plate for unstable spondylotic cervical canal stenosis-technical note显示文摘 | Matsumura A Yanaka K Akutsu H | | 0，，10： | 1 |
| 19 | Importance of balance between extracellular matrix synthesis and degradation in basement membrane formation显示文摘 | Amano S Akutsu N Matsunaga Y | 2001 | Exp Cell Res2001，271，： | 1 |
| 20 | Epigenetic instability in ES cells and cloned mice显示文摘 | Humpherys D Eggan K Akutsu H | 2001 | Science2001，293，： | 1 |
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