|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | 手术期间血糖控制对糖尿病视网膜病变和黄斑病变的影响显示文摘目的:评估手术期间血糖控制对糖尿病视网膜病变和黄斑病变进展的影响。 | Chikako Suto Sadao Hori SatoshI Kato Kanemitsu Muraoka Shigehiko Kitano 刘亮(译) | 2006 | 美国医学会眼科杂志(中文版)2006，18，4： | 16 |
| 2 | A robust homogeneous binding assay for α4β2 nicotinic acetylcholine receptor显示文摘<正> Aim: To develop a homogeneous high-throughput screening (HTS) assay based on scintillation proximity assay (SPA) technology for identification of novel α4β2 nicotinic acetylcholine receptor (nAChR) modulators. Methods: Membrane preparation of HEK293 cells expressing α4β2 nAChR, [3~H]cytisine and wheat germ agglutinin (WGA)-coupled microbeads were used to develop an HTS assay based on SPA technology. This method was validated against a conventional filter binding approach and applied to large-scale screening of a library containing 32000 synthetic compounds. Intracellular calcium measurement was carried out to verify the bioactivities of the hits found by the SPA assay. Results: IC_(50) values of 2 reference compounds (epibatidine and RJR 2403) determined by SPA and filter binding methods were comparable and consistent with those reported elsewhere. A total of 54 compounds, showing more than 60% competitive inhibition on [3~H]cytisine binding to α4β2 nAChR, were identified initially following an HTS campaign. Secondary screening confirmed that 17 compounds with novel chemical structures possessed relatively high binding affinity to α4β2 nAChR (K_i<2 μmol/L). Eight compounds displayed antagonistic effects with>50% inhibition on ABT-594-induced calcium mobilization while none showed any agonistactivity. Conclusions: This homogeneous binding assay is a highly efficient, amenable to automation and robust tool to screen potential α4β2 nAChR modulators in an HTS setting. Its application may be expanded to other membrane receptors and ion channels. | Xin HUI Jie GAO Xin XIE Naoki SUTO Tsuyoshi OGIKU Ming-Wei WANG | 2005 | Acta Pharmacologica Sinica2005，26，10： | 4 |
| 3 | A mechanism for the specific immunogenicity of heat shock protein-chaperoned peptides显示文摘 | Suto R Srivastava PK | | 0，，： | 3 |
| 4 | High quality machining of ceramics 显示文摘 | Tsutsumi C Okano K Suto T | 1993 | Journal of Materials Processing Technology1993，37，： | 2 |
| 5 | Hemoperfusion with polymyxin B-immobilized fiber column improves liver function after ischemia-reperfusion injury显示文摘AIM:To investigate the usefulness of direct hemoperfusion with a polymyxin B-immobilized fiber column (DHP-PMX therapy)for warm hepatic ischemia-reperfusion(I/R)injury after total hepatic vascular exclusion (THVE)using a porcine model. METHODS:Eleven Mexican hairless pigs weighing 22-38 kg were subjected to THVE for 120 min and then observed for 360 min.The animals were divided into two groups randomly:the DHP-PMX group(n=5)underwent DHP-PMX at a flow rate of 80 mL/min for 120 min(beginning 10 min before reperfusion),while the control group did not(n=6).The rate pressure product (RPP):heart rate×end-systolic arterial blood pressure, hepatic tissue blood flow(HTBF),portal vein blood flow (PVBF),and serum aspartate aminotransferase(AST) levels were compared between the two groups. RESULTS:RPP and HTBF were significantly(P< 0.05)higher in the DHP-PMX group than in the control group 240 and 360 min after reperfusion.PVBF in the DHP-PMX group was maintained at about 70%of the flow before ischemia and differed significantly(P <0.05)compared to the control group 360 min after reperfusion.The serum AST increased gradually afterreperfusion in both groups,but the AST was significantly(P<0.05)lower in the DHP-PMX group 360 min after reperfusion. CONCLUSION:DHP-PMX therapy reduced the hepatic warm I/R injury caused by THVE in a porcine model. | Hiroaki Sato Kiyohiro Oshima Katsumi Kobayashi Hodaka Yamazaki Yujin Suto Izumi Takeyoshi | 2009 | World Journal of Gastroenterology2009，15，36： | 2 |
| 6 | Direct hemoperfusion with a polymyxin B-immobilized cartridge in intestinal warm ischemia reperfusion显示文摘AIM: To investigate the effectiveness of direct hemo- perfusion with polymyxin B-immobilized fibers (DHP- PMX therapy) on warm ischemia-reperfusion (I/R) injury of the small intestine. METHODS: The proximal jejunum and distal ileum of mongrel dogs were resected. Warm ischemia was performed by clamping the superior mesenteric artery (SMA) and vein (SMV) for 2 h. Blood flow to the proximal small intestine was restored 1 h after reperfusion, and the distal small intestine was used as a stoma. The experiment was discontinued 6 h after reperfusion. The dogs were divided into two groups: the DHP-PMX group (n = 6, DHP-PMX was performed for 180 min; from 10 min prior to reperfusion to 170 min after reperfusion) and the control group (n = 5). The rate pressure product (RPP), SMA blood flow, mucosal tissue blood flow, and intramucosal pH (pHi) were compared between the two groups. The serum interleukin (IL)-10 levels measured 170 min after reperfusion were also compared. RESULTS: The RPP at 6 h after reperfusion was significantly higher in the PMX group than in the control group (12 174 ± 1832 mmHg/min vs 8929 ± 1797 mmHg/min, P < 0.05). The recovery rates ofthe SMA blood flow at 1 and 6 h after reperfusion were significantly better in the PMX group than in the control group (61% ± 7% vs 44% ± 4%, P < 0.05, and 59% ± 5% vs 35% ± 5%, P < 0.05, respectively). The recovery rate of the mucosal tissue blood flow and the pHi levels at 6 h after reperfusion were significantly higher in the PMX group (61% ± 8% vs 31% ± 3%, P < 0.05 and 7.91 ± 0.06 vs 7.69 ± 0.08, P < 0.05, respectively). In addition, the serum IL-10 levels just before DHP-PMX removal were significantly higher in the PMX group than in the control group (1 569 ± 253 pg/mL vs 211 ± 40 pg/mL, P < 0.05). CONCLUSION: DHP-PMX therapy reduced warm I/R injury of the small intestine. IL-10 may play a role in inhibiting I/R injury during DHP-PMX therapy. | Hiroaki Sato Kazuhisa Arakawa Katsumi Kobayashi Hodaka Yamazaki Yujin Suto Izumi Takeyoshi Kiyohiro Oshima | 2008 | World Journal of Gastroenterology2008，14，35： | 2 |
| 7 | A mechanism for the specific immunogenicity of heat shock protein-chaperuned peptides显示文摘 | Suto R Srivastava P K | 1995 | Science1995，269，5230： | 1 |
| 8 | CO2 injection to granite and sandstone in experimental rock/hot water systems显示文摘 | Liu L H Suto Y Bignall G | 2003 | Energy Conversion and Management2003，44，： | 1 |
| 9 | Conversion of D- arabinose to D-erythroascorbic acid and oxalic acid in Sclerotinia sclerotiorum 显示文摘 | Loewus FA Saito K Suto RK | 1995 | Biochem Biophys Res Commun1995，212，： | 1 |
| 10 | Pentacyclic laddersiloxane 显示文摘 | Unno M Suto A Matsumoto H | 2002 | J Am Chem Soc2002，124，8： | 1 |
| 11 | Neurovirulence of H7N7 influenza A virus: brain stem encephalitis accompanied with aspiration pneumonia in mice 显示文摘 | Shinya K Suto A Kawakami M | 2005 | Arch Virol2005，150，8： | 1 |
| 12 | Stigma Shrinks my Bubble:a qualitative study of understandings and experiences of stigma and bipolar disorder显示文摘 | Suto M Livingston JD Hole R | 2012 | Stigma Res Action2012，2，2： | 1 |
| 13 | Tenascin-C is upregulated in the skin lesions of patients with atopic dermatitis显示文摘 | Kaoru Ogawa Mikito Ito Kaori Takeuchi Akiko Nakada Masayuki Heishi Hajime Suto Kouichi Mitsuishi Yuji Sugita Hideoki Ogawa Chisei Ra | 2005 | Journal of Dermatological Science2005，，： | 1 |
| 14 | HMG-CoA reductase inhibitor simvastatin inhibits proinflammatory cytokine production from murine mast cells 显示文摘 | Shin-ichiro Kagami Hiroko Kanari Akira Suto | 2008 | Int Arch Allergy Immunol2008，146，1： | 1 |
| 15 | Necrotic but not apoptotic cell death release heat shock proteins, which deliver a partial maturation signal to dendritic cells and activate the NFκB pathway显示文摘 | Binder RJ Suto R | 2000 | Int Im munol2000，12，： | 1 |
| 16 | Necrotic but not apoptotic cell death releases heat shock proteins,which deliver a partial maturation signal to dendritic cells and activate the NF kappa Bpathway显示文摘 | Basu S Binder R J Suto R | 2000 | Int Immunol2000，12，： | 1 |
| 17 | Optical coherence tomographyat the neurosensory retina in rhegmatogenous retinal detachment显示文摘 | Haugimura N Suto K Iida T | 2000 | Am J Ophthalmol2000，129，2： | 1 |
| 18 | Plexin-A4 mediates 5Axon-repulsive activities of both secreted and transmembrane semaphorins and plays roles in nerve fiber guidance显示文摘 | Fumikazu Suto Keisuke Ito | 2005 | J Neurosci2005，25，14： | 1 |
| 19 | Efficacy of the tyrosine kinase inhibitor gefitinib in a patient with metastatic small cell lung cancer显示文摘 | Araki J Okamoto R Suto Y | 2005 | Lung Cancer2005，48，1： | 1 |
| 20 | Induction and catabolite repression mechanisms ofcellulase in fungi显示文摘 | Suto M and Tomita F | 2001 | Joumal of Bioscience and Bioengineering2001，92，4： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员