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| 1 | 2种分子标记应用于澄黄滨珊瑚群体遗传多样性研究中的可行性显示文摘利用PCR技术对澄黄滨珊瑚的2种分子标记(线粒体细胞色素氧化酶亚基1基因COI和核糖体RNA内转录间隔区基因ITS)进行测序,探讨COI序列和ITS序列在澄黄滨珊瑚(Porites lutea)群体研究中的适用性,并从中选出最适合研究澄黄滨珊瑚群体遗传多样性的分子标记.结果显示COI序列变异位点少,成对序列差异在群体内、群体间都很小,不适于澄黄滨珊瑚的群体研究;而ITS区序列个体内成对的序列差异仅为0.35%,可以作为研究澄黄滨珊瑚群体遗传多样性的分子标记;通过对ITS1、ITS2、5.8S rRNA、ITS1+ITS2及ITS区序列的比较,认为ITS1+ITS2序列是最适合研究澄黄滨珊瑚群体遗传多样性的分子标记.本研究为以后研究我国沿岸造礁石珊瑚澄黄滨珊瑚的群体遗传结构提供方法依据,从而为保护管理和恢复受损珊瑚礁生态系统提供遗传学数据支持. | 田鹏 牛文涛 林荣澄 陈彬 Suharsono | 2014 | 应用海洋学学报2014，33，1： | 2 |
| 2 | Male-sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat显示文摘 | MICHEL HERNOULD SONY SUHARSONO SIMON LITVAK | 1993 | Plant Biology1993，90，： | 1 |
| 3 | Male-sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat显示文摘 | Hernould M Suharsono S Litvak S | 1993 | Proc Natl Acad Sel1993，90，6： | 1 |
| 4 | Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants显示文摘 | Michel Hernould S. Suharsono Eduardo Zabaleta Jean Pierre Carde Simon Litvak Alejandro Araya Armand Mouras | 1998 | Plant Molecular Biology1998，，4： | 1 |
| 5 | The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in: disease resistance of rice显示文摘 | SUHARSONO U FUJISAWA Y KAWASAKI T | 2002 | Proc Natl Acad Sci USA2002，99，13： | 1 |
| 6 | Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants显示文摘 | Hernould M Suharsono Zabaleta E Carde J P Litvak S Araya A Mouras A | 1998 | Plant Mol Biol1998，36，： | 1 |
| 7 | Impairment of tapetum and mitochondria in engineered male - sterile tobacco plants显示文摘 | Hernould M Suharsono Zabaleta E | 1998 | Plant Mol Bio1998，36，： | 1 |
| 8 | The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice 显示文摘 | Suharsono U Fujisawa Y Kawasaki T | 2002 | Proceedings of the National Academy of Sciences of the United States of America2002，99，13： | 1 |
| 9 | Malesterility induction in transgenic tobacco plants with an unedited atp6 mitochondrial gene from wheat 显示文摘 | Hernould M Suharsono S Litvak S Araya A Mouras A | 1993 | Proc Natl Acad Sci USA1993，90，6： | 1 |
| 10 | Impairment of tapetum and mitochondria in engineered male-sterile tobacco plants显示文摘 | Michel Hernould S. Suharsono Eduardo Zabaleta Jean Pierre Carde Simon Litvak Alejandro Araya Armand Mouras | 1998 | Plant Molecular Biology1998，，4： | 1 |
| 11 | Male-sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat显示文摘 | Hernould M Suharsono S Litvaks | 1993 | Pro Natl Acad Sei1993，90，： | 1 |
| 12 | Male - sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat显示文摘 | Hernould M Suharsono S Litvak S | 1993 | Proc Natl Acad Sci USA1993，90，： | 1 |
| 13 | Impairment of tapetum and mitochondria in engineered male-sterile tobacco plant显示文摘 | Hernould M Suharsono Zabaleta E Carde J P Litvak S Araya A Mouras A | 1998 | Plant Mol Biol1998，36，： | 1 |
| 14 | Male sterility induction in transgenic tobacco plant with an unedited atp9 mitochondrial gene from wheat显示文摘 | Hernould M Suharsono S Litvak S Araya A Mouras A | 1993 | Proc Natl Acad Sci USA1993，90，6： | 1 |
| 15 | Impairment of tapetum and mitochondria in ergineered male-sterile tobacco plant显示文摘 | Hernould M Suharsono S Zebaleta E Carde P J Litvak S | 1998 | Plant Mol Biol1998，36，4： | 1 |
| 16 | 蓝碧海峡澄黄滨珊瑚群体遗传多样性显示文摘通过对印度尼西亚蓝碧海峡3个澄黄滨珊瑚(Porites lutea)地理群体(YM、YN、YS)的79个样本核糖体RNA转录单元内间隔区ITS基因(包括ITS1区和ITS2区)片段进行克隆、测序,探讨澄黄滨珊瑚的群体遗传多样性和遗传分化.结果表明:79条序列共获得63个单倍型,3个群体呈现出高的单倍型多样性(Hd=0.991 56)和高的核苷酸多样性(Pi=0.026 81).Tajima'D检验、Fu's Fs检验和岐点分布检验(mismatch analysis)推测种群经历了群体扩张事件.单倍型邻接关系树和单倍型网络图表明澄黄滨珊瑚群体没有明显的地理谱系结构.分子方差分析(AMOVA)表明群体间遗传变异占1.22%,群体内遗传变异占98.78%.群体间固定指数(Fst)的绝对值为0.012 2,表明群体间遗传分化较弱.各群体间的基因流值均大于4,说明澄黄滨珊瑚在当地群体间随机交配,基因交流十分充沛,各珊瑚群体间没有地理隔离,可以互相作为造礁珊瑚修复用的来源补充地. | 牛文涛 田鹏 林荣澄 陈彬 Suharsono 施晓峰 郑新庆 | 2015 | 应用海洋学学报2015，34，3： | 1 |
| 17 | Impairment of tapetum and mitochondria in engineered male - sterile tobacco plants显示文摘 | Hernould M Suharsono S Zabaleta E | 1998 | Plant Mol Biol1998，36，4： | 1 |
| 18 | Male-sterility induction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat显示文摘 | Hernould M Suharsono S Litvaks | 1993 | Proc Natl Aead Sci USA1993，90，： | 1 |
| 19 | The heterotrimeric G protein alpha subunit acts upstream of the small GTPase Rac in disease resistance of rice 显示文摘 | Suharsono U Fujisawa Y Kawasaki T | 2002 | Proc Natl Acad Sci USA2002，99，13： | 1 |
| 20 | Male sterility in- duction in transgenic tobacco plants with an unedited atp9 mitochondrial gene from wheat 显示文摘 | Hernould M Suharsono S Litvak S | 1993 | Proceedings of the Na tional Academy of Sciences1993，90，： | 1 |
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