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| 1 | Differentiation of embryonic stem cells into insulin-producing cells promoted by Nkx2.2 gene transfer显示文摘AIM: To investigate the ability of a genetically altered embryonic stem (ES) cell line to generate insulin-producing cells in vitro following transfer of the Nkx2.2 gene.METHODS: Hamster Nkx2.2 genes were transferred into mouse ES cells. Parental and Nkx2.2-transfected ES cells were initiated toward differentiation in embryoid body (EB)culture for 5 d and the resulting EBs were transferred to an attached culture system. Dithizone (DTZ), a zincchelating agent known to selectively stain pancreatic beta cells, was used to detect insulin-producing cells.The outgrowths were incubated in DTZ solution (final concentration, 100 μg/mL) for 15 min before being examined microscopically. Gene expression of the endocrine pancreatic markers was also analyzed by RT-PCR. In addition, insulin production was determined immunohistochemically and its secretion was examined using an ELISA.RESULTS: DTZ-stained cellular clusters appeared after approximately 14 d in the culture of Nkx2.2-transfected ES cells (Nkx-ES cells), which was as much as 2 wk earlier, than those in the culture of parental ES cells (wt-ES). The frequency of DTZ-positive cells among total cultured cells on day 28 accounted for approximately 1.0% and 0.1% of the Nkx-ES- and wt-ES-derived EB outgrowths, respectively. The DTZ-positive cellular clusters were found to be immunoreactive to insulin, while the gene expressions of pancreatic-duodenal homeobox 1(PDX1), proinsulin 1 and proinsulin 2 were observed in the cultures that contained DTZ-positive cellular clusters.Insulin secretion was also confirmed by ELISA, whereas glucose-dependent secretion was not demonstrated.CONCLUSION: Nkx2.2-transfected ES cells showed an ability to differentiate into insulin-producing cells. | Akira Shiroi Shigehiko Ueda Yukiteru Ouji Ko Saito Kei Moriya Yuko Sugie Hiroshi Fukui Shigeaki Ishizaka Masahide Yoshikawa | 2005 | World Journal of Gastroenterology2005，11，27： | 9 |
| 2 | 颅内多发性动脉瘤患者术前破裂责任动脉瘤的判断显示文摘目的 探讨多发性颅内动脉瘤患者诊疗过程中误判破裂责任动脉瘤的原因和解决方法。方法对2003—2009年上海市浦东新区浦南医院收治的25例颅内多发性动脉瘤致自发性蛛网膜下腔出血(SAH)患者的临床资料进行回顾分析,术前根据Nehls等报道的评判原则诊断破裂责任动脉瘤,被确定破裂的责任动脉瘤均在48h内完成夹闭手术治疗。不能通过一次手术入路夹闭治疗的多发动脉瘤则远期进一步手术治疗。结果25例患者均通过手术证实破裂动脉瘤的确切部位,其中术中确认的责任破裂动脉瘤与术前诊断一致者20例(80%);而术前判断错误者4例(16%),且术后均发生再出血,其中2例因再出血死亡;还有1例诊断不明确。结论约80%左右的责任动脉瘤可通过术前CT、脑血管造影检查明确诊断,有疑议时应增加检查手段,如CTA或MRI等;术中需对责任动脉瘤进行确认;即使已经发现了责任动脉瘤,也要对其他部位的动脉瘤进行术中确认。 | 赵明珠 朱景伟 张宇一 钱忠心 王宇卉 Sugie Akira Kobata Hikoshi 刘卫东 | 2011 | 中国综合临床2011，27，2： | 3 |
| 3 | 主动脉夹层致急性缺血性脑卒中的诊治(附1例报告及文献复习)显示文摘目的总结主动脉夹层致急性缺血性脑卒中的诊断及治疗经验。方法回顾性分析1例主动脉夹层致急性缺血性脑卒中患者的临床资料,并结合文献进行分析。结果本例患者以急性缺血性脑卒中症状起病,急诊头颅MRI弥撒加权成像检查示左侧枕叶、颞叶后部早期缺血性改变;急诊胸部CT平扫及CT血管造影检查示Stanford A型胸主动脉解离伴血栓形成,同时伴左侧颈动脉和左侧锁骨下动脉血栓形成。保守治疗1月后,行血管重建手术治疗,移植人工血管置换解离的升主动脉且与双侧腋动脉搭桥。术后颈部磁共振血管造影检查示左侧颈动脉和左侧锁骨下动脉再通;放射性碘苯丙胺单光子放射计算机断层扫描检查示局部灌注改善。结论主动脉夹层致急性缺血性脑卒中的临床诊断需仔细鉴别,为改善脑血流动力学,血管重建治疗是一种有效的方法。 | 赵明珠 朱景伟 刘向阳 钱忠心 Sugie Akira Kobata Hikoshi 刘卫东 | 2014 | 中国临床神经外科杂志2014，19，12： | 3 |
| 4 | Increased prevalence of intestinal inflammation in patients with liver cirrhosis显示文摘AIM To investigate the pathophysiology of the digestive tract in patients with liver cirrhosis.METHODS In 42 cirrhotic patients and 20control subjects, the following fecal proteinswere measured by enzyme-linkedimmunosorb6nt assay: albumin (Alb ),transferrin (Tf), and al-antitrypsin (a,-AT) as amarker for intestinal protein l0ss, hemoglobin(Hb) for bleeding, PMN-eIastase for intestinalinflammation, and secretory lgA for intestinalimmunity.RESULTS The fecaI concentrations of Hb, Alb,Tf, al-AT, and PMN’eIastase were increased in13 (3l%), 8(19%), l0(24%), 6(14%), and 1l(26%) cases among 42 patients, resPectiveIy.F6cal concentration of secretory IgA wasdecreased in 7 (l7%) of 42 patients. However,these fecal concentrations were not related tothe severity or etiology of liver cirrhosis. Theserum Alb level was significantIy decreased inpatients with intestinal protein Ioss c0mpared tothat in patients without intestinal protein loss.CONCLUSION These findings suggest that: rob6sides the weIl’known pathological conditions,Such as bleeding and protein loss, intestinaIinflammati0n and decreased intestinaI immunityare found in cirrhotic patients, @ intestinalprotein loss contributes to hypoalbuminemia incirrhotic patients, and @ intestinaI inflammationshouId not b6 0verlooked in cirrhotic patients,since it may contribute to or cause intestinalprotein Ioss and oth6r various pathologicalconditions. | Saitoh O Sugi K Lojima K Matsumoto H Nakagawa K Kayazawa M Tanaka S Teranishi T Hirata I Katsu Ki K | 1999 | World Journal of Gastroenterology1999，5，5： | 2 |
| 5 | Combined assessment of T-wave alternans and late potentials used to predict arrhythmic events after myocardial infarction显示文摘 | Takanori Ikeda Takao Sakata Mitsuaki Takami Naoki Kondo Naoki Tezuka Takeshi Nakae Mahito Noro Yoshihisa Enjoji Ryoji Abe Kaoru Sugi Tetsu Yamaguchi | 2000 | Journal of the American College of Cardiology2000，，： | 2 |
| 6 | Obstacle avoidance of manipulators with rate constraints 显示文摘 | T Sugie K Fujimoto Y Kito | 2003 | IEEE Transactions on Robotics and Automation2003，19，1： | 1 |
| 7 | Linear-time connected-component labeling based on sequential local operations 显示文摘 | SUZUKI K HORIBA I SUGIE N | 2003 | Computer Vision and Image Understanding2003，89，1： | 1 |
| 8 | Sound localization in reverberant environment based on the model of the precedence effect显示文摘 | Huang J Ohnishi N Sugie N | 1997 | IEEE Transactions on Instrumentation and Measurement1997，46，4： | 1 |
| 9 | 显示文摘 | Sugi Y Tawada S Sugimura T | 1990 | Applied Catalysis A:General1990，189，： | 1 |
| 10 | Craniospinal irradiation using helical tomotherapy:evaluation of acute toxicity and dose distribution显示文摘 | Sugie C Shibamoto Y Ayakawa S | | 0，，02： | 1 |
| 11 | A new congenital form of X- linked autophagic vacuolar myopathy 显示文摘 | Yan C Tanaka M Sugie K | 2005 | Neurology2005，65，7： | 1 |
| 12 | Shape-selective alkylation of biphenyl over mordenites: Effects of dealumination on shape-selective and coke deposition显示文摘 | Sugi Y Matsuzaki T Hanaoka T | 1994 | Catal Lett1994，26，： | 1 |
| 13 | Intrathoracic bleed- ing during video-assisted thoracoscopic lobec-tomy and segmentectomy 显示文摘 | Sugi K Sudoh M Hirazawa K | 2003 | Japanese Journal of Thoracic Surgery2003，56，11： | 1 |
| 14 | Mesoporous Material from Zeolite显示文摘 | Y. Goto Y. Fukushima P. Ratu Y. Imada Y. Kubota Y. Sugi M. Ogura M. Matsukata | 2002 | Journal of Porous Materials2002，，1： | 1 |
| 15 | Genotoxicity of a variety of pyrrolizidine alkaloids in the hepatocyte primary culture-DNA re- pair test using rat,mouse,and h9msterhepatocytes显示文摘 | MORI H SUGIE S YOSHIMI N | 1985 | Cancer Res1985，45，7： | 1 |
| 16 | Shape-selective alkylation of biphenyl over mordenites: Cerium exchanged sodium mordenite and unmodified H-mordenite with low SiO2/Al2 O3 ratio显示文摘 | Sugi Y Matsuzaki T Hanaoka T | 1994 | Catal Lett1994，27，： | 1 |
| 17 | Mitochondrial alternative pathway is associated with development of freezing tolerance in common wheat显示文摘 | Mizuno N Sugie A Kobayashil F | 2007 | J Plant Physiol2007，，4： | 1 |
| 18 | An iterative learning control algorithm within prescribed input-output subspace显示文摘 | Hamamoto K Sugie T | 2001 | Automatica2001，37，11： | 1 |
| 19 | An iterative learning control law for dynamical systems 显示文摘 | Sugie T and Ono T | 1991 | Automatica1991，27，4： | 1 |
| 20 | E7070, a novel sulphonamide agent with potent antitumour activity in vitro and in vivo 显示文摘 | Ozawa Y Sugi NH Nagasu T | 2001 | Ear J Cancer2001，37，17： | 1 |
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