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| 1 | Characterization of hepatic progenitors from human fetal liver during second trimester显示文摘AIM: To enrich hepatic progenitors using epithelial cell adhesion molecule (EpCAM) as a marker from human fetal liver and investigate the expression of human leukocyte antigen (HLA) and their markers associated with hepatic progenitor cells. METHODS: EpCAM +ve cells were isolated using magnetic cell sorting (MACS) from human fetuses (n = 10) at 15-25 wk gestation. Expression of markers for hepatic progenitors such as albumin, alpha-fetoprotein (AFP), CD29 (integrin β1), CD49f (integrin α6) and CD90 (Thy 1) was studied by using flow cytometry, immunocytochemistry and RT-PCR; HLA class Ⅰ (A, B, C) and class Ⅱ (DR) expression was studied by flow cytometry only. RESULTS: FACS analysis indicated that EpCAM +ve cells were positive for CD29, CD49f, CD90, CD34, HLA class Ⅰ, albumin and AFP but negative for HLA class Ⅱ (DR) and CD45. RT PCR showed that EpCAM +ve cells expressed liver epithelial markers (CK18), biliary specific marker (CK19) and hepatic markers (albumin, AFP). On immunocytochemical staining, EpCAM +ve cells were shown positive signals for CK18 and albumin. CONCLUSION: Our study suggests that these EpCAM +ve cells can be used as hepatic progenitors for cell transplantation with a minimum risk of alloreactivity and these cells may serve as a potential source for enrichment of hepatic progenitor. | Mekala Subba Rao Aleem Ahmed Khan Nyamath Parveen Mohammed Aejaz Habeeb Chittoor Mohammed Habibullah Gopal Pande | 2008 | World Journal of Gastroenterology2008，14，37： | 16 |
| 2 | Flotation studies on low grade graphite ore from eastern India显示文摘A low grade graphite ore from eastern India was beneficiated by flotation to improve its quality. The ore was composed of 87.80% ash and 8.59% fixed carbon. Primary coarse wet grinding(d80: 186 lm) followed by rougher flotation in Denver flotation cell using diesel as collector and pine oil as frother yielded a rougher concentrate. Regrinding(d80: 144 lm) of this rougher concentrate was opted for further liberation of graphite. It was followed by cleaning in laboratory flotation column. This combined process of relatively coarse primary grinding followed by regrinding and cleaning in flotation column resulted in final concentrate of 7.44% yield with 89.65% fixed carbon and 6.00% ash. This approach of two-stage grinding to recover the flake graphite at the coarsest possible grind can help to minimize grinding energy costs. A conceptual flow sheet which is cost effective was developed based on this methodology. | Vasumathi N. Vijaya Kumar T.V. Ratchambigai S. Subba Rao S. Bhaskar Raju G. | 2015 | International Journal of Mining Science and Technology2015，25，3： | 6 |
| 3 | Beneficiation of an Indian non-coking coal by column flotation显示文摘 | N. Vasumathi T. V. Vijaya Kumar S. Ratchambigai S. Subba Rao S. Prabhakar G. Bhaskar Raju | 2016 | International Journal of Coal Science & Technology2016，3，2： | 5 |
| 4 | Thinking outside the liver: Induced pluripotent stem cells for hepatic applications显示文摘The discovery of induced pluripotent stem cells (iPSCs) unraveled a mystery in stem cell research, after identification of four re-programming factors for generating pluripotent stem cells without the need of embryos. This breakthrough in generating iPSCs from somatic cells has overcome the ethical issues and immune rejection involved in the use of human embryonic stem cells. Hence, iPSCs form a great potential source for developing disease models, drug toxicity screening and cell-based therapies. These cells have the potential to differentiate into desired cell types, including hepatocytes, under in vitro as well as under in vivo conditions given the proper microenvironment. iPSC-derived hepatocytes could be useful as an unlimited source, which can be utilized in disease modeling, drug toxicity testing and producing autologous cell therapies that would avoid immune rejection and enable correction of gene defects prior to cell transplantation. In this review, we discuss the induction methods, role of reprogramming factors, and characterization of iPSCs, along with hepatocyte differentiation from iPSCs and potential applications. Further, we discuss the location and detection of liver stem cells and their role in liver regeneration. Although tumor formation and genetic mutations are a cause of concern, iPSCs still form a promising source for clinical applications. | Mekala Subba Rao Mitnala Sasikala D Nageshwar Reddy | 2013 | World Journal of Gastroenterology2013，19，22： | 4 |
| 5 | CeCl_3·7H_2O-SiO_2:Catalyst promoted microwave assisted neat synthesis,antifungal and antioxidant activities of α-diaminophosphonates显示文摘CeCl3 汃″ ??? 伲匭佩 ? ? 賥芉铦膌? ?? ? 諥?? 蒚郥邈 ? 鞊鳧? 貏 ? 钸 ? 楤浡湩灯潨灳潨慮整 ? 髧鞊 ? 隌觥? 諥¨ ?? 泔?? | Subba Rao Devineni Srinivasulu Doddaga Rajasekhar Donka Naga Raju Chamarthi | 2013 | Chinese Chemical Letters2013，24，8： | 3 |
| 6 | Performance of layered Li(Ni 1/3 Co 1/3 Mn 1/3 )O 2 as cathode for Li-ion batteries显示文摘 | K.M Shaju G.V Subba Rao B.V.R Chowdari | 2002 | Electrochimica Acta2002，，2： | 2 |
| 7 | Studies on the Functional Properties of Protein Concentrate of Kappaphycus alvarezii (Doty) Doty – An Edible Seaweed显示文摘 | K. Suresh Kumar K. Ganesan Kandasamy Selvaraj P.V. Subba Rao | 2013 | Food Chemistry2013，，： | 2 |
| 8 | Synthesis,isolation,identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPLC,LC/ESI-MS and NMR显示文摘One unknown impurity(Imp-Ⅱ) during the analysis of laboratory batches of isoproterenol hydrochloride was detected in the level ranging from 0.04% to 0.12% by high performance liquid chromatography with UV detection. The unknown impurity structure was proposed as 4-[2-(propan-2-ylamino)ethyl]benzene-1,2-diol(Imp-Ⅱ) using the liquid chromatography–mass spectrophotometry(LC–MS) analysis. Imp-Ⅱ was isolated by semi-preparative liquid chromatography from the impurity-enriched reaction crude sample. Its proposed structure was confirmed by nuclear magnetic spectroscopy such as ~1H,^(13)C, DEPT(1D NMR), HSQC(2D NMR)and infrared spectroscopy(IR), and retention time and purity with HPLC followed by the chemical synthesis.Due to less removable nature of Imp-Ⅱ during the purification, the synthetic process was optimized proficiently to control the formation of Imp-Ⅱ below to the limit < 0.12% in the course of reaction. The new chemical route was developed for the preparation of this impurity in required quantity with purity to use as reference standard.The most probable mechanism for the formation of Imp-Ⅱ was discussed in details. | Neeraj Kumar Subba Rao Devineni Prasad Reddy Gajjala Shailendra Kumar Dubey Pramod Kumar | 2017 | Journal of Pharmaceutical Analysis2017，7，6： | 2 |
| 9 | Effectiveness of sodium silicate as gangue depressants in iron ore slimes flotation显示文摘The recovery of iron from the screw classifier overflow slimes by direct flotation was studied.The relative effectiveness of sodium silicates with different silica-to-soda mole ratios as depressants for silica and silicate bearing minerals was investigated.Silica-to-soda mole ratio and silicate dosage were found to have significant effect on the separation efficiency.The results show that an increase of Fe content in the concentrate is observed with concomitant reduction in SiO2 and Al2O3 levels when a particular type of sodium silicate at a proper dosage is used.The concentrate of 58.89wt% Fe,4.68wt% SiO2,and 5.28wt% Al2O3 with the weight recovery of 38.74% and the metal recovery of 41.13% can be obtained from the iron ore slimes with 54.44wt% Fe,6.72wt% SiO2,and 6.80wt% Al2O3,when Na2SiO3 with a silica-to-soda mole ratio of 2.19 is used as a depressant at a feed rate of 0.2 kg/t. | Danda Srinivas Rao Tadiparthi Venkata VijayaKumar Sripada Subba Rao Swarna Prabhakar Guntamadugu Bhaskar Raju | 2011 | International Journal of Minerals,Metallurgy and Materials2011，18，5： | 2 |
| 10 | Geochemistry of adakites and rhyolites from the Neoarchean Gadwal greenstone belt,eastern Dharwar Craton,India:implications for sources and geodynamic setting显示文摘 | Manikyamba C Kerrich R Khanna T.C Subba Rao D.V | | 0，，： | 1 |
| 11 | Swellingbehavior of desiccated clay显示文摘 | SUBBA K S RAO S M GANGADHARA S | 2002 | ASTM GeotechnicalTesting Journal2002，23，2： | 1 |
| 12 | Per- formanee of layered Li ( Ni1/3 Co1/3 Mn1/3 ) 02 as cath- ode for L-ion batteries 显示文摘 | K M Shaju G V Subba Rao B V R Chowdari | 2002 | Electrochimiea Acta2002，48，2： | 1 |
| 13 | Electro-chemical properties of carbon-coated CaW04 versus Li显示文摘 | SHARMA N SUBBA RAO G V CHOWDARI B V R | 2005 | Elec-trochim Acta2005，50，27： | 1 |
| 14 | Performance of layered Li(Ni1/3Co1/3Mn1/3)O2 as cathode for Li-ion batteries显示文摘 | SHAJU K M SUBBA RAO G V CHOWDARI B V R | 2002 | Electrochim Acta2002，48，： | 1 |
| 15 | A study of solvents for sodium borohydride and the effect of solvent and the metal ion on borohydride reductions显示文摘 | Brown H C Mead E J Subba Rao B C | 1955 | Journal of the American Chemical Society1955，77，23： | 1 |
| 16 | Chemical characteristics of groundwater and assessment of groundwater quality in Varaha River Basin, Visakhapatnam District, Andhra Pradesh, India显示文摘 | SUBBA RAO N SURYA RAO P VENKTRAM REDDY G | 2012 | Environmental Monitoring and Assessment2012，184，8： | 1 |
| 17 | Metal oxides and oxysalts as anode materials for Li ion batteries 显示文摘 | REDDY M V SUBBA RAO G V CHOWDARI B V R | 2013 | Chem Rev2013，113，7： | 1 |
| 18 | Seismic passive earth pressures in soils显示文摘 | SUBBA RAO K S CHOUDHURY D | 2005 | Journal of Geotechnical and Geoenvironmental Engineering2005，131，1： | 1 |
| 19 | Seismic passive earth pressures insoils显示文摘 | Subba Rao K S Choudhury D | 2005 | Journal of Geotechnical and Geo-environmental Engineer-ing2005，131，1： | 1 |
| 20 | Antioxidant potential of solvent extracts of kappaphycus alvarezii (doty) doty-anedible seaweed 显示文摘 | Suresh Kumar K Ganesan K Subba Rao P V | 2008 | Food Chem2008，107，1： | 1 |
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