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| 1 | Claudin-11 and occludin are major contributors to Sertoli cell tight junction function, in vitro显示文摘Sertoli 房间紧密的连接(TJ ) 是血睾丸障碍的关键部件,在它扣押开发在生精的小管以内经历精子发生的细菌房间的地方。神经质地调整的 claudin-11 是在不孕涉及障碍功能和它的鼠科的猛烈结果的批评 transmembrane 蛋白质。我们试图估计份量上到 TJ 功能的 claudin-11 的贡献的意义在 vitro,使用调停 siRNA 的基因 silencing。我们也进行了 occludin 的贡献的分析, TJ 的另一内在的 transmembrane 蛋白质。claudin-11 或 occludin 的 Silencing 在一个不成熟的老鼠 Sertoli 房间文化模型用 siRNA 被进行。Transepithelial 电的抵抗被用来在整个文化估计份量上 TJ 功能。在 siRNA 处理以后的二天,房间为对 mRNA 的 RT-PCR 评价为连接蛋白质或 lyzed 的 immunocytochemical 本地化被修理表示。claudin-11, occludin,或两个的 Silencing 在 55% 的 TJ 功能导致了重要减少(P <0.01 ) , 51%(P <0.01 ) ,并且 62%(P <0.01 ) 分别地。数据在到 Sertoli 房间 TJ 的指向的蛋白质的本地化是有在 mRNA 表示和显著减小的重要减少的伴随物。我们提供 claudin-11 显著地贡献的量的证据(P <0.01 ) 到 Sertoli 房间 TJ 在 vitro 工作。有趣地, occludin,神经质地在不孕被调整然而并非含有直到迟了的成人生活,也是一重要(P <0.01 ) 贡献者到障碍功能。我们的数据与一致在清楚地为这些表明一个角色的 vivo 研究,在维持正常 TJ 障碍的蛋白质组织并且工作。 | Mark J McCabe Caroline FH Foo Marcel E Dinger Peter M Smooker Peter G Stanton | 2016 | Asian Journal of Andrology2016，18，4： | 7 |
| 2 | Distribution andgenetic diversity of lactic acid bacteria from traditional fermentedsausage显示文摘 | TRAN KTM MAY B E SMOOKER P M et ai | 2011 | Food Research International2011，44，1： | 1 |
| 3 | For The Record: A Single Amino Acid Substitution Affects Substrate Specificity In Cysteine Proteinases From Fasciola Hepatica显示文摘 | Smooker P M Whisstock J C Irving J A | 2008 | Protein Sci2008，9，12： | 1 |
| 4 | Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection显示文摘 | Law R H P Smooker P H Irving J A | | 0，，12： | 1 |
| 5 | The antibiotic resistance characteristics of non-typhoidal Salmonella enterica isolated from food-producing animals, retail meat and humans in South East Asia显示文摘 | Thi Thu Hao Van Hoang Nam Kha Nguyen Peter M. Smooker Peter J. Coloe | 2011 | International Journal of Food Microbiology2011，，3： | 1 |
| 6 | Expression library immunization protects mice against a challenge with virulent rodent malaria显示文摘 | Smooker PM Setiady YY Rainczuk A | 2000 | Vaccine2000，18，23： | 1 |
| 7 | Evaluation of antigens of Fasciola gigantica as vaccines against tropical fasciolosis in cattle 显示文摘 | Estuningsih E S Smooker P M Wiedosari E | 1997 | Int J parasitol1997，27，： | 1 |
| 8 | Evaluation of antigens of Fasciola gigantica as vaccines against tropical fasciolosis in cattle显示文摘 | Estuningsih E S Smooker P M Wiedosari E | 1997 | Int J paras1997，27，： | 1 |
| 9 | Cathepsin B proteases of flukes: the key to facilitating parasite control? 显示文摘 | Smooker PM Jayaraj R Pike RN | 2010 | Trends Parasi- tol2010，26，10： | 1 |
| 10 | Distribution and genetic diversity of lactic acid bacteria from traditional fermented sausage显示文摘 | TRAN K TM MAY B K SMOOKER P M | 2011 | Food Research International2011，44，1： | 1 |
| 11 | Induction of specific T-cell responses,opsonizing antibodies,and protection against plasmodium chabaudi adami infection in mice vaccinated with genomic expression libraries expressed in targeted and secretary DNA vectors显示文摘 | Rainczuk A Scorza T Smooker P M | 2003 | Infect Immun2003，71，： | 1 |
| 12 | Cloning and expression of the major secreted cathepsin B-like protein from juvenile Fasciola hepatica and analysis of immunogenicity following liver fluke infection显示文摘 | Law RH Smooker PM Irving JA | 2003 | Infect Immun2003，71，12： | 1 |
| 13 | Immunological approaches for the control of fasciolosis 显示文摘 | Spithill TW Piedrafita D Smooker P M | 1997 | Int J Parasitol1997，27，10： | 1 |
| 14 | Distribution and ge-netic diversity of lactic acid bacteria from traditional fer- mented sausage 显示文摘 | Tran K May B K Smooker P M | 2011 | Food Research International2011，44，1： | 1 |
| 15 | The antibiotic resistance characteristics of non-typhoidal Salmonella enterica isolated from food-producing animals,retail meat and humans in South East Asia显示文摘 | Van T H Nguyen N K Smooker P M | 2012 | International Journal of Food Microbiology2012，154，3： | 1 |
| 16 | Delivery of a heterologous antigen by a registered Salmonella vaccine (STM1)显示文摘 | Endang W Bachtiar Kuo-Ching Sheng Theodora Fifis Anita Gamvrellis Magdalena Plebanski Peter J Coloe Peter M Smooker | 2003 | FEMS Microbiology Letters2003，，： | 1 |
| 17 | Immunological approaches for the control of fasciolosis 显示文摘 | Spithill TW Piedrafita D Smooker PM | 1997 | Int J Parasitol1997，27，： | 1 |
| 18 | The protective efficacy of MSP4/5 against lethal Plasmodium chabaudi adami challenge is dependent on the type of DNA vaccine vector and vaccination protocol显示文摘 | A Rainczuk P.M Smooker L Kedzierski C.G Black R.L Coppel T.W Spithill | 2003 | Vaccine2003，，： | 1 |
| 19 | Expression library immunization protects mice against a challenge with virulent rodent malaria 显示文摘 | Smooker PM Setlady YY Rainczuk A | 2000 | Vaccine2000，18，2： | 1 |
| 20 | 用酶联免疫吸附试验诊断蓝氏贾第鞭毛虫病免疫价值的研究(英文)显示文摘 用蓝氏贾第鞭毛虫纯培养虫株和超声粉碎可溶性虫体部分作为抗原,采用ELISA方法检测人体感染蓝氏贾第鞭毛虫血清免疫抗体并讨论其寄生虫学诊断价值.检测65份成人贾第虫病血清,其中55份IgG和/或IgA阳性,阳性率84.62%.20份10岁以下儿童贾第虫病人血清无1份阳性.结果提示蓝氏贾第鞭毛虫感染产生的体液免疫反应存在有年龄依赖性,酶联免疫吸附试验用于蓝氏贾第鞭毛虫病免疫诊断具有局限性. | 陈思礼 陈强 袁媛 李玲 陈思义 廖林贵 SPITHILL Terry SMOOKER Peter | 2004 | 华中师范大学学报(自然科学版)2004，38，4： | 1 |
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